LOCUS       sequence-269469-       12563 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of mCherry-LacR-BRCA1 siRNA resistant 
            construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12563)
  AUTHORS   Orthwein A, Noordermeer SM, Wilson MD, Landry S, Enchev RI, Sherker 
            A, Munro M, Pinder J, Salsman J, Dellaire G, Xia B, Peter M, 
            Durocher D
  TITLE     A mechanism for the suppression of homologous recombination in G1 
            cells.
  JOURNAL   Nature. 2015 Dec 9. doi: 10.1038/nature16142.
  PUBMED    26649820
REFERENCE   2  (bases 1 to 12563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Dec 9. doi: 10.1038/nature16142."
COMMENT     Sequence Label: pDEST-mCherry-LacR-BRCA1
FEATURES             Location/Qualifiers
     source          1..12563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        153..532
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        533..736
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     687..707
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    738..756
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    759..777
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     787..811
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             902..1609
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1047..1065)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1315..1334
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(1653..1672)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             2714..2734
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    2744..2768
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8367..8391)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(8433..8450)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8439..8663
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8796..8815)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    8947..8994
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             9002..10024
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    10154..10275
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10191..10210)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10245..10264
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10324..10340)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10324..10340)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10337..10359)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10348..10364
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10372..10378,10379..10396,10397..10402))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10417..10438
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10555..10572)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10726..11314)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10806..10825)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11485..12276,12277..12345))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12108..12127
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12346..12450)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12525..12544)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
       61 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
      121 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
      181 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
      241 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      301 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      361 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
      421 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
      481 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      541 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      601 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
      661 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
      721 ctatataagc agagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg
      781 tcgacgagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
      841 cctccataga agacaccggg accgatccag cctccggact ctagcgttta aacttaagct
      901 tatggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
      961 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     1021 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     1081 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     1141 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     1201 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     1261 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     1321 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     1381 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     1441 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     1501 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     1561 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt ccggactcag
     1621 atctcgaggg atggtgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc
     1681 ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga
     1741 aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact
     1801 ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc
     1861 gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt
     1921 gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc
     1981 gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt
     2041 ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat
     2101 caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc
     2161 attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct
     2221 gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg
     2281 ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg
     2341 catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc
     2401 cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac
     2461 cgaagacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct
