LOCUS       sequence-269112-       11678 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of BRSK2 (human) tagged with N-terminal 
            TurboID-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11678)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-TurboID-V5-BRSK2_IDG-K
FEATURES             Location/Qualifiers
     source          1..11678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1983..2099
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2133..2153
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2153..3364
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3201..3220
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(3368..3388)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      3389..3398
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3395..4357
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="MGKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKS
                     GDACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             4364..4405
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    4406..4430
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6465..6489)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6519..7107
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6572..6592)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6990..7001)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7121..7620
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7156..7179)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7553..7572
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7641..8240
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7641..7660)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8137..8157
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8378..8611
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8683..8804
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8720..8739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8774..8793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8844..8979
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8999..9018)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9000..9018)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9028..9045)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9028..9044)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9037..9059)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9186..9641
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9273..9292)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9483..9504
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9667..9771
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9772..9840,9841..10632)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10803..11391
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11292..11311
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11545..11562
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta
     2101 tcgtccagtg tggtggaatt ctgcagatat cacaactttg tatagaaaag ttggcctccg
     2161 cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca
     2221 gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac agcggcccgc
     2281 tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt aggacgggac
     2341 ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga ggaaaagtag
     2401 tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg atgattatat
     2461 aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc
     2521 ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg
     2581 ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc gccaagggct
     2641 gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag cgcagcaaaa
     2701 tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg
     2761 aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg
     2821 cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa
     2881 gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca gttatggcgg
     2941 tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc
     3001 acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc
     3061 ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc
     3121 gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg
     3181 tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag
     3241 tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa
     3301 ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg cttttttgtt
     3361 agacggccaa cttttgtata caaagtgggc caccatggga aaagacaata ctgtgcctct
     3421 gaagctgatc gctctcctgg ctaatggcga gttccatagt ggcgaacagc tgggagaaac
     3481 cctgggcatg tccagggccg ctatcaacaa gcacattcag actctgcgcg actggggcgt
     3541 ggacgtgttc accgtgcccg gaaagggcta ctctctgccc gagcctatcc cgctgctgaa
     3601 cgctaaacag attctgggac agctggacgg cgggagcgtg gcagtcctgc ctgtggtcga
     3661 ctccaccaat cagtacctgc tggatcgaat cggcgagctg aagagtgggg atgcttgcat
     3721 tgcagaatat cagcaggcag ggagaggaag cagagggagg aaatggttct ctccttttgg
     3781 agctaacctg tacctgagta tgttttggcg cctgaagcgg ggaccagcag caatcggcct
     3841 gggcccggtc atcggaattg tcatggcaga agcgctgcga aagctgggag cagacaaggt
     3901 gcgagtcaaa tggcccaatg acctgtatct gcaggataga aagctggcag gcatcctggt
     3961 ggagctggcc ggaataacag gcgatgctgc acagatcgtc attggcgccg ggattaacgt
     4021 ggctatgagg cgcgtggagg aaagcgtggt caatcagggc tggatcacac tgcaggaagc
     4081 agggattaac ctggacagga atactctggc cgctacgctg atccgagagc tgcgggcagc
     4141 cctggaactg ttcgagcagg aaggcctggc tccatatctg ccacggtggg agaagctgga
     4201 taacttcatc aatagacccg tgaagctgat cattggggac aaagagattt tcgggattag
     4261 ccgggggatt gataaacagg gagccctgct gctggaacag gacggagtta tcaaaccctg
     4321 gatgggcgga gaaatcagtc tgcggtctgc cgaaaaggct agcggcaagc ccatccccaa
     4381 ccccctgctg ggcctggaca gcaccacaac tttgtacaaa aaagcagaag ggccgtcaag
     4441 gcccaccatg acatcgacgg ggaaggacgg cggcgcgcag cacgcgcagt atgttgggcc
     4501 ctaccggctg gagaagacgc tgggcaaggg gcagacaggt ctggtgaagc tgggggttca
     4561 ctgcgtcacc tgccagaagg tggccatcaa gatcgtcaac cgtgagaagc tcagcgagtc
     4621 ggtgctgatg aaggtggagc gggagatcgc gatcctgaag ctcattgagc acccccacgt
     4681 cctaaagctg cacgacgttt atgaaaacaa aaaatatttg tacctggtgc tagaacacgt
     4741 gtcaggtggt gagctcttcg actacctggt gaagaagggg aggctgacgc ctaaggaggc
     4801 tcggaagttc ttccggcaga tcatctctgc gctggacttc tgccacagcc actccatatg
     4861 ccacagggat ctgaaacctg aaaacctcct gctggacgag aagaacaaca tccgcatcgc
     4921 agactttggc atggcgtccc tgcaggttgg cgacagcctg ttggagacca gctgtgggtc
     4981 cccccactac gcctgccccg aggtgatccg gggggagaag tatgacggcc ggaaggcgga
     5041 cgtgtggagc tgcggcgtca tcctgttcgc cttgctggtg ggggctctgc ccttcgacga
     5101 tgacaacttg cgacagctgc tggagaaggt gaagcggggc gtgttccaca tgccgcactt
     5161 tatcccgccc gactgccaga gtctgctacg gggcatgatc gaggtggacg ccgcacgccg
     5221 cctcacgcta gagcacattc agaaacacat atggtatata gggggcaaga atgagcccga
     5281 accagagcag cccattcctc gcaaggtgca gatccgctcg ctgcccagcc tggaggacat
     5341 cgaccccgac gtgctggaca gcatgcactc actgggctgc ttccgagacc gcaacaagct
