LOCUS       sequence-269118-       11480 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of BRSK2 (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11480)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-BRSK2-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..11480
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1983..2099
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2131..2151
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2151..3362
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3199..3218
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    3363..3387
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5422..5446)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5448..5489
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5496..6266
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(6272..6292)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    6321..6909
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6374..6394)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6792..6803)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6923..7422
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6958..6981)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7355..7374
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7443..8042
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7443..7462)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7939..7959
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8180..8413
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8485..8606
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8522..8541)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8576..8595
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8646..8781
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8801..8820)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8802..8820)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8830..8847)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8830..8846)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8839..8861)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8988..9443
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9075..9094)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9285..9306
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9469..9573
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9574..9642,9643..10434)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10605..11193
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11094..11113
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11347..11364
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta
     2101 tcgtccagtg tggtggaatt ctgcagatat caactttgta tagaaaagtt ggcctccgcg
     2161 ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga
     2221 cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg
     2281 ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt
     2341 gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc
     2401 ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa
     2461 ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt
     2521 gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct
     2581 ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt
     2641 agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg
     2701 gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa
     2761 acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg
     2821 ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt
     2881 ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg
     2941 ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac
     3001 ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt
     3061 ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc
     3121 cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta
     3181 cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg
     3241 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt
     3301 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag
     3361 acgcaacttt gtacaaaaaa gcagaagggc cgtcaaggcc caccatgaca tcgacgggga
     3421 aggacggcgg cgcgcagcac gcgcagtatg ttgggcccta ccggctggag aagacgctgg
     3481 gcaaggggca gacaggtctg gtgaagctgg gggttcactg cgtcacctgc cagaaggtgg
     3541 ccatcaagat cgtcaaccgt gagaagctca gcgagtcggt gctgatgaag gtggagcggg
     3601 agatcgcgat cctgaagctc attgagcacc cccacgtcct aaagctgcac gacgtttatg
     3661 aaaacaaaaa atatttgtac ctggtgctag aacacgtgtc aggtggtgag ctcttcgact
     3721 acctggtgaa gaaggggagg ctgacgccta aggaggctcg gaagttcttc cggcagatca
     3781 tctctgcgct ggacttctgc cacagccact ccatatgcca cagggatctg aaacctgaaa
     3841 acctcctgct ggacgagaag aacaacatcc gcatcgcaga ctttggcatg gcgtccctgc
     3901 aggttggcga cagcctgttg gagaccagct gtgggtcccc ccactacgcc tgccccgagg
     3961 tgatccgggg ggagaagtat gacggccgga aggcggacgt gtggagctgc ggcgtcatcc
     4021 tgttcgcctt gctggtgggg gctctgccct tcgacgatga caacttgcga cagctgctgg
     4081 agaaggtgaa gcggggcgtg ttccacatgc cgcactttat cccgcccgac tgccagagtc
     4141 tgctacgggg catgatcgag gtggacgccg cacgccgcct cacgctagag cacattcaga
     4201 aacacatatg gtatataggg ggcaagaatg agcccgaacc agagcagccc attcctcgca
     4261 aggtgcagat ccgctcgctg cccagcctgg aggacatcga ccccgacgtg ctggacagca
     4321 tgcactcact gggctgcttc cgagaccgca acaagctgct gcaggacctg ctgtccgagg
     4381 aggagaacca ggagaagatg atttacttcc tcctcctgga ccggaaagaa aggtacccga
     4441 gccaggagga tgaggacctg cccccccgga acgagataga ccctccccgg aagcgtgtgg
     4501 actccccgat gctgaaccgg cacggcaagc ggcggccaga acgcaaatcc atggaggtgc
     4561 tcagcgtgac ggacggcggc tccccggtgc ctgcgcggcg ggccattgag atggcccagc
     4621 acggccagag gtctcggtcc atcagcggtg cctcctcagg cctttccacc agcccactca
     4681 gcagcccccg ggtgacccct cacccctcac caaggggcag tcccctcccc acccccaagg
     4741 ggacacctgt ccacacgcca aaggagagcc cggctggcac gcccaacccc acgcccccgt
     4801 ccagccccag cgtcggaggg gtgccctgga gggcgcggct caactccatc aagaacagct
     4861 ttctgggctc accccgcttc caccgccgga aactgcaagt tccgacgccg gaggagatgt
     4921 ccaacctgac accagagtcg tccccagagc tggcgaagaa gtcctggttt gggaacttca
     4981 tcagcctgga gaaggaggag cagatcttcg tggtcatcaa agacaaacct ctgagctcca
     5041 tcaaggctga catcgtgcac gccttcctgt cgattcccag tctcagccac agcgtcatct
     5101 cccaaacgag cttccgggcc gagtacaagg ccacgggggg gccagccgtg ttccagaagc
     5161 cggtcaagtt ccaggttgat atcacctaca cggagggtgg ggaggcgcag aaggagaacg
     5221 gcatctactc cgtcaccttc accctgctct caggccccag ccgtcgcttc aagagggtgg
     5281 tggagaccat ccaggcccag ctgctgagca cacacgaccc gcctgcggcc cagcacttgt
     5341 cagacaccac taactgtatg gaaatgatga cggggcggct ttccaaatgt ggaattatcc