     2521 ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa
     2581 tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac
     2641 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgaag
     2701 cagcctgagg cctcctaaga agaagaggaa ggttggcgcg cccacaagtt tgtacaaaaa
     2761 agcaggcttc accatggatt tatctgctct tcgcgttgaa gaagtacaaa atgtcattaa
     2821 tgctatgcag aaaatcttag agtgtcccat ctgtctggag ttgatcaagg aacctgtctc
     2881 cacaaagtgt gaccacatat tttgcaaatt ttgcatgctg aaacttctca accagaagaa
     2941 agggccttca cagtgtcctt tatgtaagaa tgatataacc aaaaggagcc tacaagaaag
     3001 tacgagattt agtcaacttg ttgaagagct attgaaaatc atttgtgctt ttcagcttga
     3061 cacaggtttg gagtatgcaa acagctataa ttttgcaaaa aaggaaaata actctcctga
     3121 acatctaaaa gatgaagttt ctatcatcca aagtatgggc tacagaaacc gtgccaaaag
     3181 acttctacag agtgaacccg aaaatccttc cttgcaggaa accagtctca gtgtccaact
     3241 ctctaacctt ggaactgtga gaactctgag gacaaagcag cggatacaac ctcaaaagac
     3301 gtctgtctac attgaattgg gatctgattc ttctgaagat accgttaata aggcaactta
     3361 ttgcagtgtg ggagatcaag aattgttaca aatcacccct caaggaacca gggatgaaat
     3421 cagtttggat tctgcaaaaa aggctgcttg tgaattttct gagacggatg taacaaatac
     3481 tgaacatcat caacccagta ataatgattt gaacaccact gagaagcgtg cagctgagag
     3541 gcatccagaa aagtatcagg gtagttctgt ttcaaacttg catgtggagc catgtggcac
     3601 aaatactcat gccagctcat tacagcatga gaacagcagt ttattactca ctaaagacag
     3661 aatgaatgta gaaaaggctg aattctgtaa taaaagcaaa cagcctggct tagcaaggag
     3721 ccaacataac agatgggctg gaagtaagga aacatgtaat gataggcgga ctcccagcac
     3781 agaaaaaaag gtagatctga atgctgatcc cctgtgtgag agaaaagaat ggaataagca
     3841 gaaactgcca tgctcagaga atcctagaga tactgaagat gttccttgga taacactaaa
     3901 tagcagcatt cagaaagtta atgagtggtt ttccagaagt gatgaactgt taggttctga
     3961 tgactcacat gatggggagt ctgaatcaaa tgccaaagta gctgatgtat tggacgttct
     4021 aaatgaggta gatgaatatt ctggttcttc agagaaaata gacttactgg ccagtgatcc
     4081 tcatgaggct ttaatatgta aaagtgaaag agttcactcc aaatcagtag agagtaatat
     4141 tgaagacaaa atatttggga aaacctatcg gaagaaggca agcctcccca acttaagcca
     4201 tgtaactgaa aatctaatta taggagcatt tgttactgag ccacagataa tacaagagcg
     4261 tcccctcaca aataaattaa agcgtaaaag gagacctaca tcaggccttc atcctgagga
     4321 ttttatcaag aaagcagatt tggcagttca aaagactcct gaaatgataa atcagggaac
     4381 taaccaaacg gagcagaatg gtcaagtgat gaatattact aatagtggtc atgagaataa
     4441 aacaaaaggt gattctattc agaatgagaa aaatcctaac ccaatagaat cactcgaaaa
     4501 agaatctgct ttcaaaacga aagctgaacc tataagcagc agtataagca atatggaact
     4561 cgaattaaat atccacaatt caaaagcacc taaaaagaat aggctgagga ggaagtcttc
     4621 taccaggcat attcatgcgc ttgaactagt agtcagtaga aatctaagcc cacctaattg
     4681 tactgaattg caaattgata gttgttctag cagtgaagag ataaagaaaa aaaagtacaa
     4741 ccaaatgcca gtcaggcaca gcagaaacct acaactcatg gaaggtaaag aacctgcaac
     4801 tggagccaag aagagtaaca agccaaatga acagacaagt aaaagacatg acagtgatac
     4861 tttcccagag ctgaagttaa caaatgcacc tggttctttt actaagtgtt caaataccag
     4921 tgaacttaaa gaatttgtca atcctagcct tccaagagaa gaaaaagaag agaaactaga
     4981 aacagttaaa gtgtctaata atgctgaaga ccccaaagat ctcatgttaa gtggagaaag
     5041 ggttttgcaa actgaaagat ctgtagagag tagcagtatt tcactggtac ctggtactga
     5101 ttatggcact caggaaagta tctcgttact ggaagttagc actctaggga aggcaaaaac
     5161 agaaccaaat aaatgtgtga gtcagtgtgc agcatttgaa aaccccaagg gactaattca
     5221 tggttgttcc aaagataata gaaatgacac agaaggcttt aagtatccat tgggacatga
     5281 agttaaccac agtcgggaaa caagcataga aatggaagaa agtgaacttg atgctcagta
     5341 tttgcagaat acattcaagg tttcaaagcg ccagtcattt gctctgtttt caaatccagg
     5401 aaatgcagaa gaggaatgtg caacattctc tgcccactct gggtccttaa agaaacaaag
     5461 tccaaaagtc acttttgaat gtgaacaaaa ggaagaaaat caaggaaaga atgagtctaa
     5521 tatcaagcct gtacagacag ttaatatcac tgcaggcttt cctgtggttg gtcagaaaga
     5581 taagccagtt gataatgcca aatgtagtat caaaggaggc tctaggtttt gtctatcatc
     5641 tcagttcaga ggcaacgaaa ctggactcat tactccaaat aaacatggac ttttacaaaa
     5701 cccatatcgt ataccaccac tttttcccat caagtcattt gttaaaacta aatgtaagaa
     5761 aaatctgcta gaggaaaact ttgaggaaca ttcaatgtca cctgaaagag aaatgggaaa
     5821 tgagaacatt ccaagtacag tgagcacaat tagccgtaat aacattagag aaaatgtttt
     5881 taaaggagcc agctcaagca atattaatga agtaggttcc agtactaatg aagtgggctc
     5941 cagtattaat gaaataggtt ccagtgatga aaacattcaa gcagaactag gtagaaacag
     6001 agggccaaaa ttgaatgcta tgcttagatt aggggttttg caacctgagg tctataaaca
     6061 aagtcttcct ggaagtaatt gtaagcatcc tgaaataaaa aagcaagaat atgaagaagt
     6121 agttcagact gttaatacag atttctctcc atatctgatt tcagataact tagaacagcc
     6181 tatgggaagt agtcatacat ctcaggtttg ttctgagaca cctgatgacc tgttagatga
     6241 tggtgaaata aaggaagata ctagttttgc tgaaaatgac attaaggaaa gttctgctgt
     6301 ttttagcaaa agcgtccaga gaggagagct tagcaggagt cctagccctt tcacccatac
     6361 acatttggct cagggttacc gaagaggggc caagaaatta gagtcctcag aagagaactt
     6421 atctagtgag gatgaagagc ttccctgctt ccaacacttg ttatttggta aagtaaacaa
     6481 tataccttct cagtctacta ggcatagcac cgttgctacc gagtgtctgt ctaagaacac
     6541 agaggagaat ttattatcat tgaagaatag cttaaatgac tgcagtaacc aggtaatatt
     6601 ggcaaaggca tctcaggaac atcaccttag tgaggaaaca aaatgttctg ctagcttgtt
     6661 ttcttcacag tgcagtgaat tggaagactt gactgcaaat acaaacaccc aggatccttt
     6721 cttgattggt tcttccaaac aaatgaggca tcagtctgaa agccagggag ttggtctgag
     6781 tgacaaggaa ttggtttcag atgatgaaga aagaggaacg ggcttggaag aaaataatca
     6841 agaagagcaa agcatggatt caaacttagg tgaagcagca tctgggtgtg agagtgaaac
     6901 aagcgtctct gaagactgct cagggctatc ctctcagagt gacattttaa ccactcagca
     6961 gagggatacc atgcaacata acctgataaa gctccagcag gaaatggctg aactagaagc
     7021 tgtgttagaa cagcatggga gccagccttc taacagctac cctagtataa tcagtgactc
     7081 ttctgccctt gaggacctgc gaaatccaga acaaagcaca tcagaaaaag cagtattaac
     7141 ttcacagaaa agtagtgaat accctataag ccagaatcca gaaggccttt ctgctgacaa
     7201 gtttgaggtg tctgcagata gttctaccag taaaaataaa gaaccaggag tggaaaggtc
