LOCUS       sequence-269053-       10783 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of intradomain insertion of miniABEMax (evolved Tada 
            monomer with V82G) at position 1058 of nSpyCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10783)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 10783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: S2 1058 V82G ABEMax
FEATURES             Location/Qualifiers
     source          1..10783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        172..551
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        552..755
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     706..726
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     800..819
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        800..818
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      854..863
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4034..4054
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4070..4090
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5669..5716
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5717..5740
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5750..5806
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(6311..6328)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6317..6541
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6587..7015
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6674..6693)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6884..6905
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7024..7044)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7029..7358
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7209..7344
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7271..7290
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7425..8219
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7479..7498)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8089..8108
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8393..8514
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8430..8449)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8484..8503
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8563..8579)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8563..8579)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8576..8598)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8587..8603
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8611..8617,8618..8635,8636..8641))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8656..8677
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8794..8811)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8965..9553)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9045..9064)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9724..10515,10516..10584))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10347..10366
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10585..10689)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10764..10783)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
       61 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
      121 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
      181 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
      241 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      301 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      361 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      421 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      481 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      541 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      601 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      661 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      721 ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg
      781 cttactggct tatcgaaatt aatacgactc actataggga gacccaagct ggctagcgtt
      841 taaacttaag cttgccacca tggataaaaa atatagcatt ggtctggcga ttggcaccaa
      901 ctccgtgggc tgggctgtga ttaccgatga atacaaggtt ccgagcaaga agtttaaggt
      961 gctgggtaac accgaccgtc acagcatcaa gaagaacctg attggcgctc tgctgtttga
     1021 cagcggcgag accgcggaag ccacccgcct gaagcgcacc gcgcgtcgcc gttatacccg
     1081 tcgtaagaac cgcatttgct atctgcaaga aatcttcagc aacgagatgg cgaaagtgga
     1141 tgacagcttt ttccaccgcc tggaagagag ctttctggtg gaggaagata agaagcacga
     1201 gcgtcacccg atcttcggta acattgtgga cgaggtggcc tatcacgaaa agtatccgac
     1261 catctaccac ctgcgtaaaa aactggtgga tagcaccgac aaagctgatc tgcgcctgat
     1321 ctatctggct ctggcgcaca tgatcaaatt ccgcggccac ttcctgattg agggtgacct
     1381 gaacccggac aacagcgatg tggataaact gtttattcag ctggtgcaga cctataacca
     1441 gctgtttgaa gagaacccga tcaacgcgag cggcgtggac gcgaaagcta ttctgagcgc
     1501 gcgtctgagc aagagccgtc gtctggaaaa cctgatcgcg cagctgccgg gcgagaagaa
     1561 aaacggtctg tttggcaacc tgattgccct gagcctgggt ctgaccccga actttaagag
     1621 caacttcgac ctggcggagg acgcgaaact gcaactgagc aaagatacct atgacgacga
     1681 cctggataac ctgctggcgc agatcggcga tcagtatgct gacctgtttc tggcggccaa
     1741 aaacctgagc gacgcgattc tgctgagcga catcctgcgt gtgaacaccg agattaccaa
     1801 agcgccgctg agcgccagca tgattaagcg ttacgacgaa caccaccagg acctgaccct
