LOCUS       sequence-26929-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Hwang HW, Wentzel EA, Mendell JT
  TITLE     A hexanucleotide element directs microRNA nuclear import.
  JOURNAL   Science. 2007 Jan 5. 315(5808):97-100.
  PUBMED    17204650
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2007 Jan 5. 315(5808):97-100."
COMMENT     Sequence Label: pcDNA3-miR29b
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     63..82
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        63..81
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(561..578)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    567..792
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cnnggggcan tagcgagctc tctggctact agagaaccca ctgcttactg gcttatcgaa
       61 attaatacga ctcactatag ggagacccaa gctggctagc gcatgctctc ccatcaataa
      121 caaattcagt gacatcagtt tatgaatata tgaaatttgc caaagctctg tttagaccac
      181 tgagtaactc acagctaggt ttcaactttt cctttctagg ttgtcttggg tttattgtaa
      241 gagagcatta tgaagaaaaa aatagatcat aaagcttctt caggaagctg gtttcatatg
      301 gtggtttaga tttaaatagt gattgtctag caccatttga aatcagtgtt cttgggggag
      361 accagctgcg ctgcactacc aacagcaaaa gaagtgaatg ggacagctct gaagtatttg
      421 aaagcaacag caggatggct gtgagaacct gcctcacatg tagctgaccc cttcctcacc
      481 cctgccaaca gtggtggcat atatcacaaa tggcagtcag gtcctcgagt ctagagggcc
      541 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
      601 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
      661 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtgtgng
      721 gtggggcang acagcaaggg ggaggatggg aagacaatag caggcatgct ggggatgcgg
      781 tgggctctat ggctctgagg cgaaagacca gctgggctct aggggtnccc accnnctgtn
      841 cgccatagcc gcgggggngg taccncacgg ccctcctgca ccc
//
