LOCUS       sequence-269051-       13124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  overexpression in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13124)
  AUTHORS   Yang LZ, Wang Y, Li SQ, Yao RW, Luan PF, Wu H, Carmichael GG, Chen 
            LL
  TITLE     Dynamic Imaging of RNA in Living Cells by CRISPR-Cas13 Systems.
  JOURNAL   Mol Cell. 2019 Dec 19;76(6):981-997.e7. doi: 
            10.1016/j.molcel.2019.10.024. Epub 2019 Nov 19.
  PUBMED    31757757
REFERENCE   2  (bases 1 to 13124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2019.10.024"; journalName: "Mol Cell"; date: 
            "2019-12-19- 19"; volume: "76"; issue: "6"; pages: "981-997.e7"
COMMENT     Sequence Label: pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-3x...
FEATURES             Location/Qualifiers
     source          1..13124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3431..3451
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      7010..7019
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      7742..7751
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8237..8302
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    8309..8876
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8470..8487)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8697..8716
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8896..9495
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8896..8915)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9392..9412
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9514..10102
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9567..9587)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9985..9996)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10177..10410
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10529..10547)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10615..10719
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10720..10788,10789..11580)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10938..10957)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11751..12339
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12240..12259
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12493..12510
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12750..12771
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12786..12791,12792..12809,12810..12816)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12824..12840
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12829..12851
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12848..12864
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12848..12864
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12914..12933
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc accggtctgc
     3421 agctagcatg cccaaaaaga agaggaaagt gaacatcccc gctctggtgg aaaaccagaa
     3481 gaagtacttt ggcacctaca gcgtgatggc catgctgaac gctcagaccg tgctggacca
     3541 catccagaag gtggccgata ttgagggcga gcagaacgag aacaacgaga atctgtggtt
     3601 tcaccccgtg atgagccacc tgtacaacgc caagaacggc tacgacaagc agcccgagaa
     3661 aaccatgttc atcatcgagc ggctgcagag ctacttccca ttcctgaaga tcatggccga
     3721 gaaccagaga gagtacagca acggcaagta caagcagaac cgcgtggaag tgaacagcaa
     3781 cgacatcttc gaggtgctga agcgcgcctt cggcgtgctg aagatgtaca gggacctgac
     3841 caacgcctac aagacctacg aggaaaagct gaacgacggc tgcgagttcc tgaccagcac
     3901 agagcaacct ctgagcggca tgatcaacaa ctactacaca gtggccctgc ggaacatgaa
     3961 cgagagatac ggctacaaga cagaggacct ggccttcatc caggacaagc ggttcaagtt
     4021 cgtgaaggac gcctacggca agaaaaagtc ccaagtgaat accggattct tcctgagcct
     4081 gcaggactac aacggcgaca cacagaagaa gctgcacctg agcggagtgg gaatcgccct
     4141 gctgatctgc ctgttcctgg acaagcagta catcaacatc tttctgagca ggctgcccat
     4201 cttctccagc tacaatgccc agagcgagga acggcggatc atcatcagat ccttcggcat
     4261 caacagcatc aagctgccca aggaccggat ccacagcgag aagtccaaca agagcgtggc
     4321 catggatatg ctcaacgaag tgaagcggtg ccccgacgag ctgttcacaa cactgtctgc
     4381 cgagaagcag tcccggttca gaatcatcag cgacgaccac aatgaagtgc tgatgaagcg
     4441 gagcagcgac agattcgtgc ctctgctgct gcagtatatc gattacggca agctgttcga
     4501 ccacatcagg ttccacgtga acatgggcaa gctgagatac ctgctgaagg ccgacaagac
     4561 ctgcatcgac ggccagacca gagtcagagt gatcgagcag cccctgaacg gcttcggcag
     4621 actggaagag gccgagacaa tgcggaagca agagaacggc accttcggca acagcggcat
     4681 ccggatcaga gacttcgaga acatgaagcg ggacgacgcc aatcctgcca actatcccta
     4741 catcgtggac acctacacac actacatcct ggaaaacaac aaggtcgaga tgtttatcaa
     4801 cgacaaagag gacagcgccc cactgctgcc cgtgatcgag gatgatagat acgtggtcaa
     4861 gacaatcccc agctgccgga tgagcaccct ggaaattcca gccatggcct tccacatgtt
     4921 tctgttcggc agcaagaaaa ccgagaagct gatcgtggac gtgcacaacc ggtacaagag
