LOCUS       sequence-269280-       10948 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for expression of Citrine-fused AtOEP7(1-50) 
            (OEP7(1–50)-Citrine) to visualize chloroplast outer envelope 
            membrane in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10948)
  AUTHORS   Tanaka H, Sato M, Ogasawara Y, Hamashima N, Buchner O, Holzinger A, 
            Toyooka K, Kodama Y
  TITLE     Chloroplast aggregation during the cold-positioning response in the 
            liverwort Marchantia polymorpha.
  JOURNAL   J Plant Res. 2017 Jun 20. doi: 10.1007/s10265-017-0958-9.
  PUBMED    28634853
REFERENCE   2  (bases 1 to 10948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Plant 
            Res. 2017 Jun 20. doi: 10.1007/s10265-017-0958-9."
COMMENT     Sequence Label: pMpGWB106-OEP7(1–50)
FEATURES             Location/Qualifiers
     source          1..10948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     213..235
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     227..244
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     228..244
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        759..1104
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1126..1150
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1304..1328)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1339..1341,1342..1344,1345..2055)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1384..1405)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1645..1664)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1992..2013
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      2080..2332
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2365..2381)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2365..2381)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2378..2400)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2389..2405
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2413..2419,2420..2437,2438..2443))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2458..2479
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    2564..2738
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(2779..3804)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(3877..4222)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    4417..4441
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4962..5753
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5999..6587
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6488..6507
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6741..6758
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6773..6913
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6839..6858)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6999..7021
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7257..7451
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7517..8590)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9019..9648)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc tgctctagca ttcgccattc aggctgcgca actgttggga
      121 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
      181 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
      241 cagtgccaag cttgcatgcc tgcaggtccc cagattagcc ttttcaattt cagaaagaat
      301 gctaacccac agatggttag agaggcttac gcagcaggtc tcatcaagac gatctacccg
      361 agcaataatc tccaggaaat caaatacctt cccaagaagg ttaaagatgc agtcaaaaga
      421 ttcaggacta actgcatcaa gaacacagag aaagatatat ttctcaagat cagaagtact
      481 attccagtat ggacgattca aggcttgctt cacaaaccaa ggcaagtaat agagattgga
      541 gtctctaaaa aggtagttcc cactgaatca aaggccatgg agtcaaagat tcaaatagag
      601 gacctaacag aactcgccgt aaagactggc gaacagttca tacagagtct cttacgactc
      661 aatgacaaga agaaaatctt cgtcaacatg gtggagcacg acacacttgt ctactccaaa
      721 aatatcaaag atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta
      781 atatccggaa acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata
      841 gtggaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt
      901 gaagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
      961 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga tatctccact
     1021 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     1081 agttcatttc atttggagag aacacggggg actctagagt tatcaacaag tttgtacaaa
     1141 aaagcaggct ccatgggaaa aacttcggga gcgaaacagg cgactgtggt ggtcgcagcg
     1201 atggcgttag gatggttagc catagagatc gctttcaagc ctttcctcga taaattccgc
     1261 tcctcaatcg acaaatctga cccaaccaaa gaccccgatg acgacccagc tttcttgtac
     1321 aaagtggttg ataacagcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     1381 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     1441 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     1501 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgatgtg cttcgcccgc
     1561 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     1621 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     1681 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     1741 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     1801 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     1861 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     1921 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag
     1981 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     2041 gacgagctgt acaagtaagc ttagagctcg aatttccccg atcgttcaaa catttggcaa
     2101 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     2161 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     2221 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     2281 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg
     2341 gttccggaac caattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct
     2401 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     2461 agtgagctaa ctcacattag gctgaattag gcgcgcctat ttctgaattc aacacattgc
     2521 ggacgttttt aatgtactga attaacgccg aattaattcg ggggatctgg attttagtac
     2581 tggattttgg ttttaggaat tagaaatttt attgatagaa gtattttaca aatacaaata
     2641 catactaagg gtttcttata tgctcaacac atgagcgaaa ccctatagga accctaattc
     2701 ccttatctgg gaactactca cacattatta tggagaaact cgagcttgtc gatcgacaga
     2761 tcccggtcgg catctactct atttctttgc cctcggacga gtgctggggc gtcggtttcc
     2821 actatcggcg agtacttcta cacagccatc ggtccagacg gccgcgcttc tgcgggcgat
     2881 ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc
     2941 ccaagctgca tcatcgaaat tgccgtcaac caagctctga tagagttggt caagaccaat
     3001 gcggagcata tacgcccgga gtcgtggcga tcctgcaagc tccggatgcc tccgctcgaa
     3061 gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc cagaagaaga tgttggcgac
     3121 ctcgtattgg gaatccccga acatcgcctc gctccagtca atgaccgctg ttatgcggcc
     3181 attgtccgtc aggacattgt tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg
     3241 gcagtcctcg gcccaaagca tcagctcatc gagagcctgc gcgacggacg cactgacggt
     3301 gtcgtccatc acagtttgcc agtgatacac atggggatca gcaatcgcgc atatgaaatc
     3361 acgccatgta gtgtattgac cgattccttg cggtccgaat gggccgaacc cgctcgtctg
     3421 gctaagatcg gccgcagcga tcgcatccat agcctccgcg accggttgta gaacagcggg
     3481 cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt gaacggcggg agatgcaata
     3541 ggtcaggctc tcgctaaact ccccaatgtc aagcacttcc ggaatcggga gcgcggccga
     3601 tgcaaagtgc cgataaacat aacgatcttt gtagaaacca tcggcgcagc tatttacccg
     3661 caggacatat ccacgccctc ctacatcgaa gctgaaagca cgagattctt cgccctccga
     3721 gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc agaaacttct cgacagacgt
     3781 cgcggtgagt tcaggctttt tcatatctca ttgccccccg gatctgcgaa agctcgagag
     3841 agatagattt gtagagagag actggtgatt tcagcgtgtc ctctccaaat gaaatgaact
     3901 tccttatata gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc
     3961 agtggagata tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc
     4021 cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg
     4081 aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac
     4141 tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat
     4201 taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt
