LOCUS       sequence-26935-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Hwang HW, Wentzel EA, Mendell JT
  TITLE     Cell-cell contact globally activates microRNA biogenesis.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Apr 28;106(17):7016-21. doi: 
            10.1073/pnas.0811523106.
  PUBMED    19359480
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1073/pnas"; 
            journalName: "Proc Natl Acad Sci U S A"; date: "2009-04-28- 28"; 
            volume: "106"; issue: "17"; pages: "7016-21"
COMMENT     Sequence Label: pGL3con-miR15aBul
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        73..269
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      120..255
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      299..308
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(371..389)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ggagggcgac atttctctat cgataggtac cgagctctta cgcgtgctag cccgggctcg
       61 agatctgcga tctgcatctc aattagtcag caaccatagt cccgccccta actccgccca
      121 tcccgcccct aactccgccc agttccgccc attctccgcc ccatcgctga ctaatttttt
      181 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
      241 gcttttttgg aggcctaggc ttttgcaaaa agcttggcat tccggtactg ttggtaaagc
      301 caccatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc cgctggaaga
      361 tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac
      421 aattgctttt acagatgcac atatcgaggt ggacatcact tacgctgagt acttcgaaat
      481 gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt
      541 cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg
      601 agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatggg
      661 catttcgcag cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt
      721 gcanancaac cccnnnncat ttaanttttt nnttntggat ctaaaacgga tacagggatt
      781 cagtcgatgt accgtncgtc antctcatct actccggttn atgatacnat ttggcaatnc
      841 tnnnangaaa aaaatncntg ncagatctcg ganacgtngc tangg
//
