LOCUS       sequence-269243-        4711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for MpTubulin beta 3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Kimura S, Kodama Y
  TITLE     Actin-dependence of the chloroplast cold positioning response in the
            liverwort Marchantia polymorpha L.
  JOURNAL   PeerJ. 2016 Sep 28;4:e2513. eCollection 2016.
  PUBMED    27703856
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2016-09-28"; volume: "4"; pages: "e2513. eCollection 2016"
COMMENT     Sequence Label: pDONR207-MpTubulin beta 3
FEATURES             Location/Qualifiers
     source          1..4711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    42..141
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1483..1582)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1694..1713)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1772..1791)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(2365..2898)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3087..3092,3093..3109,3110..3115))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3772..4360
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4261..4280
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4495..4522)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4614..4700)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttccctactc tcgcgttaac gctagcatgg atctcgggcc ccaaataatg attttatttt
       61 gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa
      121 ctttgtacaa aaaagcaggc ttcatgagag aaattctcca catccagggt gggcaatgcg
      181 ggaaccagat cggagctaag ttttgggaag tgatctgtga tgagcatggg atcgacccca
      241 ctggaactta ccgcggagac tccgatcttc agttggagag gattaatgtt tactacaacg
      301 aggcaagtgg tggtaggtac gttccacgtg ctgtgctcat ggatctcgaa cccggaacga
      361 tggacagtgt tcgatctggt ccgtttggtc agatcttccg accagacaac tttgtctttg
      421 gccaaactgg ggccggtaac aattgggcca aggggcacta cacggaggga gcggaattga
      481 ttgactctgt tcttgacgtc gtgcgaaagg aggcggagag ctgtgattgc ctgcagggtt
      541 tccaagtctg ccactcgctg ggaggaggta ccggttcagg tatgggaacc ctgctgatat
      601 ccaagatccg tgaagaatac ccggacagga tgatgctcac tttctctgtc ttcccatcgc
      661 cgaaggtgtc cgatacagtc gtggagccct acaacgctac tctttctgtc caccagcttg
      721 tagaaaacgc ggacgagtgt atggttctgg ataatgaggc attgtacgac atttgcttcc
      781 gtactctgaa gctgaccacc cctacatttg gagatctcaa tcatctgata tcagccacaa
      841 tgagtggtgt tacttgctgc ctgaggtttc ccggtcaatt gaactctgac ctccgaaagc
      901 ttgccgtaaa cctgatccca ttccctcgac ttcacttctt catggtggga ttcgcacccc
      961 tcacgtctcg aggttcccaa caatatcgtg ctctgacagt gcctgaactc actcagcaaa
     1021 tgtgggatgc gaagaacatg atgtgcgccg ccgatcctcg tcatggacgt taccttacgg
     1081 cctcagccat gttccgcggg cgcatgagca ccaaggaagt cgacgaacag atgatcaatg
     1141 tccagaataa gaactcgtcg tacttcgtcg aatggatccc caacaacgtg aaatccagtg
     1201 tttgcgacat tcctcccaat gggcttaaaa tggcatccac tttcatcgga aactctactt
     1261 ctatccagga aatgtttaga cgagtgagcg agcagtttac tgccatgttc agaaggaaag
     1321 ctttcttgca ttggtacaca ggtgaaggaa tggacgagat ggagttcact gaggcagaga
     1381 gcaatatgaa tgatctggta tctgaatacc agcagtatca ggatgcaagc gccgaagatg
     1441 aaggagaata tgacgacggt gaagagcttg aagccaacta agacccagct ttcttgtaca
     1501 aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca
     1561 gtcaaaataa aatcattatt tgccatccag ctgcagctct ggcccgtgtc tcaaaatctc
     1621 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     1681 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
     1741 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     1801 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
     1861 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     1921 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     1981 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
     2041 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     2101 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     2161 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     2221 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     2281 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aggccgggaa
     2341 gccgatctcg gcttgaacga attgttaggt ggcggtactt gggtcgatat caaagtgcat
     2401 cacttcttcc cgtatgccca actttgtata gagagccact gcgggatcgt caccgtaatc
     2461 tgcttgcacg tagatcacat aagcaccaag cgcgttggcc tcatgcttga ggagattgat
     2521 gagcgcggtg gcaatgccct gcctccggtg ctcgccggag actgcgagat catagatata
     2581 gatctcacta cgcggctgct caaacttggg cagaacgtaa gccgcgagag cgccaacaac
     2641 cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta ctacggagca agttcccgag
     2701 gtaatcggag tccggctgat gttgggagta ggtggctacg tctccgaact cacgaccgaa
     2761 aagatcaaga gcagcccgca tggatttgac ttggtcaggg ccgagcctac atgtgcgaat
     2821 gatgcccata cttgagccac ctaactttgt tttagggcga ctgccctgct gcgtaacatc
     2881 gttgctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac
     2941 gcgcttgctg cttggatgcc cgaggcatag actgtacaaa aaaacagtca taacaagcca
     3001 tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc
     3061 gtgagcgcat acgctacttg cattacagtt tacgaaccga acaggcttat gtcaactggg
     3121 ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag
     3181 tcgaggcatt tctgtcctgg ctggcgaacg agcgcaaggt ttcggtctcc acgcatcgtc
     3241 aggcattggc ggccttgctg ttcttctacg gcaaggtgct gtgcacggat ctgccctggc
     3301 ttcaggagat cggaagacct cggccgtcgc ggcgcttgcc ggtggtgctg accccggatg
     3361 aagtggttcg catcctcggt tttctggaag gcgagcatcg tttgttcgcc caggactcta
     3421 gctatagttc tagtggttgg ctactaatag gttgtattga tgttggacga gtcggaatcg
     3481 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat
     3541 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt
     3601 ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg taacactggc
     3661 agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga
     3721 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3781 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3841 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3901 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3961 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4021 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4081 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4141 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4201 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4261 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4321 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4381 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     4441 tgattctgtg gataaccgta ttaccgctag ccaggaagag tttgtagaaa cgcaaaaagg
     4501 ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct
     4561 gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc
     4621 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac
     4681 tgagcctttc gttttatttg atgcctggca g
//