     5401 gctgcaggac ctgctgtccg aggaggagaa ccaggagaag atgatttact tcctcctcct
     5461 ggaccggaaa gaaaggtacc cgagccagga ggatgaggac ctgccccccc ggaacgagat
     5521 agaccctccc cggaagcgtg tggactcccc gatgctgaac cggcacggca agcggcggcc
     5581 agaacgcaaa tccatggagg tgctcagcgt gacggacggc ggctccccgg tgcctgcgcg
     5641 gcgggccatt gagatggccc agcacggcca gaggtctcgg tccatcagcg gtgcctcctc
     5701 aggcctttcc accagcccac tcagcagccc ccgggtgacc cctcacccct caccaagggg
     5761 cagtcccctc cccaccccca aggggacacc tgtccacacg ccaaaggaga gcccggctgg
     5821 cacgcccaac cccacgcccc cgtccagccc cagcgtcgga ggggtgccct ggagggcgcg
     5881 gctcaactcc atcaagaaca gctttctggg ctcaccccgc ttccaccgcc ggaaactgca
     5941 agttccgacg ccggaggaga tgtccaacct gacaccagag tcgtccccag agctggcgaa
     6001 gaagtcctgg tttgggaact tcatcagcct ggagaaggag gagcagatct tcgtggtcat
     6061 caaagacaaa cctctgagct ccatcaaggc tgacatcgtg cacgccttcc tgtcgattcc
     6121 cagtctcagc cacagcgtca tctcccaaac gagcttccgg gccgagtaca aggccacggg
     6181 ggggccagcc gtgttccaga agccggtcaa gttccaggtt gatatcacct acacggaggg
     6241 tggggaggcg cagaaggaga acggcatcta ctccgtcacc ttcaccctgc tctcaggccc
     6301 cagccgtcgc ttcaagaggg tggtggagac catccaggcc cagctgctga gcacacacga
     6361 cccgcctgcg gcccagcact tgtcagacac cactaactgt atggaaatga tgacggggcg
     6421 gctttccaaa tgtggaatta tcccgaaaag ttgaggcctc atgggcccag ctttcttgta
     6481 caaagttgtg atatccagca cagtggcggc cgctcgacaa tcaacctctg gattacaaaa
     6541 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     6601 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     6661 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     6721 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     6781 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     6841 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     6901 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     6961 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     7021 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     7081 ggatctccct ttgggccgcc tccccgcctg gaattctacc gggtagggga ggcgcttttc
     7141 ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt
     7201 ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt
     7261 ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg
     7321 cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag
     7381 atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag
     7441 ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc
     7501 aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc
     7561 acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg
     7621 acctgcagcc caagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     7681 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     7741 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     7801 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     7861 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     7921 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     7981 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     8041 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     8101 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     8161 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     8221 cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg
     8281 cacgacccca tgcatctcga gggcccggta cctttaagac caatgactta caaggcagct
     8341 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctagc tcactcccaa
     8401 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     8461 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     8521 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     8581 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     8641 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     8701 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     8761 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     8821 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     8881 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     8941 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc
     9001 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     9061 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     9121 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     9181 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     9241 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     9301 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     9361 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     9421 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     9481 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     9541 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     9601 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     9661 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     9721 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     9781 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     9841 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     9901 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     9961 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
    10021 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
    10081 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
    10141 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
    10201 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
    10261 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
    10321 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
    10381 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
    10441 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
    10501 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
    10561 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
    10621 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
    10681 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
    10741 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    10801 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
    10861 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
    10921 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
    10981 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    11041 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    11101 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    11161 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    11221 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    11281 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    11341 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    11401 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
    11461 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
    11521 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
    11581 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
    11641 tttcccgact ggaaagcggg cagtgagcgc aacgcaat
//