     4981 actgttccag gccatgcaga aagaagaagt gaccgccgag aatatcgcca gcttcggaat
     5041 cgccgagagc gacctgcctc agaagatcct ggatctgatc agcggcaatg cccacggcaa
     5101 ggatgtggac gccttcatca gactgaccgt ggacgacatg ctgaccgaca ccgagcggag
     5161 aatcaagaga ttcaaggacg accggaagtc cattcggagc gccgacaaca agatgggaaa
     5221 gagaggcttc aagcagatct ccacaggcaa gctggccgac ttcctggcca aggacatcgt
     5281 gctgtttcag cccagcgtga acgatggcga gaacaagatc accggcctga actaccggat
     5341 catgcagagc gccattgccg tgtacgatag cggcgacgat tacgaggcca agcagcagtt
     5401 caagctgatg ttcgagaagg cccggctgat cggcaagggc acaacagagc ctcatccatt
     5461 tctgtacaag gtgttcgccc gcagcatccc cgccaatgcc gtcgagttct acgagcgcta
     5521 cctgatcgag cggaagttct acctgaccgg cctgtccaac gagatcaaga aaggcaacag
     5581 agtggatgtg cccttcatcc ggcgggacca gaacaagtgg aaaacacccg ccatgaagac
     5641 cctgggcaga atctacagcg aggatctgcc cgtggaactg cccagacaga tgttcgacaa
     5701 tgagatcaag tcccacctga agtccctgcc acagatggaa ggcatcgact tcaacaatgc
     5761 caacgtgacc tatctgatcg ccgagtacat gaagagagtg ctggacgacg acttccagac
     5821 cttctaccag tggaaccgca actaccggta catggacatg cttaagggcg agtacgacag
     5881 aaagggctcc ctgcagcact gcttcaccag cgtggaagag agagaaggcc tctggaaaga
     5941 gcgggcctcc agaacagagc ggtacagaaa gcaggccagc aacaagatcc gcagcaaccg
     6001 gcagatgaga aacgccagca gcgaagagat cgagacaatc ctggataagc ggctgagcaa
     6061 cagccggaac gagtaccaga aaagcgagaa agtgatccgg cgctacagag tgcaggatgc
     6121 cctgctgttt ctgctggcca aaaagaccct gaccgaactg gccgatttcg acggcgagag
     6181 gttcaaactg aaagaaatca tgcccgacgc cgagaaggga atcctgagcg agatcatgcc
     6241 catgagcttc accttcgaga aaggcggcaa gaagtacacc atcaccagcg agggcatgaa
     6301 gctgaagaac tacggcgact tctttgtgct ggctagcgac aagaggatcg gcaacctgct
     6361 ggaactcgtg ggcagcgaca tcgtgtccaa agaggatatc atggaagagt tcaacaaata
     6421 cgaccagtgc aggcccgaga tcagctccat cgtgttcaac ctggaaaagt gggccttcga
     6481 cacatacccc gagctgtctg ccagagtgga ccgggaagag aaggtggact tcaagagcat
     6541 cctgaaaatc ctgctgaaca acaagaacat caacaaagag cagagcgaca tcctgcggaa
     6601 gatccggaac gccttcgatg ccaacaatta ccccgacaaa ggcgtggtgg aaatcaaggc
     6661 cctgcctgag atcgccatga gcatcaagaa ggcctttggg gagtacgcca tcatgaagga
     6721 gggagctcta gaggggacta gtggaggtgg atccatggtg tccaagggcg aagaggacaa
     6781 catggccagc ctgcctgcca cccacgagct gcacatcttc ggcagcatca acggcgtgga
     6841 cttcgacatg gtgggacagg gcaccggcaa ccccaacgac ggctacgagg aactgaacct
     6901 gaagtccaca aagggcgacc tgcagttcag cccctggatt ctggtgcccc acatcggcta
     6961 cggcttccac cagtacctgc cctaccccga cggcatgagc ccttttcagg ccgccatggt
     7021 ggatggcagc ggctaccagg tgcaccggac catgcagttt gaggacggcg ccagcctgac
     7081 cgtgaactac cggtacacat acgagggcag ccacatcaag ggcgaggccc aagtgaaggg
     7141 cacaggcttt ccagccgacg gccccgtgat gaccaatagc ctgacagccg ccgactggtg
     7201 cagaagcaag aaaacctacc ccaatgacaa gaccatcatc agcaccttca agtggtccta
     7261 caccaccggc aatggcaagc ggtacagaag caccgcccgg accacctaca ccttcgccaa
     7321 acccatggcc gccaactacc tgaagaacca gcctatgtac gtgttccgca agaccgagct
     7381 gaagcactcc aagacagaac tgaacttcaa agagtggcag aaagccttca ccgacgtgat
     7441 gggcatggac gagctgtaca agtcgaattc tgcagtcgac ggtaccatgg tgtccaaggg
     7501 cgaagaggac aacatggcca gcctgcctgc cacccacgag ctgcacatct tcggcagcat
     7561 caacggcgtg gacttcgaca tggtgggaca gggcaccggc aaccccaacg acggctacga
     7621 ggaactgaac ctgaagtcca caaagggcga cctgcagttc agcccctgga ttctggtgcc
     7681 ccacatcggc tacggcttcc accagtacct gccctacccc gacggcatga gcccttttca
     7741 ggccgccatg gtggatggca gcggctacca ggtgcaccgg accatgcagt ttgaggacgg
     7801 cgccagcctg accgtgaact accggtacac atacgagggc agccacatca agggcgaggc
     7861 ccaagtgaag ggcacaggct ttccagccga cggccccgtg atgaccaata gcctgacagc
     7921 cgccgactgg tgcagaagca agaaaaccta ccccaatgac aagaccatca tcagcacctt
     7981 caagtggtcc tacaccaccg gcaatggcaa gcggtacaga agcaccgccc ggaccaccta
     8041 caccttcgcc aaacccatgg ccgccaacta cctgaagaac cagcctatgt acgtgttccg
     8101 caagaccgag ctgaagcact ccaagacaga actgaacttc aaagagtggc agaaagcctt
     8161 caccgacgtg atgggcatgg acgagctgta caagtccgga ctcagatctt gcctagagca
     8221 acgcgtgcag ctaagcgact acaaagacca tgacggtgat tataaagatc atgacatcga
     8281 ttacaaggat gacgatgaca agtaaggatc cctccccccc ccctaacgtt actggccgaa
     8341 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
     8401 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
     8461 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
     8521 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
     8581 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
     8641 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
     8701 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
