LOCUS       sequence-269399-       11433 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for expression of Citrine-fused mouse Talin 
            (Citrine-mTalin) to visualize actin filaments in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11433)
  AUTHORS   Kimura S, Kodama Y
  TITLE     Actin-dependence of the chloroplast cold positioning response in the
            liverwort Marchantia polymorpha L.
  JOURNAL   PeerJ. 2016 Sep 28;4:e2513. eCollection 2016.
  PUBMED    27703856
REFERENCE   2  (bases 1 to 11433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2016-09-28"; volume: "4"; pages: "e2513. eCollection 2016"
COMMENT     Sequence Label: pMpGWB105-mTalin
FEATURES             Location/Qualifiers
     source          1..11433
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     213..235
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     227..244
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     228..244
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        759..1104
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(1125..1127,1128..1130,1131..1841)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1170..1191)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1431..1450)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1778..1799
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1851..1875
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2515..2539)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      2565..2817
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2850..2866)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2850..2866)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2863..2885)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2874..2890
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2898..2904,2905..2922,2923..2928))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2943..2964
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    3049..3223
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(3264..4289)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(4362..4707)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    4902..4926
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5447..6238
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6484..7072
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6973..6992
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7226..7243
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7258..7398
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7324..7343)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7484..7506
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7742..7936
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8002..9075)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9504..10133)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc tgctctagca ttcgccattc aggctgcgca actgttggga
      121 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
      181 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
      241 cagtgccaag cttgcatgcc tgcaggtccc cagattagcc ttttcaattt cagaaagaat
      301 gctaacccac agatggttag agaggcttac gcagcaggtc tcatcaagac gatctacccg
      361 agcaataatc tccaggaaat caaatacctt cccaagaagg ttaaagatgc agtcaaaaga
      421 ttcaggacta actgcatcaa gaacacagag aaagatatat ttctcaagat cagaagtact
      481 attccagtat ggacgattca aggcttgctt cacaaaccaa ggcaagtaat agagattgga
      541 gtctctaaaa aggtagttcc cactgaatca aaggccatgg agtcaaagat tcaaatagag
      601 gacctaacag aactcgccgt aaagactggc gaacagttca tacagagtct cttacgactc
      661 aatgacaaga agaaaatctt cgtcaacatg gtggagcacg acacacttgt ctactccaaa
      721 aatatcaaag atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta
      781 atatccggaa acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata
      841 gtggaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt
      901 gaagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
      961 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga tatctccact
     1021 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     1081 agttcatttc atttggagag aacacggggg actctagaat gagcatggtg agcaagggcg
     1141 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     1201 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     1261 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg
     1321 gctacggcct gatgtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
     1381 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     1441 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     1501 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     1561 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     1621 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     1681 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt
     1741 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     1801 ccgccgccgg gatcactctc ggcatggacg agctgtacaa ggctgttatc acaagtttgt
     1861 acaaaaaagc aggcttcagc ggagcaggag caggagcagg agcaggagca ggtactagta
     1921 tcctagaagc tgccaagtcc atcgctgcag ccaccagtgc actggtaaag gctgcgtcag
     1981 cagcccagag ggagctggtg gctcaaggaa aggtgggcgc cattccagcc aatgcactgg
     2041 atgatgggca gtggtcgcag ggcctcattt ccgctgcccg tatggtggct gcggccacca
     2101 acaatctgtg tgaagcagct aacgcagctg tccagggcca tgctagccag gagaaactca
     2161 tttcctcagc caagcaagta gctgcctcca cagctcagct cctggtagct tgcaaggtca
     2221 aagcagatca ggactctgag gcaatgaaac ggctccaggc tgctggcaat gcagtgaaga
     2281 gggcctcaga taacctggta aaggcggccc agaaagctgc agccttcgaa gaccaggaga
     2341 atgagacggt ggtggtgaag gagaaaatgg ttgggggcat tgcccagatt atcgcagcac
     2401 aggaagagat gcttcggaag gaacgagagc tggaagaggc tcggaaaaag ctcgcccaga
     2461 tccggcagca gcagtacaag ttcttgcctt cagagcttcg agacgagcac taagacccag
     2521 ctttcttgta caaagtggtg ataacctaga gctcgaattt ccccgatcgt tcaaacattt
     2581 ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat
     2641 ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga
     2701 gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa
     2761 tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg
     2821 aattggttcc ggaaccaatt cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat
     2881 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     2941 taatgagtga gctaactcac attaggctga attaggcgcg cctatttctg aattcaacac
     3001 attgcggacg tttttaatgt actgaattaa cgccgaatta attcggggga tctggatttt
     3061 agtactggat tttggtttta ggaattagaa attttattga tagaagtatt ttacaaatac
     3121 aaatacatac taagggtttc ttatatgctc aacacatgag cgaaacccta taggaaccct
     3181 aattccctta tctgggaact actcacacat tattatggag aaactcgagc ttgtcgatcg
     3241 acagatcccg gtcggcatct actctatttc tttgccctcg gacgagtgct ggggcgtcgg
     3301 tttccactat cggcgagtac ttctacacag ccatcggtcc agacggccgc gcttctgcgg
     3361 gcgatttgtg tacgcccgac agtcccggct ccggatcgga cgattgcgtc gcatcgaccc
     3421 tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc tctgatagag ttggtcaaga
     3481 ccaatgcgga gcatatacgc ccggagtcgt ggcgatcctg caagctccgg atgcctccgc
     3541 tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa gaagatgttg
     3601 gcgacctcgt attgggaatc cccgaacatc gcctcgctcc agtcaatgac cgctgttatg
     3661 cggccattgt ccgtcaggac attgttggag ccgaaatccg cgtgcacgag gtgccggact
     3721 tcggggcagt cctcggccca aagcatcagc tcatcgagag cctgcgcgac ggacgcactg
     3781 acggtgtcgt ccatcacagt ttgccagtga tacacatggg gatcagcaat cgcgcatatg
     3841 aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc
     3901 gtctggctaa gatcggccgc agcgatcgca tccatagcct ccgcgaccgg ttgtagaaca
     3961 gcgggcagtt cggtttcagg caggtcttgc aacgtgacac cctgtgaacg gcgggagatg
     4021 caataggtca ggctctcgct aaactcccca atgtcaagca cttccggaat cgggagcgcg
     4081 gccgatgcaa agtgccgata aacataacga tctttgtaga aaccatcggc gcagctattt
     4141 acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga ttcttcgccc
     4201 tccgagagct gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa cttctcgaca
