LOCUS       sequence-269048-       10819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of intradomain insertion of truncated human cytosine 
            deaminase, hAIDx, at position 468 of nSpyCas9 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10819)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 10819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: AIDx-ID-468
FEATURES             Location/Qualifiers
     source          1..10819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        142..521
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        522..725
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     676..696
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     770..789
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        770..788
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      824..833
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2234..2254
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2270..2290
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5675..5722
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5723..5746
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5756..5812
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(6317..6334)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6323..6547
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6593..7021
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6680..6699)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6890..6911
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7030..7050)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7035..7364
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7215..7350
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7277..7296
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7431..8225
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7485..7504)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8095..8114
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8399..8520
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8436..8455)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8490..8509
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8569..8585)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8569..8585)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8582..8604)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8593..8609
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8617..8623,8624..8641,8642..8647))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8662..8683
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8800..8817)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8971..9559)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9051..9070)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9730..10521,10522..10590))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10353..10372
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10591..10695)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10770..10789)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
       61 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      121 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      181 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      241 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      301 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      361 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      421 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      481 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      541 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      601 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      661 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      721 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      781 actataggga gacccaagct ggctagcgtt taaacttaag cttgccacca tggacaaaaa
      841 atatagcatt ggcctggcga ttggcaccaa ctccgtgggc tgggctgtta tcaccgacga
      901 atacaaggtt ccgagcaaga aattcaaggt gctgggtaac accgaccgcc acagcatcaa
      961 gaaaaacctg attggtgccc tgctgtttga tagcggtgaa accgccgagg ctacccgtct
     1021 gaagcgcacc gcccgtcgcc gttatacccg tcgtaaaaac cgtatctgct acctgcaaga
     1081 gattttcagc aacgaaatgg ccaaagtgga cgatagcttc tttcaccgcc tggaggagag
     1141 cttcctggtg gaggaagaca agaaacacga gcgtcacccg atctttggca acattgtgga
     1201 tgaggtggct tatcacgaaa agtacccgac catctatcac ctgcgtaaga aactggtgga
     1261 tagcaccgac aaggccgatc tgcgcctgat ctacctggcg ctggctcaca tgatcaagtt
     1321 ccgtggtcac tttctgatcg agggcgacct gaacccggat aacagcgatg tggacaagct
     1381 gttcatccag ctggtgcaga cctacaacca gctgtttgag gaaaacccga ttaacgccag
     1441 cggtgtggac gccaaggcga tcctgagcgc gcgtctgagc aaaagccgcc gtctggagaa
     1501 cctgatcgcg cagctgccgg gtgaaaagaa aaacggtctg ttcggcaacc tgattgctct
     1561 gagcctgggc ctgaccccga acttcaaaag caactttgac ctggccgagg atgcgaagct
     1621 gcaactgagc aaggatacct acgacgatga cctggacaac ctgctggcgc agatcggcga
     1681 ccagtatgct gatctgtttc tggccgcgaa gaacctgagc gatgcgatcc tgctgagcga
     1741 cattctgcgt gtgaacaccg agattaccaa ggctccgctg agcgccagca tgatcaaacg
     1801 ctacgatgaa caccaccagg acctgaccct gctgaaagct ctggtgcgtc agcagctgcc
