LOCUS       sequence-269054-        9787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of nSaCas9 intradomain insertion of single evolved TadA 
            monomer (V82G) at the specified position (E735 ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 9787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: nSaCas9 E735 miniABEMax V82G
FEATURES             Location/Qualifiers
     source          1..9787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        179..558
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        559..762
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     713..733
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     807..826
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        807..825
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3056..3076
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3092..3112
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4715..4762
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4763..4786
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4796..4852
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4853..5251
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(5322..5339)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5328..5552
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5598..6026
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5685..5704)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5895..5916
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6035..6055)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6040..6369
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6220..6355
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6282..6301
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6436..7230
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6490..6509)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7100..7119
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7404..7525
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7441..7460)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7495..7514
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7574..7590)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7574..7590)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7587..7609)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7598..7614
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7622..7628,7629..7646,7647..7652))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7667..7688
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7805..7822)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7976..8564)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8056..8075)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8735..9526,9527..9595))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9358..9377
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9596..9700)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(9775..9787,1..7))
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
       61 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
      121 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
      181 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
      241 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      301 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      361 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      421 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      481 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      541 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      601 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      661 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      721 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa
      781 cccactgctt actggcttat cgaaattaat acgactcact atagggagac ccaagctggc
      841 tagcgccacc atgaagcgaa attacattct ggggctggcc attggcatta catcagtggg
      901 ctatggcatc attgactacg agacaaggga cgtgatcgac gccggcgtga gactgttcaa
      961 ggaggccaac gtggagaaca atgagggccg gagatccaag aggggagcaa ggcgcctgaa
     1021 gcggagaagg cgccacagaa tccagagagt gaagaagctg ctgttcgatt acaacctgct
     1081 gaccgaccac tccgagctgt ctggcatcaa tccttatgag gccagagtga agggcctgtc
     1141 ccagaagctg tctgaggagg agtttagcgc cgccctgctg cacctggcaa agaggagagg
     1201 cgtgcacaac gtgaatgagg tggaggagga caccggcaac gagctgtcca caaaggagca
     1261 gatcagccgc aattccaagg ccctggagga gaagtatgtg gccgagctgc agctggagcg
     1321 gctgaagaag gatggcgagg tgaggggctc catcaatcgc ttcaagacct ctgactacgt
     1381 gaaggaggcc aagcagctgc tgaaggtgca gaaggcctac caccagctgg atcagtcctt
     1441 tatcgataca tatatcgacc tgctggagac aaggcgcaca tactatgagg gaccaggaga
     1501 gggctctccc ttcggctgga aggacatcaa ggagtggtac gagatgctga tgggccactg
     1561 cacctatttt ccagaggagc tgagaagcgt gaagtacgcc tataacgccg atctgtacaa
     1621 cgccctgaat gacctgaaca acctggtcat caccagggat gagaacgaga agctggagta
     1681 ctatgagaag ttccagatca tcgagaacgt gttcaagcag aagaagaagc ctacactgaa
     1741 gcagatcgcc aaggagatcc tggtgaacga ggaggacatc aagggctacc gcgtgacctc
     1801 cacaggcaag ccagagttca ccaatctgaa ggtgtatcac gatatcaagg acatcacagc
     1861 ccggaaggag atcatcgaga acgccgagct gctggatcag atcgccaaga tcctgaccat
     1921 ctatcagagc tccgaggaca tccaggagga gctgaccaac ctgaatagcg agctgacaca
     1981 ggaggagatc gagcagatca gcaatctgaa gggctacacc ggcacacaca acctgagcct
     2041 gaaggccatc aatctgatcc tggatgagct gtggcacaca aacgacaatc agatcgccat
     2101 ctttaaccgg ctgaagctgg tgccaaagaa ggtggacctg tcccagcaga aggagatccc
     2161 aaccacactg gtggacgatt tcatcctgtc tcccgtggtg aagcggagct tcatccagag
     2221 catcaaagtg atcaacgcca tcatcaagaa gtacggcctg cccaatgata tcatcatcga
     2281 gctggccagg gagaagaact ccaaggacgc ccagaagatg atcaatgaga tgcagaagag
     2341 gaaccgccag accaatgagc ggatcgagga gatcatcaga accacaggca aggagaacgc
     2401 caagtacctg atcgagaaga tcaagctgca cgatatgcag gagggcaagt gtctgtattc
     2461 tctggaggcc atccctctgg aggacctgct gaacaatcca ttcaactacg aggtggatca
     2521 catcatcccc cggagcgtga gcttcgacaa ttcttttaac aataaggtgc tggtgaagca
     2581 ggaggagaac agcaagaagg gcaataggac ccctttccag tacctgtcta gctccgattc
     2641 taagatcagc tacgagacat tcaagaagca catcctgaat ctggccaagg gcaagggccg
     2701 catcagcaag accaagaagg agtacctgct ggaggagcgg gacatcaaca gattctccgt
     2761 gcagaaggac ttcatcaacc ggaatctggt ggacaccaga tacgccacac gcggcctgat
     2821 gaatctgctg cggtcttatt tcagagtgaa caatctggat gtgaaggtga agagcatcaa
     2881 cggcggcttc acctcctttc tgcggagaaa gtggaagttt aagaaggagc gcaacaaggg
     2941 ctataagcac cacgccgagg atgccctgat catcgccaat gccgacttca tctttaagga
     3001 gtggaagaag ctggacaagg ccaagaaagt gatggagaac cagatgttcg aggagccgaa
     3061 gaagaaacgt aaggtgggta gcagcggtag cccgaaaaag aagcgcaaag tgggcagcga
     3121 cgcgctggac gatttcgatc tggacatgct gggcagcgat gcgctggatg atttcggcgg
     3181 tggcagctct gaggtggagt tttcccacga gtactggatg agacatgccc tgaccctggc
     3241 caagagggca cgcgatgaga gggaggtgcc tgtgggagcc gtgctggtgc tgaacaatag
     3301 agtgatcggc gagggctgga acagagccat cggcctgcac gacccaacag cccatgccga
