LOCUS       sequence-269056-        9787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of nSaCas9 intradomain insertion of single evolved TadA 
            monomer (V82G) at the specified position (S740 ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 9787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: nSaCas9 S740 miniABEMax V82G
FEATURES             Location/Qualifiers
     source          1..9787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        157..536
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        537..740
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     691..711
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     785..804
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        785..803
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3049..3069
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3085..3105
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4693..4740
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4741..4764
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4774..4830
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4831..5229
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(5300..5317)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5306..5530
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5576..6004
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5663..5682)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5873..5894
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6013..6033)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6018..6347
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6198..6333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6260..6279
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6414..7208
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6468..6487)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7078..7097
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7382..7503
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7419..7438)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7473..7492
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7552..7568)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7552..7568)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7565..7587)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7576..7592
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7600..7606,7607..7624,7625..7630))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7645..7666
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7783..7800)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7954..8542)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8034..8053)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8713..9504,9505..9573))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9336..9355
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9574..9678)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9753..9772)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
       61 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
      121 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
      181 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      241 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      301 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      361 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      421 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      481 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      541 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      601 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      661 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      721 aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg
      781 aaattaatac gactcactat agggagaccc aagctggcta gcgccaccat gaagcgaaat
      841 tacattctgg ggctggccat tggcattaca tcagtgggct atggcatcat tgactacgag
      901 acaagggacg tgatcgacgc cggcgtgaga ctgttcaagg aggccaacgt ggagaacaat
      961 gagggccgga gatccaagag gggagcaagg cgcctgaagc ggagaaggcg ccacagaatc
     1021 cagagagtga agaagctgct gttcgattac aacctgctga ccgaccactc cgagctgtct
     1081 ggcatcaatc cttatgaggc cagagtgaag ggcctgtccc agaagctgtc tgaggaggag
     1141 tttagcgccg ccctgctgca cctggcaaag aggagaggcg tgcacaacgt gaatgaggtg
     1201 gaggaggaca ccggcaacga gctgtccaca aaggagcaga tcagccgcaa ttccaaggcc
     1261 ctggaggaga agtatgtggc cgagctgcag ctggagcggc tgaagaagga tggcgaggtg
     1321 aggggctcca tcaatcgctt caagacctct gactacgtga aggaggccaa gcagctgctg
     1381 aaggtgcaga aggcctacca ccagctggat cagtccttta tcgatacata tatcgacctg
     1441 ctggagacaa ggcgcacata ctatgaggga ccaggagagg gctctccctt cggctggaag
     1501 gacatcaagg agtggtacga gatgctgatg ggccactgca cctattttcc agaggagctg
     1561 agaagcgtga agtacgccta taacgccgat ctgtacaacg ccctgaatga cctgaacaac
     1621 ctggtcatca ccagggatga gaacgagaag ctggagtact atgagaagtt ccagatcatc
     1681 gagaacgtgt tcaagcagaa gaagaagcct acactgaagc agatcgccaa ggagatcctg
     1741 gtgaacgagg aggacatcaa gggctaccgc gtgacctcca caggcaagcc agagttcacc
     1801 aatctgaagg tgtatcacga tatcaaggac atcacagccc ggaaggagat catcgagaac
     1861 gccgagctgc tggatcagat cgccaagatc ctgaccatct atcagagctc cgaggacatc
     1921 caggaggagc tgaccaacct gaatagcgag ctgacacagg aggagatcga gcagatcagc
     1981 aatctgaagg gctacaccgg cacacacaac ctgagcctga aggccatcaa tctgatcctg
     2041 gatgagctgt ggcacacaaa cgacaatcag atcgccatct ttaaccggct gaagctggtg
     2101 ccaaagaagg tggacctgtc ccagcagaag gagatcccaa ccacactggt ggacgatttc
     2161 atcctgtctc ccgtggtgaa gcggagcttc atccagagca tcaaagtgat caacgccatc
     2221 atcaagaagt acggcctgcc caatgatatc atcatcgagc tggccaggga gaagaactcc
     2281 aaggacgccc agaagatgat caatgagatg cagaagagga accgccagac caatgagcgg
     2341 atcgaggaga tcatcagaac cacaggcaag gagaacgcca agtacctgat cgagaagatc
     2401 aagctgcacg atatgcagga gggcaagtgt ctgtattctc tggaggccat ccctctggag
     2461 gacctgctga acaatccatt caactacgag gtggatcaca tcatcccccg gagcgtgagc
     2521 ttcgacaatt cttttaacaa taaggtgctg gtgaagcagg aggagaacag caagaagggc
     2581 aataggaccc ctttccagta cctgtctagc tccgattcta agatcagcta cgagacattc
     2641 aagaagcaca tcctgaatct ggccaagggc aagggccgca tcagcaagac caagaaggag
     2701 tacctgctgg aggagcggga catcaacaga ttctccgtgc agaaggactt catcaaccgg
     2761 aatctggtgg acaccagata cgccacacgc ggcctgatga atctgctgcg gtcttatttc
     2821 agagtgaaca atctggatgt gaaggtgaag agcatcaacg gcggcttcac ctcctttctg
     2881 cggagaaagt ggaagtttaa gaaggagcgc aacaagggct ataagcacca cgccgaggat
     2941 gccctgatca tcgccaatgc cgacttcatc tttaaggagt ggaagaagct ggacaaggcc
     3001 aagaaagtga tggagaacca gatgttcgag gagaagcagg ccgagagccc gaagaagaaa
     3061 cgtaaggtgg gtagcagcgg tagcccgaaa aagaagcgca aagtgggcag cgacgcgctg
     3121 gacgatttcg atctggacat gctgggcagc gatgcgctgg atgatttcgg cggtggcagc
     3181 tctgaggtgg agttttccca cgagtactgg atgagacatg ccctgaccct ggccaagagg
     3241 gcacgcgatg agagggaggt gcctgtggga gccgtgctgg tgctgaacaa tagagtgatc
     3301 ggcgagggct ggaacagagc catcggcctg cacgacccaa cagcccatgc cgaaattatg
