LOCUS       sequence-269151-       10383 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses soma targeted GtACR2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10383)
  AUTHORS   Fan LZ, Kheifets S, Bohm UL, Wu H, Piatkevich KD, Xie ME, Parot V, 
            Ha Y, Evans KE, Boyden ES, Takesian AE, Cohen AE
  TITLE     All-Optical Electrophysiology Reveals the Role of Lateral Inhibition
            in Sensory Processing in Cortical Layer 1.
  JOURNAL   Cell. 2020 Jan 23. pii: S0092-8674(20)30047-7. doi: 
            10.1016/j.cell.2020.01.001.
  PUBMED    31978320
REFERENCE   2  (bases 1 to 10383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Jan 23. pii: S0092-8674(20)30047-7. doi: 
            10.1016/j.cell.2020.01.001."
COMMENT     Sequence Label: UB061 (FCK-stGtACR2-fusionRed)
FEATURES             Location/Qualifiers
     source          1..10383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(72..863,864..932))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     695..714
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(933..1037)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1112..1131)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1303..1682
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1684..1882
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             1900..2080
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2127..2252
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2745..2978
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3163..3207
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3505..3622
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3681..3984
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3985..4188
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4185..4209
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4271..4280
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5219..5914
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
     misc_feature    6140..6728
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6193..6213)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6611..6622)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6731..6747)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6732..6748)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7253..7433
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7459..7476)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7465..7689
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7735..8163
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7822..7841)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8032..8053
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8172..8192)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8177..8506
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8357..8492
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8419..8438
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8554..8601
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8620..8994
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9124..9245
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9161..9180)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9215..9234
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9294..9310)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9294..9310)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9307..9329)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9318..9334
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9342..9348,9349..9366,9367..9372))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9387..9408
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9525..9542)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9696..10284)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9776..9795)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
       61 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
      121 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
      181 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
      241 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
      301 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
      361 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
      421 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
      481 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
      541 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
      601 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
      661 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
      721 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
      781 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
      841 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
      901 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
      961 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     1021 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
     1081 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     1141 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
     1201 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
     1261 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta
     1321 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     1381 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     1441 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     1501 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     1561 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     1621 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     1681 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     1741 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     1801 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     1861 ggtgggaggt ctatataagc agcgcgtttt gcctgtactg ggtctctctg gttagaccag
     1921 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     1981 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     2041 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     2101 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     2161 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     2221 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     2281 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     2341 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
     2401 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     2461 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
     2521 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
     2581 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     2641 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     2701 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     2761 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     2821 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     2881 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     2941 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     3001 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     3061 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     3121 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     3181 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     3241 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     3301 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     3361 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     3421 gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc aattctgcag
     3481 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     3541 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     3601 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggtt
     3661 aattaagctt gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     3721 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     3781 gggactttcc attgacgtca atgggtggag catttacggt aaactgccca cttggcagta
     3841 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     3901 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     3961 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     4021 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     4081 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     4141 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     4201 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc
     4261 tagaggatcc gccaccatgg catcacaggt cgtctatggg gaatgggcat caactcacac
     4321 cgagtgctac aatatgtcca gaatcgactc caccttcgtc tcactgctgc agctggtgtg
     4381 ggccgtggtc tcaggctgcc agacaatctt catgattagc agggctccca aggtcccttg
     4441 ggagtccgtg tacctgccct ttgtcgaatc tatcacttat gcactggcca gtactggcaa
     4501 cggaaccctg cagatgaggg acggcagatt ctttccttgg agccgaatgg cctcctggct
     4561 gtgcacctgt ccaatcatgc tgggacagat ttctaacatg gctctggtga agtacaaaag
     4621 tatcccactg aatcccattg cacaggccgc ttccatcatt agagtggtca tgggaatcac
     4681 cgctacaatt tctcccgcag agtatatgaa gtggctgttc tttttctttg gggccacctg
     4741 cctggtgttc gagtacagtg tggtcttcac aatctttcaa gtggggctgt atggctttga
     4801 atctgtcggg acacctctgg cacagaaggt ggtggtgagg atcaagatgc tgcgcctgat
     4861 cttctttatt gcttggacta tgttcccaat cgtgtggctg atttcaccca ccggagtctg
     4921 cgtgatccac gagaacgtga gcgccattct gtacctgctg gctgacggcc tgtgcaaaaa
     4981 tacatatgga gtgatcctgt ggagcactgc ctggggagtg ctggaaggga agtgggaccc
     5041 cgcatgtctg ccagggcagg agaaacccga agccgacgat ccttttggcc tgaatcacga
     5101 gaagaatgcc cctcctaacg acgaagtcaa tatcagaatg tttggaaggg ccgccaagag
     5161 caggatcacc agcgagggcg agtacatccc cctggaccag atcgacatca acgtggtgat
     5221 ggtgagcgag ctgattaagg agaacatgcc catgaagctg tacatggagg gcaccgtgaa
     5281 caaccaccac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac
     5341 catgagaatc aaggtcgtcg agggcggccc tctccccttc gccttcgaca tcctggctac
     5401 cagcttcatg tacggcagca gaaccttcat caagcaccct ccgggcatcc ccgacttctt
     5461 taagcagtcc ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg
     5521 cgtgctgacc gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa
     5581 ggttagaggg gtgaacttcc cagccaacgg ccctgtgatg cagaagaaaa cactcggctg
     5641 ggaggcctcc accgagacga tgtaccctgc tgacggagga ctggaaggcg catgtgacat
     5701 ggctctgaag ctcgtgggag gaggacacct gatctgcaac cttgagacaa catacagatc
     5761 caagaaaccc gctacgaacc tcaagatgcc cggcgtctac aacgtggacc acagactgga
     5821 aagaatcaag gaggccgacg atgagacata cgtcgagcag cacgaggtgg ctgtggccag
     5881 atactctact ggtggcgctg gtgatggagg taaacagtcc cagcctattc tgaacaccaa
     5941 agagatggcc ccccagagta aaccccctga ggagctggaa atgtcctcaa tgccctcccc
     6001 tgtggcacct cttcctgcaa gaacagaggg cgtgatcgac atgaggtcca tgagttctat
     6061 tgactctttt atctcatgcg ccaccgattt tccagaggct acgcggttta agtaagaatt
     6121 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     6181 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     6241 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     6301 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     6361 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     6421 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     6481 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     6541 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     6601 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     6661 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     6721 ctccccgcat cgataccgtc gacctcgaga cctagaaaaa catggagcaa tcacaagtag
     6781 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     6841 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     6901 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     6961 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa
     7021 ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt
     7081 accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca
     7141 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga
     7201 cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct
     7261 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7321 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7381 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg
     7441 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     7501 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     7561 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     7621 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     7681 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     7741 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     7801 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     7861 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     7921 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     7981 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     8041 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     8101 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     8161 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     8221 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     8281 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     8341 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     8401 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     8461 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     8521 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat
     8581 atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc
     8641 gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc
     8701 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc
     8761 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg
     8821 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg
     8881 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc
     8941 ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg
     9001 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     9061 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     9121 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     9181 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     9241 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     9301 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     9361 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     9421 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     9481 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     9541 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     9601 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     9661 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     9721 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     9781 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9841 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     9901 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     9961 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    10021 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    10081 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
    10141 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    10201 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    10261 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    10321 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    10381 acc
//
