LOCUS       sequence-269182-        1121 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  express HRP-1 with a C-terminal maltose binding protein tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Ryan VH, Perdikari TM, Naik MT, Saueressig CF, Lins J, Dignon GL, 
            Mittal J, Hart AC, Fawzi NL
  TITLE     Tyrosine phosphorylation regulates hnRNPA2 granule protein 
            partitioning and reduces neurodegeneration.
  JOURNAL   EMBO J. 2021 Feb 1;40(3):e105001. doi: 10.15252/embj.2020105001. 
            Epub 2020 Dec 22.
  PUBMED    33349959
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2021-02-1"; volume: "40(3):e105001. doi"; pages: " 
            10.15252/embj.2020105001. Epub 2020 Dec 22"
COMMENT     Sequence Label: pJ4M_HRP-1_FL
FEATURES             Location/Qualifiers
     source          1..1121
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             51..71
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gaaggagata taccatgggc agcgacaaaa tccaccatca tcaccaccac gaaaatctgt
       61 acttccaggg tcatatgacc gacgtcgaga ttaaagccga gaacggctcg ggtgatgcct
      121 ctctggagcc ggagaatctg cgcaagatct tcgttggcgg cctgacgagc aataccaccg
      181 atgacctgat gcgtgagttc tacagccaat ttggcgagat caccgacatt attgttatgc
      241 gcgatccgac tacgaagcgc agccgtggct ttggctttgt gacgtttagc ggcaagactg
      301 aagttgacgc tgcgatgaag caacgcccgc atatcattga cggcaagacc gtggacccga
      361 agcgtgcagt tccgcgtgat gacaaaaacc gcagcgaaag caacgttagc accaaacgcc
      421 tgtacgtgag cggtgtgcgt gaagatcaca ccgaagatat gctgaccgag tacttcacca
      481 aatatggtac cgtcacgaaa agcgagatta tcttggataa agcgacgcaa aagccgcgtg
      541 gtttcggctt tgtcaccttc gacgatcacg attccgttga ccagtgtgtg ctgcagaaaa
      601 gccacatggt gaatggccat cgctgcgacg tccgtaaggg tttgagcaaa gacgaaatgt
      661 ccaaggcaca aatgaaccgt gacagagaaa cgcgcggtgg tcgtagccgt gatggtcagc
      721 gtggcggcta caacggtggt ggcggtggtg gtggaggatg gggcggtcca gcacagcgtg
      781 gtggccctgg tgcgtacggc ggtcctggtg gtgggggtca gggtggctat ggcggcgatt
      841 atggtggcgg ctggggtcag caaggtggtg gtggccaggg tggttggggt ggtccgcagc
      901 agcaacaggg tggtggtggg tggggtcaac aaggcggtgg cggtcaaggt ggatggggtg
      961 gcccacagca acaacagcaa ggcggttggg gaggtccgca acaaggtggc ggcggtggag
     1021 gttggggcgg tcagggtcag cagcagggtg gctggggtgg tcaatctggt gcgcaacagt
     1081 gggctcatgc gcagggcggc aatcgtaact attaactcga g
//
