LOCUS       sequence-269111-       11798 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of BRSK1 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11798)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-BRSK1_IDG-K
FEATURES             Location/Qualifiers
     source          1..11798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1983..2099
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2133..2153
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2153..3364
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3201..3220
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(3368..3388)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      3389..3398
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3401..4171
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             4178..4219
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    4220..4244
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6585..6609)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6639..7227
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6692..6712)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7110..7121)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7241..7740
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7276..7299)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7673..7692
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7761..8360
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7761..7780)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8257..8277
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8498..8731
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8803..8924
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8840..8859)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8894..8913
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8964..9099
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9119..9138)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9120..9138)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9148..9165)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9148..9164)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9157..9179)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9306..9761
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9393..9412)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9603..9624
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9787..9891
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9892..9960,9961..10752)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10923..11511
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11412..11431
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11665..11682
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta
     2101 tcgtccagtg tggtggaatt ctgcagatat cacaactttg tatagaaaag ttggcctccg
     2161 cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca
     2221 gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac agcggcccgc
     2281 tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt aggacgggac
     2341 ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga ggaaaagtag
     2401 tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg atgattatat
     2461 aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc
     2521 ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg
     2581 ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc gccaagggct
     2641 gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag cgcagcaaaa
     2701 tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg
     2761 aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg
     2821 cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa
     2881 gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca gttatggcgg
     2941 tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc
     3001 acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc
     3061 ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc
     3121 gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg
     3181 tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag
     3241 tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa
     3301 ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg cttttttgtt
     3361 agacggccaa cttttgtata caaagtgggc caccatggga atcccgctgc tgaacgctaa
     3421 acagattctg ggacagctgg acggcgggag cgtggcagtc ctgcctgtgg tcgactccac
     3481 caatcagtac ctgctggatc gaatcggcga gctgaagagt ggggatgctt gcattgcaga
     3541 atatcagcag gcagggagag gaagcagagg gaggaaatgg ttctctcctt ttggagctaa
     3601 cctgtacctg agtatgtttt ggcgcctgaa gcggggacca gcagcaatcg gcctgggccc
     3661 ggtcatcgga attgtcatgg cagaagcgct gcgaaagctg ggagcagaca aggtgcgagt
     3721 caaatggccc aatgacctgt atctgcagga tagaaagctg gcaggcatcc tggtggagct
     3781 ggccggaata acaggcgatg ctgcacagat cgtcattggc gccgggatta acgtggctat
     3841 gaggcgcgtg gaggaaagcg tggtcaatca gggctggatc acactgcagg aagcagggat
     3901 taacctggac aggaatactc tggccgctat gctgatccga gagctgcggg cagccctgga
     3961 actgttcgag caggaaggcc tggctccata tctgtcacgg tgggagaagc tggataactt
     4021 catcaataga cccgtgaagc tgatcattgg ggacaaagag attttcggga ttagccgggg
     4081 gattgataaa cagggagccc tgctgctgga acaggacgga gttatcaaac cctggatggg
     4141 cggagaaatc agtctgcggt ctgccgaaaa ggctagcggc aagcccatcc ccaaccccct
     4201 gctgggcctg gacagcacca caactttgta caaaaaagca ggcttaatgt cgtccggggc
     4261 caaggaggga ggtgggggct ctcccgccta ccacctcccc cacccccacc cccacccacc
     4321 ccagcacgcc caatatgtgg gcccctatcg gctggagaag acgctgggca aaggacagac
     4381 agggctggtt aaactcgggg tccactgcat cacgggtcag aaggtcgcca tcaagatcgt
     4441 gaaccgggag aagctgtcgg agtcggtgct gatgaaggtg gagcgggaga tcgccatcct
     4501 gaagctcatc gaacacccac atgtcctcaa gctccacgac gtctacgaga acaagaaata
     4561 tttgtacctg gttctggagc acgtctcggg gggtgagcta ttcgactacc tggtaaagaa
     4621 ggggagactg acgcccaagg aggcccgaaa gttcttccgc cagattgtgt ctgcgctgga
     4681 cttctgccac agctactcca tctgccacag agacctaaag cccgagaacc tgcttttgga
     4741 tgagaaaaac aacatccgca ttgcagactt cggcatggcg tccctgcagg tgggggacag
     4801 cctcctggag accagctgcg ggtcccccca ttatgcgtgt ccagaggtga ttaaggggga
     4861 aaaatatgat ggccgccggg cagacatgtg gagctgtgga gtcatcctct tcgccctgct
     4921 cgtgggggct ctgccctttg atgacgacaa cctccgccag ctgctggaga aggtgaaacg
     4981 aggcgtcttc cacatgcccc acttcattcc tccagattgc cagagcctcc tgaggggaat
     5041 gatcgaagtg gagcccgaaa aaaggctcag tctggagcaa attcagaaac atccttggta
     5101 cctaggcggg aaacacgagc cagacccgtg cctggagcca gcccctggcc gccgggtagc
     5161 catgcggagc ctgccatcca acggagagct ggaccccgac gtcctagaga gcatggcatc
     5221 actgggctgc ttcagggacc gcgagaggct gcatcgcgag ctgcgcagtg aggaggagaa
     5281 ccaagaaaag atgatatatt atctgctttt ggatcggaag gagcggtatc ccagctgtga
     5341 ggaccaggac ctgcctcccc ggaatgatgt tgaccccccc cggaagcgtg tggattctcc
     5401 catgctgagc cgtcacggga agcggcgacc agagcggaag tccatggaag tcctgagcat
     5461 caccgatgcc gggggtggtg gctcccctgt acccacccga cgggccttgg agatggccca
