LOCUS       sequence-269457-        6430 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  UPR signaling reporter (upregulates eYFP expression under UPR 
            conditions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6430)
  AUTHORS   Alcantara A, Seitner D, Navarrete F, Djamei A
  TITLE     A high-throughput screening method to identify proteins involved in 
            unfolded protein response of the endoplasmic reticulum in plants.
  JOURNAL   Plant Methods. 2020 Jan 21;16:4. doi: 10.1186/s13007-020-0552-3. 
            eCollection 2020.
  PUBMED    31988651
REFERENCE   2  (bases 1 to 6430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2020-01-21"; volume: "16:4. doi"; pages: " 
            10.1186/s13007-020-0552-3. eCollection 2020"
COMMENT     Sequence Label: pBIP1::eYFP
FEATURES             Location/Qualifiers
     source          1..6430
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    194..216
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     misc_feature    1512..1567
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             join(1575..1577,1578..1580,1581..2291)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1620..1641)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1881..1900)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2228..2249
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        3050..3229
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             3271..3822
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     misc_feature    4109..4133
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(4224..4812)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4304..4323)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4983..5771)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(6062..6430,1..67)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 ggacaatgcg ctacgcgcac cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt
       61 cgagcgcctt tgcccacaac ccggcggccg gccgcaacag atcgttttat aaattttttt
      121 ttttgaaaaa gaaaaagccc gaaaggcggc aacctctcgg gcttctggat ttccgatccc
      181 cggaattaga tcttggcagg atatattgtg gtgtaacgtt ctcgaactag gctcggacga
      241 agtaagcggc cgcaacctta attaaacacg gcgcgccaac agcgatcgca ccaggtacca
      301 cctagaggag gttgagagag aagatagaca atatatggca aatatctaag aaagtagtgt
      361 caatatgtta ttttgattta taatttttaa aatattgttg taatagttat taaaatgata
      421 tgttattaat tattaaaatt gttaaaattt gttttggttt tcggtacaca cttttgcata
      481 ctacagtacg tttttaacca ttcaaaggat aaattaaagt tagtacgcat gactccttac
      541 caagatgtta gtacgcatgt cttaggatag aacataccat tcaatcatta aaggctttaa
      601 tgggtcattt cacttgggct tctaaaattt aatatcggtt ctatttattt tagattagaa
      661 ctaattaagt taatctcatt cttcataata taatctcatt tatgaaagaa tatttttttt
      721 aaatgataac aaaataaata taaaacaaat actttttaaa aaatcaatag caaaaataga
      781 tagtacgttt ctctttgtta acaatgatgc ttaggggtag tagacattcg ggtacgcagg
      841 tcgggtttgg ataaaatccg atcgggtaag gatcctttgg gttgagaagt ttaggattca
      901 ataggataaa tctaaaatct aaatttgttt ccggtttggg tccaatcgga tttgagattt
      961 ttttactata tttttcagat ctttttttgg aactaatacc caaactattg agtaaatatc
     1021 tggatttttg ggtaaaattc ggaatacgga ttcgggttcg gattcgattt ttttggatag
     1081 aaaaatttag gatccaataa aataatttct cagcttttaa atttagattt gtatccaatt
     1141 tttcggatgg tttggattat tcgggttagg tttttcttcg cagccctagt gatactaata
     1201 gaagaagact gggcccaaca aaagctcatt gtctagaact aagaagaaga accgaagtaa
     1261 ccaacggcca cgattactcc aacacaagac caaatctgat tggttgacat tacaaatcgt
     1321 cgtaagataa ttggtccacg tcatctctga tgacatagtt aaatttcttc gtcttccata
     1381 aaaagcttct tcttcaaagc ttaaaaaaca aaaggaagct ctctctgtgt tcaaaacaag
     1441 ttcattcgat ctatagagac gaaacaaaaa gagagatcgt acgcaaaagt ttccgataac
     1501 atatttttac aacaattacc aacaacaaca aacaacaaac aacattacaa ttactattta
     1561 caattacagg ctctatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     1621 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     1681 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     1741 tgccctggcc caccctcgtg accaccttcg gctacggcct gcagtgcttc gcccgctacc
     1801 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     1861 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     1921 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     1981 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     2041 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     2101 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     2161 tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc
     2221 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     2281 agctgtacaa gtcaggtgga tcctagataa cctttacctt cattccctgt aaattggtac
     2341 cctgcatctc gtctctgtta tgcttaagaa gttcaatgtt tcgtttcatg taaaactttg
     2401 gtggtttgtg ttttggggcc ttgtataatc cctgatgaat aagtgttcta ctatgtttcc