     5401 cgaaaagttc aggcctcatg ggcccagctt tcttgtacaa agtgggcggc aagcccatcc
     5461 ccaaccccct gctgggcctg gacagcaccg ctagcatccc gctgctgaac gctaaacaga
     5521 ttctgggaca gctggacggc gggagcgtgg cagtcctgcc tgtggtcgac tccaccaatc
     5581 agtacctgct ggatcgaatc ggcgagctga agagtgggga tgcttgcatt gcagaatatc
     5641 agcaggcagg gagaggaagc agagggagga aatggttctc tccttttgga gctaacctgt
     5701 acctgagtat gttttggcgc ctgaagcggg gaccagcagc aatcggcctg ggcccggtca
     5761 tcggaattgt catggcagaa gcgctgcgaa agctgggagc agacaaggtg cgagtcaaat
     5821 ggcccaatga cctgtatctg caggatagaa agctggcagg catcctggtg gagctggccg
     5881 gaataacagg cgatgctgca cagatcgtca ttggcgccgg gattaacgtg gctatgaggc
     5941 gcgtggagga aagcgtggtc aatcagggct ggatcacact gcaggaagca gggattaacc
     6001 tggacaggaa tactctggcc gctatgctga tccgagagct gcgggcagcc ctggaactgt
     6061 tcgagcagga aggcctggct ccatatctgt cacggtggga gaagctggat aacttcatca
     6121 atagacccgt gaagctgatc attggggaca aagagatttt cgggattagc cgggggattg
     6181 ataaacaggg agccctgctg ctggaacagg acggagttat caaaccctgg atgggcggag
     6241 aaatcagtct gcggtctgcc gaaaagtaag ccaactttat tatacatagt tgatatccag
     6301 cacagtggcg gccgctcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     6361 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     6421 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     6481 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     6541 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     6601 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     6661 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     6721 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     6781 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     6841 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     6901 cctccccgcc tggaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
     6961 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
     7021 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
     7081 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
     7141 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
     7201 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
     7261 tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc
     7321 ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg
     7381 tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta
     7441 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     7501 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     7561 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     7621 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     7681 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     7741 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     7801 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     7861 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     7921 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     7981 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     8041 gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatctc
     8101 gagggcccgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     8161 ttaaaagaaa aggggggact ggaagggcta gctcactccc aacgaagaca agatctgctt
     8221 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8281 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8341 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8401 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     8461 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     8521 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8581 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     8641 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     8701 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     8761 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     8821 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     8881 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     8941 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     9001 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     9061 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     9121 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     9181 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     9241 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     9301 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     9361 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     9421 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     9481 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     9541 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     9601 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     9661 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     9721 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     9781 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     9841 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     9901 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     9961 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
    10021 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
    10081 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
    10141 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
    10201 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
    10261 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
    10321 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
    10381 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
    10441 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
    10501 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    10561 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    10621 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    10681 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    10741 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    10801 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    10861 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    10921 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    10981 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    11041 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    11101 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    11161 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    11221 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    11281 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
    11341 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
    11401 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
    11461 ggcagtgagc gcaacgcaat
//
