LOCUS       sequence-269087-       20076 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pRL623-based helper plasmid for conjugations, with RSF1010 mobA 
            gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20076)
  AUTHORS   Bishe B, Taton A, Golden JW
  TITLE     Modification of RSF1010-Based Broad-Host-Range Plasmids for Improved
            Conjugation and Cyanobacterial Bioprospecting.
  JOURNAL   iScience. 2019 Oct 25;20:216-228. doi: 10.1016/j.isci.2019.09.002. 
            Epub 2019 Sep 10.
  PUBMED    31585408
REFERENCE   2  (bases 1 to 20076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2019-10-25"; volume: "20:216-228. doi"; pages: " 
            10.1016/j.isci.2019.09.002. Epub 2019 Sep 10"
COMMENT     Sequence Label: pAM5505
FEATURES             Location/Qualifiers
     source          1..20076
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     218..237
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     oriT            5323..5410
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             6649..7620
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELSERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     primer_bind     13177..13196
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13417..13434
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     13675..13697
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ctgcaggtcg actctagagt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc
       61 ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga
      121 tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc
      181 tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc
      241 cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg
      301 aactgaacac gtcgcgtgac cgtgaaaaca ccttcgaacc gcagttgatt aagaagcacc
      361 agacgcgcat tacgcagatg gacagccaga ttttatccct ctacgccaaa gatatgacta
      421 cccgcgaaat tgtcgccacc ttcaaagaga tgtacgacgc agatgtgtca cccacgctga
      481 tatgtaaagt cactgatgcg gtcaaagagc aggttgcaga atggcaaaat cgacctctgg
      541 atgcactgta tcccattgtt taccttgact gtatcgtggt gaaagttcgt caaggtggca
      601 ccgtaattaa caaagcggtg ttccttgcac tcggtattaa tactggaggc cagaaagagt
      661 tgctgggcat gtggctggcc gagaacgaag gggcgaagtt ctggctgggg gggctgacag
      721 agcttaagaa tcgggggctt caggatatcc ttattgcctg catggacggt ctgaaaggct
      781 ttccggatgc gataaacagc gtgtatccgt agacgcacat ccagttgtgc atcattcata
      841 tggtgcgcaa cagcctgaaa tacgtatcgt ggaaggacta caaagccgtc actggcgggt
      901 taaaaacggt gtatcaggcg ccaacagaag aggcggcgct agtggcaccg gagcagttcc
      961 cgaatgtctg ggacgataaa tacccgcaaa tcagcaaaag ctggcgtgcg cactgggaaa
     1021 atatcaacac gttcttcggc tattcgcccg atatccgcaa ggccatctac accacgaacg
     1081 ctatcgaatc gctgaacagc gtgatccgtg cggccattaa gaagcgtaaa gtgttcccga
     1141 ctgacgactc gctgcggaag gtcatttctc tggcgatcaa ggatgcatca aaaaaatgga
     1201 atatgccaat ccagagctgg cggctggcga tgagtcgctt tattatcgag ttcggtgacc
     1261 gcctgagcga tcacctttaa tatggcagca attacacaga attatttaca gggtctatct
     1321 tttatttatc ttatctaaaa gatgtaccac agatttgcca acagcaaatg acagattaac
     1381 aggaacagcg tttcctattt gcttatattt cgctgacatt gggccttcaa atacccattc
     1441 atcggggaag gtctgtattc ttgcatactc acgcacagtt aatggtcttg tttcttctgg
     1501 gtggcaacgc tctgtttgtt tctgtgctgg ggcgcatgtt aatgttaggc ttggttcatc
