LOCUS       sequence-269050-       10975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of circular permutant of nSpyCas9 lacking uracil DNA 
            glycosylase inhibitor at amino acid position 1029 encoding rat 
            cytosine deaminase, rAPOBEC3 at the N-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10975)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 10975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: S1 1029 CP APOBEC3 no UGI
FEATURES             Location/Qualifiers
     source          1..10975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3106..3153
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             3154..3177
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3187..3243
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(3736..3753)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3742..3966
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4012..4440
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4099..4118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4309..4330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4449..4469)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4454..4783
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4634..4769
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4696..4715
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4850..5644
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4904..4923)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5514..5533
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5818..5939
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5855..5874)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5909..5928
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5988..6004)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5988..6004)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6001..6023)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6012..6028
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6036..6042,6043..6060,6061..6066))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6081..6102
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6219..6236)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6390..6978)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6470..6489)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7149..7940,7941..8009))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7772..7791
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8010..8114)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8189..8208)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8380..8759
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8760..8963
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8914..8934
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9008..9027
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9008..9026
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9074..9760
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             9761..9781
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9797..9817
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aagaaatata gcatcggtct ggcgattggc accaacagcg tgggttgggc tgtgatcacc
       61 gatgagtaca aggtgccgag caagaagttc aaggtgctgg gcaacaccga ccgccacagc
      121 atcaagaaaa acctgattgg tgccctgctg tttgatagcg gtgaaaccgc cgaggccacc
      181 cgtctgaagc gcaccgcccg tcgccgttac acccgtcgta aaaaccgtat ctgctacctg
      241 caagagattt tcagcaacga aatggccaaa gtggacgata gcttctttca ccgcctggag
      301 gagagcttcc tggtggagga agacaagaaa cacgagcgtc acccgatctt tggcaacatt
      361 gtggatgagg tggcttacca cgaaaagtat ccgaccatct accacctgcg taagaaactg
      421 gtggatagca ccgacaaggc cgatctgcgc ctgatctatc tggctctggc ccacatgatc
      481 aagttccgtg gtcactttct gatcgagggc gacctgaacc cggataacag cgatgtggac
      541 aagctgttca tccagctggt gcagacctac aaccagctgt ttgaggaaaa cccgattaac
      601 gccagcggtg tggacgcgaa ggctatcctg agcgcgcgtc tgagcaaaag ccgccgtctg
      661 gagaacctga tcgcgcagct gccgggtgaa aagaaaaacg gtctgtttgg caacctgatt
      721 gctctgagcc tgggcctgac cccgaacttc aaaagcaact ttgacctggc ggaggatgct
      781 aagctgcaac tgagcaagga tacctatgac gatgacctgg acaacctgct ggcgcagatc
      841 ggcgaccagt acgctgatct gttcctggct gccaagaacc tgagcgatgc gatcctgctg
      901 agcgacattc tgcgtgtgaa caccgagatt accaaggccc cgctgagcgc gagcatgatc
      961 aaacgctatg atgaacacca ccaggacctg accctgctga aagctctggt gcgtcagcag
     1021 ctgccggaga agtacaaaga aatcttcttt gaccagagca agaacggtta cgccggctat
     1081 attgatggcg gtgcgagcca ggaggagttc tacaagttta tcaaaccgat tctggagaaa
     1141 atggacggca ccgaggaact gctggtgaag ctgaaccgtg aagatctgct gcgtaaacag
     1201 cgcaccttcg acaacggtag catcccgcac cagattcacc tgggtgagct gcacgccatc
     1261 ctgcgccgtc aggaagattt ctacccgttt ctgaaggaca accgtgagaa gatcgaaaaa
     1321 attctgacct tccgcattcc gtactatgtg ggtccgctgg ctcgtggcaa cagccgcttt
     1381 gcctggatga cccgcaagag cgaggaaacc atcaccccgt ggaacttcga ggaagtggtg
     1441 gacaaaggcg ccagcgcgca gagcttcatt gagcgtatga ccaactttga taagaacctg
     1501 ccgaacgaaa aagtgctgcc gaagcacagc ctgctgtacg agtatttcac cgtgtacaac
     1561 gaactgacca aggtgaaata tgtgaccgag ggtatgcgca agccggcctt cctgagcggc
     1621 gaacagaaga aagcgatcgt ggacctgctg tttaaaacca accgtaaggt gaccgtgaag
