LOCUS       sequence-269455-        8492 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Env gene from HIV patient plasma in pcDNA3.1 expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8492)
  AUTHORS   Joshi A, Sedano M, Beauchamp B, Punke EB, Mulla ZD, Meza A, Alozie 
            OK, Mukherjee D, Garg H
  TITLE     HIV-1 Env Glycoprotein Phenotype along with Immune Activation 
            Determines CD4 T Cell Loss in HIV Patients.
  JOURNAL   J Immunol. 2016 Feb 15;196(4):1768-79. doi: 
            10.4049/jimmunol.1501588. Epub 2016 Jan 13.
  PUBMED    26764036
REFERENCE   2  (bases 1 to 8492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.1501588"; journalName: "J Immunol"; date: 
            "2016-02-15- 15"; volume: "196"; issue: "4"; pages: "1768-79"
COMMENT     Sequence Label: pHP025Env
FEATURES             Location/Qualifiers
     source          1..8492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(50..70)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        55..384
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      235..370
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     297..316
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             451..1245
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(505..524)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1115..1134
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1421..1542
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1458..1477)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1512..1531
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1591..1607)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1591..1607)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1604..1626)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1615..1631
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1639..1645,1646..1663,1664..1669))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1684..1705
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1822..1839)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1993..2581)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2073..2092)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2752..3543,3544..3612))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3375..3394
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3613..3717)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3792..3811)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3983..4362
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4363..4566
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4517..4537
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4611..4630
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4611..4629
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5402..5413
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             7261..7287
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             7738..7779
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7789..7806
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7829..7846)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7835..8059
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(8105..8492,1..41)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8192..8211)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8402..8423
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
       61 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
      121 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
      181 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
      241 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
      301 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
      361 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
      421 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
      481 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
      541 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
      601 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
      661 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
      721 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
      781 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
      841 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
      901 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
      961 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     1021 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     1081 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     1141 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     1201 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     1261 tcgcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     1321 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     1381 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     1441 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     1501 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     1561 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     1621 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     1681 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     1741 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     1801 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1861 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1921 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1981 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2041 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2101 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2161 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2221 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2281 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2341 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2401 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     2461 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2521 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2581 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2641 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     2701 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     2761 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     2821 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     2881 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     2941 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     3001 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     3061 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     3121 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     3181 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     3241 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     3301 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     3361 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     3421 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     3481 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     3541 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     3601 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     3661 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     3721 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