     1561 ccatgacaaa cgacgagcca taccagtttt gcccccacct aagtaaaaac tcttgagcat
     1621 gtattctttt tgaatatctt caggaagatc tctccagtag ccaccgggag gaaccatact
     1681 taggatctct gcttttcttt tgggatattt ttgtccttca gattctggca cgtcgctatc
     1741 atacagctct ccctttttta atgcatcttt aagggttaat attttattgt aagaagaagg
     1801 ccactcataa tcgatgccat cagcaagatc atttcttaca gctacaatga tcaaacgctc
     1861 gcgtttttgc ggcactttgt agaaaatagc cttaagcact cttggctcaa ataaagtgta
     1921 gcccaagtct gtgataatat tttttattgt ttctaaagtt cgtccagcat catgatttag
     1981 caaccctcga acattctctg ctaaaagaac tttcggattg atttctttag cggctcgggc
     2041 gaattcaaag aaaagggtgc cccgtgtatc ttcaaaacca agttttttgc ctgcataaga
     2101 gaatgcctgg caaggaaagc caccagccag cacatcaacg gtattcctat aaggggtgaa
     2161 gtctacttgg ctcacatcac cttcaaccac attccattca ggcctatttt ttcgtaacgt
     2221 cttacaagca tgggagtcaa tttcatttag taaaacagat tttaaaccgg ctttttctaa
     2281 acctaaagcc atcccccctg cgccagcaaa taactctaat acagtataaa cattattgct
     2341 tattttagtc tcatcaggcc actggctttt aaataaaaac tgagcttgtt caaaattttt
     2401 aagttgctct tttttataaa atcgatagtt gttttcgtca tttctttgag aaactaattt
     2461 tccagcagta tcccaacgcc ttaaagtttc ttttgaaacg cccaaaatgt ctgcaacttc
     2521 tgaaagtgaa aactcttcct ttaacatatt tagtaaccat gccaacatta gtcatggtta
     2581 ctactgtatg ggtttacatg tgctttgtca aacattgatc atggttatca gccgtaagcg
     2641 agcccaaaat acttaataaa actatcttta tcagggatgg aaaaaccttg ctgcccaaga
     2701 cgttcgctca aaatatactc aattgcgtga gggagagcac tatggagttc ttgcagagca
     2761 tctggtctgc cagtgacaag ctcatagaaa ctcgcaccat caatgattct aatgttaggg
     2821 ttcgaaggac acagagtacc actaccttta ttggaaggag taaaggggct gttatatctg
     2881 ataggtttac gagggattat attaacaaag tacgcacagt aatccttaaa tcggctatgt
     2941 ttcggtgata ccaactcatc taagccttta tatttatctg ctaaatcccc gccagtaacc
     3001 gttgagtatt tatttttcac ctcagcgatg attcttcttt cttcgttgag aagatccaca
     3061 ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca
     3121 ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt
     3181 gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga
     3241 cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca
     3301 gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac
     3361 tgcgttagca atttaactgt gataaactac cgcattaaag cttctagagg atcccccctt
     3421 tcaatttggc ttactcggat ctttaatgct cttgcctgct caacaaatgg tgttgctttt
     3481 cgatttcttt ctaaagctaa agcaacaaat cctgctcgga cttcttgata tggagtcaca
     3541 agatcatcat tgctttgaat atgatattga tatggcacta gggttttctc caaacataca
     3601 cacatttgcg aagtgtcttt cttccatgtg tccccatttg ttggctactg ttacctttgc
     3661 cagtaggtaa gacaagaatt ttctccacat taaaaccaat ttcttctgca atattagaaa
     3721 gaattaaatc aaccgaaata tcaatacctg catagcgaac attatcattt accataaata
     3781 aaggtgagcc attttttaaa actctaaagc attctataat aacgcaagcc atttcataga
     3841 aatatccttt tatcatacga ggaataccgt tattattaag tttttttctt tcaagctctc
     3901 gctcaagaaa gcgcaagata ctttgtaaca attcttgttt atcaagtatg cgtaatgctt
     3961 cctgccaatt gtgaataaga gacttttctt tgttttcaac agtacaactc agcatatctt
     4021 gcctaagttg tactatatct ctttctccca cacctaatag agctaattct agagcgtatg
     4081 tacgtgtata gtcataacga ttacaatagg gtggagaggt aatgatacag tcgtaaaaat
     4141 cttgctcaaa ttcaggcaga attttaagac atgaagcatt aaataaatta attccacttc