     1861 gctgaaagcc ctggtgcgcc agcagctgcc ggagaagtac aaagaaatct ttttcgatca
     1921 gagcaaaaac ggttatgccg gttatattga cggtggcgcg agccaggagg agttttataa
     1981 gttcattaag ccgattctgg aaaagatgga cggcaccgag gagctgctgg tgaagctgaa
     2041 ccgcgaagac ctgctgcgca aacagcgtac ctttgataac ggtagcattc cgcaccagat
     2101 tcacctgggc gaactgcacg cgattctgcg tcgccaggag gacttctatc cgttcctgaa
     2161 ggataaccgt gagaaaattg agaagattct gaccttccgt atcccgtact atgtgggccc
     2221 gctggctcgt ggtaacagcc gtttcgcttg gatgacccgt aaaagcgagg agaccattac
     2281 cccgtggaac ttcgaggaag tggtggacaa aggtgccagc gcgcagagct ttatcgagcg
     2341 catgaccaac ttcgacaaaa acctgccgaa cgaaaaagtg ctgccgaaac acagcctgct
     2401 gtatgaatac ttcaccgtgt ataacgaact gaccaaggtg aagtatgtga ccgaaggtat
     2461 gcgcaagccg gcttttctga gcggcgagca gaagaaggcg attgtggacc tgctgttcaa
     2521 gaccaaccgt aaagtgaccg tgaagcagct gaaggaggac tacttcaaga aaattgaatg
     2581 ctttgacagc gtggagatca gcggcgtgga ggaccgcttc aacgcgagcc tgggcaccta
     2641 ccacgatctg ctgaagatca ttaaggataa agattttctg gacaacgagg aaaacgaaga
     2701 catcctggag gatattgtgc tgaccctgac cctgtttgag gatcgtgaaa tgattgaaga
     2761 acgcctgaag acctatgctc acctgttcga tgataaagtg atgaaacagc tgaagcgtcg
     2821 tcgttatacc ggttggggcc gtctgagccg caaactgatc aacggcattc gtgacaaaca
     2881 gagcggcaag accatcctgg attttctgaa aagcgatggc tttgcgaacc gtaactttat
     2941 gcagctgatt cacgatgata gcctgacctt caaggaggac attcagaagg cgcaggtgag
     3001 cggccagggt gacagcctgc acgagcacat tgcgaacctg gcgggcagcc cggcgattaa
     3061 gaaaggcatt ctgcaaaccg tgaaagtggt ggatgaactg gtgaaagtga tgggccgcca
     3121 caagccggag aacattgtga tcgagatggc ccgtgagaac cagaccaccc agaaaggcca
     3181 gaagaacagc cgcgagcgca tgaagcgtat tgaagagggt atcaaggagc tgggcagcca
     3241 gatcctgaag gaacacccgg tggaaaacac ccagctgcaa aacgagaaac tgtatctgta
     3301 ctacctgcaa aacggccgtg acatgtatgt ggatcaggaa ctggacatca accgcctgag
     3361 cgactacgac gtggatgcga tcgtgccgca gagcttcctg aaagacgaca gcattgataa
     3421 caaggtgctg acccgtagcg acaagaaccg tggcaaaagc gataacgtgc cgagcgagga
     3481 ggtggtgaaa aagatgaaaa actattggcg ccagctgctg aacgcgaagc tgattaccca
     3541 gcgcaagttc gacaacctga ccaaggctga gcgtggtggc ctgagcgagc tggacaaggc
     3601 tggctttatc aaacgccagc tggtggagac ccgccagatc accaagcacg tggcgcagat
     3661 cctggatagc cgtatgaaca ccaaatacga tgagaacgat aagctgatcc gcgaggtgaa
     3721 agtgatcacc ctgaagagca agctggtgag cgattttcgc aaggacttcc agttctacaa
     3781 ggtgcgcgaa atcaacaact accaccacgc gcacgatgcg tatctgaacg cggtggtggg
     3841 caccgctctg atcaagaaat atccgaagct ggagagcgag ttcgtgtacg gtgactataa
     3901 agtgtacgac gtgcgcaaaa tgattgccaa gagcgagcag gagattggta aagctaccgc
     3961 taaatatttc ttttacagca acatcatgaa cttctttaag accgagatca ccctggccaa
     4021 cggtgaaatc cgtccgaaga agaaacgtaa ggtgggtagc agcggtagcc cgaaaaagaa
     4081 gcgcaaagtg ggcagcgacg cgctggacga tttcgatctg gacatgctgg gcagcgatgc
     4141 gctggatgat ttcggcggtg gcagctctga ggtggagttt tcccacgagt actggatgag
     4201 acatgccctg accctggcca agagggcacg cgatgagagg gaggtgcctg tgggagccgt
     4261 gctggtgctg aacaatagag tgatcggcga gggctggaac agagccatcg gcctgcacga
     4321 cccaacagcc catgccgaaa ttatggccct gagacagggc ggcctggtca tgcagaacta
     4381 cagactgatt gacgccaccc tgtacggcac attcgagcct tgcgtgatgt gcgccggcgc
     4441 catgatccac tctaggatcg gccgcgtggt gtttggcgtg aggaacgcaa aaaccggcgc
     4501 cgcaggctcc ctgatggacg tgctgcacta ccccggcatg aatcaccgcg tcgaaattac
     4561 cgagggaatc ctggcagatg aatgtgccgc cctgctgtgc tatttctttc ggatgcctag
     4621 acaggtgttc aatgctcaga agaaggccca gagctccacc gacggcggta gcggcggtag
     4681 cggtggtagc ggcggcagcg gtggtagcgg tggcagcggt ggtcgcaaac gcccgctgat
     4741 cgaaaccaac ggcgaaaccg gtgaaatcgt gtgggataag ggtcgcgatt tcgcgaccgt
     4801 gcgcaaggtg ctgagcatgc cgcaggtgaa catcgtgaag aagaccgaag tgcagaccgg
     4861 cggttttagc aaggaaagca tcctgccgaa gcgcaacagc gataagctga tcgcgcgtaa
     4921 gaaggactgg gacccgaaaa agtatggtgg cttcgacagc ccgaccgtgg cgtacagcgt
     4981 gctggtggtg gccaaggtgg aaaagggcaa aagcaaaaaa ctgaaaagcg tgaaggagct
     5041 gctgggtatt accattatgg aacgtagcag ctttgagaag aacccgatcg acttcctgga
     5101 ggcgaagggt tacaaggaag tgaagaaaga tctgatcatc aaactgccga agtacagcct
     5161 gtttgagctg gaaaacggcc gtaagcgtat gctggcgagc gcgggtgagc tgcaaaaagg
     5221 taacgagctg gcgctgccga gcaagtacgt gaactttctg tacctggcga gccactatga
     5281 aaaactgaaa ggcagcccgg aggacaacga gcagaagcag ctgtttgtgg agcagcacaa
     5341 gcactatctg gacgaaatca tcgaacagat cagcgagttc agcaaacgcg tgattctggc
     5401 cgacgccaac ctggacaaag tgctgagcgc ctacaacaag caccgcgata aaccgattcg
     5461 cgaacaggcc gagaacatca ttcacctgtt taccctgacc aacctgggcg cgccggcggc
     5521 gttcaaatac tttgatacca ccattgatcg caaacgctac accagcacca aggaagtgct
     5581 ggatgcgacc ctgatccacc agagcattac cggtctgtac gaaacccgca tcgatctgag
     5641 ccagctgggc ggcgatagcg gtggtagcaa gcgtccggcg gcgaccaaaa aggccggcca
     5701 ggcgaagaag aagaaggact acaaggatga cgatgacaaa ggcagcggcg ccaccaactt
     5761 cagcctgctg aagcaggccg gtgacgtgga agaaaacccc ggcccgatgg cgaagccgct
     5821 gagccaggag gaaagcaccc tgattgagcg cgccaccgcg accatcaaca gcattccgat
     5881 tagcgaagac tacagcgtgg cgagcgccgc tctgagcagc gatggccgta tcttcaccgg