     1861 ggagaagtat aaagaaatct tctttgacca gagcaagaac ggttacgccg gctatattga
     1921 tggcggtgcg agccaggagg agttctacaa gtttatcaaa ccgattctgg agaaaatgga
     1981 cggcaccgag gaactgctgg tgaagctgaa ccgtgaagat ctgctgcgta aacagcgcac
     2041 cttcgacaac ggtagcatcc cgcaccagat tcacctgggt gagctgcacg cgatcctgcg
     2101 ccgtcaggaa gatttctacc cgtttctgaa ggacaaccgt gagaagatcg aaaaaattct
     2161 gaccttccgc attccgtact atgtgggtcc gctggctcgt ggcaacagcc gctttgcctg
     2221 gatgacccgt aaaccgaaga aaaagcgcaa agtgggtagc agcggcagcc cgaaaaagaa
     2281 acgcaaagtg ggtagcgacg ccctggatga cttcgatctg gacatgctgg gcagcgacgc
     2341 gctggatgac tttggcggtg gcagcatgga tagcctgctg atgaaccgcc gtaaattcct
     2401 gtaccagttt aagaacgtgc gttgggctaa aggccgccgt gaaacctacc tgtgctatgt
     2461 ggtgaaacgc cgtgatagcg ccaccagctt cagcctggac tttggttatc tgcgcaacaa
     2521 gaacggctgc cacgtggagc tgctgttcct gcgttatatt agcgattggg acctggaccc
     2581 aggccgttgc taccgtgtga cctggttcac cagctggagc ccgtgctatg attgcgcgcg
     2641 tcacgtggct gactttctgc gcggcaaccc gaacctgagc ctgcgtatct tcaccgcgcg
     2701 cctgtacttt tgcgaggacc gtaaggctga gccggaaggt ctgcgccgtc tgcaccgtgc
     2761 gggtgtgcag atcgcgatca tgaccttcaa agattacttt tattgctgga acaccttcgt
     2821 ggagaaccac gaacgtacct ttaaggcctg ggagggtctg cacgaaaaca gcgtgcgtct
     2881 gagccgccag ctgcgccgta tcctgctgcc gggtggcagc ggtggcagcg gtggcagcgg
     2941 tggcagcggt ggcagcggtg gcagcggtgg caagagcgag gaaaccatca ccccgtggaa
     3001 cttcgaggaa gtggtggaca aaggcgccag cgcccagagc ttcattgagc gtatgaccaa
     3061 ctttgataag aacctgccga acgaaaaagt gctgccgaag cacagcctgc tgtacgagta
     3121 cttcaccgtg tacaacgaac tgaccaaggt gaaatacgtg accgagggta tgcgcaagcc
     3181 ggccttcctg agcggcgaac agaagaaagc gatcgtggac ctgctgttta aaaccaaccg
     3241 taaggtgacc gtgaagcagc tgaaagagga ttacttcaag aaaattgaat gcttcgacag
     3301 cgtggagatc agcggtgtgg aagatcgttt caacgcgagc ctgggcacct atcacgacct
     3361 gctgaaaatc attaaggaca aggattttct ggataacgag gaaaacgagg acatcctgga
     3421 agatattgtg ctgaccctga ccctgttcga ggaccgtgaa atgatcgagg aacgcctgaa
     3481 gacctacgcc cacctgtttg atgacaaagt gatgaaacag ctgaagcgcc gtcgctatac
     3541 cggttggggc cgtctgagcc gcaaactgat caacggtatt cgcgataaac agagcggcaa
     3601 gaccatcctg gatttcctga agagcgacgg tttcgcgaac cgtaacttta tgcagctgat
     3661 tcacgatgac agcctgacct ttaaagagga catccagaag gcgcaggtga gcggtcaggg
     3721 cgatagcctg cacgaacaca tcgctaacct ggccggtagc ccggccatca agaagggcat
     3781 cctgcaaacc gtgaaggtgg tggacgagct ggtgaaagtg atgggtcgtc acaaaccgga
     3841 aaacatcgtg attgagatgg cccgcgaaaa ccagaccacc cagaaaggcc agaagaacag
     3901 ccgtgagcgc atgaagcgta tcgaggaagg catcaaggaa ctgggcagcc agatcctgaa
     3961 ggagcacccg gtggaaaaca cccagctgca aaacgagaaa ctgtatctgt actacctgca
     4021 aaacggtcgt gacatgtacg tggatcagga gctggacatt aaccgcctga gcgactacga
     4081 tgtggacgcg atcgtgccgc agagcttcct gaaggatgac agcattgaca acaaagtgct
     4141 gacccgtagc gataaaaacc gcggcaagag cgacaacgtg ccgagcgagg aagtggtgaa
     4201 gaaaatgaaa aactactggc gtcagctgct gaacgccaag ctgatcaccc agcgtaaatt
     4261 cgacaacctg accaaggctg agcgcggtgg cctgagcgaa ctggataaag ccggttttat
     4321 taagcgtcag ctggtggaaa cccgccagat caccaagcac gtggcgcaga ttctggacag
     4381 ccgcatgaac accaaatacg acgagaacga taagctgatc cgtgaagtga aagtgattac
     4441 cctgaagagc aaactggtga gcgatttccg taaggacttc cagttttaca aagtgcgcga
     4501 gatcaacaac tatcaccacg cgcacgacgc ttacctgaac gctgtggtgg gcaccgccct
     4561 gatcaagaag tatccgaagc tggagagcga gttcgtgtac ggcgattata aagtgtacga
     4621 cgtgcgtaaa atgatcgcca agagcgagca ggaaattggc aaagcgaccg ctaagtattt
     4681 cttttacagc aacatcatga acttctttaa gaccgagatc accctggcca acggtgaaat
     4741 ccgtaaacgc ccgctgattg aaaccaacgg tgaaaccggc gaaatcgtgt gggataaggg
     4801 ccgtgacttc gcgaccgtgc gcaaagtgct gtccatgccg caggtgaaca ttgtgaagaa
     4861 aaccgaggtg cagaccggtg gctttagcaa ggaaagcatt ctgccgaaac gtaacagcga
     4921 caagctgatc gcccgcaaga aagattggga cccgaagaaa tatggtggct tcgatagccc
     4981 aaccgtggcc tacagcgtgc tggtggtggc taaagtggag aagggtaaaa gcaagaaact
     5041 gaaaagcgtg aaggaactgc tgggcatcac cattatggag cgtagcagct tcgaaaagaa
     5101 cccgatcgac tttctggagg cgaaaggtta caaggaagtg aagaaagatc tgatcatcaa
     5161 gctgccgaag tatagcctgt tcgagctgga aaatggtcgt aaacgtatgc tggccagcgc
     5221 cggcgagctg caaaagggca acgaactggc gctgccgagc aaatacgtga actttctgta
     5281 tctggccagc cactacgaga agctgaaagg tagcccggag gacaacgaac agaagcagct
     5341 gttcgtggag cagcacaaac actacctgga tgagatcatt gaacagatta gcgagttcag
     5401 caagcgtgtg atcctggctg atgccaacct ggacaaagtg ctgagcgcct ataacaaaca
     5461 ccgtgacaag ccgatccgcg agcaggccga aaacatcatt cacctgttca ccctgaccaa
     5521 cctgggcgcg ccggctgcct tcaaatattt tgataccacc attgaccgta agcgttacac
     5581 cagcaccaaa gaggtgctgg atgctaccct gatccaccag agcattaccg gtctgtacga
     5641 aacccgtatc gatctgagcc agctgggtgg cgacaagcgc ccggcggcta ccaagaaagc
     5701 cggtcaggcg aagaaaaaga aagactacaa ggatgacgat gacaagggta gcggcgctac
     5761 caacttcagc ctgctgaagc aggcgggcga cgtggaggaa aatccgggcc cgatggctaa
     5821 accgctgagc caggaggaaa gcaccctgat cgagcgcgcg accgctacca ttaacagcat
     5881 cccgattagc gaagattata gcgtggccag cgccgcgctg agcagcgatg gtcgtatctt
