LOCUS       sequence-269052-       11326 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of circular permutant of nSpyCas9 at amino acid position 
            1029 encoding ABEMax (wildtype Tada monomer coupled with evolved 
            Tada monomer) at the C-terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11326)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 11326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: S1 1029 CP ABEMax strategy 3
FEATURES             Location/Qualifiers
     source          1..11326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3083..3103
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3119..3139
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4328..4375
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4385..4441
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(4901..4918)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4907..5131
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5177..5605
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5264..5283)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5474..5495
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5614..5634)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5619..5948
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5799..5934
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5861..5880
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6015..6809
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6069..6088)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6679..6698
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6983..7104
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7020..7039)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7074..7093
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7153..7169)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7153..7169)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7166..7188)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7177..7193
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7201..7207,7208..7225,7226..7231))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7246..7267
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7384..7401)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7555..8143)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7635..7654)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8314..9105,9106..9174))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8937..8956
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9175..9279)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9354..9373)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9545..9924
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9925..10128
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10079..10099
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     10173..10192
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10173..10191
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gaaatatagc atcggtctgg cgattggcac caacagcgtg ggttgggctg tgatcaccga
       61 tgagtacaag gtgccgagca agaagttcaa ggtgctgggc aacaccgacc gccacagcat
      121 caagaaaaac ctgattggtg ccctgctgtt tgatagcggt gaaaccgccg aggccacccg
      181 tctgaagcgc accgcccgtc gccgttacac ccgtcgtaaa aaccgtatct gctacctgca
      241 agagattttc agcaacgaaa tggccaaagt ggacgatagc ttctttcacc gcctggagga
      301 gagcttcctg gtggaggaag acaagaaaca cgagcgtcac ccgatctttg gcaacattgt
      361 ggatgaggtg gcttaccacg aaaagtatcc gaccatctac cacctgcgta agaaactggt
      421 ggatagcacc gacaaggccg atctgcgcct gatctatctg gctctggccc acatgatcaa
      481 gttccgtggt cactttctga tcgagggcga cctgaacccg gataacagcg atgtggacaa
      541 gctgttcatc cagctggtgc agacctacaa ccagctgttt gaggaaaacc cgattaacgc
      601 cagcggtgtg gacgcgaagg ctatcctgag cgcgcgtctg agcaaaagcc gccgtctgga
      661 gaacctgatc gcgcagctgc cgggtgaaaa gaaaaacggt ctgtttggca acctgattgc
      721 tctgagcctg ggcctgaccc cgaacttcaa aagcaacttt gacctggcgg aggatgctaa
      781 gctgcaactg agcaaggata cctatgacga tgacctggac aacctgctgg cgcagatcgg
      841 cgaccagtac gctgatctgt tcctggctgc caagaacctg agcgatgcga tcctgctgag
      901 cgacattctg cgtgtgaaca ccgagattac caaggccccg ctgagcgcga gcatgatcaa
      961 acgctatgat gaacaccacc aggacctgac cctgctgaaa gctctggtgc gtcagcagct
     1021 gccggagaag tacaaagaaa tcttctttga ccagagcaag aacggttacg ccggctatat
     1081 tgatggcggt gcgagccagg aggagttcta caagtttatc aaaccgattc tggagaaaat
     1141 ggacggcacc gaggaactgc tggtgaagct gaaccgtgaa gatctgctgc gtaaacagcg
     1201 caccttcgac aacggtagca tcccgcacca gattcacctg ggtgagctgc acgccatcct
     1261 gcgccgtcag gaagatttct acccgtttct gaaggacaac cgtgagaaga tcgaaaaaat
     1321 tctgaccttc cgcattccgt actatgtggg tccgctggct cgtggcaaca gccgctttgc
     1381 ctggatgacc cgcaagagcg aggaaaccat caccccgtgg aacttcgagg aagtggtgga
     1441 caaaggcgcc agcgcgcaga gcttcattga gcgtatgacc aactttgata agaacctgcc
     1501 gaacgaaaaa gtgctgccga agcacagcct gctgtacgag tatttcaccg tgtacaacga
     1561 actgaccaag gtgaaatatg tgaccgaggg tatgcgcaag ccggccttcc tgagcggcga
     1621 acagaagaaa gcgatcgtgg acctgctgtt taaaaccaac cgtaaggtga ccgtgaagca
     1681 gctgaaagag gattatttca agaaaattga atgctttgac agcgtggaga tcagcggtgt
     1741 ggaagatcgt tttaacgcga gcctgggcac ctaccacgac ctgctgaaaa tcattaagga
     1801 taaagacttc ctggataacg aggaaaacga ggacatcctg gaagatattg tgctgaccct
     1861 gaccctgttt gaggaccgtg aaatgatcga ggaacgcctg aagacctatg cccacctgtt