     4261 gacgtcgcgg tgagttcagg ctttttcata tctcattgcc ccccggatct gcgaaagctc
     4321 gagagagata gatttgtaga gagagactgg tgatttcagc gtgtcctctc caaatgaaat
     4381 gaacttcctt atatagagga agggtcttgc gaaggatagt gggattgtgc gtcatccctt
     4441 acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct
     4501 ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca
     4561 tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc accttccttt
     4621 tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc
     4681 gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac tgtatctttg
     4741 atattcttgg agtagacgag agtgtcgtgc tccaccatgt tggcaagctg ctctagccaa
     4801 tacgcaaacc gaattcagaa ataaattcag cctaattcgg cgttaattca gtacattaaa
     4861 aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc
     4921 ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat
     4981 acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact
     5041 ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac
     5101 ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg
     5161 atcaattcgg gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa
     5221 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     5281 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg
     5341 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     5401 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatgg gggaagcggt
     5461 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     5521 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     5581 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     5641 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     5701 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     5761 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     5821 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     5881 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     5941 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     6001 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     6061 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     6121 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     6181 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg
     6241 tctagctaga aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta
     6301 gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat
     6361 ttttaagcgt gcataataag ccctacacaa attgggagat atatcatgca tgaccaaaat
     6421 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     6481 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6541 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6601 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     6661 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6721 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6781 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6841 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6901 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6961 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7021 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7081 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     7141 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     7201 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
     7261 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct
     7321 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
     7381 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     7441 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     7501 cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg
     7561 gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccgtaggcc
     7621 agccattttt gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga
     7681 gcgcagcgac cgaagggtag gcgctttttg cagctcttcg gctgtgcgct ggccagacag
     7741 ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga
     7801 aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta
     7861 cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat
     7921 gtacgtgcta tccacaggaa agagaccttt tcgacctttt tcccctgcta gggcaatttg
     7981 ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg
     8041 gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg
     8101 caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc
     8161 gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc
     8221 gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg
     8281 gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc
     8341 tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct
     8401 aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct tcttcgtacg
     8461 ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg
     8521 ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac
     8581 gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg ttagatggga
     8641 aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg gccctgcgga
     8701 aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc gtcggtcacg
     8761 cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc
     8821 atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct tcctaatcga
     8881 cggcgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag cggccgcttg
     8941 ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc
     9001 cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg ggccggatgg
     9061 tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg
     9121 cagacaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc
     9181 acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt
     9241 catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag
     9301 cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc
     9361 cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt
     9421 tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct
     9481 cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc
     9541 tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg
     9601 gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac
     9661 ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg
     9721 acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc
     9781 aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg
     9841 gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg
     9901 aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca
     9961 acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg
    10021 gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg
    10081 atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc
    10141 ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc
    10201 tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca
    10261 agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc
    10321 gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct
    10381 cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg
    10441 ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc
    10501 acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc
    10561 gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc
    10621 ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg
    10681 gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat
    10741 tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg
    10801 cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat
    10861 gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac
    10921 tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg
    10981 cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc
    11041 ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc
    11101 ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat
    11161 gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt
    11221 aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc
    11281 gactgcacta tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg
    11341 tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat
    11401 ccgattattc taataaacgc tcttttctct tag
//