     4201 tttgagtccc ataacaaaat aatgtattac ttattaagtc aaaggaatca ttcaaaatta
     4261 attttatttg ctcagtaatt gcttcatcga aatccagaat tttaccttta tcaaatttat
     4321 ctgatccact tttcctaaat gctcttttat cccaacgtag atactgtcca tctttacggg
     4381 tatagctgat agattcaaga atacacaata gagcaaaacg gagaacttgt ttcacaagaa
     4441 tattctcttt ttctatagaa aataaaaatc tctctattga tgcttctgtt tcaggaggat
     4501 aagctccgtc agtaattctt aaacgattaa gatattttct attattatgc tgattccaag
     4561 gtttttgttt gtaccattca atcaatctca aaaaatcttc tcgacggaat tgtcgctgga
     4621 ttattttcct tacttcaata atctcttgac caataggtaa cacttctata ccatctgctt
     4681 gaataccggc atcactacag gcaaatagca ttgttcctgc tcctgcgaag gggtcaagaa
     4741 tcctctcaaa tgattttttc tcgtactcga atattaactg atttaccaaa tcaactgaaa
     4801 aagcttcttt atatttaaac cagcgatact gaggttttga cttattagct tgaaaactaa
     4861 ctatttttcg acttaaactt tcatgcaatc gcatcttatc tctaaaacgt tgctcaagtg
     4921 ctttattgaa tcgagaaatt tcttgtacaa aggaatcact ctcttgatca agatcagtcg
     4981 ggtcagtctt tatttctatt ggactctcaa gctcaaatga agtcatttta tatttttctt
     5041 gtaacaaaga ggtaacttaa gattctatca ggacaaagca ctatcgtcta tcacttaaga
     5101 tttgaaccct attttttata gactaattga caacagttag aacctgatta tactttgtta
     5161 tacttctaca gtaatcaagt taaatattga atagggtacc gagcttcagg gctgaatttc
     5221 ggcattgatt cgcgctcgtt gttcttcgag cttggccagc cgatccgccg ccttgttgct
     5281 ccccttaacc atcttgacac cccattgtta atgtgctgtc tcgtaggcta tcatggaggc
     5341 acagcggcgg caatcccgac cctactttgt aggggagggc gcacttaccg gtttctcttc
     5401 gagaaactgg cctaacggcc acccttcggg cggtgcgctc tccgagggcc attgcatgga
     5461 gccgaaaagc aaaagcaaca gcgaggcagc atggcgattt atcaccttac ggcgaaaacc
     5521 ggcagcaggt cgggcggcca atcggccagg gccaaggccg actacatcca gcgcgaaggc
     5581 aagtatgccc gcgacatgga tgaagtcttg cacgccgaat ccgggcacat gccggagttc
     5641 gtcgagcggc ccgccgacta ctgggatgct gccgacctgt atgaacgcgc caatgggcgg
     5701 ctgttcaagg aggtcgaatt tgccctgccg gtcgagctga ccctcgacca gcagaaggcg
     5761 ctggcgtccg agttcgccca gcacctgacc ggtgccgagc gcctgccgta tacgctggcc
     5821 atccatgccg gtggcggcga gaacccgcac tgccacctga tgatctccga gcggatcaat
     5881 gacggcatcg agcggcccgc cgctcagtgg ttcaagcggt acaacggcaa gaccccggag
     5941 aagggcgggg cacagaagac cgaagcgctc aagcccaagg catggcttga gcagacccgc
     6001 gaggcatggg ccgaccatgc caaccgggca ttagagcggg caggccacga cgcccgcatt
     6061 gaccacagaa cacttgaggc gcagggcatc gagcgcctgc ccggtgttca cctggggccg
     6121 aacgtggtgg agatggaagg ccggggcatc cgcaccgacc gggcagacgt ggccctgaac
     6181 atcgacaccg ccaacgccca gatcatcgac ttacaggaat accgggaggc aatagaccat
     6241 gaacgcaatc gacagagtga agaaatccag aggcatcaac gagttagcgg agcagatcga
     6301 accgctggcc cagagcatgg cgacactggc cgacgaagcc cggcaggtca tgagccagac
     6361 ccagcaggcc agcgaggcgc aggcggcgga gtggctgaaa gcccagcgcc agacaggggc
     6421 ggcatgggtg gagctggcca aagagttgcg ggaggtagcc gccgaggtga gcagcgccgc
     6481 gcagagcgcc cggagcgcgt cgcgggggtg gcactggaag ctatggctaa ccgtgatgct
     6541 ggcttccatg atgcctacgg tggtgctgct gatcgcatcg ttgctcttgc tcgacctgac
     6601 gccactgaca accgaggacg gctcgatctg gctgcgcttg gtggcccgat gaagaacgac
     6661 aggactttgc aggccatagg ccgacagctc aaggccatgg gctgtgagcg cttcgatatc
     6721 ggcgtcaggg acgccaccac cggccagatg atgaaccggg aatggtcagc cgccgaagtg
     6781 ctccagaaca cgccatggct caagcggatg aatgcccagg gcaatgacgt gtatatcagg
