LOCUS       sequence-269255-        3922 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Chen F, Kook H, Milewski R, Gitler AD, Lu MM, Li J, Nazarian R, 
            Schnepp R, Jen K, Biben C, Runke G, Mackay JP, Novotny J, Schwartz 
            RJ, Harvey RP, Mullins MC, Epstein JA
  TITLE     Hop is an unusual homeobox gene that modulates cardiac development.
  JOURNAL   Cell. 2002 Sep 20. 110(6):713-23.
  PUBMED    12297045
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2002 
            Sep 20. 110(6):713-23."
COMMENT     Sequence Label: pBS-mHopX
FEATURES             Location/Qualifiers
     source          1..3922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1081..1097)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1081..1097)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(1134..1152)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1173..1189)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1173..1189)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1186..1208)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1197..1213
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1221..1227,1228..1245,1246..1251))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1266..1287
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1404..1421)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1575..2163)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1655..1674)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2334..3125,3126..3194))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2957..2976
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3195..3299)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3326..3781
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3413..3432)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3623..3644
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     join(3908..3922,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(3922,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg ggtaccgggc
       61 cccccctcgt gccgtttttt ttttttcaac ttaacgtttt ttttaattta gaatataatt
      121 tttaacacaa aaagcagttc attttattca gcaaaataaa tttaacaagt tcatttaaat
      181 aaggtaaatt ctagactctg taactttttt tttccttagc tatttgcttt tctgcccctt
      241 ggaatccata gctgctatac taggttgctt tccaaggtaa cacagctgga aggaaaggaa
      301 ctgttcattc attcatatat atatatatat atatataatt tgaatgactc aaatacaccc
      361 acgttctcat tcaaccacca gtgtgtttag tgaagcagaa ggagggggca cagggaagtg
      421 aactcaggag gtgttcccct gcctgttctg ttatcttttc aatagaagcc aggcagctct
      481 gaaggggatg gtccatatga attactactg gaaggtgatg gccaagggca aacgaagacg
      541 gttcccacat catctttcat taaaatccaa aggcaagcca cacccctgcc cccagccaag
      601 ccatcacttt acacagaact gcatttagct ccctgttatt ttggattttt ttttttttta
      661 gttatgtaca taaaaaaaaa aacacattgt gttaaatagc aggacagcaa aacaatgaga
      721 cattcaacac tgcactgaaa catcaaaaca gcctgggtaa gccgagggaa ggaagaagag
      781 atggagttcc aagagcaagc tcaagggcct ggctccctag tccgtaacag atctgcattc
      841 cgaaggcaag ccttctgacc gccgccactc tgccaggcgc tgcttaaacc atttctgcgt
      901 ctgctcctcc gtgagacccg cctcggctgc gatgaggcac agcgtggtgg ggtccgggtg
      961 cttgttgacc ttgttgaagt tgtactccag gatctccacc tggtcctccg tggggccgct
     1021 cacggtctgc gccgacatgg tccgtgcgcg tgtgctcgtg ccgaatcctg cagcccgggg
     1081 gatccactag ttctagagcg gccgccaccg cggtggagct ccagcttttg ttccctttag
     1141 tgagggttaa tttcgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     1201 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     1261 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     1321 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     1381 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     1441 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     1501 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     1561 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     1621 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     1681 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     1741 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     1801 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     1861 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     1921 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     1981 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     2041 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     2101 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     2161 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     2221 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     2281 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     2341 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     2401 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     2461 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     2521 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     2581 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     2641 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     2701 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     2761 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     2821 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     2881 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     2941 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     3001 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     3061 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     3121 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     3181 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     3241 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     3301 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt
     3361 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     3421 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     3481 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     3541 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     3601 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     3661 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     3721 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     3781 tgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     3841 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     3901 gggttttccc agtcacgacg tt
//
