LOCUS       sequence-269391-       11526 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for expression of Citrine-fused Lifeact 
            (Lifeact-Citrine) to visualize actin filaments in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11526)
  AUTHORS   Kimura S, Kodama Y
  TITLE     Actin-dependence of the chloroplast cold positioning response in the
            liverwort Marchantia polymorpha L.
  JOURNAL   PeerJ. 2016 Sep 28;4:e2513. eCollection 2016.
  PUBMED    27703856
REFERENCE   2  (bases 1 to 11526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2016-09-28"; volume: "4"; pages: "e2513. eCollection 2016"
COMMENT     Sequence Label: pMpGWB403-Lifeact-Citrine
FEATURES             Location/Qualifiers
     source          1..11526
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     213..235
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     227..244
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     228..244
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2004..2028
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2031..2081
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     CDS             join(2097..2099,2100..2102,2103..2813)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2142..2163)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2403..2422)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2750..2771
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(2818..2842)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      2874..3126
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3159..3175)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3159..3175)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3172..3194)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3183..3199
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3207..3213,3214..3231,3232..3237))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3252..3273
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    3358..3532
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(3589..4386)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3700..3719)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4310..4329
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4455..4800)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(4492..4512)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    4995..5019
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5540..6331
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6577..7165
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7066..7085
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7319..7336
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7351..7491
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7417..7436)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7577..7599
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7835..8029
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8095..9168)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9597..10226)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc tgctctagca ttcgccattc aggctgcgca actgttggga
      121 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
      181 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
      241 cagtgccaag cttggcccgg gcaaatgagt cacacacatt gttgagagac atatcaaaat
      301 aactcaattg taaggtaagt tgatagattt cccagttaaa tgcattattt taacaatatt
      361 tgtatctcaa attttcaaaa tcatatcaaa tttgcagatc ttgatgatga ttcacaactc
      421 tgtcttcgat ttttgattgt taacgggtat cgatatttgg gtaacttaaa aagaatttgc
      481 taaagatctt aaaaaacaga ttaaaaaaac ctttcaaaat agctaatcct ttaaatccgt
      541 ttcacaatat aatgctcatt gatgataatc gaaaagattc cattttaaag tgcatataaa
      601 ttgcttaatt atctctttct gtctcgtaat attttgttct ctcctattgg atcggacccg
      661 catcttctcg atcagttcac tatactaata tttggtcaac ttcaagagag attagaactt
      721 cgttctccaa agtgtaaagg ccgacaagca gaaaaggaaa cttcaatcag aagccttcta
      781 ggttttcccg cgtcccaaaa agcgaaggga ggacggggag gacggtgagg ccagaagaag
      841 aaagcaacag taaaatctca tgtgattgcg tgcatgtcat ggatctatgg gatgctagtc
      901 caccgtttgg aagtatgtgc agaaggagaa aggcgcggga ggccaaaagc gacttcggcc
      961 acctctctac tttctagaaa gtttgctcgc agcttgtaat aagcgcggaa aaggtactcg
     1021 aaaaaccgga gcaatcggtg tggtatcagc agtcgggttc gctaaaccta cgtcttctta
     1081 ttttggaatg ttaaattgaa agggcgtgcc ggccctaagc acggacgatg tgacagacgg
     1141 aggagtgtga gcaattcgga tgacgatgct ggccggcagt cacgacaaag agtccgaagg
     1201 ggcatggaca gggcaatata taaatggaaa agaggctttg aatattccta acccttcgtc
     1261 gtcggcctcc caacttgccc tcgcatgctg aattggccgc ctatctgcga atggccggcc
     1321 gccgacccta agtcgcaata acttgaacgg agccgatgca aagtccttat cgaacgctcg
     1381 ttattataaa cggggcagac ctaggtattt aattatatca aaaccctccg caatttcgct
     1441 aatcattccc taatttcttc tactttggcg tctagtgctg tggtcgcgct tgctctcaaa
     1501 gctcgctggg aggcagtgga gcgtctggct taaaatcgat cgccgcaacc ctagagttcc
     1561 tttcacttct ttctctcttg cgtggagagc atcgacatcg aagagctgca tcttgtcttc
     1621 tggtatgcgc cttgtgccct cgtctctgta cgtcgttacg attgctcgat attgtgattg
     1681 tctagacctg aaacccgact ggagctttta cgtggtgtcg attggggcct cgagcgagtg
     1741 attttttagg ttcgttttag ccaagtacat cccactttgg tcagtcctgt tatctcggta
     1801 aggtgttcag ttcggtctct ggtgcggagg tgcctcgtaa gtttcctgca tgatagattt
     1861 gtctatcctt gacttgaaga ggagtcgaag gtcagcgagg ttggctgtgt gctatggacg
     1921 tcggtgctcc ttggttccgt gcttctatgg gtgttgcctc aacagtattg atgtgcctgc
     1981 agaaaggttg actagagtta tcaacaagtt tgtacaaaaa agcaggcttc atgggcgtgg
     2041 ccgacctgat caagaagttc gagagcatca gcaaggagga gggtggctct ggaggtatgg
     2101 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     2161 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     2221 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     2281 tgaccacctt cggctacggc ctgatgtgct tcgcccgcta ccccgaccac atgaagcagc
     2341 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     2401 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     2461 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     2521 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     2581 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     2641 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     2701 tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     2761 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtgagacc
     2821 cagctttctt gtacaaagtg gttgataaca gcgcttagag ctcgaatttc cccgatcgtt
     2881 caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta
     2941 tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt
     3001 tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag
     3061 aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac
     3121 tagatcggga attggttccg gaaccaattc gtaatcatgt catagctgtt tcctgtgtga
     3181 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     3241 tggggtgcct aatgagtgag ctaactcaca ttaggctgaa ttaggcgcgc ctatttctga
     3301 attcaacaca ttgcggacgt ttttaatgta ctgaattaac gccgaattaa ttcgggggat
     3361 ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt
     3421 tacaaataca aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat
     3481 aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga aactcgagct
     3541 tgtcgatcga ctctagctag aggatcgatc cgaaccccag agtcccgctc agaagaactc
     3601 gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac
     3661 gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc
     3721 tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg
     3781 gccattttcc accatgatat tcggcaagca ggcatcgcca tgtgtcacga cgagatcctc
     3841 gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg
     3901 ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc
     3961 gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg
     4021 ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag
     4081 atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc
     4141 gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc
     4201 ctggagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg
     4261 cgctgacagc cgaaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata
     4321 gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat
     4381 ccccatggtc gatcgacaga tctgcgaaag ctcgagagag atagatttgt agagagagac
     4441 tggtgatttc agcgtgtcct ctccaaatga aatgaacttc cttatataga ggaagggtct
     4501 tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatatc acatcaatcc
     4561 acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg
     4621 ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg
     4681 caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag
     4741 atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca
     4801 atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg
     4861 tgctccacca tgttggcaag ctgctctagc caatacgcaa accgaattca gaaataaatt
     4921 cagcctaatt cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt
     4981 ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca acagctcccc
     5041 gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc
     5101 gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga
     5161 agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt
     5221 gattgtaacg atgacagagc gttgctgcct gtgatcaatt cgggcacgaa cccagtggac
     5281 ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc
     5341 cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta
     5401 tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     5461 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     5521 taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc
     5581 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     5641 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     5701 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     5761 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     5821 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     5881 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     5941 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     6001 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     6061 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     6121 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     6181 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     6241 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
     6301 aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac
     6361 gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata attgctcaca
     6421 gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat aagccctaca
     6481 caaattggga gatatatcat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6541 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6601 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     6661 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     6721 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     6781 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6841 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6901 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6961 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     7021 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     7081 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     7141 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     7201 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     7261 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     7321 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     7381 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
     7441 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
     7501 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     7561 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     7621 cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg
     7681 tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc
     7741 gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt
     7801 ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag
     7861 agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc
     7921 agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt
     7981 ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagagacc
     8041 ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga
     8101 accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc
     8161 ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg
     8221 cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt
     8281 gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg
     8341 gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt
     8401 gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg
     8461 cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt
     8521 caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa
     8581 ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa
     8641 cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc
     8701 ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc
     8761 ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc
     8821 gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc
     8881 catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg
     8941 ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg
     9001 ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc
     9061 ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc
     9121 cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg
     9181 ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct tacgcctggc
     9241 caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga
     9301 ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat
     9361 ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg
     9421 taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt
     9481 catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg
     9541 acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa
     9601 atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg
     9661 ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc
     9721 gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg
     9781 ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc
     9841 gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa
     9901 gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag
     9961 tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg
    10021 tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg
    10081 gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg
    10141 gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc
    10201 tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc
    10261 gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt
    10321 ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg
    10381 catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct
    10441 cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt
    10501 cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc
    10561 cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc
    10621 ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc
    10681 aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg
    10741 cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc
    10801 ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc
    10861 ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc
    10921 caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg
    10981 ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg
    11041 gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa
    11101 cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc
    11161 ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg
    11221 tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg
    11281 ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt
    11341 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg
    11401 gatcaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata caaatggacg
    11461 aacggataaa ccttttcacg cccttttaaa tatccgatta ttctaataaa cgctcttttc
    11521 tcttag
//