     3361 aattatggcc ctgagacagg gcggcctggt catgcagaac tacagactga ttgacgccac
     3421 cctgtacggc acattcgagc cttgcgtgat gtgcgccggc gccatgatcc actctaggat
     3481 cggccgcgtg gtgtttggcg tgaggaacgc aaaaaccggc gccgcaggct ccctgatgga
     3541 cgtgctgcac taccccggca tgaatcaccg cgtcgaaatt accgagggaa tcctggcaga
     3601 tgaatgtgcc gccctgctgt gctatttctt tcggatgcct agacaggtgt tcaatgctca
     3661 gaagaaggcc cagagctcca ccgacggcgg tagcggcggt agcggtggta gcggcggcag
     3721 cggtggtagc ggtggcagcg gtggtgagaa gcaggccgag agcatgcccg agatcgagac
     3781 agagcaggag tacaaggaga ttttcatcac acctcaccag atcaagcaca tcaaggactt
     3841 caaggactac aagtattctc acagggtgga taagaagccc aaccgcgagc tgatcaatga
     3901 caccctgtat agcacacgga aggacgataa gggcaatacc ctgatcgtga acaatctgaa
     3961 cggcctgtac gacaaggata atgacaagct gaagaagctg atcaacaagt ctcccgagaa
     4021 gctgctgatg taccaccacg atcctcagac atatcagaag ctgaagctga tcatggagca
     4081 gtacggcgac gagaagaacc cactgtataa gtactatgag gagacaggca actacctgac
     4141 aaagtatagc aagaaggata atggccccgt gatcaagaag atcaagtact atggcaacaa
     4201 gctgaatgcc cacctggaca tcaccgacga ttaccctaac tctcgcaata aggtggtgaa
     4261 gctgagcctg aagccatacc ggttcgacgt gtacctggac aacggcgtgt ataagtttgt
     4321 gacagtgaag aatctggatg tgatcaagaa ggagaactac tatgaggtga acagcaagtg
     4381 ctacgaggag gccaagaagc tgaagaagat cagcaaccag gccgagttca tcgcctcttt
     4441 ttacaacaat gacctgatca agatcaatgg cgagctgtat agagtgatcg gcgtgaacaa
     4501 tgatctgctg aacagaatcg aagtgaatat gatcgacatc acctacaggg agtatctgga
     4561 gaacatgaat gataagaggc cccctcgcat catcaagacc atcgcctcta agacacagag
     4621 catcaagaag tacagcacag acatcctggg gaacctgtat gaagtcaaga gcaagaaaca
     4681 tcctcagatt atcaagaaag gctctggtgg ttctaagcga cctgccgcca caaagaaggc
     4741 tggacaggct aagaagaaga aagattacaa agacgatgac gataagggat ccggcgcaac
     4801 aaacttctct ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatggccaa
     4861 gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat
     4921 ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt
     4981 cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg
     5041 cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa
     5101 caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg
     5161 gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt
     5221 gctgccctct ggttatgtgt gggagggcta agaattctgc agatatccag cacagtggcg
     5281 gccgctcgag tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     5341 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     5401 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     5461 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     5521 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     5581 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     5641 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     5701 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     5761 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     5821 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     5881 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     5941 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     6001 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     6061 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     6121 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     6181 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     6241 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     6301 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     6361 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca agagacagga
     6421 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     6481 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     6541 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     6601 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     6661 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     6721 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     6781 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     6841 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     6901 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     6961 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     7021 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     7081 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     7141 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     7201 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     7261 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     7321 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     7381 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     7441 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     7501 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     7561 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7621 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7681 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7741 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7801 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7861 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7921 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7981 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     8041 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8101 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8161 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8221 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8281 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8341 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8401 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8461 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8521 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8581 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8641 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8701 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8761 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8821 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8881 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8941 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     9001 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     9061 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     9121 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     9181 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     9241 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     9301 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     9361 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     9421 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     9481 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     9541 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9601 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9661 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9721 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat
     9781 ctgctct
//