     1681 cagctgaaag aggattattt caagaaaatt gaatgctttg acagcgtgga gatcagcggt
     1741 gtggaagatc gttttaacgc gagcctgggc acctaccacg acctgctgaa aatcattaag
     1801 gataaagact tcctggataa cgaggaaaac gaggacatcc tggaagatat tgtgctgacc
     1861 ctgaccctgt ttgaggaccg tgaaatgatc gaggaacgcc tgaagaccta tgcccacctg
     1921 ttcgatgaca aagtgatgaa acagctgaag cgccgtcgct acaccggttg gggccgtctg
     1981 agccgcaaac tgatcaacgg tattcgcgat aaacagagcg gcaagaccat cctggatttt
     2041 ctgaagagcg acggtttcgc gaaccgtaac tttatgcagc tgattcacga tgacagcctg
     2101 accttcaaag aggacatcca gaaggcgcag gtgagcggtc agggcgatag cctgcacgaa
     2161 catattgcta acctggccgg tagcccggcc atcaagaagg gcatcctgca aaccgtgaag
     2221 gtggtggacg agctggtgaa agtgatgggt cgtcacaagc cggaaaacat cgtgattgag
     2281 atggcccgcg aaaaccagac cacccagaaa ggccagaaga acagccgtga gcgcatgaag
     2341 cgtatcgagg aaggcatcaa ggaactgggc agccagatcc tgaaggagca cccggtggaa
     2401 aacacccagc tgcaaaacga gaaactgtac ctgtactacc tgcaaaacgg tcgtgacatg
     2461 tatgtggatc aggaactgga cattaaccgc ctgagcgatt atgacgtgga tgcgatcgtg
     2521 ccgcagagct tcctgaagga tgacagcatt gacaacaaag tgctgacccg tagcgataaa
     2581 aaccgcggca agagcgacaa cgtgccgagc gaggaagtgg tgaagaaaat gaaaaactac
     2641 tggcgtcagc tgctgaacgc caaactgatc acccagcgta agtttgacaa cctgaccaaa
     2701 gctgagcgcg gcggtctgag cgaactggat aaagccggtt tcattaagcg tcagctggtg
     2761 gaaacccgcc agatcaccaa gcacgtggcg cagattctgg acagccgcat gaacaccaag
     2821 tatgacgaga acgataaact gatccgtgaa gtgaaagtga tcaccctgaa gagcaaactg
     2881 gtgagcgatt ttcgtaaaga cttccagttt tacaaggtgc gcgagatcaa caactaccac
     2941 cacgctcacg acgcctatct gaacgcggtg gtgggcaccg ccctgatcaa gaagtacccg
     3001 aagctggaga gcgagttcgt gtacggcgac tacaaggtgt atgatgtgcg taagatgatc
     3061 gccaaaagcg agcaggaaat tggcggcggc gatagcggtg gtagcaagcg tccggcggct
     3121 accaagaaag cgggccaggc taagaaaaag aaagactaca aagatgacga tgacaagggt
     3181 agcggcgcta ccaacttcag cctgctgaag caggcgggcg acgtggagga aaatccgggc
     3241 ccgatggcta aaccgctgag ccaggaggaa agcaccctga tcgagcgcgc taccgccacc
     3301 attaacagca tcccgattag cgaagactat agcgtggcca gcgccgcgct gagcagcgat
     3361 ggtcgtattt tcaccggcgt gaacgtgtac cactttaccg gtggcccgtg cgccgagctg
     3421 gtggtgctgg gcaccgccgc cgccgccgcc gccggcaacc tgacctgcat cgtggcgatt
     3481 ggtaacgaaa atcgtggcat cctgagcccg tgcggccgtt gccgccaggt gctgctggac
     3541 ctgcatccgg gcatcaaggc gattgtgaag gatagcgacg gccaaccgac cgccgtgggt
     3601 attcgtgaac tgctgccatc cggctatgtt tgggagggct aaggatccac tagtccagtg
     3661 tggtggaatt ctgcagatat ccagcacagt ggcggccgct cgagtctaga gggcccgttt
     3721 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     3781 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     3841 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     3901 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     3961 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     4021 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     4081 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     4141 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     4201 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     4261 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     4321 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     4381 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4441 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     4501 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     4561 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     4621 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     4681 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     4741 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     4801 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     4861 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     4921 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     4981 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     5041 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     5101 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     5161 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     5221 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     5281 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     5341 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     5401 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     5461 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     5521 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     5581 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     5641 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     5701 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     5761 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     5821 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     5881 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     5941 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     6001 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     6061 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     6121 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     6181 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     6241 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     6301 