LOCUS       sequence-269055-        9787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of nSaCas9 intradomain insertion of single evolved TadA 
            monomer (V82G) at the specified position (G129 ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 9787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: nSaCas9 G129 miniABEMax V82G
FEATURES             Location/Qualifiers
     source          1..9787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        182..561
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        562..765
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     716..736
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     810..829
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        810..828
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1241..1261
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1277..1297
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4718..4765
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4766..4789
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4799..4855
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4856..5254
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(5325..5342)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5331..5555
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5601..6029
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5688..5707)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5898..5919
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6038..6058)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6043..6372
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6223..6358
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6285..6304
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6439..7233
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6493..6512)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7103..7122
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7407..7528
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7444..7463)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7498..7517
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7577..7593)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7577..7593)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7590..7612)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7601..7617
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7625..7631,7632..7649,7650..7655))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7670..7691
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7808..7825)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7979..8567)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8059..8078)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8738..9529,9530..9598))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9361..9380
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9599..9703)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(9778..9787,1..10))
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
       61 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
      121 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
      181 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
      241 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      301 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      361 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      421 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      481 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      541 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      601 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      661 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      721 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga
      781 gaacccactg cttactggct tatcgaaatt aatacgactc actataggga gacccaagct
      841 ggctagcgcc accatgaagc gaaattacat tctggggctg gccattggca ttacatcagt
      901 gggctatggc atcattgact acgagacaag ggacgtgatc gacgccggcg tgagactgtt
      961 caaggaggcc aacgtggaga acaatgaggg ccggagatcc aagaggggag caaggcgcct
     1021 gaagcggaga aggcgccaca gaatccagag agtgaagaag ctgctgttcg attacaacct
     1081 gctgaccgac cactccgagc tgtctggcat caatccttat gaggccagag tgaagggcct
     1141 gtcccagaag ctgtctgagg aggagtttag cgccgccctg ctgcacctgg caaagaggag
     1201 aggcgtgcac aacgtgaatg aggtggagga ggacaccggc ccgaagaaga aacgtaaggt
     1261 gggtagcagc ggtagcccga aaaagaagcg caaagtgggc agcgacgcgc tggacgattt
     1321 cgatctggac atgctgggca gcgatgcgct ggatgatttc ggcggtggca gctctgaggt
     1381 ggagttttcc cacgagtact ggatgagaca tgccctgacc ctggccaaga gggcacgcga
     1441 tgagagggag gtgcctgtgg gagccgtgct ggtgctgaac aatagagtga tcggcgaggg
     1501 ctggaacaga gccatcggcc tgcacgaccc aacagcccat gccgaaatta tggccctgag
     1561 acagggcggc ctggtcatgc agaactacag actgattgac gccaccctgt acggcacatt
     1621 cgagccttgc gtgatgtgcg ccggcgccat gatccactct aggatcggcc gcgtggtgtt
     1681 tggcgtgagg aacgcaaaaa ccggcgccgc aggctccctg atggacgtgc tgcactaccc
     1741 cggcatgaat caccgcgtcg aaattaccga gggaatcctg gcagatgaat gtgccgccct
     1801 gctgtgctat ttctttcgga tgcctagaca ggtgttcaat gctcagaaga aggcccagag
     1861 ctccaccgac ggcggtagcg gcggtagcgg tggtagcggc ggcagcggtg gtagcggtgg
     1921 cagcggtggt ggcaacgagc tgtccacaaa ggagcagatc agccgcaatt ccaaggccct
     1981 ggaggagaag tatgtggccg agctgcagct ggagcggctg aagaaggatg gcgaggtgag
     2041 gggctccatc aatcgcttca agacctctga ctacgtgaag gaggccaagc agctgctgaa
     2101 ggtgcagaag gcctaccacc agctggatca gtcctttatc gatacatata tcgacctgct
     2161 ggagacaagg cgcacatact atgagggacc aggagagggc tctcccttcg gctggaagga
     2221 catcaaggag tggtacgaga tgctgatggg ccactgcacc tattttccag aggagctgag
     2281 aagcgtgaag tacgcctata acgccgatct gtacaacgcc ctgaatgacc tgaacaacct
     2341 ggtcatcacc agggatgaga acgagaagct ggagtactat gagaagttcc agatcatcga
     2401 gaacgtgttc aagcagaaga agaagcctac actgaagcag atcgccaagg agatcctggt
     2461 gaacgaggag gacatcaagg gctaccgcgt gacctccaca ggcaagccag agttcaccaa
     2521 tctgaaggtg tatcacgata tcaaggacat cacagcccgg aaggagatca tcgagaacgc
     2581 cgagctgctg gatcagatcg ccaagatcct gaccatctat cagagctccg aggacatcca
     2641 ggaggagctg accaacctga atagcgagct gacacaggag gagatcgagc agatcagcaa
     2701 tctgaagggc tacaccggca cacacaacct gagcctgaag gccatcaatc tgatcctgga
     2761 tgagctgtgg cacacaaacg acaatcagat cgccatcttt aaccggctga agctggtgcc
     2821 aaagaaggtg gacctgtccc agcagaagga gatcccaacc acactggtgg acgatttcat
     2881 cctgtctccc gtggtgaagc ggagcttcat ccagagcatc aaagtgatca acgccatcat
     2941 caagaagtac ggcctgccca atgatatcat catcgagctg gccagggaga agaactccaa
     3001 ggacgcccag aagatgatca atgagatgca gaagaggaac cgccagacca atgagcggat
     3061 cgaggagatc atcagaacca caggcaagga gaacgccaag tacctgatcg agaagatcaa
     3121 gctgcacgat atgcaggagg gcaagtgtct gtattctctg gaggccatcc ctctggagga
     3181 cctgctgaac aatccattca actacgaggt ggatcacatc atcccccgga gcgtgagctt
     3241 cgacaattct tttaacaata aggtgctggt gaagcaggag gagaacagca agaagggcaa
     3301 taggacccct ttccagtacc tgtctagctc cgattctaag atcagctacg agacattcaa