     6841 cccgccgagc aggagcggca tggtctggtg ctggtggacg acctcagcga gtttgacctg
     6901 gatgacatga aagccgaggg ccgggagcct gccctggtag tggaaaccag cccgaagaac
     6961 tatcaggcat gggtcaaggt ggccgacgcc gcaggcggtg aacttcgggg gcagattgcc
     7021 cggacgctgg ccagcgagta cgacgccgac ccggccagcg ccgacagccg ccactatggc
     7081 cgcttggcgg gcttcaccaa ccgcaaggac aagcacacca cccgcgccgg ttatcagccg
     7141 tgggtgctgc tgcgtgaatc caagggcaag accgccaccg ctggcccggc gctggtgcag
     7201 caggctggcc agcagatcga gcaggcccag cggcagcagg agaaggcccg caggctggcc
     7261 agcctcgaac tgtccgagcg gcagcttagc cgccaccggc gcacggcgct ggacgagtac
     7321 cgcagcgaga tggccgggct ggtcaagcgc ttcggtgatg acctcagcaa gtgcgacttt
     7381 atcgccgcgc agaagctggc cagccggggc cgcagtgccg aggaaatcgg caaggccatg
     7441 gccgaggcca gcccagcgct ggcagagcgc aagcccggcc acgaagcgga ttacatcgag
     7501 cgcaccgtca gcaaggtcat gggtctgccc agcgtccagc ttgcgcgggc cgagctggca
     7561 cgggcaccgg caccccgcca gcgaggcatg gacaggggcg ggccagattt cagcatgtag
     7621 tgcttgcgtt ggtactcacg cctgttatac tatgagtact cacgcacaga agggggtttt
     7681 atggaatacg aaaaaagcgc ttcagggtcg gtctacctga gctcgaattc atcggtaggg
     7741 agattttttc aagtaacaga ggttttggat atttaaaaaa tatttccttg atttcgataa
     7801 aacgaataag ttccctatga gctttgttgt taaaactgag cttgagccag aagaggttat
     7861 ggacaagata aagattaact ctgaaaaaag catggaaaag gaattggtgc agaaatatct
     7921 gattttgttt gaagaaattt ttacattaca gacgctcaga agtatcctgg gaatgatatc
     7981 tgactctctt gatgagcttg aagagctaaa gagagtcata atcgaaaatt ttaaattaga
     8041 gtatggtctt cttgattaaa taataaatca ttgtttaatg ggttagagat gttgtcaata
     8101 tcatctctaa tgttttttga tttgtttttt gtcttccttt tttttacttt ctccaattct
     8161 tttggggtat aatctgaagg tatacccaat cttcttatgc ccatttttac atactcttcg
     8221 tttatttcta tgcctataaa tctcctgtct agagacttgg caacatctcc tgttgtaaag
     8281 ctaccagaaa aagggtctaa gactacatcg cctgggttcg atgaagctag aataattctc
     8341 ctcaaaaggg acgtcggttt ctgtgtgggg tggttttcat attcctccat aagaaatctt
     8401 actctaggaa aatcccaaac gtttcctggt actttttgtg tgctataagg ttggggtggg
     8461 ttttttctat aatcaatcaa agccctttta gatcctgttt ttgcttcaac caaaatatct
     8521 ttgctgttaa atgtgtattt tttttgatct tttaccatca ttaagattgg ttcgtacatg
     8581 gagccaaagt gttttttggc ttgaacacct gagctgtcat aagaccaaac taatcgactt
     8641 tttatatgga agatttttct acattttatg tcaatgtatg gcatattctc agtgctgttc
     8701 ataatataca tagtgccatg cttttttagc actctatggc attcatcgac gcattcgaat
     8761 aaccaatgaa ggaattcacc ctcatcccat gactcaacaa gtccgtcaaa gtccttacca
     8821 atattatatg gggggtctgc aaaaattaaa tcaacactct ctgctggcaa tttctttaat
     8881 tcagtcaacg catcgccatg gataatttta tttttttcat ctttaaaata tctagcttca
     8941 aaatctattt tcattttcta cctcttgtgt gactcatagt ctactatgtt accgatatct
     9001 agatctcgag ctatcaggct agagatttta ccgccaaatc gaaccttatt agagcggttt
     9061 aggctggacc ggcagttaaa attggggctt gagcggtaaa cgagtgaggg aatttcaggt
     9121 aagatacttc ggatgaggag caaaaaggtg gtttatactt cctataccca aaaaggtggt
     9181 ttatacttcc tataccccgg ttatccggtg gttttctgtt tgcggaataa aacagattag
     9241 tgacagtttt ctcagaatgg cgtgttccgg tgacttctgt tttcgtgtgc tgcatccgga
     9301 agtgtcaggc ccgccggata tgaaaaaggc ctccggagag taccgtgact ttattttcgg
     9361 cacaaataca ggggtcgatg gataaatacg gcgatagttt cctgacggat gatccgtatg
     9421 taccggcgga agacaagctg caaacctgtc agatggagat tgatttaatg gcggatgtgc
