LOCUS       sequence-269091-       11666 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of BRSK2 (human) tagged with C-terminal 
            V5-TurboID for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11666)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-BRSK2-V5-TurboID_IDG-K
FEATURES             Location/Qualifiers
     source          1..11666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1983..2099
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2131..2151
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2151..3362
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3199..3218
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    3363..3387
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5422..5446)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5448..5489
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5496..6452
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(6458..6478)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    6507..7095
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6560..6580)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6978..6989)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7109..7608
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7144..7167)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7541..7560
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7629..8228
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7629..7648)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8125..8145
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8366..8599
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8671..8792
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8708..8727)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8762..8781
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8832..8967
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8987..9006)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8988..9006)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9016..9033)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9016..9032)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9025..9047)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9174..9629
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9261..9280)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9471..9492
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9655..9759
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9760..9828,9829..10620)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10791..11379
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11280..11299
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11533..11550
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta
     2101 tcgtccagtg tggtggaatt ctgcagatat caactttgta tagaaaagtt ggcctccgcg
     2161 ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga
     2221 cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg
     2281 ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt
     2341 gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc
     2401 ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa
     2461 ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt
     2521 gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct
     2581 ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt
     2641 agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg
     2701 gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa
     2761 acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg
     2821 ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt
     2881 ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg
     2941 ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac
     3001 ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt
     3061 ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc
     3121 cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta
     3181 cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg
     3241 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt
     3301 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag
     3361 acgcaacttt gtacaaaaaa gcagaagggc cgtcaaggcc caccatgaca tcgacgggga
     3421 aggacggcgg cgcgcagcac gcgcagtatg ttgggcccta ccggctggag aagacgctgg
     3481 gcaaggggca gacaggtctg gtgaagctgg gggttcactg cgtcacctgc cagaaggtgg
     3541 ccatcaagat cgtcaaccgt gagaagctca gcgagtcggt gctgatgaag gtggagcggg
     3601 agatcgcgat cctgaagctc attgagcacc cccacgtcct aaagctgcac gacgtttatg
     3661 aaaacaaaaa atatttgtac ctggtgctag aacacgtgtc aggtggtgag ctcttcgact
     3721 acctggtgaa gaaggggagg ctgacgccta aggaggctcg gaagttcttc cggcagatca
     3781 tctctgcgct ggacttctgc cacagccact ccatatgcca cagggatctg aaacctgaaa
     3841 acctcctgct ggacgagaag aacaacatcc gcatcgcaga ctttggcatg gcgtccctgc
     3901 aggttggcga cagcctgttg gagaccagct gtgggtcccc ccactacgcc tgccccgagg
     3961 tgatccgggg ggagaagtat gacggccgga aggcggacgt gtggagctgc ggcgtcatcc
     4021 tgttcgcctt gctggtgggg gctctgccct tcgacgatga caacttgcga cagctgctgg
     4081 agaaggtgaa gcggggcgtg ttccacatgc cgcactttat cccgcccgac tgccagagtc
     4141 tgctacgggg catgatcgag gtggacgccg cacgccgcct cacgctagag cacattcaga
     4201 aacacatatg gtatataggg ggcaagaatg agcccgaacc agagcagccc attcctcgca
     4261 aggtgcagat ccgctcgctg cccagcctgg aggacatcga ccccgacgtg ctggacagca
     4321 tgcactcact gggctgcttc cgagaccgca acaagctgct gcaggacctg ctgtccgagg
     4381 aggagaacca ggagaagatg atttacttcc tcctcctgga ccggaaagaa aggtacccga
     4441 gccaggagga tgaggacctg cccccccgga acgagataga ccctccccgg aagcgtgtgg
     4501 actccccgat gctgaaccgg cacggcaagc ggcggccaga acgcaaatcc atggaggtgc
     4561 tcagcgtgac ggacggcggc tccccggtgc ctgcgcggcg ggccattgag atggcccagc
     4621 acggccagag gtctcggtcc atcagcggtg cctcctcagg cctttccacc agcccactca
     4681 gcagcccccg ggtgacccct cacccctcac caaggggcag tcccctcccc acccccaagg
     4741 ggacacctgt ccacacgcca aaggagagcc cggctggcac gcccaacccc acgcccccgt
     4801 ccagccccag cgtcggaggg gtgccctgga gggcgcggct caactccatc aagaacagct
     4861 ttctgggctc accccgcttc caccgccgga aactgcaagt tccgacgccg gaggagatgt
     4921 ccaacctgac accagagtcg tccccagagc tggcgaagaa gtcctggttt gggaacttca
     4981 tcagcctgga gaaggaggag cagatcttcg tggtcatcaa agacaaacct ctgagctcca
     5041 tcaaggctga catcgtgcac gccttcctgt cgattcccag tctcagccac agcgtcatct
     5101 cccaaacgag cttccgggcc gagtacaagg ccacgggggg gccagccgtg ttccagaagc
     5161 cggtcaagtt ccaggttgat atcacctaca cggagggtgg ggaggcgcag aaggagaacg
     5221 gcatctactc cgtcaccttc accctgctct caggccccag ccgtcgcttc aagagggtgg
     5281 tggagaccat ccaggcccag ctgctgagca cacacgaccc gcctgcggcc cagcacttgt
     5341 cagacaccac taactgtatg gaaatgatga cggggcggct ttccaaatgt ggaattatcc
     5401 cgaaaagttc aggcctcatg ggcccagctt tcttgtacaa agtgggcggc aagcccatcc
