LOCUS       sequence-269348-         678 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TRE3GV promoter and DsRedExpress2 in TUPV5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 678)
  TITLE     Leonard Lab mMoClo Plasmids
REFERENCE   2  (bases 1 to 678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD1023 TRE3GV DsRedExpress2 in TU5
FEATURES             Location/Qualifiers
     source          1..678
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..678
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(181..201)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     593..616
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 atggatagca ctgagaacgt catcaagccc ttcatgcgct tcaaggtgca catggagggc
       61 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggcaagcc ctacgagggc
      121 acccagaccg ccaagctcca ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
      181 ctgtcccccc agttccagta cggctccaag gtgtacgtga agcaccccgc cgacatcccc
      241 gactacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      301 gacggcggcg tggtgaccgt gacccaggac tcctccctcc aggacggcac cttcatctac
      361 cacgtgaagt tcatcggcgt gaacttcccc tccgacggcc ccgtaatgca gaagaaaact
      421 ctgggctggg agccctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag
      481 atccacaagg cgctgaagct gaagggcggc ggccactacc tggtggagtt caagtcaatc
      541 tacatggcca agaagcccgt gaagctgccc ggctactact acgtggactc caagctggac
      601 atcacctccc acaacgagga ctacaccgtg gtggagcagt acgagcgcgc cgaggcccgc
      661 caccacctgt tccagtag
//
