LOCUS       sequence-26931-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Hwang HW, Wentzel EA, Mendell JT
  TITLE     Cell-cell contact globally activates microRNA biogenesis.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Apr 28;106(17):7016-21. doi: 
            10.1073/pnas.0811523106.
  PUBMED    19359480
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1073/pnas"; 
            journalName: "Proc Natl Acad Sci U S A"; date: "2009-04-28- 28"; 
            volume: "106"; issue: "17"; pages: "7016-21"
COMMENT     Sequence Label: pcDNA3-miR17
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..83
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        64..82
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(515..532)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    521..728
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ccgggggcan tagcagagct ctctggctac tagagaaccc actgcttact ggcttatcga
       61 aattaatacg actcactata gggagaccca agctggctag cgtttaaact taagcttggt
      121 accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcca gcacagtggc
      181 ggccgctcga gtcttcccca ttagggatta tgctgaattt gtatggttta tagttgttag
      241 agtttgaggt gttaattcta attatctatt tcaaatttag caggaaaaaa gagaacatca
      301 ccttgtaaaa ctgaagattg tgaccagtca gaataatgtc aaagtgctta cagtgcaggt
      361 agtgatatgt gcatctactg cagtgaaggc acttgtagca ttatggtgac agctgcctcg
      421 ggaagccaag ttgggcttta aagtgcaggg cctgctgatg ttgagtgctt tttgttcctc
      481 gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
      541 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
      601 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
      661 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
      721 gctggggatg cggtgggctc tatgnctctg aggcgaaaaa accagtgggg ctctaggggt
      781 atcccacgcg cctgtacgcc ataacgcgcg gggtgtgtta cccacggacg ctcctgcacg
      841 cctacgcgct cttccttctc ctncttccnc ngtncgntcc gcagntacgg g
//