     4261 cttggagtag acgagagtgt cgtgctccac catgttggca agctgctcta gccaatacgc
     4321 aaaccgaatt cagaaataaa ttcagcctaa ttcggcgtta attcagtaca ttaaaaacgt
     4381 ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc
     4441 accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg
     4501 cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct
     4561 catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg
     4621 atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcaa
     4681 ttcgggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc
     4741 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     4801 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
     4861 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
     4921 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgggggaa gcggtgatcg
     4981 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga
     5041 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg
     5101 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga
     5161 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag
     5221 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac
     5281 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga
     5341 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag
     5401 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa
     5461 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag
     5521 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg
     5581 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag
     5641 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg
     5701 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taatgtctag
     5761 ctagaaattc gttcaagccg acgccgcttc gcggcgcggc ttaactcaag cgttagatgc
     5821 actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt attattttta
     5881 agcgtgcata ataagcccta cacaaattgg gagatatatc atgcatgacc aaaatccctt
     5941 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6001 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6061 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6121 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     6181 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6241 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6301 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6361 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6421 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6481 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6541 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6601 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     6661 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     6721 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
     6781 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
     6841 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     6901 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     6961 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     7021 gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt
     7081 cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt aggccagcca
     7141 tttttgagcg gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt agggagcgca
     7201 gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt gcgctggcca gacagttatg
     7261 cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag gcggaaaaat
     7321 cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca atgtacggct
     7381 ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc ccaatgtacg
     7441 tgctatccac aggaaagaga ccttttcgac ctttttcccc tgctagggca atttgcccta
     7501 gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc
     7561 gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac tccggcaggt
     7621 catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct ccggcgctgc
     7681 cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct gcggcgcggc
     7741 gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa
     7801 ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa tcagctagct
     7861 cgatctcgat gtactccggc cgcccggttt cgctctttac gatcttgtag cggctaatca
     7921 aggcttcacc ctcggatacc gtcaccaggc ggccgttctt ggccttcttc gtacgctgca
     7981 tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac caggtcgtct ttctgctttc
     8041 cgccatcggc tcgccggcag aacttgagta cgtccgcaac gtgtggacgg aacacgcggc
     8101 cgggcttgtc tcccttccct tcccggtatc ggttcatgga ttcggttaga tgggaaaccg
     8161 ccatcagtac caggtcgtaa tcccacacac tggccatgcc ggccggccct gcggaaacct
     8221 ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg
     8281 acagacggaa aacggccacg tccatgatgc tgcgactatc gcgggtgccc acgtcataga
     8341 gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta atcgacggcg
     8401 caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc gcttgccacg
     8461 attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc gcggccttca
     8521 acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg gatggtttgc
     8581 gaccgctcac gccgattcct cgggcttggg ggttccagtg ccattgcagg gccggcagac
     8641 aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc attccacggc
     8701 gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta aaattcatct
     8761 actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca gatagcagct
     8821 cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga tcctccgccg
     8881 gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc aacgttgcag
     8941 ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt ttgctcattt
     9001 tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca gcgcctggac
     9061 ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg cggcggcagt
     9121 gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct cgtacccggc
     9181 cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa
     9241 ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc
     9301 ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga agtcggctgc
     9361 cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc
     9421 gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt
     9481 tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg gatcggaatc
     9541 gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg ttgcgatggt
     9601 cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc gttccccttg
     9661 cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg caagctgttt
     9721 tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc ggccaagctg
     9781 gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg cagccgcacg
     9841 gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc cgcctcgatc
     9901 tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct
     9961 cttggcgttc attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga
    10021 aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt
    10081 acagggtcga gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg cggccttcct
    10141 ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg cgggggccaa
    10201 acttcacgcc tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg
    10261 aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac catgcggccg gccggcgtgg
    10321 tggtgtcggc ccacggctct gccaggctac gcaggcccgc gccggcctcc tggatgcgct
    10381 cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg gtcactgtca
    10441 caacgtcgcc agggcgtagg tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg
    10501 tgccggtgat cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt
    10561 ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata gcccagcagg ccagcggcgg
    10621 cgctcttgtt catggcgtaa tgtctccggt tctagtcgca agtattctac tttatgcgac
    10681 taaaacacgc gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt
    10741 aggacttgtg cgacatgtcg ttttcagaag acggctgcac tgaacgtcag aagccgactg
    10801 cactatagca gcggaggggt tggatcaaag tactttgatc ccgaggggaa ccctgtggtt
    10861 ggcatgcaca tacaaatgga cgaacggata aaccttttca cgccctttta aatatccgat
    10921 tattctaata aacgctcttt tctcttag
//
