LOCUS       sequence-269485-       15309 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15309)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15309)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX061
FEATURES             Location/Qualifiers
     source          1..15309
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      211..922
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(1049..1420)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            1453..1562
                     /note="incP origin of transfer"
     primer_bind     1785..1804
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2038..2055
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(2243..2893)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    3358..3382
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        3481..3826
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    4453..4629
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        5132..5477
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    5499..5523
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6377..6401)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6430..6519
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6553..6805
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(7234..7486)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7954..8979)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7954..8979)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8989..9333)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9443..9460)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9443..9459)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9452..9474)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9599..9618)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9809..9830
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    10077..10101
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11697..12845)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13144..13938)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLLLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14130..14897
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
ORIGIN
        1 ttgccggccc cgccatgaag tccgtgaatg ccccgacggc cgaagtgaag ggcaggccgc
       61 cacccaggcc gccgccctca ctgcccggca cctggtcgct gaatgtcgat gccagcacct
      121 gcggcacgtc aatgcttccg ggcgtcgcgc tcgggctgat cgcccatccc gttactgccc
      181 cgatcccggc aatggcaagg actgccagcg ctgccatttt tggggtgagg ccgttcgcgg
      241 ccgaggggcg cagcccctgg ggggatggga ggcccgcgtt agcgggccgg gagggttcga
      301 gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta
      361 aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa agacaggtta gcggtggccg
      421 aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc ctgtggacag cccctcaaat
      481 gtcaataggt gcgcccctca tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc
      541 cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc gcagggcact tatccccagg
      601 cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag
      661 gctggccagc tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt caacgccgcg
      721 ccgggtgagt cggcccctca agtgtcaacg tccgcccctc atctgtcagt gagggccaag
      781 ttttccgcga ggtatccaca acgccggcgg ccgcggtgtc tcgcacacgg cttcgacggc
      841 gtttctggcg cgtttgcagg gccatagacg gccgccagcc cagcggcgag ggcaaccagc
      901 ccggtgagcg tcggaaaggc gctcggtctt gccttgctcg tcggtgatgt acttcaccag
      961 ctccgcgaag tcgctcttct tgatggagcg catggggacg tgcttggcaa tcacgcgcac
     1021 cccccggccg ttttagcggc taaaaaagtc atggctctgc cctcgggcgg accacgccca
     1081 tcatgacctt gccaagctcg tcctgcttct cttcgatctt cgccagcagg gcgaggatcg
     1141 tggcatcacc gaaccgcgcc gtgcgcgggt cgtcggtgag ccagagtttc agcaggccgc
     1201 ccaggcggcc caggtcgcca ttgatgcggg ccagctcgcg gacgtgctca tagtccacga
     1261 cgcccgtgat tttgtagccc tggccgacgg ccagcaggta ggccgacagg ctcatgccgg
     1321 ccgccgccgc cttttcctca atcgctcttc gttcgtctgg aaggcagtac accttgatag
     1381 gtgggctgcc cttcctggtt ggcttggttt catcagccat ccgcttgccc tcatctgtta
     1441 cgccggcggt agccggccag cctcgcagag caggattccc gttgagcacc gccaggtgcg
     1501 aataagggac agtgaagaag gaacacccgc tcgcgggtgg gcctacttca cctatcctgc
