LOCUS       sequence-269259-        4009 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for mouse Talin (mTalin).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Kimura S, Kodama Y
  TITLE     Actin-dependence of the chloroplast cold positioning response in the
            liverwort Marchantia polymorpha L.
  JOURNAL   PeerJ. 2016 Sep 28;4:e2513. eCollection 2016.
  PUBMED    27703856
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2016-09-28"; volume: "4"; pages: "e2513. eCollection 2016"
COMMENT     Sequence Label: pDONR207-mTalin
FEATURES             Location/Qualifiers
     source          1..4009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    45..144
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(784..883)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(995..1014)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1073..1092)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(1666..2199)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2388..2393,2394..2410,2411..2416))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3073..3661
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3562..3581
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3796..3823)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3915..4001)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cagttcccta ctctcgcgtt aacgctagca tggatctcgg gccccaaata atgattttat
       61 tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt tttataatgc
      121 caactttgta caaaaaagca ggcttcagcg gagcaggagc aggagcagga gcaggagcag
      181 gtactagtat cctagaagct gccaagtcca tcgctgcagc caccagtgca ctggtaaagg
      241 ctgcgtcagc agcccagagg gagctggtgg ctcaaggaaa ggtgggcgcc attccagcca
      301 atgcactgga tgatgggcag tggtcgcagg gcctcatttc cgctgcccgt atggtggctg
      361 cggccaccaa caatctgtgt gaagcagcta acgcagctgt ccagggccat gctagccagg
      421 agaaactcat ttcctcagcc aagcaagtag ctgcctccac agctcagctc ctggtagctt
      481 gcaaggtcaa agcagatcag gactctgagg caatgaaacg gctccaggct gctggcaatg
      541 cagtgaagag ggcctcagat aacctggtaa aggcggccca gaaagctgca gccttcgaag
      601 accaggagaa tgagacggtg gtggtgaagg agaaaatggt tgggggcatt gcccagatta
      661 tcgcagcaca ggaagagatg cttcggaagg aacgagagct ggaagaggct cggaaaaagc
      721 tcgcccagat ccggcagcag cagtacaagt tcttgccttc agagcttcga gacgagcact
      781 aagacccagc tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt
      841 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgcagctc
      901 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
      961 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     1021 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     1081 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
     1141 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     1201 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     1261 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc
     1321 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     1381 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     1441 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     1501 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     1561 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     1621 gaggggaaat taggccggga agccgatctc ggcttgaacg aattgttagg tggcggtact
     1681 tgggtcgata tcaaagtgca tcacttcttc ccgtatgccc aactttgtat agagagccac
     1741 tgcgggatcg tcaccgtaat ctgcttgcac gtagatcaca taagcaccaa gcgcgttggc
     1801 ctcatgcttg aggagattga tgagcgcggt ggcaatgccc tgcctccggt gctcgccgga
     1861 gactgcgaga tcatagatat agatctcact acgcggctgc tcaaacttgg gcagaacgta
     1921 agccgcgaga gcgccaacaa ccgcttcttg gtcgaaggca gcaagcgcga tgaatgtctt
     1981 actacggagc aagttcccga ggtaatcgga gtccggctga tgttgggagt aggtggctac
     2041 gtctccgaac tcacgaccga aaagatcaag agcagcccgc atggatttga cttggtcagg
     2101 gccgagccta catgtgcgaa tgatgcccat acttgagcca cctaactttg ttttagggcg
     2161 actgccctgc tgcgtaacat cgttgctgct gcgtaacatc gttgctgctc cataacatca
     2221 aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtacaa
     2281 aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg
     2341 tcaaggttct ggaccagttg cgtgagcgca tacgctactt gcattacagt ttacgaaccg
     2401 aacaggctta tgtcaactgg gttcgtgcct tcatccgttt ccacggtgtg cgtcacccgg
     2461 caaccttggg cagcagcgaa gtcgaggcat ttctgtcctg gctggcgaac gagcgcaagg
     2521 tttcggtctc cacgcatcgt caggcattgg cggccttgct gttcttctac ggcaaggtgc
     2581 tgtgcacgga tctgccctgg cttcaggaga tcggaagacc tcggccgtcg cggcgcttgc
     2641 cggtggtgct gaccccggat gaagtggttc gcatcctcgg ttttctggaa ggcgagcatc
     2701 gtttgttcgc ccaggactct agctatagtt ctagtggttg gctactaata ggttgtattg
     2761 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
     2821 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
     2881 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg
     2941 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat
     3001 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     3061 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3121 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3181 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     3241 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3301 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3361 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3421 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     3481 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3541 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3601 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3661 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     3721 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcta gccaggaaga
     3781 gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg
     3841 gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa
     3901 tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa
     3961 cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctgg
//
