LOCUS       sequence-269110-       11492 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of BRSK2 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11492)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-BRSK2_IDG-K
FEATURES             Location/Qualifiers
     source          1..11492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1983..2099
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2133..2153
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2153..3364
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3201..3220
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(3368..3388)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      3389..3398
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3401..4171
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             4178..4219
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    4220..4244
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6279..6303)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6333..6921
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6386..6406)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6804..6815)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6935..7434
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6970..6993)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7367..7386
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7455..8054
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7455..7474)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7951..7971
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8192..8425
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8497..8618
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8534..8553)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8588..8607
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8658..8793
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8813..8832)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8814..8832)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8842..8859)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8842..8858)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8851..8873)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9000..9455
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9087..9106)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9297..9318
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9481..9585
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9586..9654,9655..10446)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10617..11205
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11106..11125
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11359..11376
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta
     2101 tcgtccagtg tggtggaatt ctgcagatat cacaactttg tatagaaaag ttggcctccg
     2161 cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca
     2221 gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac agcggcccgc
     2281 tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt aggacgggac
     2341 ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga ggaaaagtag
     2401 tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg atgattatat
     2461 aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc
     2521 ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg
     2581 ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc gccaagggct
     2641 gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag cgcagcaaaa
     2701 tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg
     2761 aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg
     2821 cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa
     2881 gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca gttatggcgg
     2941 tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc
     3001 acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc
     3061 ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc
     3121 gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg
     3181 tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag
     3241 tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa
     3301 ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg cttttttgtt
     3361 agacggccaa cttttgtata caaagtgggc caccatggga atcccgctgc tgaacgctaa
     3421 acagattctg ggacagctgg acggcgggag cgtggcagtc ctgcctgtgg tcgactccac
     3481 caatcagtac ctgctggatc gaatcggcga gctgaagagt ggggatgctt gcattgcaga
     3541 atatcagcag gcagggagag gaagcagagg gaggaaatgg ttctctcctt ttggagctaa
     3601 cctgtacctg agtatgtttt ggcgcctgaa gcggggacca gcagcaatcg gcctgggccc
     3661 ggtcatcgga attgtcatgg cagaagcgct gcgaaagctg ggagcagaca aggtgcgagt
     3721 caaatggccc aatgacctgt atctgcagga tagaaagctg gcaggcatcc tggtggagct
     3781 ggccggaata acaggcgatg ctgcacagat cgtcattggc gccgggatta acgtggctat
     3841 gaggcgcgtg gaggaaagcg tggtcaatca gggctggatc acactgcagg aagcagggat
     3901 taacctggac aggaatactc tggccgctat gctgatccga gagctgcggg cagccctgga
     3961 actgttcgag caggaaggcc tggctccata tctgtcacgg tgggagaagc tggataactt
     4021 catcaataga cccgtgaagc tgatcattgg ggacaaagag attttcggga ttagccgggg
     4081 gattgataaa cagggagccc tgctgctgga acaggacgga gttatcaaac cctggatggg
     4141 cggagaaatc agtctgcggt ctgccgaaaa ggctagcggc aagcccatcc ccaaccccct
     4201 gctgggcctg gacagcacca caactttgta caaaaaagca gaagggccgt caaggcccac
     4261 catgacatcg acggggaagg acggcggcgc gcagcacgcg cagtatgttg ggccctaccg
     4321 gctggagaag acgctgggca aggggcagac aggtctggtg aagctggggg ttcactgcgt
     4381 cacctgccag aaggtggcca tcaagatcgt caaccgtgag aagctcagcg agtcggtgct
     4441 gatgaaggtg gagcgggaga tcgcgatcct gaagctcatt gagcaccccc acgtcctaaa
     4501 gctgcacgac gtttatgaaa acaaaaaata tttgtacctg gtgctagaac acgtgtcagg
     4561 tggtgagctc ttcgactacc tggtgaagaa ggggaggctg acgcctaagg aggctcggaa
     4621 gttcttccgg cagatcatct ctgcgctgga cttctgccac agccactcca tatgccacag
     4681 ggatctgaaa cctgaaaacc tcctgctgga cgagaagaac aacatccgca tcgcagactt
     4741 tggcatggcg tccctgcagg ttggcgacag cctgttggag accagctgtg ggtcccccca
     4801 ctacgcctgc cccgaggtga tccgggggga gaagtatgac ggccggaagg cggacgtgtg
     4861 gagctgcggc gtcatcctgt tcgccttgct ggtgggggct ctgcccttcg acgatgacaa
     4921 cttgcgacag ctgctggaga aggtgaagcg gggcgtgttc cacatgccgc actttatccc
     4981 gcccgactgc cagagtctgc tacggggcat gatcgaggtg gacgccgcac gccgcctcac
     5041 gctagagcac attcagaaac acatatggta tatagggggc aagaatgagc ccgaaccaga
     5101 gcagcccatt cctcgcaagg tgcagatccg ctcgctgccc agcctggagg acatcgaccc
     5161 cgacgtgctg gacagcatgc actcactggg ctgcttccga gaccgcaaca agctgctgca
     5221 ggacctgctg tccgaggagg agaaccagga gaagatgatt tacttcctcc tcctggaccg
     5281 gaaagaaagg tacccgagcc aggaggatga ggacctgccc ccccggaacg agatagaccc
