LOCUS       sequence-269093-       11783 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of BRSK1 (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11783)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-BRSK1-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..11783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1983..2099
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2131..2151
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2151..3362
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3199..3218
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    3363..3387
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5725..5749)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5751..5792
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5799..6569
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(6575..6595)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    6624..7212
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6677..6697)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7095..7106)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7226..7725
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7261..7284)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7658..7677
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7746..8345
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7746..7765)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8242..8262
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8483..8716
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8788..8909
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8825..8844)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8879..8898
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8949..9084
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9104..9123)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9105..9123)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9133..9150)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9133..9149)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9142..9164)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9291..9746
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9378..9397)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9588..9609
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9772..9876
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9877..9945,9946..10737)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10908..11496
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11397..11416
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11650..11667
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta
     2101 tcgtccagtg tggtggaatt ctgcagatat caactttgta tagaaaagtt ggcctccgcg
     2161 ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga
     2221 cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg
     2281 ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt
     2341 gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc
     2401 ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa
     2461 ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt
     2521 gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct
     2581 ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt
     2641 agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg
     2701 gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa
     2761 acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg
     2821 ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt
     2881 ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg
     2941 ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac
     3001 ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt
     3061 ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc
     3121 cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta
     3181 cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg
     3241 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt
     3301 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag
     3361 acgcaacttt gtacaaaaaa gcaggcttaa tgtcgtccgg ggccaaggag ggaggtgggg
     3421 gctctcccgc ctaccacctc ccccaccccc acccccaccc accccagcac gcccaatatg
     3481 tgggccccta tcggctggag aagacgctgg gcaaaggaca gacagggctg gttaaactcg
     3541 gggtccactg catcacgggt cagaaggtcg ccatcaagat cgtgaaccgg gagaagctgt
     3601 cggagtcggt gctgatgaag gtggagcggg agatcgccat cctgaagctc atcgaacacc
     3661 cacatgtcct caagctccac gacgtctacg agaacaagaa atatttgtac ctggttctgg
     3721 agcacgtctc ggggggtgag ctattcgact acctggtaaa gaaggggaga ctgacgccca
     3781 aggaggcccg aaagttcttc cgccagattg tgtctgcgct ggacttctgc cacagctact
     3841 ccatctgcca cagagaccta aagcccgaga acctgctttt ggatgagaaa aacaacatcc
     3901 gcattgcaga cttcggcatg gcgtccctgc aggtggggga cagcctcctg gagaccagct
     3961 gcgggtcccc ccattatgcg tgtccagagg tgattaaggg ggaaaaatat gatggccgcc
     4021 gggcagacat gtggagctgt ggagtcatcc tcttcgccct gctcgtgggg gctctgccct
     4081 ttgatgacga caacctccgc cagctgctgg agaaggtgaa acgaggcgtc ttccacatgc
     4141 cccacttcat tcctccagat tgccagagcc tcctgagggg aatgatcgaa gtggagcccg
     4201 aaaaaaggct cagtctggag caaattcaga aacatccttg gtacctaggc gggaaacacg
     4261 agccagaccc gtgcctggag ccagcccctg gccgccgggt agccatgcgg agcctgccat
     4321 ccaacggaga gctggacccc gacgtcctag agagcatggc atcactgggc tgcttcaggg
     4381 accgcgagag gctgcatcgc gagctgcgca gtgaggagga gaaccaagaa aagatgatat
     4441 attatctgct tttggatcgg aaggagcggt atcccagctg tgaggaccag gacctgcctc
     4501 cccggaatga tgttgacccc ccccggaagc gtgtggattc tcccatgctg agccgtcacg
     4561 ggaagcggcg accagagcgg aagtccatgg aagtcctgag catcaccgat gccgggggtg
     4621 gtggctcccc tgtacccacc cgacgggcct tggagatggc ccagcacagc cagagatccc
     4681 gtagcgtcag tggagcctcc acgggtctgt cctccagccc tctaagcagc ccaaggagtc
     4741 cggtcttttc cttttcaccg gagccggggg ctggagatga ggctcgaggc gggggctccc
     4801 cgacttccaa aacgcagacg ctgccttctc ggggccccag gggtgggggc gccggggagc
     4861 agcccccgcc ccccagtgcc cgctccacac ccctgcccgg ccccccaggc tccccgcgct
     4921 cctctggcgg gacccccttg cactcgcctc tgcacacgcc ccgggccagt cccaccggga
     4981 ccccggggac aacaccaccc cccagccccg gcggtggcgt cgggggagcc gcctggagga
     5041 gtcgtctcaa ctccatccgc aacagcttcc tgggctcccc tcgctttcac cggcgcaaga
     5101 tgcaggtccc taccgctgag gagatgtcca gcttgacgcc agagtcctcc ccggagctgg
     5161 caaaacgctc ctggttcggg aacttcatct ccttggacaa agaagaacaa atattcctcg
     5221 tgctaaagga caaacctctc agcagcatca aagcagacat cgtccatgcc tttctgtcga
     5281 tccccagcct gagtcacagt gtgctgtcac agaccagctt cagggccgag tacaaggcca
     5341 gtggcggccc ctccgtcttc caaaagcccg tccgcttcca ggtggacatc agctcctctg
     5401 agggtccaga gccctccccg cgacgggacg gcagcggagg tggtggcatc tactccgtca
     5461 ccttcactct catctcgggt cccagccgtc ggttcaagcg agtggtggag accatccagg
     5521 cacagctcct gagcactcat gaccagccct ccgtgcaggc cctggcagac gagaagaacg