     3361 gaagcacatc ctgaatctgg ccaagggcaa gggccgcatc agcaagacca agaaggagta
     3421 cctgctggag gagcgggaca tcaacagatt ctccgtgcag aaggacttca tcaaccggaa
     3481 tctggtggac accagatacg ccacacgcgg cctgatgaat ctgctgcggt cttatttcag
     3541 agtgaacaat ctggatgtga aggtgaagag catcaacggc ggcttcacct cctttctgcg
     3601 gagaaagtgg aagtttaaga aggagcgcaa caagggctat aagcaccacg ccgaggatgc
     3661 cctgatcatc gccaatgccg acttcatctt taaggagtgg aagaagctgg acaaggccaa
     3721 gaaagtgatg gagaaccaga tgttcgagga gaagcaggcc gagagcatgc ccgagatcga
     3781 gacagagcag gagtacaagg agattttcat cacacctcac cagatcaagc acatcaagga
     3841 cttcaaggac tacaagtatt ctcacagggt ggataagaag cccaaccgcg agctgatcaa
     3901 tgacaccctg tatagcacac ggaaggacga taagggcaat accctgatcg tgaacaatct
     3961 gaacggcctg tacgacaagg ataatgacaa gctgaagaag ctgatcaaca agtctcccga
     4021 gaagctgctg atgtaccacc acgatcctca gacatatcag aagctgaagc tgatcatgga
     4081 gcagtacggc gacgagaaga acccactgta taagtactat gaggagacag gcaactacct
     4141 gacaaagtat agcaagaagg ataatggccc cgtgatcaag aagatcaagt actatggcaa
     4201 caagctgaat gcccacctgg acatcaccga cgattaccct aactctcgca ataaggtggt
     4261 gaagctgagc ctgaagccat accggttcga cgtgtacctg gacaacggcg tgtataagtt
     4321 tgtgacagtg aagaatctgg atgtgatcaa gaaggagaac tactatgagg tgaacagcaa
     4381 gtgctacgag gaggccaaga agctgaagaa gatcagcaac caggccgagt tcatcgcctc
     4441 tttttacaac aatgacctga tcaagatcaa tggcgagctg tatagagtga tcggcgtgaa
     4501 caatgatctg ctgaacagaa tcgaagtgaa tatgatcgac atcacctaca gggagtatct
     4561 ggagaacatg aatgataaga ggccccctcg catcatcaag accatcgcct ctaagacaca
     4621 gagcatcaag aagtacagca cagacatcct ggggaacctg tatgaagtca agagcaagaa
     4681 acatcctcag attatcaaga aaggctctgg tggttctaag cgacctgccg ccacaaagaa
     4741 ggctggacag gctaagaaga agaaagatta caaagacgat gacgataagg gatccggcgc
     4801 aacaaacttc tctctgctga aacaagccgg agatgtcgaa gagaatcctg gaccgatggc
     4861 caagcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag
     4921 catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat
     4981 cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct
     5041 gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga
     5101 gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc
     5161 tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga
     5221 attgctgccc tctggttatg tgtgggaggg ctaagaattc tgcagatatc cagcacagtg
     5281 gcggccgctc gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
     5341 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     5401 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     5461 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     5521 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     5581 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     5641 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     5701 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     5761 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     5821 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     5881 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     5941 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     6001 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     6061 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     6121 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     6181 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     6241 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     6301 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     6361 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca
     6421 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     6481 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     6541 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     6601 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     6661 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     6721 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     6781 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     6841 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     6901 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     6961 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     7021 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     7081 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     7141 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     7201 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     7261 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     7321 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     7381 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     7441 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7501 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     7561 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     7621 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     7681 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     7741 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     7801 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     7861 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     7921 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     7981 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8041 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     8101 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     8161 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     8221 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     8281 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     8341 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     8401 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     8461 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     8521 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     8581 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     8641 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     8701 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     8761 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     8821 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     8881 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     8941 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9001 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     9061 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9121 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9181 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     9241 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     9301 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     9361 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     9421 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     9481 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     9541 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     9601 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     9661 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     9721 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac
     9781 aatctgc
//
