LOCUS       sequence-269047-       13445 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  overexpression in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13445)
  AUTHORS   Yang LZ, Wang Y, Li SQ, Yao RW, Luan PF, Wu H, Carmichael GG, Chen 
            LL
  TITLE     Dynamic Imaging of RNA in Living Cells by CRISPR-Cas13 Systems.
  JOURNAL   Mol Cell. 2019 Dec 19;76(6):981-997.e7. doi: 
            10.1016/j.molcel.2019.10.024. Epub 2019 Nov 19.
  PUBMED    31757757
REFERENCE   2  (bases 1 to 13445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2019.10.024"; journalName: "Mol Cell"; date: 
            "2019-12-19- 19"; volume: "76"; issue: "6"; pages: "981-997.e7"
COMMENT     Sequence Label: pHAGE-IRES-puro-NLS-dLbaCas13a-EGFP-NLS-3xFlag
FEATURES             Location/Qualifiers
     source          1..13445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3414..3461
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     regulatory      7800..7809
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7806..7808,7809..7811,7812..8522)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7806..7808,7809..7811,7812..8522)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7851..7872)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     8459..8480
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8550..8597
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8616..8681
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    8688..9255
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8849..8866)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9076..9095
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             9275..9874
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9275..9294)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9771..9791
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9893..10481
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9946..9966)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10364..10375)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10556..10789
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10908..10926)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10994..11098
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11099..11167,11168..11959)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11317..11336)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12130..12718
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12619..12638
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12872..12889
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     12981..13000
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     protein_bind    13071..13092
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13107..13112,13113..13130,13131..13137)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13145..13161
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13150..13172
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13169..13185
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13169..13185
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13235..13254
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc atgaagcgac
     3421 ctgccgccac aaagaaggct ggacaggcta agaagaagaa agctagcatg aagatcagca
     3481 aggtgcggga ggagaacaga ggcgccaagc tgaccgtgaa tgccaagaca gccgtggtgt
     3541 ctgagaaccg gagccaggag ggcatcctgt ataatgaccc aagcaggtac ggcaagtccc
     3601 gcaagaacga cgaggatagg gaccgctata tcgagagcag actgaagagc tccggcaagc
     3661 tgtaccggat ctttaacgag gataagaata agcgcgagac agacgagctg cagtggttcc
     3721 tgtccgagat cgtgaagaag atcaaccgga gaaatggcct ggtgctgtcc gacatgctgt
     3781 ctgtggacga tagagccttc gagaaggcct ttgagaagta cgccgagctg tcttatacca
     3841 acaggcgcaa taaggtgtcc ggctctcccg ccttcgagac atgcggagtg gatgcagcaa
     3901 cagcagagcg gctgaaggga atcatctccg agacaaactt catcaacaga atcaagaaca
     3961 acatcgacaa caaggtgtct gaggacatca tcgatcggat catcgccaag tacctgaaga
     4021 agagcctgtg ccgggagaga gtgaagagag gcctgaagaa gctgctgatg aatgcctttg
     4081 atctgccata ctctgatccc gacatcgatg tgcagaggga cttcatcgat tatgtgctgg
     4141 aggactttta ccacgtgcgg gccaagagcc aggtgagcag atccatcaag aacatgaata
     4201 tgcccgtgca gcctgagggc gacggcaagt tcgccatcac cgtgagcaag ggcggcacag
     4261 agtctggcaa caagcgcagc gccgagaagg aggccttcaa gaagtttctg agcgattacg
     4321 cctccctgga cgagagggtg cgcgacgata tgctgcggag aatgaggcgc ctggtggtgc
     4381 tgtactttta tggctccgac gattctaagc tgagcgatgt gaacgagaag ttcgacgtgt
     4441 gggaggacca cgcagcccgg agagtggaca atagggagtt catcaagctg ccactggaga
     4501 acaagctggc caatggcaag accgacaagg atgccgagcg gatcagaaag aacacagtga
     4561 aggagctgta tagaaaccag aatatcggct gctacaggca ggccgtgaag gccgtggagg
     4621 aggacaacaa tggccggtac tttgacgata agatgctgaa catgttcttt atccacagaa
     4681 tcgagtatgg cgtggagaag atctacgcca atctgaagca ggtgaccgag ttcaaggccc
     4741 gcacaggcta cctgagcgag aagatctgga aggatctgat caactacatc tctatcaagt
