LOCUS       sequence-269398-        9508 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Mtb MCM small and large subunit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9508)
  AUTHORS   Ruetz M, Campanello GC, Purchal M, Shen H, McDevitt L, Gouda H, 
            Wakabayashi S, Zhu J, Rubin EJ, Warncke K, Mootha VK, Koutmos M, 
            Banerjee R
  TITLE     Itaconyl-CoA forms a stable biradical in methylmalonyl-CoA mutase 
            and derails its activity and repair.
  JOURNAL   Science. 2019 Nov 1;366(6465):589-593. doi: 10.1126/science.aay0934.
  PUBMED    31672889
REFERENCE   2  (bases 1 to 9508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2019-11-1- 1"; volume: "366"; issue: 
            "6465"; pages: "589-593"
COMMENT     Sequence Label: pMtb MCM
FEATURES             Location/Qualifiers
     source          1..9508
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             83..100
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             113..133
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     promoter        2051..2069
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2070..2094
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             4454..4498
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(4536..4554)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4550..4597
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4634..5089
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4721..4740)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4931..4952
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(5204..5995,5996..6064))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5827..5846
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6065..6157)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6238..6826
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6727..6746
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6980..6997
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7012..7154
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7080..7099)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7240..7262
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7256..7447)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8019..9101)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9063..9082
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9102..9179)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9385..9404
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc ctgagaacct
      121 gtacttccag agcgtgtcca ttgatgtacc cgagcgtgcc gacctagaac aggttcgcgg
      181 gcgctggcgc aacgcggttg ccggtgtgct gtccaagagc aaccgtaccg actcagcaca
      241 actcggcgat caccccgagc ggctgctgga tacccagacc gctgacgggt tcgccatccg
      301 ggccctctac accgcgttcg acgagctccc ggagccgccg ttgccgggcc agtggccctt
      361 tgtgcgcggc ggagacccgc tgcgcgacgt gcattccggc tggaaggtcg ccgaggcgtt
      421 tcccgccaac ggtgcgacgg ccgacaccaa cgcggcggtg ctggccgcgc tcggcgaggg
      481 ggtcagcgcg ctgctgatcc gggtggggga gtcgggtgtg gcgcctgacc ggctcacggc
      541 gctgctgtcc ggggtgtatc tgaacctggc gccggtcatc ctcgacgccg gcgccgacta
      601 ccgcccggcc tgcgacgtca tgctggcgct ggtcgcccag ctcgatcccg gccagcgcga
      661 caccctgtcg atcgacctgg gcgccgaccc gctgacggcg tcgctgcgcg atcgtcccgc
      721 cccgccgatc gaggaggtcg tcgcggtcgc atcccgggcg gccggcgaac gtgggcttcg
      781 tgcgatcacc gtcgacggac cggccttcca caacctgggc gcgaccgcgg ccaccgaact
      841 cgcggccacc gtcgcggccg cggtggccta cctgcgggtg ctcaccgaat ccgggctcgt
      901 ggtgagtgac gcgctgcggc agatcagctt ccggctcgcc gccgacgacg accagttcat
      961 gacgctggcc aagatgcggg ctctacgtca actgtgggcg cgggtcgccg aggtcgtggg
     1021 cgacccgggt ggcggcgcgg ccgtcgtgca cgcggagacg tcgctaccga tgatgaccca
     1081 gcgtgatccg tgggtgaaca tgctgcgctg cacgctggcg gccttcggcg ccggtgtcgg
     1141 tggcgcggac accgtgctgg tgcacccgtt cgacgtggcg attcccggcg gctttcccgg
     1201 cacggcggcc ggctttgcgc gccggatcgc tcgcaacacc caactgctgc ttttagaaga
     1261 gtcgcatgtc ggcagggtgc tcgatcccgc cggcgggtcg tggttcgtcg aagagctcac
     1321 cgaccggctg gctcggcgcg cctggcagcg tttccaggcc atcgaggccc gtggcggctt
     1381 cgtcgaggcc cacgacttcc tggccggcca gatcgccgag tgcgccgccc gccgcgccga
     1441 cgacatcgcc catcggcgcc tggcgatcac cggcgtcaac gaatacccga acctgggcga
     1501 acccgcgctg ccgcccggtg atccgacatc gccggtgcgc cgctacgctg ccggattcga
     1561 agcattgcgc gatcgatccg atcaccacct agcccgcact ggcgcacggc cgcgggtgct
     1621 gttgctgccg ttgggtccgc tggccgagca caacatccgg acgaccttcg ccaccaacct