     9481 tgagagcacc gccccgtgaa tccgcagaac tgatccgcta tgtgtttgcg gatgattggc
     9541 cggaataaat aaagccgggc ttaatacaga ttaagcccgt atagggtatt attactgaat
     9601 accaaacagc ttacggagga cggaatgtta cccattgaga caaccagact gccttctgat
     9661 tattaatatt tttcactatt aatcagaagg aataaccatg aattttaccc ggattgacct
     9721 gaatacctgg aatcgcaggg aacactttgc cctttatcgt cagcagatta aatgcggatt
     9781 cagcctgacc accaaactcg atattaccgc tttgcgtacc gcactggcgg agacaggtta
     9841 taagttttat ccgctgatga tttacctgat ctcccgggct gttaatcagt ttccggagtt
     9901 ccggatggca ctgaaagaca atgaacttat ttactgggac cagtcagacc cggtctttac
     9961 tgtctttcat aaagaaaccg aaacattctc tgcactgtcc tgccgttatt ttccggatct
    10021 cagtgagttt atggcaggtt ataatgcggt aacggcagaa tatcagcatg ataccagatt
    10081 gtttccgcag ggaaatttac cggagaatca cctgaatata tcatcattac cgtgggtgag
    10141 ttttgacgga tttaacctga acatcaccgg aaatgatgat tattttgccc cggtttttac
    10201 gatggcaaag tttcagcagg aaggtgaccg cgtattatta cctgtttctg tacaggttca
    10261 tcatgcagtc tgtgatggct ttcatgcagc acggtttatt aatacacttc agctgatgtg
    10321 tgataacata ctgaaataaa ttaattaatt ctgtatttaa gccaccgtat ccggcaggaa
    10381 tggtggcttt tttttatatt ttaaccgtaa tctgtaattt cgtttcagac tggttcagga
    10441 tcactgtacg ataatgcccc cgcagtttgg taataccctt aataaaaaag aaacagcaaa
    10501 gactgacagc aataataata aagtaagcag taacaataat attaacaaca ccagatgcag
    10561 ttataataat agtatttaag acaccagaaa gactgctgcg acagtcattt tgaacaacac
    10621 caaaatgccg taaaggcagt agtaacaaca ccagtgaaaa catcacgata gcatagtgat
    10681 atgcctgagt gtgtgtaatt aaacaataaa taaaccgcca tatataacag aagatagtat
    10741 tctgaatggc atgcttttct gttcagtata aacatatcat cccggttggt ataaggatga
    10801 tatataataa gttaagctga acacatattt attttggttt tattttacaa ataaagtaag
    10861 acgatccgtt aagtcaaagc ggggtatatt tattataccc tgctttttta tttgtccgcc
    10921 gggcgcggat aatggatcag attatgcagt gtcacaatgg ccttaccggg attggcgtaa
    10981 gcgtgcggga tatccgcatg gaagcgcagg gattccccgg cagaaacggt gtgccactca
    11041 tcccccagcc gcagttgtaa tgcgccttcc agtacaatga catgttctct ggttctgaaa
    11101 tccatccctg tcggtgttgc ttatgcagtc tggtcgggac tcggcgtcgt cataattaca
    11161 gccattgcct ggttgcttca tgggcaaaag cttgatgcgt ggggctttgt aggtatgggg
    11221 ctcataattg ctgccttttt gctcgcccga tccccatcgt ggaagtcgct gcggaggccg
    11281 acgccatggt gacggtgttc ggcattctga atctcaccga ggactccttc ttcgatgaga
    11341 gccggcggct agaccccgcc ggcgctgtca ccgcggcgat cgaaatgctg cgagtcggat
    11401 cagacgtcgt ggatgtcgga ccggccgcca gccatccgga cgcgaggcct gtatcgccgg
    11461 ccgatgagat cagacgtatt gcgccgctct tagacgccct gtccgatcag atgcaccgtg
    11521 tttcaatcga cagcttccaa ccggaaaccc agcgctatgc gctcaagcgc ggcgtgggct
    11581 acctgaacga tatccaagga tttcctgacc ctgcgctcta tcccgatatt gctgaggcgg
    11641 actgcagatg gaatgcgctt tgttacgcca gtcagaacaa tcaatgccgg accgaaccgc
    11701 aaatactttg gtaataacag agggatcacc tggtacaact ttgtgtccga tcagtattcc
    11761 ggctttcatg gcatcgttat accggggacg ctgagggact ctatctttgt gctggaaggc
    11821 cttctggaac aggagaccgg gctgaatcca accgaaatta tgaccgatac agcaggtacc
    11881 agcgaacttg tctttggcct tttctggctg ctgggatacc agttttctcc acgcctggct
    11941 gatgccggtg cttcggtttt ctggcgaatg ggccatgatg ccaactatgg cgtgctgaat
    12001 gatattgcca gagggcaatc agatccccga aaaatagtcc ttcagtggga cgaaatgatc
    12061 cggaccgctg gctccctgaa actgggcaaa gtacaggctt cagtgctggt ccgttcattg