     7261 atccccttct aaatgcccat cattagatga taggtggtac atgcacagtt gctctgggag
     7321 tcttcagaat agaaactacc catctcaaga ggagctcatt aaggttgttg atgtggagga
     7381 gcaacagctg gaagagtctg ggccacacga tttgacggaa acatcttact tgccaaggca
     7441 agatctagag ggaacccctt acctggaatc tggaatcagc ctcttctctg atgaccctga
     7501 atctgatcct tctgaagaca gagccccaga gtcagctcgt gttggcaaca taccatcttc
     7561 aacctctgca ttgaaagttc cccaattgaa agttgcagaa tctgcccagg gtccagctgc
     7621 tgctcatact actgatactg ctgggtataa tgcaatggaa gaaagtgtga gcagggagaa
     7681 gccagaattg acagcttcaa cagaaagggt caacaaaaga atgtccatgg tggtgtctgg
     7741 cctgacccca gaagaattta tgctcgtgta caagtttgcc agaaaacacc acatcacttt
     7801 aactaatcta attactgaag agactactca tgttgttatg aaaacagatg ctgagtttgt
     7861 gtgtgaacgg acactgaaat attttctagg aattgcggga ggaaaatggg tagttagcta
     7921 tttctgggtg acccagtcta ttaaagaaag aaaaatgctg aatgagcatg attttgaagt
     7981 cagaggagat gtggtcaatg gaagaaacca ccaaggtcca aagcgagcaa gagaatccca
     8041 ggacagaaag atcttcaggg ggctagaaat ctgttgctat gggcccttca ccaacatgcc
     8101 cacagatcaa ctggaatgga tggtacagct gtgtggtgct tctgtggtga aggagctttc
     8161 atcattcacc cttggcacag gtgtccaccc aattgtggtt gtgcagccag atgcctggac
     8221 agaggacaat ggcttccatg caattgggca gatgtgtgag gcacctgtgg tgacccgaga
     8281 gtgggtgttg gacagtgtag cactctacca gtgccaggag ctggacacct acctgatacc
     8341 ccagatcccc cacagccact actgagaccc agctttcttg tacaaagtgg tgtgactcga
     8401 gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     8461 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     8521 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     8581 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     8641 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     8701 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     8761 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     8821 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     8881 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     8941 tacctagaag ttcctattcc gaagttccta ttctctagaa agtataggaa cttccttggc
     9001 caaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
     9061 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
     9121 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
     9181 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
     9241 ggagttcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca
     9301 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc
     9361 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
     9421 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
     9481 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
     9541 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc
     9601 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
     9661 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg
     9721 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg
     9781 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
     9841 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
     9901 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
     9961 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga
    10021 atagcacgta ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
    10081 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
    10141 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
    10201 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    10261 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
    10321 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
    10381 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
    10441 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
    10501 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
    10561 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
    10621 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
    10681 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
    10741 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
    10801 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    10861 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    10921 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    10981 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    11041 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    11101 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    11161 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    11221 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    11281 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    11341 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    11401 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    11461 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    11521 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    11581 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    11641 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    11701 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    11761 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    11821 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    11881 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    11941 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    12001 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    12061 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    12121 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    12181 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    12241 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    12301 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    12361 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    12421 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
    12481 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
    12541 gccgcatagt taagccagta tct
//
