LOCUS       sequence-269463-        6588 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6588)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 6588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-PDHA1-N-10
FEATURES             Location/Qualifiers
     source          1..6588
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        95..398
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        399..602
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     553..573
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1852..1861
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1858..3288
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     polyA_signal    3410..3531
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3447..3466)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3501..3520
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3538..3993)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3675..3696)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3887..3906
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4020..4124
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4126..4483
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4126..4317
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4149..4169)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4334..4469
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4518..5312
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4518..5312
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4572..4591)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5182..5201
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5500..5519)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5544..5591
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5920..6508
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6409..6428
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgattctgtg gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg
       61 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      121 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      181 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      241 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      301 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      361 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      421 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      481 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      541 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      601 ctggtttagt gaaccgtcag atccgctagc accgccacct ccgctagcac cgccaccatg
      661 aggaagatgc tcgccgccgt ctcccgcgtg ctgtctggcg cttctcagaa gccggcaagc
      721 agagtgctgg tagcatcccg taattttgca aatgatgcta catttgaaat taagaaatgt
      781 gaccttcacc ggctggaaga aggccctcct gtcacaacag tgctcaccag ggaggatggg
      841 ctcaaatact acaggatgat gcagactgta cgccgaatgg agttgaaagc agatcagctg
      901 tataaacaga aaattattcg tggtttctgt cacttgtgtg atggtcagga agcttgctgt
      961 gtgggcctgg aggccggcat caaccccaca gaccatctca tcacagccta ccgggctcac
     1021 ggctttactt tcacccgggg cctttccgtc cgagaaattc tcgcagagct tacaggacga
     1081 aaaggaggtt gtgctaaagg gaaaggagga tcgatgcaca tgtatgccaa gaacttctac
     1141 gggggcaatg gcatcgtggg agcgcaggtg cccctgggcg ctgggattgc tctagcctgt
     1201 aagtataatg gaaaagatga ggtctgcctg actttatatg gcgatggtgc tgctaaccag
     1261 ggccagatat tcgaagctta caacatggca gctttgtgga aattaccttg tattttcatc
     1321 tgtgagaata atcgctatgg aatgggaacg tctgttgaga gagcggcagc cagcactgat
     1381 tactacaaga gaggcgattt cattcctggg ctgagagtgg atggaatgga tatcctgtgc
     1441 gtccgagagg caacaaggtt tgctgctgcc tattgtagat ctgggaaggg gcccatcctg
     1501 atggagctgc agacttaccg ttaccacgga cacagtatga gtgaccctgg agtcagttac
     1561 cgtacacgag aagaaattca ggaagtaaga agtaagagtg accctattat gcttctcaag
     1621 gacaggatgg tgaacagcaa tcttgccagt gtggaagaac taaaggaaat tgatgtggaa
     1681 gtgaggaagg agattgagga tgctgcccag tttgccacgg ccgatcctga gccacctttg
     1741 gaagagctgg gctaccacat ctactccagc gacccacctt ttgaagttcg tggtgccaat
     1801 cagtggatca agtttaagtc agtcagtagc tccggagggg atccaccggt cgccaccatg
     1861 gtgagcaagg gcgaggaggt catcaaagag ttcatgcgct tcaaggtgcg catggagggc
     1921 tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     1981 acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
     2041 ctgtcccccc agttcatgta cggctccaag gcgtacgtga agcaccccgc cgacatcccc
     2101 gattacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     2161 gacggcggtc tggtgaccgt gacccaggac tcctccctgc aggacggcac gctgatctac
     2221 aaggtgaaga tgcgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc
     2281 atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag
     2341 atccaccagg ccctgaagct gaaggacggc ggccactacc tggtggagtt caagaccatc
     2401 tacatggcca agaagcccgt gcaactgccc ggctactact acgtggacac caagctggac
     2461 atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc cgagggccgc
     2521 caccacctgt tcctggggca tggcaccggc agcaccggca gcggcagctc cggcaccgcc
     2581 tcctccgagg acaacaacat ggccgtcatc aaagagttca tgcgcttcaa ggtgcgcatg
     2641 gagggctcca tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     2701 gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg
     2761 gacatcctgt ccccccagtt catgtacggc tccaaggcgt acgtgaagca ccccgccgac
     2821 atccccgatt acaagaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     2881 ttcgaggacg gcggtctggt gaccgtgacc caggactcct ccctgcagga cggcacgctg
     2941 atctacaagg tgaagatgcg cggcaccaac ttcccccccg acggccccgt aatgcagaag
     3001 aagaccatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag
     3061 ggcgagatcc accaggccct gaagctgaag gacggcggcc actacctggt ggagttcaag
     3121 accatctaca tggccaagaa gcccgtgcaa ctgcccggct actactacgt ggacaccaag
     3181 ctggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga gcgctccgag
     3241 ggccgccacc acctgttcct gtacggcatg gacgagctgt acaagtaagc ggccgcgact
     3301 ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     3361 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     3421 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     3481 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa
     3541 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     3601 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     3661 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     3721 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     3781 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     3841 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     3901 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     3961 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc
     4021 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     4081 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa
     4141 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     4201 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     4261 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     4321 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     4381 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     4441 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag
     4501 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     4561 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     4621 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     4681 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     4741 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     4801 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     4861 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     4921 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     4981 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     5041 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     5101 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     5161 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     5221 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     5281 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     5341 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     5401 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     5461 catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat
     5521 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg
     5581 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     5641 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
     5701 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc
     5761 aggttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     5821 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     5881 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     5941 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     6001 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     6061 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     6121 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     6181 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     6241 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     6301 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     6361 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     6421 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     6481 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     6541 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccc
//