    12121 ctgaaaagtg aacgtccttc cggactgact caggcaatca ttgaagtggg gcgcatcaac
    12181 aaaacgctgt atctgcttaa ttatattgat gatgaagatt accgccggcg cattctgacc
    12241 cagcttaatc ggggagaaag ccgccatgcc gttgccagag ccatctgtca cggtcaaaaa
    12301 ggtgagataa gaaaacgata taccgacggt caggaagatc aactgggcgc actggggctg
    12361 gtcactaacg ccgtcgtgtt atggaacact atgtatatgc aggcagccct ggatcatctc
    12421 cgggcgcaag gtgaaacact gaatgatgaa gatatcgcac gcctctcccc gctttgccac
    12481 ggacatatca atatgctcgg ccattattcc ttcacgctgg cagaactggt gaccaaagga
    12541 catctgagac cattaaaaga ggcgtcagag gtagaaaacg ttgcttaacg tgagttttcg
    12601 ttccactgag cgtcagaccc ctgtacaagg aggaaccgtt gcaccatctt cgtcttctga
    12661 actgacgggg atcgcggttc agtagaaaag atcaaaggat cttcttgaga tccttttttt
    12721 ctgcgcgtaa tctgctgcct gtaaacgaaa aaaccgcctg gggaggcggt ttagtcgaag
    12781 gttaagtcag ttggggactg cttaaccggg taactggctt tagtggagca cagataccaa
    12841 atgctgtcct tccagtgtag ccgctataag gcttcaactt caagagttct gcgtatctaa
    12901 atccactatc cagttaccaa tggctgctgc cagtggcgtt aagtcgtgtc ttaccgggtt
    12961 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
    13021 cacacagccc agcttggagc gaacgaccta caccgaactg agataccaac agcgtgagct
    13081 atgagaaagc gccacgcttc ccaaagggag aaaggcggac aggtatccgg taagcggcag
    13141 ggtcggaaca ggagagcgca cgagggagcc accaggggga aacgcctggt atctttaagt
    13201 cctgtcgggt ttcgccacct ctgacttgag cgtcggtttt tgtgatgctc gtcagggggg
    13261 cggagcctat ggaaaaacgc cagctgcgtg gccttttccg ggttcttgtt ttttgctcac
    13321 atgtttcttt tggtgttatc ccctgattct gtggataacc gcattaccgt ctttgagtga
    13381 gctgataccg ctcgccgcag ccgaacgacc gagcggagcg agtcagtgag cgaggaagcg
    13441 gaaaagcgcc tggacgagca ttttctcctt acgcatctgt gcggcatttc acacccggca
    13501 tggtgcactt ttcatacaat cctcactgat gccaaatgat taagccagta tacactccgc
    13561 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gctaaaaccc gctgacgcgc
    13621 cctgacgggc ttgtctgctc ccggcatccg ctcacaggcc agctgtgacc gtctccggga
    13681 gctgcatgtg tcagaggttt tcaccgttat ccccgaagcg tgcgaggcag ctgcggtaaa
    13741 gctcgtcagc gtgatcgtga agcgcttcgc agatgtcagc ctggtcgtcg gcgtccagtt
    13801 cattcagttt ttccagcagg gtcagcgtct ggcttctgat gaagcgggcc atgttaaggg
    13861 cggttttttc ctgtttagtc acagttctgt ttctccatgt gtggggagtc tgtcggaggt
    13921 ttttaatgat accggtgaaa cgcgaaagga tgctcacgat acgggtcacg gatgaggaac
    13981 atgcgcggct gctggcacgt tgtgagggaa aacaacttgc gtcatggatg cgtaaggtct
    14041 gcctgggggc accgccatcg aaaacgtcag gtcttccgac actggcaccg ccgctgttgc
    14101 gccagttcgc ttcggtcggc aataacctga atcagatagc ccggaagata aacagcggac
    14161 agtggtcggg gcatgaccgg gttcatgtgg tggctgcact gatggccatc ggacgtgaac
    14221 tgagtgagct gagggatgag gtccgtaaac agggagaacg ggatgatagt taagtttcat
    14281 gccaggggaa aaggcggtgg cagtggtccg gttgattacc tgctgggccg ggagcgtaac
    14341 cgggaagggg cgagagttct gcggggtgct cccgaagagg tgcgggaact catcgatgcc
    14401 acgccgtttg cgaaaaaata cacctcgggg gttctgtctt ttgcagagca gaccctgcca
    14461 ccgggagagc gtgaaagggt gatggagagc tttgagcggg tactgatgcc cggtcttgaa
    14521 aaaaatcagt acagcatcct gtgggtggag caccaggaca aggggcgtct tgagctgaat
    14581 tttgtcatcc cgaatatgga gctggcgagc ggaaaacggc tccagcccta ttacgaccga
    14641 gcggaccgtc cgcgaatcaa tgcctggcag acactggtga atcaccatta cgggctgcac
    14701 gacccgaacg caccggagaa caggcggaca cttgtcaccc cgaataatct gccgaaagcg