     1921 cgatgacaaa gtgatgaaac agctgaagcg ccgtcgctac accggttggg gccgtctgag
     1981 ccgcaaactg atcaacggta ttcgcgataa acagagcggc aagaccatcc tggattttct
     2041 gaagagcgac ggtttcgcga accgtaactt tatgcagctg attcacgatg acagcctgac
     2101 cttcaaagag gacatccaga aggcgcaggt gagcggtcag ggcgatagcc tgcacgaaca
     2161 tattgctaac ctggccggta gcccggccat caagaagggc atcctgcaaa ccgtgaaggt
     2221 ggtggacgag ctggtgaaag tgatgggtcg tcacaagccg gaaaacatcg tgattgagat
     2281 ggcccgcgaa aaccagacca cccagaaagg ccagaagaac agccgtgagc gcatgaagcg
     2341 tatcgaggaa ggcatcaagg aactgggcag ccagatcctg aaggagcacc cggtggaaaa
     2401 cacccagctg caaaacgaga aactgtacct gtactacctg caaaacggtc gtgacatgta
     2461 tgtggatcag gaactggaca ttaaccgcct gagcgattat gacgtggatg cgatcgtgcc
     2521 gcagagcttc ctgaaggatg acagcattga caacaaagtg ctgacccgta gcgataaaaa
     2581 ccgcggcaag agcgacaacg tgccgagcga ggaagtggtg aagaaaatga aaaactactg
     2641 gcgtcagctg ctgaacgcca aactgatcac ccagcgtaag tttgacaacc tgaccaaagc
     2701 tgagcgcggc ggtctgagcg aactggataa agccggtttc attaagcgtc agctggtgga
     2761 aacccgccag atcaccaagc acgtggcgca gattctggac agccgcatga acaccaagta
     2821 tgacgagaac gataaactga tccgtgaagt gaaagtgatc accctgaaga gcaaactggt
     2881 gagcgatttt cgtaaagact tccagtttta caaggtgcgc gagatcaaca actaccacca
     2941 cgctcacgac gcctatctga acgcggtggt gggcaccgcc ctgatcaaga agtacccgaa
     3001 gctggagagc gagttcgtgt acggcgacta caaggtgtat gatgtgcgta agatgatcgc
     3061 caaaagcgag caggaaattg gcccgaagaa aaagcgtaaa gtgggtagca gcggcagccc
     3121 gaaaaagaaa cgcaaagtgg gtagcgacgc tctggatgac ttcgacctgg atatgctggg
     3181 cagcgacgcc ctggatgact ttggcggtgg cagctctgaa gtcgagttta gccacgagta
     3241 ttggatgagg cacgcactga ccctggcaaa gcgagcatgg gatgaaagag aagtccccgt
     3301 gggcgccgtg ctggtgcaca acaatagagt gatcggagag ggatggaaca ggccaatcgg
     3361 ccgccacgac cctaccgcac acgcagagat catggcactg aggcagggag gcctggtcat
     3421 gcagaattac cgcctgatcg atgccaccct gtatgtgaca ctggagccat gcgtgatgtg
     3481 cgcaggagca atgatccaca gcaggatcgg aagagtggtg ttcggagcac gggacgccaa
     3541 gaccggcgca gcaggctccc tgatggatgt gctgcaccac cccggcatga accaccgggt
     3601 ggagatcaca gagggaatcc tggcagacga gtgcgccgcc ctgctgagcg atttctttag
     3661 aatgcggaga caggagatca aggcccagaa gaaggcacag agctccaccg actctggagg
     3721 atctagcgga ggatcctctg gaagcgagac accaggcaca agcgagtccg ccacaccaga
     3781 gagctccggc ggctcctccg gaggatcctc tgaggtggag ttttcccacg agtactggat
     3841 gagacatgcc ctgaccctgg ccaagagggc acgcgatgag agggaggtgc ctgtgggagc
     3901 cgtgctggtg ctgaacaata gagtgatcgg cgagggctgg aacagagcca tcggcctgca
     3961 cgacccaaca gcccatgccg aaattatggc cctgagacag ggcggcctgg tcatgcagaa
     4021 ctacagactg attgacgcca ccctgtacgt gacattcgag ccttgcgtga tgtgcgccgg
     4081 cgccatgatc cactctagga tcggccgcgt ggtgtttggc gtgaggaacg caaaaaccgg
     4141 cgccgcaggc tccctgatgg acgtgctgca ctaccccggc atgaatcacc gcgtcgaaat
     4201 taccgaggga atcctggcag atgaatgtgc cgccctgctg tgctatttct ttcggatgcc
     4261 tagacaggtg ttcaatgctc agaagaaggc ccagagctcc accgacggcg gcgatagcgg
     4321 tggtagcaag cgtccggcgg ctaccaagaa agcgggccag gctaagaaaa agaaaggtag
     4381 cggcgctacc aacttcagcc tgctgaagca ggcgggcgac gtggaggaaa atccgggccc
     4441 gatggctaaa ccgctgagcc aggaggaaag caccctgatc gagcgcgcta ccgccaccat
     4501 taacagcatc ccgattagcg aagactatag cgtggccagc gccgcgctga gcagcgatgg
     4561 tcgtattttc accggcgtga acgtgtacca ctttaccggt ggcccgtgcg ccgagctggt
     4621 ggtgctgggc accgccgccg ccgccgccgc cggcaacctg acctgcatcg tggcgattgg
     4681 taacgaaaat cgtggcatcc tgagcccgtg cggccgttgc cgccaggtgc tgctggacct
     4741 gcatccgggc atcaaggcga ttgtgaagga tagcgacggc caaccgaccg ccgtgggtat
     4801 tcgtgaactg ctgccatccg gctatgtttg ggagggctaa gatatccagc acagtggcgg
     4861 ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     4921 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     4981 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     5041 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     5101 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     5161 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     5221 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     5281 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     5341 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     5401 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     5461 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     5521 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     5581 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     5641 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     5701 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     5761 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     5821 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     5881 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     5941 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