     1561 ccggctgacg ccgttggata caccaaggaa agtctacacg aaccctttgg caaaatcctg
     1621 tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc tataatgacc ccgaagcagg
     1681 gttatgcagc ggaaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1741 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     1801 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     1861 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     1921 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     1981 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     2041 gagtcagtga gcgaggaagc ggaagagcgc cagaaggccg ccagagaggc cgagcgcggc
     2101 cgtgaggctt ggacgctagg gcagggcatg aaaaagcccg tagcgggctg ctacgggcgt
     2161 ctgacgcggt ggaaaggggg aggggatgtt gtctacatgg ctctgctgta gtgagtgggt
     2221 tgcgctccgg cagcggtcct gatcaatcgt caccctttct cggtccttca acgttcctga
     2281 caacgagcct ccttttcgcc aatccatcga caatcaccgc gagtccctgc tcgaacgctg
     2341 cgtccggacc ggcttcgtcg aaggcgtcta tcgcggcccg caacagcggc gagagcggag
     2401 cctgttcaac ggtgccgccg cgctcgccgg catcgctgtc gccggcctgc tcctcaagca
     2461 cggccccaac agtgaagtag ctgattgtca tcagcgcatt gacggcgtcc ccggccgaaa
     2521 aacccgcctc gcagaggaag cgaagctgcg cgtcggccgt ttccatctgc ggtgcgcccg
     2581 gtcgcgtgcc ggcatggatg cgcgcgccat cgcggtaggc gagcagcgcc tgcctgaagc
     2641 tgcgggcatt cccgatcaga aatgagcgcc agtcgtcgtc ggctctcggc accgaatgcg
     2701 tatgattctc cgccagcatg gcttcggcca gtgcgtcgag cagcgcccgc ttgttcctga
     2761 agtgccagta aagcgccggc tgctgaaccc ccaaccgttc cgccagtttg cgtgtcgtca
     2821 gaccgtctac gccgacctcg ttcaacaggt ccagggcggc acggatcact gtattcggct
     2881 gcaactttgt catgcttgac actttatcac tgataaacat aatatgtcca ccaacttatc
     2941 agtgataaag aatccgcgcg ttcaatcgga ccagcggagg ctggtccgga ggccagacgt
     3001 gaaacccaac atacccctga tcgtaattct gagcactgtc gcgctcgacg ctgtcggcat
     3061 cggcctgatt atgccggtgc tgccgggcct cctgcgcgat ctggttcact cgaacgacgt
     3121 caccgcccac tatggcattc tgctggcgct gtatgcgttg gtgcaatttg cctgcgcacc
     3181 tgtgctgggc gcgctgtcgg atcgtttcgg gcggcggcca atcttgctcg tctcgctggc
     3241 cggcgccaga tctggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa
     3301 accttttcac gcccttttaa atatccgatt attctaataa acgctctttt ctcttaggtt
     3361 tacccgccaa tatatcctgt caaacactga tagtttaaac gttgcgatta agaattcgag
     3421 ctcggtaccc ccctactcca aaaatgtcaa agatacagtc tcagaagacc aaagggctat
     3481 tgagactttt caacaaaggg taatttcggg aaacctcctc ggattccatt gcccagctat
     3541 ctgtcacttc atcgaaagga cagtagaaaa ggaaggtggc tcctacaaat gccatcattg
     3601 cgataaagga aaggctatca ttcaagatgc ctctgccgac agtggtccca aagatggacc
     3661 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     3721 ggattgatgt gacatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
     3781 agacccttcc tctatataag gaagttcatt tcatttggag aggacagccc aagcttcgac
     3841 tctagaggat ccccttaaat cgatatggaa cgagctatac aaggaaacga cgctagggaa
     3901 caagctaaca gtgaacgttg ggatggagga tcaggaggta ccacttctcc cttcaaactt
     3961 cctgacgaaa gtccgagttg gactgagtgg cggctacata acgatgagac gaattcgaat
     4021 caagataatc cccttggttt caaggaaagc tggggtttcg ggaaagttgt atttaagaga
     4081 tatctcagat acgacaggac ggaagcttca ctgcacagag tccttggatc ttggacggga
     4141 gattcggtta actatgcagc atctcgattt ttcggtttcg accagatcgg atgtacctat
     4201 agtattcggt ttcgaggagt tagtatcacc gtttctggag ggtctcgaac tcttcagcat
     4261 ctctgtgaga tggcaattcg gtctaagcaa gaactgctac agcttgcccc aatcgaagtg
     4321 gaaagtaatg tatcaagagg atgccctgaa ggtactcaaa ccttcgaaaa agaaagcgag
     4381 taagtcgagg gcgagctcga attcggtacg ctgaaatcac cagtctctct ctacaaatct
     4441 atctctctct attttctcca taaataatgt gtgagtagtt tcccgataag ggaaattagg
     4501 gttcttatag ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta
     4561 tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc cagtactaaa
     4621 atccagatct ccctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
     4681 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
     4741 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
     4801 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
     4861 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
     4921 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
     4981 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