     4801 atatcgccat gggcaaggcc gtgtataact atgccatgga tgagctgaat gcctccgaca
     4861 agaaggagat cgagctgggc aagatctccg aggagtatct gtctggcatc tctagcttcg
     4921 actacgagct gatcaaggcc gaggagatgc tgcagaggga gacagccgtg tacgtggcct
     4981 ttgccgcaag gcacctgtcc tctcagacag tggagctgga ttccgagaac tctgacttcc
     5041 tgctgctgaa gcctaagggc accatggaca agaacgataa gaataagctg gcctccaaca
     5101 atatcctgaa ttttctgaag gataaggaga cactgcggga cacaatcctg cagtatttcg
     5161 gcggccactc tctgtggaca gatttcccat ttgacaagta cctggccggc ggcaaggacg
     5221 atgtggattt tctgaccgac ctgaaggatg tgatctatag catggcgaac gactccttcg
     5281 cctacgccac agagaaccac aacaatggca agtggaataa ggagctgatc tccgccatgt
     5341 ttgagcacga gacagagaga atgacagtgg tcatgaagga caagttctat tctaacaatc
     5401 tgcccatgtt ttacaagaac gacgatctga agaagctgct gatcgacctg tataaggaca
     5461 atgtggagag agcctctcag gtgcccagct tcaacaaggt gtttgtgcgc aagaatttcc
     5521 ctgccctggt gcgggacaag gataacctgg gcatcgagct ggatctgaag gccgacgccg
     5581 ataagggcga gaatgagctg aagttctaca acgccctgta ctacatgttc aaggagatct
     5641 actacaacgc cttcctgaac gacaagaatg tgagggagcg gttcatcacc aaggccacaa
     5701 aggtggccga caactatgat aggaataagg agcgcaacct gaaggatagg atcaagagcg
     5761 ccggctccga cgagaagaag aagctgcgcg agcagctgca gaattacatc gccgagaacg
     5821 atttcggcca gaggatcaag aatatcgtgc aggtgaaccc tgactatacc ctggcccaga
     5881 tctgccagct gatcatgaca gagtacaacc agcagaacaa tggctgtatg cagaagaaga
     5941 gcgccgcccg gaaggatatc aataaggact cctaccagca ctataagatg ctgctgctgg
     6001 tgaacctgag aaaggccttc ctggagttta tcaaggagaa ttatgccttt gtgctgaagc
     6061 cctacaagca cgacctgtgc gataaggccg acttcgtgcc tgattttgcc aagtacgtga
     6121 agccctacgc cggcctgatc agcagggtgg caggcagctc cgagctgcag aagtggtata
     6181 tcgtgtcccg ctttctgtct cctgcccagg ccaaccacat gctgggcttc ctgcactcct
     6241 acaagcagta cgtgtgggac atctacaggc gcgcctctga gacaggcacc gagatcaatc
     6301 acagcatcgc cgaggataag atcgccggcg tggacatcac cgacgtggat gccgtgatcg
     6361 atctgagcgt gaagctgtgc ggcacaatct ctagcgagat ctccgactac ttcaaggacg
     6421 atgaggtgta cgccgagtat atctcctctt acctggattt tgagtatgac ggcggcaact
     6481 acaaggatag cctgaatagg ttctgtaact ccgatgccgt gaatgaccag aaggtggccc
     6541 tgtactatga cggcgagcac cctaagctga accgcaatat catcctgagc aagctgtacg
     6601 gcgagcggag atttctggag aagatcaccg atcgggtgag ccggagcgac atcgtggagt
     6661 actataagct gaagaaggag acaagccagt accagacaaa gggcatcttc gatagcgagg
     6721 acgagcagaa gaacatcaag aagtttcagg agatgaagaa tatcgtggag ttcagagatc
     6781 tgatggacta tagcgagatc gccgatgagc tgcagggcca gctgatcaac tggatctacc
     6841 tgcgggagcg ggacctgatg aatttccagc tgggctacca ctatgcctgc ctgaacaatg
     6901 attccaacaa gcaggccacc tatgtgacac tggactacca gggcaagaag aatcggaaga
     6961 tcaacggcgc catcctgtat cagatctgtg ccatgtatat caatggcctg cctctgtact
     7021 atgtggacaa ggatagctcc gagtggaccg tgtctgacgg caaggagagc acaggcgcca
     7081 agatcggcga gttctacaga tatgccaagt cctttgagaa cacctctgat tgctacgcca
     7141 gcggcctgga gatctttgag aatatcagcg agcacgacaa catcacagag ctggccaatt
     7201 atatcgaggc ctttcgctac tacagcagct tcgaccggag cttcctgggc atctactctg
     7261 aggtgttcga tcggttcttt acctacgacc tgaagtatag aaagaacgtg ccaacaatcc
     7321 tgtataacat cctgctgcag cacttcgtga acgtgaggtt cgagttcgtg agcggcaaga
     7381 agatgatcgg catcgataag aaggaccgca agatcgccaa ggagaaggag tgtgcccgga
     7441 tcaccatcag agagaagaac ggcgtgtata gcgagcagtt tacctacaag ctgaagaatg
     7501 gcacagtgta tgtggatgcc agggacaaga gatacctgca gtccatcatc aggctgctgt
     7561 tctatccaga gaaggtgaac atggatgaga tgatcgaggt gaaggagaag aagaagccct
     7621 ccgacaacaa taccggcaag ggctactcta agagggatcg ccagcaggac cgcaaggagt
     7681 acgataagta taaggagaag aagaagaagg agggcaattt cctgagcggc atgggcggca
     7741 acatcaattg ggacgagatc aatgcccagc tgaagaacag tctagaggcg ctaccggtcg
     7801 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     7861 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     7921 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     7981 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     8041 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     8101 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     8161 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     8221 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     8281 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     8341 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     8401 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     8461 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     8521 agtcgaattc taagccagga ttccatatga agcgacctgc cgccacaaag aaggctggac
     8581 aggctaagaa gaagaaacaa cgcgtgcagc taagcgacta caaagaccat gacggtgatt
     8641 ataaagatca tgacatcgat tacaaggatg acgatgacaa gtaaggatcc ctcccccccc