     6001 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     6061 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     6121 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     6181 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     6241 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     6301 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     6361 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     6421 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     6481 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     6541 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     6601 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     6661 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     6721 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     6781 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     6841 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     6901 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     6961 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     7021 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7081 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     7141 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     7201 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     7261 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     7321 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     7381 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     7441 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     7501 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7561 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7621 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7681 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7741 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     7801 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     7861 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     7921 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     7981 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     8041 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     8101 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     8161 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     8221 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     8281 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     8341 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     8401 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     8461 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     8521 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     8581 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     8641 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     8701 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     8761 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     8821 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     8881 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     8941 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     9001 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     9061 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     9121 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     9181 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     9241 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     9301 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
     9361 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
     9421 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
     9481 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
     9541 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     9601 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     9661 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     9721 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     9781 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     9841 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     9901 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     9961 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
    10021 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
    10081 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact
    10141 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa
    10201 gctggctagc gtttaaactt aagcttggta ccgccaccat gataggcaag gcaaccgcta
    10261 agtatttttt ttacagcaac atcatgaact tctttaagac cgagattacc ctggccaacg
    10321 gtgaaatccg taaacgcccg ctgattgaaa ccaacggtga aaccggcgaa atcgtgtggg
    10381 acaagggccg tgattttgcg accgtgcgca aagtgctgtc catgccgcag gtgaacattg
    10441 tgaagaaaac cgaggtgcag accggcggtt tcagcaagga aagcattctg ccgaaacgta
    10501 acagcgacaa gctgatcgcc cgcaagaaag attgggaccc gaagaaatac ggcggtttcg
    10561 atagcccgac cgtggcttat agcgtgctgg tggtggccaa agtggagaag ggtaaaagca
    10621 agaaactgaa gagcgtgaaa gaactgctgg gcatcaccat tatggagcgt agcagcttcg
    10681 aaaagaaccc gatcgacttt ctggaggcga agggttacaa ggaagtgaag aaagatctga
    10741 tcattaagct gccgaaatac agcctgtttg agctggaaaa tggtcgtaaa cgtatgctgg
    10801 ccagcgccgg cgagctgcaa aagggcaacg aactggcgct gccgagcaaa tatgtgaact
    10861 tcctgtacct ggccagccac tatgagaagc tgaaaggtag cccggaggac aacgaacaga
    10921 agcagctgtt tgtggagcag cacaaacact atctggatga gatcattgaa cagatcagcg
    10981 agttcagcaa gcgtgtgatt ctggcggacg ctaacctgga taaagtgctg agcgcctaca
    11041 acaaacaccg tgacaagccg atccgcgagc aggcggaaaa catcattcac ctgttcaccc
    11101 tgaccaacct gggtgcgccg gccgcgttca agtactttga caccaccatt gatcgtaaac
    11161 gctataccag caccaaggag gtgctggatg ctaccctgat ccaccagagc attaccggcc
    11221 tgtacgaaac ccgtattgat ctgagccagc tgggcggtga cggcggtagc ggcggtagcg
    11281 gcggtagcgg cggtagcggc ggtagcggcg gtagcggcgg tgacaa
//