     3781 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
     3841 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
     3901 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
     3961 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
     4021 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     4081 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     4141 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     4201 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     4261 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     4321 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     4381 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     4441 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     4501 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     4561 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
     4621 cactataggg agacccaagc tggctagtta agcttggtac cgagctcgga tccagtaccc
     4681 ttcaccgatc aagctttagg catctcctat ggcaggaaga agcggagaca gcgacgcaga
     4741 cctcctcaag actgtcaaat tgatcaagcg tctctatcaa agcagtaagt agtacatgta
     4801 atgcctcttt tagcaataac aatagtagca ttagtaatag cagcaatact agcaatagtt
     4861 gtgtggtcta tagtattcat agaatatagg aaaatattaa gacaaagaaa aatagacagg
     4921 ttaattgata gaataagaga aagagaagaa gacagtggca atgagagtga aggggatcag
     4981 gaggagttat ccagaattat ggagaggggg catcttgctc cttgggatgt taatgatctg
     5041 tagtgctaca gacaatttgt gggtcacagt ctattatggg gtacctgtgt ggagagaagc
     5101 aaacaccact ctattctgtg catcagatgc caaagcatat gataaagagg tgcataatgt
     5161 ttgggcctcg catgcctgtg tacctacaga ccctaaccca caagaaatgt tattggaaaa
     5221 tgtgacagaa tattttaaca tgtggaaaaa tgatatggta gaacagatgc aggaggatat
     5281 aatcagctta tgggatcaaa gcctaaaacc atgtgtaaaa ttaaccccac tctgtgtcac
     5341 tctacattgt agtaatatta ccaatatcaa taatagtact gctggtggca atagtagcaa
     5401 tatagagggg agagaagaga taaagaactg ctcttttaat attaccacaa acataagaaa
     5461 aaaggtgcaa ggagaatatg cattgtttta ttcaactgat gtagtacaaa tagaaaatga
     5521 taactctagc tatatgataa gaagttgtaa tacctcagtc attacacagg cctgtccaaa
     5581 aatatctttt gagccaattc ccatacatta ttgtgccccg gctggttttg cgattctaaa
     5641 atgtaatgag acgaagttca gtggaaaagg aaattgtagt aaggtcagca cagtacaatg
     5701 tacacatgga attagaccag tagtatcaac tcaattgctg ttaaatggca gtctagcaga
     5761 agaagaggtg gtaattagat ctgacaattt ctcagacaat gctagaaaca tactagtaca
     5821 gctgaatgag actgtagaaa ttaactgtac aaggcccaac aacaatacaa gaaaaagtat
     5881 acatatagga ccaggaagag cattttatac aacaggagaa ataataggag acataagaca
     5941 ggcacattgt aacattagtg aagcaaaatg gaatcagacc ttaaaaaggg tagctaaaaa
     6001 attaagagaa caatttccta accgcacaat agtctttaat caatcctcag gaggggaccc
     6061 agaaattaca atgcactctt ttaattgtgg aggggaattt ttctactgta atacagcgaa
     6121 actgtttaac agtacttggt tcaataacac tgaaggcata aataccactg aaggaaatgg
     6181 caatatcaca cgcaatatca cactccaatg cagaataaaa caatttataa acatgtggca
     6241 ggaagtagga aaagcaatgt atgcccctcc catcagtgga ccgattagct gtaaatcaaa
     6301 tattacaggg ttgatattaa caagagatgg tggtgaggat aacaacaccg agaccgagat
     6361 ttttagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     6421 agtagtaaga attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     6481 gagagaaaaa agagcagtgg ggctgggagc tttgttcctt gggttcttgg gagcagcagg
     6541 aagcactatg ggcgcagcgt cattgacgct gacggtacaa gccagacaat tattgtctgg
     6601 tatagtgcag cagcagaaca atctgctgag ggctattgag gcgcaacagc atctgttgca
     6661 actcactgtc tggggcatca agcagctcca ggcaagagtc ctggctgtgg aaagatacct
     6721 acaggatcaa cagctcctgg ggatttgggg ctgctctgga aaactcatct gcaccactac
     6781 tgtgccttgg aatactagct ggagtaataa atctttggaa caaatttgga atagcacgac
     6841 ttggatggag tgggataaag aagttagcaa atacacaggc ataatataca ccttaattga
     6901 agaatcgcag acccaacaag aaaagaatga acaagactta ttggcattag ataaatggga
     6961 cagtttgtgg aattggttta acatatcaaa ctggctgtgg tatataaaaa tattcataat
     7021 gatagtagga ggcttggtag gtttaagaat agtcttttct gtacttacta tagtaaatag
     7081 agttaggcag ggatattcac cattatcgtt tcagacccac cgcccagccc agaggggacc
     7141 agacaggccc gaaggaatcg aagacggagg tggagagaga gacagagaca gatcaccacc
     7201 attagtggat ggattcttga cacttatctg ggtcgacctg aggagcctgt tcctcttcac
     7261 ctaccaccgc ttgagagact tactcttgat tgtaacgagg attgtggaga ctctgagacg
     7321 cagggggtgg gaactcctca agtactggtg gaaccttcta caatattgga ttcaggaact
     7381 aaagaatagt gctgttagct tgctcaacgc cacagccata gcagtagctg aggggacaga
     7441 taggcttcta gaactattac aaagggctta tagagctatt cttaacatcc ctacaagaat
     7501 aagacagggc ttagaacgag ctttgttata agatgggtgg caagggatca aaaagttgtg
     7561 tgcctggatg gtctgctgta agggaaagaa tgagacgaac tgacccggca acagaaaggg
     7621 cggcagcaga aggggtggga gcagtatctc gagacggatc cagctaaggg tcaagacaat
     7681 tctgcagata tccagcacag tggcggccgc tcgagtctag agggcccgcg gttcgaaggt
     7741 aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtca tcatcaccat
     7801 caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     7861 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     7921 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     7981 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     8041 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     8101 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     8161 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     8221 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     8281 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     8341 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     8401 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     8461 ttataaggga ttttgccgat ttcggcctat tg
//
