LOCUS       sequence-269179-        1418 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express FL hnRNPF with N-terminal maltose binding protein tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Ryan VH, Perdikari TM, Naik MT, Saueressig CF, Lins J, Dignon GL, 
            Mittal J, Hart AC, Fawzi NL
  TITLE     Tyrosine phosphorylation regulates hnRNPA2 granule protein 
            partitioning and reduces neurodegeneration.
  JOURNAL   EMBO J. 2021 Feb 1;40(3):e105001. doi: 10.15252/embj.2020105001. 
            Epub 2020 Dec 22.
  PUBMED    33349959
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2021-02-1"; volume: "40(3):e105001. doi"; pages: " 
            10.15252/embj.2020105001. Epub 2020 Dec 22"
COMMENT     Sequence Label: pTHMT_hnRNPF_FL
FEATURES             Location/Qualifiers
     source          1..1418
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             51..71
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1329..1346
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1399..1417)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 gaaggagata taccatgggc agcgacaaaa ttcaccatca tcaccatcac gagaatctgt
       61 acttccaagg tcatatgatg ctgggtcctg agggtggcga gggtttcgtt gtcaagttgc
      121 gcggtctgcc gtggtcttgc tctgttgaag atgtccagaa cttcctgtcg gattgtacta
      181 tccacgatgg cgctgccggt gtgcacttca tctatacccg tgagggtcgc cagagcggtg
      241 aggccttcgt tgagctgggt tccgaagatg acgtcaaaat ggcgctgaaa aaagaccgtg
      301 aatctatggg ccaccgttat attgaagtgt tcaaaagcca ccgtaccgag atggactggg
      361 ttctgaagca cagcggtccg aacagcgcag atagcgcgaa tgatggtttt gttcgtctgc
      421 gtggcctgcc ttttggttgc accaaagaag aaattgtcca atttttctcg ggtctggaaa
      481 tcgttccgaa tggcattacg ctgccggttg atccggaagg caagattacc ggtgaggcat
      541 tcgtgcaatt tgctagccaa gaactggcag agaaagcact gggcaagcac aaggaacgta
      601 ttggtcaccg ctacatcgaa gtattcaaga gcagccaaga ggaagtgcgc agctatagcg
      661 atccgccgct gaagtttatg tccgtgcagc gtccgggtcc atacgaccgc ccgggcaccg
      721 cccgtcgtta tatcggtatc gtgaaacagg ccggcttgga gcgcatgcgc ccgggtgcgt
      781 atagcacggg ctacggtggc tacgaagagt actctggcct gagcgacggc tatggtttta
      841 cgacggacct gttcggtcgt gacttgagct attgcctgag cggtatgtac gaccaccgtt
      901 atggtgattc cgagtttacc gtccagtcta ccaccggtca ttgtgttcac atgcgtggtc
      961 tgccatacaa ggcaaccgag aatgacatct acaacttctt cagcccgctg aatccggtgc
     1021 gtgtccacat cgaaattggt ccggatggtc gtgtcacggg tgaggctgat gtcgaatttg
     1081 cgactcatga agaagccgtg gcggcgatga gcaaagaccg tgcgaacatg cagcatcgct
     1141 acattgagtt gtttctgaac agcaccacgg gcgcaagcaa cggtgcgtac tccagccaag
     1201 ttatgcaagg catgggtgtg agcgcggcac aggcgacgta cagcggcctg gagagccagt
     1261 ccgttagcgg ctgctatggt gcgggctata gcggtcagaa cagcatgggc ggctatgact
     1321 gactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag
     1381 ctgagttggc tgctgccacc gctgagcaat aactagca
//