     5341 tccccggaag cgtgtggact ccccgatgct gaaccggcac ggcaagcggc ggccagaacg
     5401 caaatccatg gaggtgctca gcgtgacgga cggcggctcc ccggtgcctg cgcggcgggc
     5461 cattgagatg gcccagcacg gccagaggtc tcggtccatc agcggtgcct cctcaggcct
     5521 ttccaccagc ccactcagca gcccccgggt gacccctcac ccctcaccaa ggggcagtcc
     5581 cctccccacc cccaagggga cacctgtcca cacgccaaag gagagcccgg ctggcacgcc
     5641 caaccccacg cccccgtcca gccccagcgt cggaggggtg ccctggaggg cgcggctcaa
     5701 ctccatcaag aacagctttc tgggctcacc ccgcttccac cgccggaaac tgcaagttcc
     5761 gacgccggag gagatgtcca acctgacacc agagtcgtcc ccagagctgg cgaagaagtc
     5821 ctggtttggg aacttcatca gcctggagaa ggaggagcag atcttcgtgg tcatcaaaga
     5881 caaacctctg agctccatca aggctgacat cgtgcacgcc ttcctgtcga ttcccagtct
     5941 cagccacagc gtcatctccc aaacgagctt ccgggccgag tacaaggcca cgggggggcc
     6001 agccgtgttc cagaagccgg tcaagttcca ggttgatatc acctacacgg agggtgggga
     6061 ggcgcagaag gagaacggca tctactccgt caccttcacc ctgctctcag gccccagccg
     6121 tcgcttcaag agggtggtgg agaccatcca ggcccagctg ctgagcacac acgacccgcc
     6181 tgcggcccag cacttgtcag acaccactaa ctgtatggaa atgatgacgg ggcggctttc
     6241 caaatgtgga attatcccga aaagttgagg cctcatgggc ccagctttct tgtacaaagt
     6301 tgtgatatcc agcacagtgg cggccgctcg acaatcaacc tctggattac aaaatttgtg
     6361 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     6421 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     6481 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     6541 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     6601 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     6661 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     6721 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     6781 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     6841 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     6901 ccctttgggc cgcctccccg cctggaattc taccgggtag gggaggcgct tttcccaagg
     6961 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
     7021 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
     7081 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
     7141 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
     7201 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
     7261 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
     7321 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
     7381 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
     7441 agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     7501 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     7561 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     7621 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     7681 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     7741 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     7801 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     7861 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     7921 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     7981 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     8041 agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac
     8101 cccatgcatc tcgagggccc ggtaccttta agaccaatga cttacaaggc agctgtagat
     8161 cttagccact ttttaaaaga aaagggggga ctggaagggc tagctcactc ccaacgaaga
     8221 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     8281 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     8341 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     8401 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     8461 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     8521 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     8581 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     8641 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     8701 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     8761 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     8821 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     8881 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     8941 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     9001 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     9061 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     9121 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     9181 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     9241 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     9301 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     9361 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     9421 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     9481 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     9541 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     9601 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     9661 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     9721 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     9781 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     9841 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     9901 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     9961 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
    10021 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
    10081 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
    10141 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
    10201 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
    10261 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
    10321 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
    10381 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
    10441 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
    10501 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
    10561 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
    10621 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
    10681 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
    10741 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
    10801 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
    10861 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
    10921 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
    10981 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
    11041 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
    11101 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
    11161 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
    11221 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
    11281 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
    11341 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
    11401 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
    11461 gactggaaag cgggcagtga gcgcaacgca at
//