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     6361 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     6421 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     6481 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     6541 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     6601 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     6661 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     6721 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     6781 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     6841 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     6901 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     6961 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7021 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7081 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7141 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7201 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7261 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     7321 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     7381 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     7441 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     7501 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     7561 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     7621 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     7681 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     7741 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     7801 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     7861 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     7921 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     7981 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8041 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     8101 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     8161 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     8221 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
     8281 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
     8341 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
     8401 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     8461 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     8521 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     8581 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     8641 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     8701 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     8761 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     8821 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     8881 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     8941 gggaggtcta tataagcaga gctctctggc taactagaga acccactgct tactggctta
     9001 tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta aacttaagct
     9061 tggtaccgcc accatgagct cagagactgg cccagtggct gtggacccca cattgagacg
     9121 gcggatcgag ccccatgagt ttgaggtatt cttcgatccg agagagctcc gcaaggagac
     9181 ctgcctgctt tacgaaatta attggggggg ccggcactcc atttggcgac atacatcaca
     9241 gaacactaac aagcacgtcg aagtcaactt catcgagaag ttcacgacag aaagatattt
     9301 ctgtccgaac acaaggtgca gcattacctg gtttctcagc tggagcccat gcggcgaatg
     9361 tagtagggcc atcactgaat tcctgtcaag gtatccccac gtcactctgt ttatttacat
     9421 cgcaaggctg taccaccacg ctgacccccg caatcgacaa ggcctgcggg atttgatctc
     9481 ttcaggtgtg actatccaaa ttatgactga gcaggagtca ggatactgct ggagaaactt
     9541 tgtgaattat agcccgagta atgaagccca ctggcctagg tatccccatc tgtgggtacg
     9601 actgtacgtt cttgaactgt actgcatcat actgggcctg cctccttgtc tcaacattct
     9661 gagaaggaag cagccacagc tgacattctt taccatcgct cttcagtctt gtcattacca
     9721 gcgactgccc ccacacattc tctgggccac cgggttgaaa ccgaagaaaa agcgtaaagt
     9781 gggtagcagc ggcagcccga aaaagaaacg caaagtgggt agcgacgctc tggatgactt
     9841 cgacctggat atgctgggca gcgacgccct ggatgacttt ggcggtggca gcataggcaa
     9901 ggcaaccgct aagtattttt tttacagcaa catcatgaac ttctttaaga ccgagattac
     9961 cctggccaac ggtgaaatcc gtaaacgccc gctgattgaa accaacggtg aaaccggcga
    10021 aatcgtgtgg gacaagggcc gtgattttgc gaccgtgcgc aaagtgctgt ccatgccgca
    10081 ggtgaacatt gtgaagaaaa ccgaggtgca gaccggcggt ttcagcaagg aaagcattct
    10141 gccgaaacgt aacagcgaca agctgatcgc ccgcaagaaa gattgggacc cgaagaaata
    10201 cggcggtttc gatagcccga ccgtggctta tagcgtgctg gtggtggcca aagtggagaa
    10261 gggtaaaagc aagaaactga agagcgtgaa agaactgctg ggcatcacca ttatggagcg
    10321 tagcagcttc gaaaagaacc cgatcgactt tctggaggcg aagggttaca aggaagtgaa
    10381 gaaagatctg atcattaagc tgccgaaata cagcctgttt gagctggaaa atggtcgtaa
    10441 acgtatgctg gccagcgccg gcgagctgca aaagggcaac gaactggcgc tgccgagcaa
    10501 atatgtgaac ttcctgtacc tggccagcca ctatgagaag ctgaaaggta gcccggagga
    10561 caacgaacag aagcagctgt ttgtggagca gcacaaacac tatctggatg agatcattga
    10621 acagatcagc gagttcagca agcgtgtgat tctggcggac gctaacctgg ataaagtgct
    10681 gagcgcctac aacaaacacc gtgacaagcc gatccgcgag caggcggaaa acatcattca
    10741 cctgttcacc ctgaccaacc tgggtgcgcc ggccgcgttc aagtactttg acaccaccat
    10801 tgatcgtaaa cgctatacca gcaccaagga ggtgctggat gctaccctga tccaccagag
    10861 cattaccggc ctgtacgaaa cccgtattga tctgagccag ctgggcggtg acggcggtag
    10921 cggcggtagc ggcggtagcg gcggtagcgg cggtagcggc ggtagcggcg gtgac
//