     2461 gttcctgtta tctctttctt tctaatgaca agtcgaactt cttctttatc atcgcttcgt
     2521 ttttattatc tgtgcttctt ttgtttaata cgcctgcaaa gtgactcgac tctgtttagt
     2581 gcagttctgc gaaacttgta aatagtccaa ttgttggcct ctagtaatag atgtagcgaa
     2641 agtgttgagc tgttgggttc taaggatggc ttgaacatgt taatctttta ggttctgagt
     2701 atgatgaaca ttcgttgttg ctaagaaatg cctgtaatgt cccacaaatg tagaaaatgg
     2761 ttcgtacctt tgtccaagca ttgatatgtc tgatgagagg aaactgcaag atactgagct
     2821 tggtttaacg aaggagaggc agtttcttcc ttccaaagca tttcatttga caatgccttg
     2881 atcatcttaa gtagagtttc tgttgtggaa agtttgaaac tttgaagaaa cgactctcaa
     2941 gtaaattgat gatcacaagt gaaagtgtat gttactagag aattaaggga gtcacgttat
     3001 gacccccgcc gatgacgcgg gacaagccgt tttacgtttg gaactgacag aaccgcaacg
     3061 ttgaaggagc cactgagccg cgggtttctg gagtttaatg agctaagcac atacgtcaga
     3121 aaccattatt gcgcgttcaa aagtcgccta aggtcactat cagctagcaa atatttcttg
     3181 tcaaaaatgc tccactgacg ttccataaat tcccctcggt atccaattag agtctcatat
     3241 tcactctcaa ctcgatcgag gggatctacc atgagcccag aacgacgccc ggccgacatc
     3301 cgccgtgcca ccgaggcgga catgccggcg gtctgcacca tcgtcaacca ctacatcgag
     3361 acaagcacgg tcaacttccg taccgagccg caggaaccgc aggagtggac ggacgacctc
     3421 gtccgtctgc gggagcgcta tccctggctc gtcgccgagg tggacggcga ggtcgccggc
     3481 atcgcctacg cgggtccctg gaaggcacgc aacgcctacg actggacggc cgagtccacc
     3541 gtgtacgtct ccccccgcca tcagcggacg ggactgggct ccacgctcta cacccacctg
     3601 ctgaagtccc tggaggcaca gggcttcaag agcgtggtcg ctgtcatcgg gctgcccaac
     3661 gacccgagcg tgcgcatgca cgaggcgctc ggatatgccc cccgcggcat gctgcgggcg
     3721 gccggcttca agcacgggaa ctggcatgac gtgggtttct ggcagctgga cttcagcctg
     3781 ccggtgccgc cccgtccggt cctgcccgtc accgaaatct gatgacccct agagtcaagc
     3841 agatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     3901 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     3961 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4021 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa gtattctaga ccaagcatgc
     4081 acagctggcg ttctcagcca gatccccttg acaggatata ttggcgggta aactaagtcg
     4141 ctgtatgtgt ttgtttgaga tctcatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     4201 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     4261 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     4321 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4381 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4441 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4501 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4561 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4621 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     4681 tgcgctctgc tgaagccagt taccttcgga agaagagttg gtagctcttg atccggcaaa
     4741 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4801 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     4861 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     4921 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgtgtaaca ttggtctagt
     4981 gattatttgc cgactacctt ggtgatctcg cctttcacgt agtgaacaaa ttcttccaac
     5041 tgatctgcgc gcgaggccaa gcgatcttct tgtccaagat aagcctgcct agcttcaagt
     5101 atgacgggct gatactgggc cggcaggcgc tccattgccc agtcggcagc gacatccttc
     5161 ggcgcgattt tgccggttac tgcgctgtac caaatgcggg acaacgtaag cactacattt
     5221 cgctcatcgc cagcccagtc gggcggcgag ttccatagcg ttaaggtttc atttagcgcc
     5281 tcaaatagat cctgttcagg aaccggatca aagagttcct ccgccgctgg acctaccaag
     5341 gcaacgctat gttctcttgc ttttgtcagc aagatagcca gatcaatgtc gatcgtggct
     5401 ggctcgaaga tacctgcaag aatgtcattg cgctgccatt ctccaaattg cagttcgcgc
     5461 ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg
     5521 cggagaatct cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct
     5581 cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat atcactgtgt
     5641 ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa cgtcggttcg
     5701 agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc ggcgatcacc
     5761 gcttccctca taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat
     5821 gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtggctt
     5881 tgttgaataa atcgaacttt tgctgagttg aaggatcaga tcacgcatct tcccgacaac
     5941 gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca actggtccac ctacaacaaa
     6001 gctctcatca accgtggctc cctcactttc tggctggatg atggggcgat tcaggcgatc
     6061 cccatccaac agcccgccgt cgagcgggct tttttatccc cggaagcctg tggatagagg
     6121 gtagttatcc acgtgaaacc gctaatgccc cgcaaagcct tgattcacgg ggctttccgg
     6181 cccgctccaa aaactatcca cgtgaaatcg ctaatcaggg tacgtgaaat cgctaatcgg
     6241 agtacgtgaa atcgctaata aggtcacgtg aaatcgctaa tcaaaaaggc acgtgagaac
     6301 gctaatagcc ctttcagatc aacagcttgc aaacacccct cgctccggca agtagttaca
     6361 gcaagtagta tgttcaatta gcttttcaat tatgaatata tatatcaatt attggtcgcc
     6421 cttggcttgt
//