     8701 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     8761 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     8821 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     8881 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     8941 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     9001 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     9061 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     9121 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
     9181 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
     9241 aaaacacgat gataaggatc caccggagct taccatgacc gagtacaagc ccacggtgcg
     9301 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     9361 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     9421 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     9481 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     9541 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     9601 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     9661 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     9721 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     9781 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     9841 cacctggtgc atgacccgca agcccggtgc ctgacgccca tcgatagatc ctaatcaacc
     9901 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     9961 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
    10021 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
    10081 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
    10141 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
    10201 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
    10261 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
    10321 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
    10381 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
    10441 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgagatcc tttaagacca
    10501 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
    10561 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
    10621 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
    10681 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
    10741 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
    10801 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
    10861 cccgggttaa ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc tcgtgatacg
    10921 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
    10981 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
    11041 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
    11101 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
    11161 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
    11221 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
    11281 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
    11341 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
    11401 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
    11461 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
    11521 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
    11581 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
    11641 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
    11701 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
    11761 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
    11821 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
    11881 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
    11941 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
    12001 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
    12061 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    12121 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
    12181 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
    12241 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
    12301 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    12361 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    12421 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    12481 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    12541 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    12601 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    12661 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    12721 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
    12781 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
    12841 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
    12901 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagcaagct
    12961 catggctgac taattttttt tatttatgca gaggccgagg cttttgcaaa aagctccccg
    13021 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
    13081 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
    13141 ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat tacgaatttc
    13201 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
    13261 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
    13321 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
    13381 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
    13441 gtagt
//