    14761 aaacaggagg ccgcagaagc gattacgagg ggactggagg ctctttacca tgccggagcg
    14821 ctaaaaacgc gtcaggatgt cactgaggcg cttacagcgg caggttttga ggttgtgagg
    14881 acaaccagga gcagtatcag tatcgccgac ccggagggtg ggcgtaatct caggctgaaa
    14941 ggggcgctgt atgagcaatc ttttacaaat ggggacagac ttcgagaaga aactgaaaga
    15001 gcgtgccgtc tctacagaga acatgctgaa cagcgagttc aggaagctcg aagcgtctgt
    15061 agacagggaa ctgaacttaa acgggcagaa aatcagagac gctatcagcg cacacacagc
    15121 agcggtgaaa gaacaactgg agacgctgaa cagtgccgtg aacgcgcagt tcagcgccac
    15181 agagaacgaa ctggcccggc ggcaggaaga gctgctctgg aagctggtca aagggcggat
    15241 actgtacccg tcgctgacgg cgctttgcgt cacgggcggg atttttctgg cgtcatgggg
    15301 gctgattcag tggcaggaaa gccggattgc ggcgaacatc ctggctatca gggaccagga
    15361 agagacgctg gcgaaactcc gggaaaaaac atggggtgtg acgtatcacg agggcaggaa
    15421 cgggaaattt ctagtgttgc cgtccggggt gaagggggaa aacaactgga cggtggagaa
    15481 gaagaacgca gtaagactgg tcagggagta agtgagtatg acagagctgg aaatgcactt
    15541 actgaacgcc ttagagcaat tgcagcagga ctatatgcag cggctgaaag actgggagag
    15601 cgccttcacg gatttgcagc atatgttcga gctgacacga caggagaacg ggcaactgag
    15661 ggaacagtgc gacgacttga gtcagcaggt gcagagatta gccgggcagg tggaccactt
    15721 gagtcggtta tttatcacga acagcagata agggatgaac aggaaaggca ggcacgcctg
    15781 gaacgggagc gtgcgcggtc ctggcatgaa gagcaggaac gggaagcacg ggtatggcgt
    15841 ggcccgtcaa taggtatgtg acggtgagtt gttccgggag gttgccagat ggtaccggaa
    15901 cgcgttaaaa acgcatccgg gcgcgattct ggcgtgtcgt ttttgttgct gtgttttgcc
    15961 tctttgccag atcgcgctca ttcctcagcc tcatcccgcg atgtctgcgt atgtcatcaa
    16021 ctgaaaagat ggaaaaaaaa cagtgtcatg aggtttacgc aggcaaaacc agcgttattc
    16081 acatggctga atagctatgc atacggttaa aatttatcag gtgcgatcgc ggcagctttt
    16141 cgggtggttt gttgccattt ttacctgtca gcagccgtga tcgcgctgaa cgcatttcag
    16201 cggtgcgtac aattaaggga ttatggtaaa tcccttactg tctgccctcg tatccatcga
    16261 gataaaccgc agaaatggtg tctgccatta gtttcatttt tttcggcgaa ggcatatcgc
    16321 tgtttgtgta ttttctgcca gcaaaggctg ttgtgctccg gagtgagtga aaaaattttt
    16381 ctgagtggaa ttcttttaca cgttgctgtg ccgtttctgc tgatactccg gctgctttgt
    16441 ctttcatccc catatcccgg aactgctgaa agaacgggag catcatacgt atggtgtctg
    16501 ctttctgggt gtagaagtta ggatcaaatc catcatctga agcataaaca taatgccggg
    16561 tatgtcgttc caggctggtc tttggtgtgc atcctgtaag agctattgtt gctgttatca
    16621 gaggtatcag aagcagcctg gcagcggcag atctcataaa catctccttg cgtttttaat
    16681 cttgatactc agggagttat atctccgaag agtatctctt atcatacgtc ggtttactcc
    16741 caatgtgatt tgatggtcaa tgagtcagga ataatcccgt gaaaaaatgt gtgccagtcc
    16801 tgctttttag tgttctttct gcttcggttg ccagtcatgc ggacagcaat gtttttcaac
    16861 aaaaggccga gaagacaaaa aacggcattg ttgcggatgt taatgccaga cagaaaatgt
    16921 tcagtaatag actgaagata caacttcaga tcgatgagtt gagagcagag ctggctggca
    16981 ccagagatgc ccaaaaaaga gaggatttgc gtaaaaaact ggagtctctg gagcggcaaa
    17041 agaaatcctt acgctgatgg gggcgtattt tccccggttt cttccataac ctttttaccg
    17101 ttcagatatt tacgggcgag gcgaatgcgt cttgcctggt ataccgccga gtaagcctgg
    17161 gtgggattca caccattact gatttcactg accacatgac gctgatcagc cagtgtcagc
    17221 gtgtaaacct gtttctggat gaggtcacgg gctttgtcat gctcatatcc ggcagcaaca
    17281 agggtgtcga gaagtcgttt gtctgttttg ttcagtttat ggcggggtga tttgctcaca
    17341 tcgttctccg ttttttcatg atccaacagt tttatatctc atcctgattt gctccggtgt
