LOCUS       sequence-269136-       11830 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of  CHR2 mCherry from EF1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11830)
  TITLE     Emory Custom Cloning Core Plasmids - Oskar Laur
REFERENCE   2  (bases 1 to 11830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EF1a CHR2 mCherry WPRE
FEATURES             Location/Qualifiers
     source          1..11830
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1196..1215
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1196..1214
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1271..1280
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1277..2203
                     /codon_start=1
                     /product="H134R mutant of an N-terminal fragment of 
                     channelrhodopsin-2 from the alga Chlamydomonas reinhardtii 
                     (Nagel et al., 2005)"
                     /label=ChR2(H134R)
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVILIRLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     regulatory      2213..2222
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2219..2929
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2364..2382)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2632..2651
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2940..3528
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2993..3013)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3411..3422)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    join(3584..4159,4160..4162,4163..4171)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3752..3769)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3979..3998
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4172..4771
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4172..4191)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4668..4688
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(4885..4918)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             4943..5123
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5149..5166)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5155..5379
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5425..5853
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5512..5531)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5722..5743
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5862..5882)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5867..6196
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6047..6182
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6109..6128
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6244..6291
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6310..6684
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6814..6935
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6851..6870)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6905..6924
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6984..7000)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6984..7000)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6997..7019)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7008..7024
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7032..7038,7039..7056,7057..7062))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7077..7098
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7215..7232)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7386..7974)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7466..7485)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8145..8936,8937..9005))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8768..8787
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9006..9110)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9185..9204)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9376..9755
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9757..9955
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9910..9930
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9973..10153
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10200..10325
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10818..11051
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11236..11280
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11578..11695
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata
     1201 cgactcacta tagggagacc caagctggct aggtaagctt gatatcgaat tcctgcagcc
     1261 cgggggatcc gccaccatgg actatggcgg cgctttgtct gccgtcggac gcgaactttt
     1321 gttcgttact aatcctgtgg tggtgaacgg gtccgtcctg gtccctgagg atcaatgtta
     1381 ctgtgccgga tggattgaat ctcgcggcac gaacggcgct cagaccgcgt caaatgtcct
     1441 gcagtggctt gcagcaggat tcagcatttt gctgctgatg ttctatgcct accaaacctg
     1501 gaaatctaca tgcggctggg aggagatcta tgtgtgcgcc attgaaatgg ttaaggtgat
     1561 tctcgagttc ttttttgagt ttaagaatcc ctctatgctc taccttgcca caggacaccg
     1621 ggtgcagtgg ctgcgctatg cagagtggct gctcacttgt cctgtcatcc ttatccgcct
     1681 gagcaacctc accggcctga gcaacgacta cagcaggaga accatgggac tccttgtctc
     1741 agacatcggg actatcgtgt ggggggctac cagcgccatg gcaaccggct atgttaaagt
     1801 catcttcttt tgtcttggat tgtgctatgg cgcgaacaca ttttttcacg ccgccaaagc
     1861 atatatcgag ggttatcata ctgtgccaaa gggtcggtgc cgccaggtcg tgaccggcat
     1921 ggcatggctg tttttcgtga gctggggtat gttcccaatt ctcttcattt tggggcccga
     1981 aggttttggc gtcctgagcg tctatggctc caccgtaggt cacacgatta ttgatctgat
     2041 gagtaaaaat tgttgggggt tgttgggaca ctacctgcgc gtcctgatcc acgagcacat
     2101 attgattcac ggagatatcc gcaaaaccac caaactgaac atcggcggaa cggagatcga
     2161 ggtcgagact ctcgtcgaag acgaagccga ggccggagcc gtgccaggat ccgccaccat
     2221 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     2281 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     2341 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     2401 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     2461 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     2521 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     2581 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     2641 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     2701 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     2761 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     2821 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     2881 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtagg cttatcgata
     2941 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     3001 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     3061 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     3121 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     3181 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     3241 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     3301 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     3361 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     3421 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     3481 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtg
     3541 tacaagcggc cgcgtcgacg ggcccgcgga attccgcccc ccccccctct cyctccyccc
     3601 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     3661 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     3721 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     3781 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     3841 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     3901 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     3961 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     4021 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     4081 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     4141 tgaaaaacac gatgataata tggccacaac catgaccgag tacaagccca cggtgcgcct
     4201 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     4261 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     4321 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     4381 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     4441 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     4501 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     4561 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     4621 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     4681 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     4741 ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac
     4801 cgaaaggagc gcacgacccc atgcatcgaa caaaagaaaa ggggactgga agggctaatt
     4861 cactcccaac gaagacaaga tatcataact tcgtatagca tacattatac gaagttatcg
     4921 gctagctggt ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct
     4981 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     5041 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     5101 gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc
     5161 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     5221 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     5281 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     5341 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     5401 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     5461 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     5521 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     5581 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     5641 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     5701 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     5761 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     5821 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     5881 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5941 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6001 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     6061 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     6121 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     6181 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     6241 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     6301 aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc
     6361 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac
     6421 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg
     6481 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag
     6541 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc
     6601 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac
     6661 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc
     6721 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     6781 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     6841 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6901 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     6961 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     7021 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     7081 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7141 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7201 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7261 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7321 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7381 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7441 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7501 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7561 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     7621 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     7681 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     7741 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     7801 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     7861 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     7921 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     7981 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     8041 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     8101 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     8161 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     8221 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     8281 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     8341 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     8401 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     8461 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     8521 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     8581 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     8641 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     8701 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     8761 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     8821 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     8881 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     8941 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     9001 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     9061 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     9121 cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc
     9181 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
     9241 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
     9301 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
     9361 ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
     9421 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     9481 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     9541 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     9601 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     9661 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     9721 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
     9781 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     9841 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
     9901 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagcgcgt
     9961 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
    10021 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
    10081 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
    10141 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
    10201 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
    10261 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
    10321 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
    10381 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
    10441 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
    10501 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
    10561 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
    10621 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
    10681 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
    10741 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
    10801 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
    10861 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
    10921 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
    10981 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
    11041 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
    11101 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
    11161 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
    11221 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
    11281 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
    11341 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
    11401 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
    11461 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
    11521 aacggatcgg cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt
    11581 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
    11641 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
    11701 ttattacagg gacagcagag atccagtttg gttaattaat cgataagctt tgcaaagatg
    11761 gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg
    11821 agtgggaatt
//
