LOCUS       sequence-269116-       11945 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of TLK2 (human) tagged with C-terminal 
            V5-TurboID for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11945)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-TLK2-V5-TurboID_IDG-K
FEATURES             Location/Qualifiers
     source          1..11945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1983..2099
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2131..2151
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2151..3362
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3199..3218
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    3363..3387
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(3965..3976)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(5701..5725)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5727..5768
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5775..6731
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(6737..6757)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    6786..7374
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6839..6859)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7257..7268)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7388..7887
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7423..7446)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7820..7839
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7908..8507
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7908..7927)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8404..8424
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8645..8878
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8950..9071
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8987..9006)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9041..9060
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9111..9246
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9266..9285)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9267..9285)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9295..9312)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9295..9311)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9304..9326)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9453..9908
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9540..9559)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9750..9771
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9934..10038
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10039..10107,10108..10899)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11070..11658
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11559..11578
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11812..11829
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta
     2101 tcgtccagtg tggtggaatt ctgcagatat caactttgta tagaaaagtt ggcctccgcg
     2161 ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga
     2221 cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg
     2281 ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt
     2341 gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc
     2401 ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa
     2461 ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt
     2521 gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct
     2581 ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt
     2641 agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg
     2701 gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa
     2761 acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg
     2821 ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt
     2881 ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg
     2941 ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac
     3001 ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt
     3061 ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc
     3121 cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta
     3181 cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg
     3241 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt
     3301 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag
     3361 acgcaacttt gtacaaaaaa gttggcatgg aagaattgca tagcctggac ccacgacggc
     3421 aggaattatt ggaggccagg tttactggag taggtgttag taagggacca cttaatagtg
     3481 agtcttccaa ccagagcttg tgcagcgtcg gatccttgag tgataaagaa gtagagactc
     3541 ccgagaaaaa gcagaatgac cagcgaaatc ggaaaagaaa agctgaacca tatgaaacta
     3601 gccaagggaa aggcactcct aggggacata aaattagtga ttactttgag tttgctgggg
     3661 gaagcgcgcc aggaaccagc cctggcagaa gtgttccacc agttgcacga tcctcaccgc
     3721 aacattcctt atccaatccc ttaccgcgac gagtagaaca gcccctctat ggtttagatg
     3781 gcagtgctgc aaaggaggca acggaggagc agtctgctct gccaaccctc atgtcagtga
     3841 tgctagcaaa acctcggctt gacacagagc agctggcgca aaggggagct ggcctctgct
     3901 tcacttttgt ttcagctcag caaaacagtc cctcatctac gggatctggc aacacagagc
     3961 attcctgcag ctcccaaaaa cagatctcca tccagcacag acagacccag tccgacctca
     4021 caatagaaaa aatatctgca ctagaaaaca gtaagaattc tgacttagag aagaaggagg
     4081 gaagaataga tgatttatta agagccaact gtgatttgag acggcagatt gatgaacagc
     4141 aaaagatgct agagaaatac aaggaacgat taaatagatg tgtgacaatg agcaagaaac
     4201 tccttataga aaagtcaaaa caagagaaga tggcgtgtag agataagagc atgcaagacc
     4261 gcttgagact gggccacttt actactgtcc gacacggagc ctcatttact gaacagtgga
     4321 cagatggtta tgcttttcag aatcttatca agcaacagga aaggataaat tcacagaggg
     4381 aagagataga aagacaacgg aaaatgttag caaagcggaa acctcctgcc atgggtcagg
     4441 cccctcctgc aaccaatgag cagaaacagc ggaaaagcaa gaccaatgga gctgaaaatg
     4501 aaacgccctc ttctgggaat acagagctaa aggatacagc cccagcctta ggagcccaca
     4561 gtttacttag gttaacgtta gcagaatacc atgaacaaga agaaatcttc aaactcagat
     4621 taggtcatct taaaaaggag gaagcagaga tccaggcaga gctggagaga ctagaaaggg
     4681 ttagaaatct acatatcagg gaactaaaaa ggatacataa tgaagataat tcacaattta
     4741 aagatcatcc aacgctaaat gacagatatt tgttgttaca tcttttgggt agaggaggtt
     4801 tcagtgaagt ttacaaggca tttgatctaa cagagcaaag atacgtagct gtgaaaattc
     4861 accagttaaa taaaaactgg agagatgaga aaaaggagaa ttaccacaag catgcatgta
     4921 gggaataccg gattcataaa gagctggatc atcccagaat agttaagctg tatgattact
     4981 tttcactgga tactgactcg ttttgtacag tattagaata ctgtgaggga aatgatctgg
     5041 acttctacct gaaacagcac aaattaatgt cggagaaaga ggcccggtcc attatcatgc
     5101 agattgtgaa tgctttaaag tacttaaatg aaataaaacc tcccatcata cactatgacc
     5161 tcaaaccagg taatattctt ttagtaaatg gtacagcgtg tggagagata aaaattacag
     5221 attttggtct ttcgaagatc atggatgatg atagctacaa ttcagtggat ggcatggagc
     5281 taacatcaca aggtgctggt acttattggt atttaccacc agagtgtttt gtggttggga
     5341 aagaaccacc aaagatctca aataaagttg atgtgtggtc ggtgggtgtg atcttctatc
     5401 agtgtcttta tggaaggaag ccttttggcc ataaccagtc tcagcaagac atcctacaag
     5461 agaatacgat tcttaaagct actgaagtgc agttcccgcc aaagccagta gtaacacctg
     5521 aagcaaaggc gtttattcga cgatgcttgg cctaccgaaa ggaggaccgc attgatgtcc