     5941 cgtgaacgtg taccacttta ccggcggtcc gtgcgcggaa ctggtggtgc tgggcaccgc
     6001 ggcggctgcg gctgcgggta acctgacctg catcgtggcc attggtaacg aaaaccgcgg
     6061 cattctgagc ccgtgcggcc gttgccgcca ggtgctgctg gacctgcacc ccggtatcaa
     6121 agcgattgtg aaagatagcg atggtcaacc gaccgctgtg ggtatccgtg aactgctgcc
     6181 atccggctat gtttgggagg gctaaggtac cgagctcgga tccactagtc cagtgtggtg
     6241 gaattctgca gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgtttaaacc
     6301 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     6361 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     6421 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     6481 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     6541 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     6601 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     6661 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     6721 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     6781 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     6841 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     6901 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     6961 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     7021 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     7081 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     7141 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     7201 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     7261 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     7321 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     7381 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     7441 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     7501 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     7561 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     7621 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     7681 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     7741 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     7801 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     7861 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     7921 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     7981 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     8041 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     8101 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     8161 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     8221 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     8281 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     8341 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     8401 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     8461 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     8521 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     8581 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     8641 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     8701 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     8761 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     8821 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     8881 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     8941 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     9001 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     9061 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     9121 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     9181 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     9241 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     9301 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     9361 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     9421 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     9481 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     9541 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     9601 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     9661 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     9721 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     9781 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     9841 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     9901 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9961 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    10021 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    10081 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    10141 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    10201 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    10261 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    10321 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    10381 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    10441 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    10501 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    10561 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    10621 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    10681 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc
    10741 gatcccctat ggtgcactct cagtacaatc tgctctgatg ccg
//
