LOCUS       sequence-269456-        5056 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Env gene from HIV patient plasma in pcDNA3.1 expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Joshi A, Sedano M, Beauchamp B, Punke EB, Mulla ZD, Meza A, Alozie 
            OK, Mukherjee D, Garg H
  TITLE     HIV-1 Env Glycoprotein Phenotype along with Immune Activation 
            Determines CD4 T Cell Loss in HIV Patients.
  JOURNAL   J Immunol. 2016 Feb 15;196(4):1768-79. doi: 
            10.4049/jimmunol.1501588. Epub 2016 Jan 13.
  PUBMED    26764036
REFERENCE   2  (bases 1 to 5056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.1501588"; journalName: "J Immunol"; date: 
            "2016-02-15- 15"; volume: "196"; issue: "4"; pages: "1768-79"
COMMENT     Sequence Label: pHP015Env
FEATURES             Location/Qualifiers
     source          1..5056
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        22..999
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        1034..1052
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1034..1051
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             1145..1192
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     regulatory      1284..1293
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2107..2241
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2131..2150)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2185..2204
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2354..2374)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2354..2372)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2393..2409)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2393..2409)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2406..2428)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2417..2433
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2441..2447,2448..2465,2466..2471))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2486..2507
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2624..2641)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2795..3383)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2875..2894)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3554..4345,4346..4414))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4177..4196
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4415..4519)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4545..5000)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4682..4703)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4894..4913
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tcgctattac gccagtcgac cgccaattca atatggcgta tatggactca tgccaattca
       61 atatggtgga tctggacctg tgccaattca atatggcgta tatggactcg tgccaattca
      121 atatggtgga tctggacccc agccaattca atatggcgga cttggcacca tgccaattca
      181 atatggcgga cctggcactg tgccaactgg ggaggggtct acttggcacg gtgccaagtt
      241 tgaggagggg tcttggccct gtgccaagtc cgccatattg aattggcatg gtgccaataa
      301 tggcggccat attggctata tgccaggatc aatatatagg caatatccaa tatggcccta
      361 tgccaatatg gctattggcc aggttcaata ctatgtattg gccctatgcc atatagtatt
      421 ccatatatgg gttttcctat tgacgtagat agcccctccc aatgggcggt cccatatacc
      481 atatatgggg cttcctaata ccgcccatag ccactccccc attgacgtca atggtctcta
      541 tatatggtct ttcctattga cgtcatatgg gcggtcctat tgacgtatat ggcgcctccc
      601 ccattgacgt caattacggt aaatggcccg cctggctcaa tgcccattga cgtcaatagg
      661 accacccacc attgacgtca atgggatggc tcattgccca ttcatatccg ttctcacgcc
      721 ccctattgac gtcaatgacg gtaaatggcc cacttggcag tacatcaata tctattaata
      781 gtaacttggc aagtacatta ctattggaag tacgccaggg tacattggca gtactcccat
      841 tgacgtcaat ggcggtaaat ggcccgcgat ggctgccaag tacatcccca ttgacgtcaa
      901 tggggagggg caatgacgca aatgggcgtt ccattgacgt aaatgggcgg taggcgtgcc
      961 taatgggagg tctatataag caatgctcgt ttagggaacc gccattctgc ctggggacgt
     1021 cggagcaagc ttgatttagg tgacactata gaatacaagc tacttgttct ttttgcagga
     1081 tccactagtg gccgccatta attaaaggcc ggccagcgat cgccggacat cgattcccac
     1141 catggactat aaggatgacg acgataagga ttacaaagac gatgatgaca aagggtccat
     1201 gtcggaggag cagttcggcg gggacggggc ggcggcagcg gcaacggcgg cggtaggcgg
     1261 ctcggcgggc gagcaggagg gagccatggt ggcggcgaca cagggggcag cggcggcggc
     1321 gggaagcgga gccgggaccg ggggcggaac cgcgtctgga ggcaccgaag ggggcagcgc
     1381 cgagtcggag ggggcgaaga ttgacgccag taagaacgag gaggatgaag ggaaaatgtt
     1441 tataggaggc cttagctggg acactacaaa gaaagatctg aaggactact tttccaaatt
     1501 tggtgaagtt gtagactgca ctctgaagtt agatcctatc acagggcgat caaggggttt
     1561 tggctttgtg ctatttaaag aatcggagag tgtagataag gtcatggatc aaaaagaaca
     1621 taaattgaat gggaaggtga ttgatcctaa aagggccaaa gccatgaaaa caaaagagcc
     1681 ggttaaaaaa atttttgttg gtggcctttc tccagataca cctgaagaga aaataaggga
     1741 gtactttggt ggttttggtg aggtggaatc catagagctc cccatggaca acaagaccaa
     1801 taagaggcgt gggttctgct ttattacctt taaggaagaa gaaccagtga agaagataat
     1861 ggaaaagaaa taccacaatg ttggtcttag taaatgtgaa ataaaagtag ccatgtcgaa
     1921 ggaacaatat cagcaacagc aacagtgggg atctagagga ggatttgcag gaagagctcg
     1981 tggaagaggt ggtgaccagc agagtggtta tgggaaggta tccaggcgag gtggtcatca
     2041 aaatagctac aaaccatact aaaaggcctc tcgagcctct agaactatag tgagtcgtat
     2101 tacgtagatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     2161 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     2221 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     2281 ggggaggtgt gggaggtttt ttaattcgcg gccgcggcgc caatgcattg ggcccggtac
     2341 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
     2401 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccgggagca
     2461 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     2521 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     2581 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     2641 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     2701 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     2761 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     2821 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     2881 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     2941 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3001 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3061 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3121 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3181 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     3241 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3301 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3361 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3421 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     3481 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     3541 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     3601 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     3661 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     3721 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     3781 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     3841 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     3901 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     3961 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     4021 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     4081 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     4141 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     4201 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     4261 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     4321 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     4381 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     4441 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     4501 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa
     4561 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     4621 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     4681 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     4741 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     4801 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     4861 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     4921 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     4981 tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg
     5041 atcggtgcgg gcctct
//
