LOCUS       sequence-269407-        2930 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AausFP1 - bright green fluorescent protein expression from A. 
            australis under CMV promoter in minimal backbone plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2930)
  TITLE     Constitutive promoter minimal backbone plasmids for optimized 
            expression in mammalian cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CMV-AausFP1-Z1
FEATURES             Location/Qualifiers
     source          1..2930
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        83..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    613..693
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      695..704
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    1454..1575
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1465..1484)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1519..1538
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1734..2108)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(2118..2136)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2128..2174)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     rep_origin      complement(2247..2835)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2327..2346)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cggccgcctg cagaagcttt actaactagt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga
      601 accgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
      661 gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atgggttcct atggtgctct
      721 gctgtttagg gagaagatac cttacgtggt cgaaatggaa ggcgacgtcg aaggcatgaa
      781 attcagcgtg cggggcaagg ggcacggaga tgccaataca gggaagatcg aggcttcctt
      841 tatatgtact accggcgagc tgcctgttcc ctggagctct atactcacca ccgtgaccta
      901 tggtgcccaa tgcttcgcta agtacccgaa cgacataaag gattatccta agtccgcaat
      961 gcccgagggg tatgtccagg aacgtaccat aacattcgag aatgatggag tttacaaaac
     1021 gcgggccgag gttacttatg aaaagggctc cgtctacaat agggtgacac tgaatgggtc
     1081 cgggttcaag aagggcggaa atattctggg aaagaagctg gagttcaatt ataatcccca
     1141 ctgtatttac gtcctgcccg acgttcagaa caacggcatt aagtgttata ttaatatcgt
     1201 gcacgatgtg atcggcggcg gtcagatcat cgcagcccat cagcagctga acacaccact
     1261 cggcggcggt cccgtagata tcccgcatta tcaccacatc caggcccata ccatcctctc
     1321 aaaggacccg aaggagacca gggaccacat gaatgtggtc gaagtatttc gtgccattga
     1381 ctgtaagact gcttacgcct gataggcggc cgcgactcta gatgtacaca tatggctagc
     1441 tggccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     1501 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     1561 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     1621 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatgga attctggact
     1681 cacaaagaaa aaacgcccgg tgtgcaagac cgagcgttct gaacaattta tcctcagtcc
     1741 tgctcctctg ccacaaagtg cacgcagttg ccggccgggt cgcgcagggc gaactcccgc
     1801 ccccacggct gctcgccgat ctcggtcatg gccggcccgg aggcgtcccg gaagttcgtg
     1861 gacacgacct ccgaccactc ggcgtacagc tcgtccaggc cgcgcaccca cacccaggcc
     1921 agggtgttgt ccggcaccac ctggtcctgg accgcgctga tgaacagggt cacgtcgtcc
     1981 cggaccacac cggcgaagtc gtcctccacg aagtcccggg agaacccgag ccggtcggtc
     2041 cagaactcga ccgctccggc gacgtcgcgc gcggtgagca ccggaacggc actggtcaac
     2101 ttggccatga tggccctcct atagtgagtc gtattatact atgccgatat actatgccga
     2161 tgattaattg tcaaggatcc taagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     2221 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     2281 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2341 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2401 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     2461 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     2521 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     2581 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2641 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     2701 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2761 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2821 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtgaaaa
     2881 ctcacgttaa gggattttgg tcatggctag ttaattaaca tttaaatcag
//
