LOCUS       sequence-269488-       15095 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15095)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX154
FEATURES             Location/Qualifiers
     source          1..15095
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        258..603
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    625..649
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1289..1313)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1342..1431
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      1465..1717
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(2146..2398)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2866..3891)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(2866..3891)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(3901..4245)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(4355..4372)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4355..4371)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4364..4386)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4511..4530)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4721..4742
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    4989..5013
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(6609..7757)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(8056..8850)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLLLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  9042..9809
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      10432..11143
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(11270..11641)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            11674..11783
                     /note="incP origin of transfer"
     primer_bind     12006..12025
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12259..12276
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(12464..13114)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    13579..13603
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        13702..14047
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    14674..14850
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 ggcttgcttc acaaaccaag gcaagtaata gagattggag tctctaaaaa ggtagttccc
       61 actgaatcaa aggccatgga gtcaaagatt caaatagagg acctaacaga actcgccgta
      121 aagactggcg aacagttcat acagagtctc ttacgactca atgacaagaa gaaaatcttc
      181 gtcaacatgg tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca
      241 gaagaccaaa gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga
      301 ttccattgcc cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc
      361 tacaaatgcc atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt
      421 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc
      481 acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa
      541 tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagaga
      601 acacggggga ctctagagtt atcaacaagt ttgtacaaaa aagcaggctc caccactagt
      661 atggcatcat cggagattgt ggattccggt aggttcgcgg cctccgccgg tggtcagaaa
      721 tcacatttct cacagacatg taatttgttg agccaatact tgaaagagaa gaaaggttca
      781 tttggagatc tcagccttgg tattcaccgc gccggtacta ctactactat ggatttattc
      841 ccaatgattg agaaatctgg tgagtcaaat cctcagaaac caatgaatct gtttcctcaa
      901 actgaagcaa aatctgaacc ggagaaagca cagatgacga tattctatgg cggtcaagtt
      961 attgtgttta acgattttcc ggcagataaa gctaaggaaa tcatgcttat ggctacttgt
     1021 accaaaggaa acaacaacag tactactcag attcaaaaaa cagctgaatc tgcttcagat
     1081 ttggtacctc agcctattat ttctggagat ttaccaattg cgagacgagc ttcacttact
     1141 aggtttttgg agaaaagaaa agataggcta actgcaaaag caccgtatca attaagcaac
     1201 gcaaataaac aagcagcagt ttctgaaaac aaggcgtggc ttggattggg tgctcaattt
     1261 ccagtgaaag ctgagcaatt ccccggggac ccagctttct tgtacaaagt ggttgataac
     1321 agcgggttaa ttaacatctt ttacccatac gatgttcctg actatgcggg ctatccctat
     1381 gacgtcccgg actatgcagg atcctatcca tatgacgttc cagattacgc tgctcagtgc