     5941 caccggcgtg aacgtgtatc actttaccgg tggcccgtgc gccgagctgg tggtgctggg
     6001 caccgccgcc gccgccgccg ccggcaacct gacctgcatc gtggcgattg gtaacgaaaa
     6061 tcgtggcatc ctgagcccgt gcggccgttg ccgccaggtg ctgctggacc tgcatccggg
     6121 catcaaggcg attgtgaagg atagcgacgg ccaaccgacc gctgtgggta tccgtgaact
     6181 gctgccatcc ggctatgttt gggagggcta aggtaccgag ctcggatcca ctagtccagt
     6241 gtggtggaat tctgcagata tccagcacag tggcggccgc tcgagtctag agggcccgtt
     6301 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     6361 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     6421 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     6481 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     6541 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     6601 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6661 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6721 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6781 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     6841 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     6901 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     6961 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     7021 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     7081 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     7141 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     7201 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7261 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     7321 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     7381 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     7441 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     7501 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     7561 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     7621 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     7681 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     7741 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     7801 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     7861 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     7921 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     7981 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     8041 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     8101 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     8161 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     8221 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     8281 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     8341 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     8401 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     8461 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     8521 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     8581 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     8641 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     8701 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     8761 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     8821 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     8881 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8941 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     9001 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     9061 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     9121 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     9181 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     9241 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     9301 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     9361 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     9421 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     9481 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     9541 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     9601 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     9661 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     9721 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9781 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9841 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9901 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9961 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    10021 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    10081 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    10141 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    10201 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    10261 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    10321 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    10381 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    10441 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    10501 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    10561 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    10621 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    10681 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
    10741 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
    10801 cagtatctgc tccctgctt
//