     5581 gggcccagac ccggcctgct ggtgccccac cccgaagcct gcagccccca cccggccgcc
     5641 cagacccaga gctgagcagc tctccccgcc gaggcccccc caaggacaag aagctcctgg
     5701 ccaccaacgg gacccctctg cccaacccag ctttcttgta caaagtgggc ggcaagccca
     5761 tccccaaccc cctgctgggc ctggacagca ccgctagcat cccgctgctg aacgctaaac
     5821 agattctggg acagctggac ggcgggagcg tggcagtcct gcctgtggtc gactccacca
     5881 atcagtacct gctggatcga atcggcgagc tgaagagtgg ggatgcttgc attgcagaat
     5941 atcagcaggc agggagagga agcagaggga ggaaatggtt ctctcctttt ggagctaacc
     6001 tgtacctgag tatgttttgg cgcctgaagc ggggaccagc agcaatcggc ctgggcccgg
     6061 tcatcggaat tgtcatggca gaagcgctgc gaaagctggg agcagacaag gtgcgagtca
     6121 aatggcccaa tgacctgtat ctgcaggata gaaagctggc aggcatcctg gtggagctgg
     6181 ccggaataac aggcgatgct gcacagatcg tcattggcgc cgggattaac gtggctatga
     6241 ggcgcgtgga ggaaagcgtg gtcaatcagg gctggatcac actgcaggaa gcagggatta
     6301 acctggacag gaatactctg gccgctatgc tgatccgaga gctgcgggca gccctggaac
     6361 tgttcgagca ggaaggcctg gctccatatc tgtcacggtg ggagaagctg gataacttca
     6421 tcaatagacc cgtgaagctg atcattgggg acaaagagat tttcgggatt agccggggga
     6481 ttgataaaca gggagccctg ctgctggaac aggacggagt tatcaaaccc tggatgggcg
     6541 gagaaatcag tctgcggtct gccgaaaagt aagccaactt tattatacat agttgatatc
     6601 cagcacagtg gcggccgctc gacaatcaac ctctggatta caaaatttgt gaaagattga
     6661 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     6721 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     6781 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     6841 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     6901 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     6961 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     7021 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     7081 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     7141 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     7201 ccgcctcccc gcctggaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
     7261 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc
     7321 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg
     7381 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc
     7441 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct
     7501 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct
     7561 ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg
     7621 ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc
     7681 acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc
     7741 ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     7801 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     7861 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     7921 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     7981 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     8041 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     8101 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     8161 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     8221 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     8281 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     8341 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat
     8401 ctcgagggcc cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     8461 tttttaaaag aaaagggggg actggaaggg ctagctcact cccaacgaag acaagatctg
     8521 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     8581 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     8641 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     8701 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     8761 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     8821 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     8881 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     8941 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     9001 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     9061 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     9121 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     9181 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     9241 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     9301 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     9361 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     9421 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     9481 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     9541 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     9601 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     9661 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     9721 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     9781 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     9841 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     9901 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     9961 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
    10021 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
    10081 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
    10141 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
    10201 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
    10261 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
    10321 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
    10381 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
    10441 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
    10501 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
    10561 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
    10621 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
    10681 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
    10741 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
    10801 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
    10861 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
    10921 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
    10981 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    11041 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    11101 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    11161 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    11221 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    11281 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    11341 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    11401 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    11461 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    11521 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    11581 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
    11641 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
    11701 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
    11761 gcgggcagtg agcgcaacgc aat
//