     5521 gcacagccag agatcccgta gcgtcagtgg agcctccacg ggtctgtcct ccagccctct
     5581 aagcagccca aggagtccgg tcttttcctt ttcaccggag ccgggggctg gagatgaggc
     5641 tcgaggcggg ggctccccga cttccaaaac gcagacgctg ccttctcggg gccccagggg
     5701 tgggggcgcc ggggagcagc ccccgccccc cagtgcccgc tccacacccc tgcccggccc
     5761 cccaggctcc ccgcgctcct ctggcgggac ccccttgcac tcgcctctgc acacgccccg
     5821 ggccagtccc accgggaccc cggggacaac accacccccc agccccggcg gtggcgtcgg
     5881 gggagccgcc tggaggagtc gtctcaactc catccgcaac agcttcctgg gctcccctcg
     5941 ctttcaccgg cgcaagatgc aggtccctac cgctgaggag atgtccagct tgacgccaga
     6001 gtcctccccg gagctggcaa aacgctcctg gttcgggaac ttcatctcct tggacaaaga
     6061 agaacaaata ttcctcgtgc taaaggacaa acctctcagc agcatcaaag cagacatcgt
     6121 ccatgccttt ctgtcgatcc ccagcctgag tcacagtgtg ctgtcacaga ccagcttcag
     6181 ggccgagtac aaggccagtg gcggcccctc cgtcttccaa aagcccgtcc gcttccaggt
     6241 ggacatcagc tcctctgagg gtccagagcc ctccccgcga cgggacggca gcggaggtgg
     6301 tggcatctac tccgtcacct tcactctcat ctcgggtccc agccgtcggt tcaagcgagt
     6361 ggtggagacc atccaggcac agctcctgag cactcatgac cagccctccg tgcaggccct
     6421 ggcagacgag aagaacgggg cccagacccg gcctgctggt gccccacccc gaagcctgca
     6481 gcccccaccc ggccgcccag acccagagct gagcagctct ccccgccgag gcccccccaa
     6541 ggacaagaag ctcctggcca ccaacgggac ccctctgccc tgaaacccag ctttcttgta
     6601 caaagttgtg atatccagca cagtggcggc cgctcgacaa tcaacctctg gattacaaaa
     6661 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     6721 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     6781 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     6841 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     6901 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     6961 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     7021 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     7081 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     7141 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     7201 ggatctccct ttgggccgcc tccccgcctg gaattctacc gggtagggga ggcgcttttc
     7261 ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt
     7321 ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt
     7381 ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg
     7441 cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag
     7501 atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag
     7561 ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc
     7621 aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc
     7681 acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg
     7741 acctgcagcc caagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     7801 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     7861 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     7921 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     7981 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     8041 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     8101 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     8161 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     8221 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     8281 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     8341 cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg
     8401 cacgacccca tgcatctcga gggcccggta cctttaagac caatgactta caaggcagct
     8461 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctagc tcactcccaa
     8521 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     8581 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     8641 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     8701 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     8761 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     8821 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     8881 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     8941 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     9001 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     9061 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc
     9121 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     9181 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     9241 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     9301 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     9361 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     9421 cgccacgttc gccggctttc cccgtcaagc tctaaattgg gggctccctt tagggttccg
     9481 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     9541 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     9601 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     9661 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     9721 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     9781 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     9841 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     9901 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     9961 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
    10021 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
    10081 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
    10141 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
    10201 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
    10261 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
    10321 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
    10381 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
    10441 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
    10501 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
    10561 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
    10621 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
    10681 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
    10741 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
    10801 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
    10861 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    10921 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
    10981 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
    11041 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
    11101 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    11161 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    11221 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    11281 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    11341 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    11401 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    11461 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    11521 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
    11581 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
    11641 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
    11701 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
    11761 tttcccgact ggaaagcggg cagtgagcgc aacgcaat
//
