LOCUS       sequence-26926-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Chen B, Cepko CL
  TITLE     HDAC4 regulates neuronal survival in normal and diseased retinas.
  JOURNAL   Science. 2009 Jan 9. 323(5911):256-9.
  PUBMED    19131628
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2009 Jan 9. 323(5911):256-9."
COMMENT     Sequence Label: PBS/pU6-HIF1 alpha RNAi plasmid 2
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     25..41
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     270..290
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     complement(431..447)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(484..504)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(484..502)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(523..539)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(523..539)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(536..558)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    547..563
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(571..577,578..595,596..601))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    616..637
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(754..771)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctatagggcg aattgggtac ccgctctaga actagtggat ccgacgccgc catctctagg
       61 cccgcgccgg ccccctcgca cagacttgtg ggagaagctc ggctactccc ctgccccggt
      121 taatttgcat ataatatttc ctagtaacta tagaggctta atgtgcgata aaagacagat
      181 aatctgttct ttttaatact agctacattt tacatgatag gcttggattt ctataagaga
      241 tacaaatact aaattattat tttaaaaaac agcacaaaag gaaactcacc ctaactgtaa
      301 agtaattgtg tgttttgaga ctataaatat cccttggaga anancntgtt tggggttgaa
      361 actcaagcaa ctggtgtgct gtcccagttg cttgagtttc aacccctttt tgaattcctg
      421 cagcccgggg gatccactag ttctagagcg gccgccaccg cggtggagct ccagcttttg
      481 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
      541 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
      601 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
      661 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
      721 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
      781 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
      841 atcaggggat aacgcaggaa agaacntgtg agcaaaaggn cagcaaag
//