     3361 gccctgagac agggcggcct ggtcatgcag aactacagac tgattgacgc caccctgtac
     3421 ggcacattcg agccttgcgt gatgtgcgcc ggcgccatga tccactctag gatcggccgc
     3481 gtggtgtttg gcgtgaggaa cgcaaaaacc ggcgccgcag gctccctgat ggacgtgctg
     3541 cactaccccg gcatgaatca ccgcgtcgaa attaccgagg gaatcctggc agatgaatgt
     3601 gccgccctgc tgtgctattt ctttcggatg cctagacagg tgttcaatgc tcagaagaag
     3661 gcccagagct ccaccgacgg cggtagcggc ggtagcggtg gtagcggcgg cagcggtggt
     3721 agcggtggca gcggtggtag catgcccgag atcgagacag agcaggagta caaggagatt
     3781 ttcatcacac ctcaccagat caagcacatc aaggacttca aggactacaa gtattctcac
     3841 agggtggata agaagcccaa ccgcgagctg atcaatgaca ccctgtatag cacacggaag
     3901 gacgataagg gcaataccct gatcgtgaac aatctgaacg gcctgtacga caaggataat
     3961 gacaagctga agaagctgat caacaagtct cccgagaagc tgctgatgta ccaccacgat
     4021 cctcagacat atcagaagct gaagctgatc atggagcagt acggcgacga gaagaaccca
     4081 ctgtataagt actatgagga gacaggcaac tacctgacaa agtatagcaa gaaggataat
     4141 ggccccgtga tcaagaagat caagtactat ggcaacaagc tgaatgccca cctggacatc
     4201 accgacgatt accctaactc tcgcaataag gtggtgaagc tgagcctgaa gccataccgg
     4261 ttcgacgtgt acctggacaa cggcgtgtat aagtttgtga cagtgaagaa tctggatgtg
     4321 atcaagaagg agaactacta tgaggtgaac agcaagtgct acgaggaggc caagaagctg
     4381 aagaagatca gcaaccaggc cgagttcatc gcctcttttt acaacaatga cctgatcaag
     4441 atcaatggcg agctgtatag agtgatcggc gtgaacaatg atctgctgaa cagaatcgaa
     4501 gtgaatatga tcgacatcac ctacagggag tatctggaga acatgaatga taagaggccc
     4561 cctcgcatca tcaagaccat cgcctctaag acacagagca tcaagaagta cagcacagac
     4621 atcctgggga acctgtatga agtcaagagc aagaaacatc ctcagattat caagaaaggc
     4681 tctggtggtt ctaagcgacc tgccgccaca aagaaggctg gacaggctaa gaagaagaaa
     4741 gattacaaag acgatgacga taagggatcc ggcgcaacaa acttctctct gctgaaacaa
     4801 gccggagatg tcgaagagaa tcctggaccg atggccaagc ctttgtctca agaagaatcc
     4861 accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc
     4921 gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat
     4981 tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct
     5041 ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc
     5101 ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac
     5161 agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg
     5221 gagggctaag aattctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc
     5281 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     5341 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     5401 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     5461 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     5521 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     5581 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     5641 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5701 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5761 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     5821 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     5881 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     5941 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     6001 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     6061 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     6121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     6181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     6241 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     6301 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     6361 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg
     6421 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     6481 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     6541 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     6601 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     6661 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     6721 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     6781 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     6841 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     6901 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     6961 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     7021 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     7081 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     7141 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     7201 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     7261 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     7321 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     7381 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7441 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7501 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     7561 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     7621 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     7681 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     7741 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     7801 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     7861 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     7921 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     7981 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     8041 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     8101 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     8161 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     8221 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     8281 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     8341 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     8401 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     8461 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     8521 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     8581 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     8641 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     8701 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     8761 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     8821 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     8881 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     8941 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     9001 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     9061 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     9121 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     9181 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     9241 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     9301 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     9361 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     9421 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     9481 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     9541 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     9601 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     9661 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     9721 agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     9781 agccagt
//