     5041 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
     5101 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
     5161 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
     5221 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
     5281 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     5341 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     5401 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     5461 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagcag
     5521 gctccaccac tagtatggag ttgattgaag caagagcatt aaaatccagt tttctttcag
     5581 acatggccat gaaaactagc caacaagttt ttcttgatga tatatggtgt gtaacgggta
     5641 ttaataacgt accaagtgat gatttttcag ttgatgacct tttggatttt tctgacaaag
     5701 atttcaaaga tggccagtct ttacaagaat tgcatgaaga tgatgaaaaa gactcttttt
     5761 ctggctcttc acagcacaga aattctcaag tttcgaactt ttcatgcatg gatagttttt
     5821 ccggcgaact tcctgttccg gttgatgaat tggagaatct tgagtggtta tcacaatttg
     5881 tggatgattc cacgtcggac ttttccttgt tatgtcctgc cggccgtttt aaggacaaaa
     5941 ccggtggttt tcaagtttcc cgatctgaac cggttgttag accggttttt cagaaactga
     6001 gagttccatg ttttcctctt ccggttgttc agaaaccgag aaccaaacgc tccagaccgg
     6061 ctggaagaaa atggtctttt tcgtcaccaa cagtttcagc agactcgtgt tcacctacct
     6121 cttcatcatt gtcaggtcca aaaaacaccc cagtggcgag ccggtccatt gggtccaaaa
     6181 actttgtgca acgcttgtgg tgttagattc aaatccggtc ggctttttcc ggaatataga
     6241 ccagcttgta gtccgacttt ttctcaggaa gttcactcga atagtcaccg gaaagtgtta
     6301 gagatgcggc ggaagaagga gacaggtgat attgttgact ccggtctagc tactatgatt
     6361 tcaacttgtc ccggggaccc agctttcttg tacaaagtgg ttgataacag cgggttaatt
     6421 aacatctttt acccatacga tgttcctgac tatgcgggct atccctatga cgtcccggac
     6481 tatgcaggat cctatccata tgacgttcca gattacgctg ctcagtgcag cgcttagagc
     6541 tcgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
     6601 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt
     6661 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
     6721 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
     6781 gcggtgtcat ctatgttact agatcgggaa ttagcttcat caacgcaaga catgcgcacg
     6841 accgtctgac aggagaggaa tttccgacga gcacagaaag gacttgctct tggacgtagg
     6901 cctatttctc aggcacatgt atcaagtgtt cggacgtggg ttttcgatgg tgtatcagcc
     6961 gccgccaact gggagatgag gaggctttct tggggggcag tcagcagttc atttcacaag
     7021 acagaggaac ttgtaaggag atgcactgat ttatcttggc gcaaaccagc aggacgaatt
     7081 agtgggaata gcccgcgaat atctaagtta tgcctgtcgg catgagcaga aacttccaat
     7141 tcgaaacagt ttggagaggt tgtttttggg catacctttt gttagtcagc ctctcgattg
     7201 ctcatcgtca ttacacagta ccgaagtttg atcgatctag taacatagat gacaccgcgc
     7261 gcgataattt atcctagttt gcgcgctata ttttgttttc tatcgcgtat taaatgtata
     7321 attgcgggac tctaatcata aaaacccatc tcataaataa cgtcatgcat tacatgttaa
     7381 ttattacatg cttaacgtaa ttcaacagaa attatatgat aatcatcgca agaccggcaa
     7441 caggattcaa tcttaagaaa ctttattgcc aaatgtttga acgatctgct tcgacgcact
     7501 ccttctttac tccaccatct cgtccttatt gaaaacgtgg gtagcaccaa aacgaatcaa
     7561 gtcgctggaa ctgaagttac caatcacgct ggatgatttg ccagttggat taatcttgcc
     7621 tttccccgca tgaataatat tgatgaatgc atgcgtgagg ggtatttcga ttttggcaat
     7681 agctgcaatt gccgcgacat cctccaacga gcataattct tcagaaaaat agcgatgttc
     7741 catgttgtca gggcatgcat gatgcacgtt atgaggtgac ggtgctaggc agtattccct
     7801 caaagtttca tagtcagtat catattcatc attgcattcc tgcaagagag aattgagacg
     7861 caatccacac gctgcggcaa ccttccggcg ttcgtggtct atttgctctt ggacgttgca
     7921 aacgtaagtg ttggatcccg gtcggcatct actctattcc tttgccctcg gacgagtgct
     7981 ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc agacggccgc
     8041 gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga cgattgcgtc
     8101 gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc tctgatagag
     8161 ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg caagctccgg
     8221 atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa
     8281 gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc agtcaatgac
     8341 cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg cgtgcacgag
     8401 gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag cctgcgcgac
     8461 ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg gatcagcaat
