LOCUS       sequence-26932-e         677 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Hwang HW, Wentzel EA, Mendell JT
  TITLE     Cell-cell contact globally activates microRNA biogenesis.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Apr 28;106(17):7016-21. doi: 
            10.1073/pnas.0811523106.
  PUBMED    19359480
REFERENCE   2  (bases 1 to 677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1073/pnas"; 
            journalName: "Proc Natl Acad Sci U S A"; date: "2009-04-28- 28"; 
            volume: "106"; issue: "17"; pages: "7016-21"
COMMENT     Sequence Label: pcDNA3-miR21
FEATURES             Location/Qualifiers
     source          1..677
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(635..652)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcttttcct ttaggagcat tatgagcatt atgtcagaat agaatagaat
      121 tggggttcga tcttaacagg ccagaaatgc ctgggttttt ttggtttgtt tttgtttttg
      181 tttttttatc aaatcctgcc tgactgtctg cttgttttgc ctaccatcgt gacatctcca
      241 tggctgtacc accttgtcgg gtagcttatc agactgatgt tgactgttga atctcatggc
      301 aacaccagtc gatgggctgt ctgacatttt ggtatctttc atctgaccat ccatatccaa
      361 tgttctcatt taaacattac ccagcatcat tgtttataat cagaaactct ggtccttctg
      421 tctggtggca cttagagtct tttgtgccat aatgcagcag tatggaggga ggattttatg
      481 gagaaatggg gatagtcttc atgaccagct agcgtttaaa cttaagcttg gtaccgagct
      541 cggatccact agtccagtgt ggtggaattc tgcagatatc cagcacagtg gcggccgctc
      601 gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
      661 ccatctgttg tttgccc
//