     1441 agcgcttaga gctcgaattt ccccgatcgt tcaaacattt ggcaataaag tttcttaaga
     1501 ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag
     1561 catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga
     1621 gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat
     1681 aaattatcgc gcgcggtgtc atctatgtta ctagatcggg aattagcttc atcaacgcaa
     1741 gacatgcgca cgaccgtctg acaggagagg aatttccgac gagcacagaa aggacttgct
     1801 cttggacgta ggcctatttc tcaggcacat gtatcaagtg ttcggacgtg ggttttcgat
     1861 ggtgtatcag ccgccgccaa ctgggagatg aggaggcttt cttggggggc agtcagcagt
     1921 tcatttcaca agacagagga acttgtaagg agatgcactg atttatcttg gcgcaaacca
     1981 gcaggacgaa ttagtgggaa tagcccgcga atatctaagt tatgcctgtc ggcatgagca
     2041 gaaacttcca attcgaaaca gtttggagag gttgtttttg ggcatacctt ttgttagtca
     2101 gcctctcgat tgctcatcgt cattacacag taccgaagtt tgatcgatct agtaacatag
     2161 atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt tctatcgcgt
     2221 attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat aacgtcatgc
     2281 attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg ataatcatcg
     2341 caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt gaacgatctg
     2401 cttcgacgca ctccttcttt actccaccat ctcgtcctta ttgaaaacgt gggtagcacc
     2461 aaaacgaatc aagtcgctgg aactgaagtt accaatcacg ctggatgatt tgccagttgg
     2521 attaatcttg cctttccccg catgaataat attgatgaat gcatgcgtga ggggtatttc
     2581 gattttggca atagctgcaa ttgccgcgac atcctccaac gagcataatt cttcagaaaa
     2641 atagcgatgt tccatgttgt cagggcatgc atgatgcacg ttatgaggtg acggtgctag
     2701 gcagtattcc ctcaaagttt catagtcagt atcatattca tcattgcatt cctgcaagag
     2761 agaattgaga cgcaatccac acgctgcggc aaccttccgg cgttcgtggt ctatttgctc
     2821 ttggacgttg caaacgtaag tgttggatcc cggtcggcat ctactctatt cctttgccct
     2881 cggacgagtg ctggggcgtc ggtttccact atcggcgagt acttctacac agccatcggt
     2941 ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg ctccggatcg
     3001 gacgattgcg tcgcatcgac cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa
     3061 gctctgatag agttggtcaa gaccaatgcg gagcatatac gcccggagcc gcggcgatcc
     3121 tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac aagccaacca
     3181 cggcctccag aagaagatgt tggcgacctc gtattgggaa tccccgaaca tcgcctcgct
     3241 ccagtcaatg accgctgtta tgcggccatt gtccgtcagg acattgttgg agccgaaatc
     3301 cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc caaagcatca gctcatcgag
     3361 agcctgcgcg acggacgcac tgacggtgtc gtccatcaca gtttgccagt gatacacatg
     3421 gggatcagca atcgcgcata tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg
     3481 tccgaatggg ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg catccatggc
     3541 ctccgcgacc ggctgcagaa cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac
     3601 accctgtgca cggcgggaga tgcaataggt caggctctcg ctgaattccc caatgtcaag
     3661 cacttccgga atcgggagcg cggccgatgc aaagtgccga taaacataac gatctttgta
     3721 gaaaccatcg gcgcagctat ttacccgcag gacatatcca cgccctccta catcgaagct
     3781 gaaagcacga gattcttcgc cctccgagag ctgcatcagg tcggagacgc tgtcgaactt
     3841 ttcgatcaga aacttctcga cagacgtcgc ggtgagttca ggctttttca tatcggggtc
     3901 gtcctctcca aatgaaatga acttccttat atagaggaag ggtcttgcga aggatagtgg
     3961 gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac
     4021 gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga
     4081 ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg
     4141 taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg
     4201 aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct
     4261 tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgttg
     4321 acggatctct aggacgcgtc ctagaagcta attcactggc cgtcgtttta caacgtcgtg
     4381 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     4441 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     4501 atggcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     4561 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     4621 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     4681 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4741 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