     8521 cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc
     8581 gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatggcct ccgcgaccgg
     8641 ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac cctgtgcacg
     8701 gcgggagatg caataggtca ggctctcgct gaattcccca atgtcaagca cttccggaat
     8761 cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga aaccatcggc
     8821 gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga
     8881 ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa
     8941 cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tcggggtcgt cctctccaaa
     9001 tgaaatgaac ttccttatat agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca
     9061 tcccttacgt cagtggagat atcacatcaa tccacttgct ttgaagacgt ggttggaacg
     9121 tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca
     9181 gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct
     9241 tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg
     9301 tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta
     9361 tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttgac ggatctctag
     9421 gacgcgtcct agaagctaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     9481 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     9541 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgcccgct
     9601 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     9661 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     9721 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     9781 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     9841 aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc ggaaccacca
     9901 tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc
     9961 agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca
    10021 ccccagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt
    10081 acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca
    10141 aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt
    10201 gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg
    10261 ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga
    10321 gcgttgctgc ctgtgatcaa atatcatctc cctcgcagag atccgaatta tcagccttct
    10381 tattcatttc tcgcttaacc gtgacaggct gtcgatcttg agaactatgc cgacataata
    10441 ggaaatcgct ggataaagcc gctgaggaag ctgagtggcg ctatttcttt agaagtgaac
    10501 gttgacgata tcaactcccc tatccattgc tcaccgaatg gtacaggtcg gggacccgaa
    10561 gttccgactg tcggcctgat gcatccccgg ctgatcgacc ccagatctgg ggctgagaaa
    10621 gcccagtaag gaaacaactg taggttcgag tcgcgagatc ccccggaacc aaaggaagta
    10681 ggttaaaccc gctccgatca ggccgagcca cgccaggccg agaacattgg ttcctgtagg
    10741 catcgggatt ggcggatcaa acactaaagc tactggaacg agcagaagtc ctccggccgc
    10801 cagttgccag gcggtaaagg tgagcagagg cacgggaggt tgccacttgc gggtcagcac
    10861 ggttccgaac gccatggaaa ccgcccccgc caggcccgct gcgacgccga caggatctag
    10921 cgctgcgttt ggtgtcaaca ccaacagcgc cacgcccgca gttccgcaaa tagcccccag
    10981 gaccgccatc aatcgtatcg ggctacctag cagagcggca gagatgaaca cgaccatcag
    11041 cggctgcaca gcgcctaccg tcgccgcgac cccgcccggc aggcggtaga ccgaaataaa
    11101 caacaagctc cagaatagcg aaatattaag tgcgccgagg atgaagatgc gcatccacca
    11161 gattcccgtt ggaatctgtc ggacgatcat cacgagcaat aaacccgccg gcaacgcccg
    11221 cagcagcata ccggcgaccc ctcggcctcg ctgttcgggc tccacgaaaa cgccggacag
    11281 atgcgccttg tgagcgtcct tggggccgtc ctcctgtttg aagaccgaca gcccaatgat
    11341 ctcgccgtcg atgtaggcgc cgaatgccac ggcatctcgc aaccgttcag cgaacgcctc
    11401 catgggcttt ttctcctcgt gctcgtaaac ggacccgaac atctctggag ctttcttcag
    11461 ggccgacaat cggatctcgc ggaaatcctg cacgtcggcc gctccaagcc gtcgaatctg
    11521 agccttaatc acaattgtca attttaatcc tctgtttatc ggcagttcgt agagcgcgcc
    11581 gtgcgtcccg agcgatactg agcgaagcaa gtgcgtcgag cagtgcccgc ttgttcctga
    11641 aatgccagta aagcgctggc tgctgaaccc ccagccggaa ctgaccccac aaggccctag
    11701 cgtttgcaat gcaccaggtc atcattgacc caggcgtgtt ccaccaggcc gctgcctcgc
    11761 aactcttcgc aggcttcgcc gacctgctcg cgccacttct tcacgcgggt ggaatccgat
    11821 ccgcacatga ggcggaaggt ttccagcttg agcgggtacg gctcccggtg cgagctgaaa
    11881 tagtcgaaca tccgtcgggc cgtcggcgac agcttgcggt acttctccca tatgaatttc
    11941 gtgtagtggt cgccagcaaa cagcacgacg atttcctcgt cgatcaggac ctggcaacgg
