LOCUS       sequence-269354-         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TetON3G under hEF1alpha in TUPV4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  TITLE     Leonard Lab mMoClo Plasmids
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pJM469  EF1alpha TetON3G in TU4
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..747
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 atgtctagac tggacaagag caaagtcata aactctgctc tggaattact caatggagtc
       61 ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc
      121 ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat cgagatgctg
      181 gacaggcatc atacccactc ctgccccctg gaaggcgagt catggcaaga ctttctgcgg
      241 aacaacgcca agtcataccg ctgtgctctc ctctcacatc gcgacggggc taaagtgcat
      301 ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg
      361 tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt gggccacttt
      421 acactgggct gcgtattgga ggaacaggag catcaagtag caaaagagga aagagagaca
      481 cctaccaccg attctatgcc cccacttctg aaacaagcaa ttgagctgtt cgaccggcag
      541 ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct ggagaaacag
      601 ctaaagtgcg aaagcggcgg gccgaccgac gcccttgacg attttgactt agacatgctc
      661 ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat
      721 tttgaccttg acatgctccc cgggtaa
//