     5581 agcagctggc ctgtgatccc tacttgttgc ctcacatccg aaagtcagtc tctacaagta
     5641 gccctgctgg agctgctatt gcatcaacct ctggggcgtc caataacagt tcttctaatt
     5701 gcccaacttt cttgtacaaa gtgggcggca agcccatccc caaccccctg ctgggcctgg
     5761 acagcaccgc tagcaaagac aatactgtgc ctctgaagct gatcgctctc ctggctaatg
     5821 gcgagttcca tagtggcgaa cagctgggag aaaccctggg catgtccagg gccgctatca
     5881 acaagcacat tcagactctg cgcgactggg gcgtggacgt gttcaccgtg cccggaaagg
     5941 gctactctct gcccgagcct atcccgctgc tgaacgctaa acagattctg ggacagctgg
     6001 acggcgggag cgtggcagtc ctgcctgtgg tcgactccac caatcagtac ctgctggatc
     6061 gaatcggcga gctgaagagt ggggatgctt gcattgcaga atatcagcag gcagggagag
     6121 gaagcagagg gaggaaatgg ttctctcctt ttggagctaa cctgtacctg agtatgtttt
     6181 ggcgcctgaa gcggggacca gcagcaatcg gcctgggccc ggtcatcgga attgtcatgg
     6241 cagaagcgct gcgaaagctg ggagcagaca aggtgcgagt caaatggccc aatgacctgt
     6301 atctgcagga tagaaagctg gcaggcatcc tggtggagct ggccggaata acaggcgatg
     6361 ctgcacagat cgtcattggc gccgggatta acgtggctat gaggcgcgtg gaggaaagcg
     6421 tggtcaatca gggctggatc acactgcagg aagcagggat taacctggac aggaatactc
     6481 tggccgctac gctgatccga gagctgcggg cagccctgga actgttcgag caggaaggcc
     6541 tggctccata tctgccacgg tgggagaagc tggataactt catcaataga cccgtgaagc
     6601 tgatcattgg ggacaaagag attttcggga ttagccgggg gattgataaa cagggagccc
     6661 tgctgctgga acaggacgga gttatcaaac cctggatggg cggagaaatc agtctgcggt
     6721 ctgccgaaaa gtaagccaac tttattatac atagttgata tccagcacag tggcggccgc
     6781 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     6841 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     6901 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     6961 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     7021 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     7081 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     7141 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     7201 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     7261 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     7321 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     7381 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     7441 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     7501 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     7561 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     7621 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     7681 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     7741 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
     7801 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
     7861 tcctcttcct catctccggg cctttcgacc tgcagcccaa gcttaccatg accgagtaca
     7921 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     7981 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     8041 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     8101 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     8161 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     8221 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     8281 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     8341 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     8401 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     8461 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg
     8521 acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atctcgaggg cccggtacct
     8581 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     8641 ggactggaag ggctagctca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     8701 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     8761 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     8821 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     8881 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     8941 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     9001 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     9061 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     9121 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     9181 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     9241 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     9301 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     9361 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     9421 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     9481 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     9541 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     9601 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     9661 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     9721 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     9781 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     9841 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     9901 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     9961 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
    10021 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
    10081 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
    10141 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
    10201 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
    10261 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
    10321 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
    10381 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
    10441 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
    10501 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
    10561 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
    10621 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
    10681 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
    10741 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
    10801 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
    10861 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
    10921 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
    10981 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
    11041 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
    11101 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
    11161 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
    11221 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
    11281 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
    11341 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
    11401 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
    11461 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
    11521 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
    11581 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
    11641 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
    11701 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
    11761 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
    11821 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
    11881 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
    11941 gcaat
//