    12001 gacgttttct tgccacggtc caggacgcgg aagcggtgca gcagcgacac cgattccagg
    12061 tgcccaacgc ggtcggacgt gaagcccatc gccgtcgcct gtaggcgcga caggcattcc
    12121 tcggccttcg tgtaataccg gccattgatc gaccagccca ggtcctggca aagctcgtag
    12181 aacgtgaagg tgatcggctc gccgataggg gtgcgcttcg cgtactccaa cacctgctgc
    12241 cacaccagtt cgtcatcgtc ggcccgcagc tcgacgccgg tgtaggtgat cttcacgtcc
    12301 ttgttgacgt ggaaaatgac cttgttttgc agcgcctcgc gcgggatttt cttgttgcgc
    12361 gtggtgaaca gggcagagcg ggccgtgtcg tttggcatcg ctcgcatcgt gtccggccac
    12421 ggcgcaatat cgaacaagga aagctgcatt tccttgatct gctgcttcgt gtgtttcagc
    12481 aacgcggcct gcttggcctc gctgacctgt tttgccaggt cctcgccggc ggtttttcgc
    12541 ttcttggtcg tcatagttcc tcgcgtgtcg atggtcatcg acttcgccaa acctgccgcc
    12601 tcctgttcga gacgacgcga acgctccacg gcggccgatg gcgcgggcag ggcaggggga
    12661 gccagttgca cgctgtcgcg ctcgatcttg gccgtagctt gctggaccat cgagccgacg
    12721 gactggaagg tttcgcgggg cgcacgcatg acggtgcggc ttgcgatggt ttcggcatcc
    12781 tcggcggaaa accccgcgtc gatcagttct tgcctgtatg ccttccggtc aaacgtccga
    12841 ttcattcacc ctccttgcgg gattgccccg actcacgccg gggcaatgtg cccttattcc
    12901 tgatttgacc cgcctggtgc cttggtgtcc agataatcca ccttatcggc aatgaagtcg
    12961 gtcccgtaga ccgtctggcc gtccttctcg tacttggtat tccgaatctt gccctgcacg
    13021 aataccagcg accccttgcc caaatacttg ccgtgggcct cggcctgaga gccaaaacac
    13081 ttgatgcgga agaagtcggt gcgctcctgc ttgtcgccgg catcgttgcg ccacatctag
    13141 gtactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat caggcttgat
    13201 ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct ccctgatcga
    13261 ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc caagatcaat
    13321 aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt cgccgtggga
    13381 aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc
    13441 tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat cgttattcag
    13501 taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac aatccgatat
    13561 gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct tttcagggct
    13621 ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca tgagcagatt
    13681 gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct ttccttccag
    13741 caatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat accggctgtc
    13801 cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct tagcaggaga
    13861 cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca attccggtga
    13921 tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt aaagataccc
    13981 caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt
    14041 aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg
    14101 acggagccga ttttgaaacc acaattatgg gtgatgctgc caacttactg atttagtgta
    14161 tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc ctcctgttca
    14221 gctactgacg gggtggtgcg taacggcaaa agcaccgccg gacatcagcg ctatctctgc
    14281 tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca acccggtacg
    14341 caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac agcccgcatt
    14401 atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg cagtcggtaa
    14461 cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac gaacagtggg
    14521 gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac agtctccgga
    14581 agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg cgtcttatga
    14641 gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg ctgtatgaat
    14701 cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga attgagcggc
    14761 ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg tcgttctcaa
    14821 aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa cactatcaat
    14881 aagttggagt cattacccaa ttatgataga atttacaagc tataaggtta ttgtcctggg
    14941 tttcaagcat tagtccatgc aagtttttat gctttgccca ttctatagat atattgataa
    15001 gcgcgctgcc tatgccttgc cccctgaaat ccttacatac ggcgatatct tctatataaa
    15061 agatatatta tcttatcagt attgtcaata tattcaaggc aatctgcctc ctcatcctct
    15121 tcatcctctt cgtcttggta gctttttaaa tatggcgctt catagagtaa ttctgtaaag
    15181 gtccaattct cgttttcata cctcggtata atcttaccta tcacctcaaa tggttcgctg
    15241 ggtttatcgc acccccgaac acgagcacgg cacccgcgac cactatgcca agaatgccca
    15301 aggtaaaaa
//
