LOCUS       sequence-269257-        3523 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for AtOEP7(1–50).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Tanaka H, Sato M, Ogasawara Y, Hamashima N, Buchner O, Holzinger A, 
            Toyooka K, Kodama Y
  TITLE     Chloroplast aggregation during the cold-positioning response in the 
            liverwort Marchantia polymorpha.
  JOURNAL   J Plant Res. 2017 Jun 20. doi: 10.1007/s10265-017-0958-9.
  PUBMED    28634853
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Plant 
            Res. 2017 Jun 20. doi: 10.1007/s10265-017-0958-9."
COMMENT     Sequence Label: pDONR207-OEP7(1–50)
FEATURES             Location/Qualifiers
     source          1..3523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    31..130
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(284..383)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(495..514)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(573..592)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(1166..1699)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1888..1893,1894..1910,1911..1916))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      2573..3161
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3062..3081
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3296..3323)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3415..3501)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgcgttaacg ctagcatgga tctcgggccc caaataatga ttttattttg actgatagtg
       61 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaac tttgtacaaa
      121 aaagcaggct ccatgggaaa aacttcggga gcgaaacagg cgactgtggt ggtcgcagcg
      181 atggcgttag gatggttagc catagagatc gctttcaagc ctttcctcga taaattccgc
      241 tcctcaatcg acaaatctga cccaaccaaa gaccccgatg acgacccagc tttcttgtac
      301 aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc
      361 agtcaaaata aaatcattat ttgccatcca gctgcagctc tggcccgtgt ctcaaaatct
      421 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
      481 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg
      541 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
      601 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct
      661 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
      721 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
      781 atggttactc accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc
      841 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
      901 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
      961 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     1021 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt
     1081 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taggccggga
     1141 agccgatctc ggcttgaacg aattgttagg tggcggtact tgggtcgata tcaaagtgca
     1201 tcacttcttc ccgtatgccc aactttgtat agagagccac tgcgggatcg tcaccgtaat
     1261 ctgcttgcac gtagatcaca taagcaccaa gcgcgttggc ctcatgcttg aggagattga
     1321 tgagcgcggt ggcaatgccc tgcctccggt gctcgccgga gactgcgaga tcatagatat
     1381 agatctcact acgcggctgc tcaaacttgg gcagaacgta agccgcgaga gcgccaacaa
     1441 ccgcttcttg gtcgaaggca gcaagcgcga tgaatgtctt actacggagc aagttcccga
     1501 ggtaatcgga gtccggctga tgttgggagt aggtggctac gtctccgaac tcacgaccga
     1561 aaagatcaag agcagcccgc atggatttga cttggtcagg gccgagccta catgtgcgaa
     1621 tgatgcccat acttgagcca cctaactttg ttttagggcg actgccctgc tgcgtaacat
     1681 cgttgctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc cacggcgtaa
     1741 cgcgcttgct gcttggatgc ccgaggcata gactgtacaa aaaaacagtc ataacaagcc
     1801 atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg
     1861 cgtgagcgca tacgctactt gcattacagt ttacgaaccg aacaggctta tgtcaactgg
     1921 gttcgtgcct tcatccgttt ccacggtgtg cgtcacccgg caaccttggg cagcagcgaa
     1981 gtcgaggcat ttctgtcctg gctggcgaac gagcgcaagg tttcggtctc cacgcatcgt
     2041 caggcattgg cggccttgct gttcttctac ggcaaggtgc tgtgcacgga tctgccctgg
     2101 cttcaggaga tcggaagacc tcggccgtcg cggcgcttgc cggtggtgct gaccccggat
     2161 gaagtggttc gcatcctcgg ttttctggaa ggcgagcatc gtttgttcgc ccaggactct
     2221 agctatagtt ctagtggttg gctactaata ggttgtattg atgttggacg agtcggaatc
     2281 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
     2341 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
     2401 tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg
     2461 cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg
     2521 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     2581 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     2641 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     2701 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     2761 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     2821 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     2881 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     2941 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3001 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3061 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3121 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3181 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     3241 ctgattctgt ggataaccgt attaccgcta gccaggaaga gtttgtagaa acgcaaaaag
     3301 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
     3361 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
     3421 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
     3481 ctgagccttt cgttttattt gatgcctggc agttccctac tct
//