    17401 tctgtttcga cgttctcaat gcctgcctcc ccatagtgtg atccttattg caaaaatgaa
    17461 aatccatgct cttgacatgg acaatgctga gtagtaggtt tttactgtac ataaaaccag
    17521 tggttatatg tacagtatat tgtttttaat ttattgtttt aattatcaaa gaggaattta
    17581 tggctaaaga actaagtgga tatggaccaa ctgctggtga gtctatgggg ggaacagggg
    17641 cgaatctgaa tcagcaaggt ggaaataata atagtaatag tggggttcac tggggtggtg
    17701 gctctgggca tggtaataat ggtggtcagg gaaattcaaa ttcatctggt tctacatcaa
    17761 ctgtgatgaa aaccggtgag tcatatctga ctccgtgggg tgatgttgtc attaataatg
    17821 atggccttcc ggtgatgaat ggtattgtta tgacagaaga gaattcaact ctggttgata
    17881 atccatttgg aggggtctcc agagttctta attctttaat tagtgatatg ccatcacttt
    17941 ttgcagaaag ctcaggaaat aacaacaata acactgcttc agttaatact gcaccaacga
    18001 atgctcaggt cagtgatatg gataagagtt cgaaggtggt gagtaatgta attaatgaaa
    18061 aacaaaagca gaagaataaa attgctacac agattagtga aaaacagaag aaaatcgagg
    18121 agatgaaaaa agtattcaag catcattctt atcatggtat cactgatctt gagagggatg
    18181 ttgatgagtt gcaaaaaaaa tcaaatcagc ttgatgcaga tattagcaaa cttaattctt
    18241 ataaaaatac attgcaaagt aaaattggtg atgtgaataa acagaaggaa gctgaagaaa
    18301 aggctcgtga aaatgcagag gtcgctgaac atgaaactct taatgaagag aaacaggcgg
    18361 tggcggaagc agagaagcgt ttagctgagg cgaaagctga actggcgaag gctgaaagtg
    18421 atgtacagag taagcaagct actgtttcca gagttgcagg agagcttgaa aacgctcaga
    18481 agagtgttga cgtgaaggtc accggatttc cgggatggcg tgacgttcag aaaaaactac
    18541 agcgtcagct tgaagctaag caggctgagt actccgcagt tgaaaatgaa cttaagaatg
    18601 ctgttagttt cagggatggt aaggccgctg aggttaaaga ggcagagcag aaattaaaag
    18661 aagctcagga tgctcttgaa aagagccaga taaaagatgc tgttgatact atggttggat
    18721 tttatcaata tataaccgaa caatatggag aaaaatacgc aaaaattgct caggatttag
    18781 cggaaaagtc taaaggaaag aaaattcaag gtgttgatga agctctggca gcatttgaaa
    18841 agtataagaa tgtactggat aagaaattca gtaaggttga tcgggatgct atttttaatg
    18901 cgttagagtc tgttaattat gatgagttat ctaagaattt aacgaagata tcaaagtcgc
    18961 ttaaaatcac cagtcgagta tcttttctat atgatgttgg ttctgatttt aaaaatgcaa
    19021 tagaaactgg taactggcga cctttgtttg tcacgctgga gaaaagtgcg gtcgatgtag
    19081 gtgtggcaaa aattgtggca ttaatgttta gttttattgt tggtgtgcct cttggtttct
    19141 gggggattgc aattgtcaca ggtattgttt cttcttacat aggggatgat gagttgagca
    19201 aacttaatga gttgttaggg atttaatttc tctttagagt gtaatatatg aaacttggca
    19261 atcctactgg gattgccatt aataaataaa acaatgcaga taacttgcat aagtaccagg
    19321 gttctttttt taactttgta ttttctttga tatatctaga tatggttaat ttagagtatg
    19381 gatatagcaa tacatgtatt gatgatataa atataaataa caaaggagtg ttatcattgt
    19441 cgttaaatat taaaataaga atccatggta taagtgattc aggtaaatta tgtaggtagt
    19501 attttaaatg cataagtatt tttacttttg ttggaaagaa atatgaaaat aataattttt
    19561 ctaattacaa tattattctt gtcaggatgt caagcaaact atattcgtga tgtacggggt
    19621 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    19681 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    19741 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    19801 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    19861 gggagggctt accatctggc cccagtgctg caatgatacc gcgagatcca cgctcaccgg
    19921 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    19981 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    20041 cgccagttaa tagtttgcgc aacgttgttg ccattg
//