     1681 gctggcgtcc ggcggcatcg aggcgatcga cccgggaacg gttgatgcgg gcaccgtcgg
     1741 gaatgccgtt gccgatgccg gttcgcccag cgttgccgtg atctgcggca ccgatgcgcg
     1801 ctaccgggac gaggttgccg acattgtgca agcggcccga gccgccggtg tttcgagggt
     1861 gtacctcgcg ggtcccgaga aggcgttggg agatgccgca caccggcccg acgagttttt
     1921 gaccgcgaaa atcaatgtgg tgcaagcctt gtcgaatctg ctgacgcggt tgggggccta
     1981 agcttgcggc cgcataatgc ttaagtcgaa cagaaagtaa tcgtattgta cacggccgca
     2041 taatcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttccccatct
     2101 tagtatatta gttaagtata agaaggagat atacatatga tgacaaccaa gacacccgtg
     2161 atcggcagct tcgccggcgt tccgctgcat agcgagcgtg ccgcgcaatc gcccacagag
     2221 gccgcggtgc acacgcatgt cgccgccgcc gcggcggcgc acgggtacac gcccgaacag
     2281 ttggtgtggc acacgccgga aggcattgac gtcacaccgg tatacatcgc cgccgaccgg
     2341 gccgccgccg aagccgaggg ctacccgctg cacagcttcc cgggcgagcc cccctttgtg
     2401 cgcggcccct atccgacgat gtatgtgaac cagccgtgga ccatccgcca gtacgccggg
     2461 ttttccaccg ccgcggattc caatgcgttt taccgacgca acctggccgc cggccagaag
     2521 gggctgtcgg tggccttcga tctggccacc caccgcggct acgactccga ccatccccgc
     2581 gtgcagggcg atgtcggaat ggccggtgtg gcaatcgatt ccattctcga catgcgacag
     2641 ctgttcgacg gcatcgacct gtcgaccgtg agcgtgtcga tgacgatgaa cggtgcggtg
     2701 ctgccgatcc tggcgctgta tgtggttgcc gccgaggagc agggcgtggc gccggagcag
     2761 ctggccggca ccatccagaa cgacatcctc aaagagttca tggtccgcaa cacctacatc
     2821 tatccgccga agccgtcgat gcggatcatc tccgacatct tcgcctacac cagcgccaag
     2881 atgcccaagt tcaactccat ctccatttcc ggctatcaca tccaagaagc cggtgccacg
     2941 gcggatttgg agctggccta caccctggcc gacggcgtcg actacatcag ggcgggcctg
     3001 aacgccggcc tggacatcga cagcttcgcg ccccggctat cgttcttctg gggcatcggg
     3061 atgaatttct ttatggaggt cgccaaactg cgggccggcc ggttgctgtg gagtgagctg
     3121 gtcgcacagt tcgcacccaa gagcgccaaa tccctttcgc tgcgtacaca ttcgcaaaca
     3181 tcggggtggt cactgaccgc ccaggatgtg ttcaacaacg tggcgcgcac atgcatcgag
     3241 gcgatggccg ccacccaggg gcacacccag tcgctgcaca ccaacgccct ggacgaggcg
     3301 ctggcgctgc ccaccgattt ttcggcccgc atcgcgcgca acacccagct ggtgttgcag
     3361 caggagtcgg gcaccacgcg gccgatcgac ccgtgggggg gctcctacta tgtggagtgg
     3421 ctgacccatc ggctcgcgcg gcgagcccgg gcgcacatcg ccgaggtcgc tgaacatggc
     3481 ggcatggcgc aggccatcag cgacggcatc cccaagctgc gcatcgagga ggcggccgcg
     3541 cgcacccagg cccgcatcga ctccggtcag caaccggtgg tcggggtgaa taaataccag
     3601 gtgcccgagg accacgagat cgaggtgctc aaggtcgaaa acagccgggt gcgcgccgag
     3661 cagctggcca aactgcagcg gctgcgggca ggccgggacg agccggcggt acgggccgcg
     3721 ctggccgagc tgacccgcgc cgccgccgag caaggacgcg ccggagcaga cgggctgggc
     3781 aataatctgc tggccctggc catcgacgcc gcccgggccc aggccaccgt gggcgagatc
     3841 tccgaagcgc tggagaaggt gtacggacgg caccgggccg agatccgtac catttccggg
     3901 gtctaccgcg acgaagttgg aaaggccccc aacatcgcag ccgcaaccga gctagtggag
     3961 aagttcgccg aggccgacgg ccgccggccc aggattctga tcgccaagat gggccaggac
     4021 ggccacgacc gcgggcagaa ggtgatcgcg accgcgttcg ccgacatcgg gttcgacgtc
     4081 gacgtggggt cgctgttttc cacccccgag gaggtggcgc gtcaggccgc cgacaacgac
     4141 gtgcacgtga tcggggtgtc ctcgctggcc gccggccatc tgacgctggt gccggcgctg
     4201 cgcgacgcgt tggcgcaggt gggcaggccc gacatcatga tcgtggtcgg tggtgtcatc
     4261 ccgccgggcg acttcgacga gctgtacgcc gccggggcca ccgccatttt cccgccgggg
     4321 acggtgattg ccgacgcggc gattgacctg ctgcacaggc tggccgagcg gctggggtac
     4381 acgctggatt agggatctca attggatatc ggccggccac gcgatcgctg acgtcggtac
     4441 cctcgagtct ggtaaagaaa ccgctgctgc gaaatttgaa cgccagcaca tggactcgtc
     4501 tactagcgca gcttaattaa cctaggctgc tgccaccgct gagcaataac tagcataacc
     4561 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg
     4621 attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4681 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4741 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4801 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     4861 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4921 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     4981 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     5041 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc tggcggcacg
