LOCUS       sequence-269404-        2853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AausFP1 - bright green fluorescent protein expression from A. 
            australis under EF1a-HTLV promoter in minimal backbone plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2853)
  TITLE     Constitutive promoter minimal backbone plasmids for optimized 
            expression in mammalian cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: EF1-alpha-AausFP1-Z1
FEATURES             Location/Qualifiers
     source          1..2853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..273
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             286..554
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      619..628
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1378..1499
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1389..1408)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1443..1462
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1658..2032)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(2042..2060)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2052..2098)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     rep_origin      complement(2171..2759)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2251..2270)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcggccgcct gcagaagctt tactaactag tggatctgcg atcgctccgg tgcccgtcag
       61 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
      121 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
      181 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
      241 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
      301 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
      361 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
      421 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
      481 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
      541 cgccgttaca gatccaagct gtgaccggcg cctacaattc tgcagtcgac ggtaccgcgg
      601 gcccgggatc caccggtcgc caccatgggt tcctatggtg ctctgctgtt tagggagaag
      661 ataccttacg tggtcgaaat ggaaggcgac gtcgaaggca tgaaattcag cgtgcggggc
      721 aaggggcacg gagatgccaa tacagggaag atcgaggctt cctttatatg tactaccggc
      781 gagctgcctg ttccctggag ctctatactc accaccgtga cctatggtgc ccaatgcttc
      841 gctaagtacc cgaacgacat aaaggattat cctaagtccg caatgcccga ggggtatgtc
      901 caggaacgta ccataacatt cgagaatgat ggagtttaca aaacgcgggc cgaggttact
      961 tatgaaaagg gctccgtcta caatagggtg acactgaatg ggtccgggtt caagaagggc
     1021 ggaaatattc tgggaaagaa gctggagttc aattataatc cccactgtat ttacgtcctg
     1081 cccgacgttc agaacaacgg cattaagtgt tatattaata tcgtgcacga tgtgatcggc
     1141 ggcggtcaga tcatcgcagc ccatcagcag ctgaacacac cactcggcgg cggtcccgta
     1201 gatatcccgc attatcacca catccaggcc cataccatcc tctcaaagga cccgaaggag
     1261 accagggacc acatgaatgt ggtcgaagta tttcgtgcca ttgactgtaa gactgcttac
     1321 gcctgatagg cggccgcgac tctagatgta cacatatggc tagctggcca gacatgataa
     1381 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     1441 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     1501 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     1561 aaagcaagta aaacctctac aaatgtggta tggaattctg gactcacaaa gaaaaaacgc
     1621 ccggtgtgca agaccgagcg ttctgaacaa tttatcctca gtcctgctcc tctgccacaa
     1681 agtgcacgca gttgccggcc gggtcgcgca gggcgaactc ccgcccccac ggctgctcgc
     1741 cgatctcggt catggccggc ccggaggcgt cccggaagtt cgtggacacg acctccgacc
     1801 actcggcgta cagctcgtcc aggccgcgca cccacaccca ggccagggtg ttgtccggca
     1861 ccacctggtc ctggaccgcg ctgatgaaca gggtcacgtc gtcccggacc acaccggcga
     1921 agtcgtcctc cacgaagtcc cgggagaacc cgagccggtc ggtccagaac tcgaccgctc
     1981 cggcgacgtc gcgcgcggtg agcaccggaa cggcactggt caacttggcc atgatggccc
     2041 tcctatagtg agtcgtatta tactatgccg atatactatg ccgatgatta attgtcaagg
     2101 atcctaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     2161 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     2221 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2281 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2341 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     2401 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     2461 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     2521 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2581 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     2641 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2701 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2761 aagatccttt gatcttttct acggggtctg acgctcagtg aaaactcacg ttaagggatt
     2821 ttggtcatgg ctagttaatt aacatttaaa tca
//