     5461 ccaaccccct gctgggcctg gacagcaccg ctagcaaaga caatactgtg cctctgaagc
     5521 tgatcgctct cctggctaat ggcgagttcc atagtggcga acagctggga gaaaccctgg
     5581 gcatgtccag ggccgctatc aacaagcaca ttcagactct gcgcgactgg ggcgtggacg
     5641 tgttcaccgt gcccggaaag ggctactctc tgcccgagcc tatcccgctg ctgaacgcta
     5701 aacagattct gggacagctg gacggcggga gcgtggcagt cctgcctgtg gtcgactcca
     5761 ccaatcagta cctgctggat cgaatcggcg agctgaagag tggggatgct tgcattgcag
     5821 aatatcagca ggcagggaga ggaagcagag ggaggaaatg gttctctcct tttggagcta
     5881 acctgtacct gagtatgttt tggcgcctga agcggggacc agcagcaatc ggcctgggcc
     5941 cggtcatcgg aattgtcatg gcagaagcgc tgcgaaagct gggagcagac aaggtgcgag
     6001 tcaaatggcc caatgacctg tatctgcagg atagaaagct ggcaggcatc ctggtggagc
     6061 tggccggaat aacaggcgat gctgcacaga tcgtcattgg cgccgggatt aacgtggcta
     6121 tgaggcgcgt ggaggaaagc gtggtcaatc agggctggat cacactgcag gaagcaggga
     6181 ttaacctgga caggaatact ctggccgcta cgctgatccg agagctgcgg gcagccctgg
     6241 aactgttcga gcaggaaggc ctggctccat atctgccacg gtgggagaag ctggataact
     6301 tcatcaatag acccgtgaag ctgatcattg gggacaaaga gattttcggg attagccggg
     6361 ggattgataa acagggagcc ctgctgctgg aacaggacgg agttatcaaa ccctggatgg
     6421 gcggagaaat cagtctgcgg tctgccgaaa agtaagccaa ctttattata catagttgat
     6481 atccagcaca gtggcggccg ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     6541 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     6601 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     6661 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     6721 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     6781 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     6841 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     6901 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     6961 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     7021 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     7081 gggccgcctc cccgcctgga attctaccgg gtaggggagg cgcttttccc aaggcagtct
     7141 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
     7201 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc
     7261 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
     7321 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc
     7381 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
     7441 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc
     7501 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag
     7561 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca
     7621 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     7681 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     7741 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     7801 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     7861 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     7921 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     7981 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     8041 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     8101 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     8161 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     8221 gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg
     8281 catctcgagg gcccggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     8341 cactttttaa aagaaaaggg gggactggaa gggctagctc actcccaacg aagacaagat
     8401 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     8461 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     8521 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     8581 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     8641 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     8701 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     8761 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     8821 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     8881 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     8941 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     9001 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     9061 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     9121 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     9181 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     9241 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     9301 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     9361 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     9421 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     9481 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     9541 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     9601 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     9661 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     9721 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     9781 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     9841 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     9901 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     9961 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
    10021 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
    10081 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
    10141 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
    10201 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
    10261 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
    10321 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
    10381 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
    10441 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
    10501 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
    10561 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
    10621 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
    10681 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
    10741 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    10801 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    10861 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    10921 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
    10981 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    11041 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    11101 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    11161 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    11221 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    11281 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    11341 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    11401 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
    11461 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
    11521 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
    11581 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
    11641 aaagcgggca gtgagcgcaa cgcaat
//
