LOCUS       sequence-26918-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Chen B, Cepko CL
  TITLE     HDAC4 regulates neuronal survival in normal and diseased retinas.
  JOURNAL   Science. 2009 Jan 9. 323(5911):256-9.
  PUBMED    19131628
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2009 Jan 9. 323(5911):256-9."
COMMENT     Sequence Label: pbs/pU6-HDAC4 RNAi
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..51
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        48..361
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     280..300
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     complement(442..458)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(495..515)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(495..513)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(534..550)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(534..550)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(547..569)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    558..574
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(582..588,589..606,607..612))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    627..648
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(765..782)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atacgactcn ctatagggcg aattgggtac ccgctctaga actagtggat ccgacgccgc
       61 catctctagg cccgcgccgg ccccctcgca cagacttgtg ggagaagctc ggctactccc
      121 ctgccccggt taatttgcat ataatatttc ctagtaacta tagaggctta atgtgcgata
      181 aaagacagat aatctgttct ttttaatact agctacattt tacatgatag gcttggattt
      241 ctataagaga tacaaatact aaattattat tttaaaaaac agcacaaaag gaaactcacc
      301 ctaactgtaa agtaattgtg tgttttgaga ctataaatat cccttggaga aaagccttgt
      361 ttgggagatg ctggccatga agcagtgtgc tgtcctgctt catggccagc atctcccttt
      421 ttgaattcct gcagcccggg ggatccacta gttctagagc ggccgccacc gcggtggagc
      481 tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
      541 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
      601 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
      661 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
      721 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
      781 ctgcgctcgg tcgttcggct gcggcgagc
//