     8761 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
     8821 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataaggat
     8881 ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     8941 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     9001 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     9061 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     9121 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     9181 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     9241 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     9301 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     9361 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     9421 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     9481 aagcccggtg cctgacgccc atcgatagat cctaatcaac ctctggatta caaaatttgt
     9541 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     9601 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     9661 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     9721 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     9781 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     9841 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     9901 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     9961 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
    10021 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
    10081 tccctttggg ccgcctcccc gcctgagatc ctttaagacc aatgacttac aaggcagctg
    10141 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
    10201 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
    10261 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
    10321 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
    10381 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
    10441 atttataact tgcaaagaaa tgaatatcag agagtgagag gcccgggtta attaaggaaa
    10501 gggctagatc attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
    10561 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
    10621 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
    10681 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
    10741 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
    10801 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
    10861 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
    10921 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
    10981 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
    11041 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
    11101 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
    11161 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
    11221 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
    11281 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
    11341 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
    11401 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
    11461 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
    11521 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
    11581 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
    11641 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
    11701 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    11761 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    11821 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    11881 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
    11941 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    12001 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    12061 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    12121 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    12181 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    12241 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    12301 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    12361 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
    12421 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
    12481 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
    12541 cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga ctaatttttt
    12601 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
    12661 gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag gtttcccgac
    12721 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
    12781 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
    12841 tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc atttttttca
    12901 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
    12961 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
    13021 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
    13081 taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