     5101 atggcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     5161 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     5221 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     5281 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     5341 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     5401 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     5461 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     5521 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     5581 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     5641 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     5701 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     5761 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     5821 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     5881 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     5941 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     6001 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     6061 tcatactctt cctttttcaa tcatgattga agcatttatc agggttattg tctcatgagc
     6121 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gtcatgacca aaatccctta
     6181 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6241 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6301 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6361 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     6421 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6481 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6541 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6601 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     6661 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6721 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6781 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6841 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     6901 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     6961 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     7021 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt
     7081 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     7141 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     7201 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     7261 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     7321 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     7381 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     7441 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     7501 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     7561 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     7621 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     7681 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     7741 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     7801 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     7861 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgctagtc atgccccgcg
     7921 cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga gatcccggtg
     7981 cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct ttccagtcgg
     8041 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     8101 gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc
     8161 ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg
     8221 cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc ttcggtatcg
     8281 tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt aatggcgcgc
     8341 attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca
     8401 ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc
     8461 gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc
     8521 gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc
     8581 agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact gttgatgggt
     8641 gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca
     8701 atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg ttgcgcgaga
     8761 agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc
     8821 acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg
     8881 tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt
     8941 tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc
     9001 gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca
     9061 ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga
     9121 ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc gatggtgtcc
     9181 gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt
     9241 gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag
     9301 tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa
     9361 gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc
     9421 tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tcgatctcga
     9481 tcccgcgaaa ttaatacgac tcactata
//