     4801 tcggaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc
     4861 tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga
     4921 aaagaaaaac caccccagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc
     4981 gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg
     5041 cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc
     5101 gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg
     5161 gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt
     5221 aacgatgaca gagcgttgct gcctgtgatc aaatatcatc tccctcgcag agatccgaat
     5281 tatcagcctt cttattcatt tctcgcttaa ccgtgacagg ctgtcgatct tgagaactat
     5341 gccgacataa taggaaatcg ctggataaag ccgctgagga agctgagtgg cgctatttct
     5401 ttagaagtga acgttgacga tatcaactcc cctatccatt gctcaccgaa tggtacaggt
     5461 cggggacccg aagttccgac tgtcggcctg atgcatcccc ggctgatcga ccccagatct
     5521 ggggctgaga aagcccagta aggaaacaac tgtaggttcg agtcgcgaga tcccccggaa
     5581 ccaaaggaag taggttaaac ccgctccgat caggccgagc cacgccaggc cgagaacatt
     5641 ggttcctgta ggcatcggga ttggcggatc aaacactaaa gctactggaa cgagcagaag
     5701 tcctccggcc gccagttgcc aggcggtaaa ggtgagcaga ggcacgggag gttgccactt
     5761 gcgggtcagc acggttccga acgccatgga aaccgccccc gccaggcccg ctgcgacgcc
     5821 gacaggatct agcgctgcgt ttggtgtcaa caccaacagc gccacgcccg cagttccgca
     5881 aatagccccc aggaccgcca tcaatcgtat cgggctacct agcagagcgg cagagatgaa
     5941 cacgaccatc agcggctgca cagcgcctac cgtcgccgcg accccgcccg gcaggcggta
     6001 gaccgaaata aacaacaagc tccagaatag cgaaatatta agtgcgccga ggatgaagat
     6061 gcgcatccac cagattcccg ttggaatctg tcggacgatc atcacgagca ataaacccgc
     6121 cggcaacgcc cgcagcagca taccggcgac ccctcggcct cgctgttcgg gctccacgaa
     6181 aacgccggac agatgcgcct tgtgagcgtc cttggggccg tcctcctgtt tgaagaccga
     6241 cagcccaatg atctcgccgt cgatgtaggc gccgaatgcc acggcatctc gcaaccgttc
     6301 agcgaacgcc tccatgggct ttttctcctc gtgctcgtaa acggacccga acatctctgg
     6361 agctttcttc agggccgaca atcggatctc gcggaaatcc tgcacgtcgg ccgctccaag
     6421 ccgtcgaatc tgagccttaa tcacaattgt caattttaat cctctgttta tcggcagttc
     6481 gtagagcgcg ccgtgcgtcc cgagcgatac tgagcgaagc aagtgcgtcg agcagtgccc
     6541 gcttgttcct gaaatgccag taaagcgctg gctgctgaac ccccagccgg aactgacccc
     6601 acaaggccct agcgtttgca atgcaccagg tcatcattga cccaggcgtg ttccaccagg
     6661 ccgctgcctc gcaactcttc gcaggcttcg ccgacctgct cgcgccactt cttcacgcgg
     6721 gtggaatccg atccgcacat gaggcggaag gtttccagct tgagcgggta cggctcccgg
     6781 tgcgagctga aatagtcgaa catccgtcgg gccgtcggcg acagcttgcg gtacttctcc
     6841 catatgaatt tcgtgtagtg gtcgccagca aacagcacga cgatttcctc gtcgatcagg
     6901 acctggcaac gggacgtttt cttgccacgg tccaggacgc ggaagcggtg cagcagcgac
     6961 accgattcca ggtgcccaac gcggtcggac gtgaagccca tcgccgtcgc ctgtaggcgc
     7021 gacaggcatt cctcggcctt cgtgtaatac cggccattga tcgaccagcc caggtcctgg
     7081 caaagctcgt agaacgtgaa ggtgatcggc tcgccgatag gggtgcgctt cgcgtactcc
     7141 aacacctgct gccacaccag ttcgtcatcg tcggcccgca gctcgacgcc ggtgtaggtg
     7201 atcttcacgt ccttgttgac gtggaaaatg accttgtttt gcagcgcctc gcgcgggatt
     7261 ttcttgttgc gcgtggtgaa cagggcagag cgggccgtgt cgtttggcat cgctcgcatc
     7321 gtgtccggcc acggcgcaat atcgaacaag gaaagctgca tttccttgat ctgctgcttc
     7381 gtgtgtttca gcaacgcggc ctgcttggcc tcgctgacct gttttgccag gtcctcgccg
     7441 gcggtttttc gcttcttggt cgtcatagtt cctcgcgtgt cgatggtcat cgacttcgcc
     7501 aaacctgccg cctcctgttc gagacgacgc gaacgctcca cggcggccga tggcgcgggc
     7561 agggcagggg gagccagttg cacgctgtcg cgctcgatct tggccgtagc ttgctggacc
     7621 atcgagccga cggactggaa ggtttcgcgg ggcgcacgca tgacggtgcg gcttgcgatg
     7681 gtttcggcat cctcggcgga aaaccccgcg tcgatcagtt cttgcctgta tgccttccgg
     7741 tcaaacgtcc gattcattca ccctccttgc gggattgccc cgactcacgc cggggcaatg
     7801 tgcccttatt cctgatttga cccgcctggt gccttggtgt ccagataatc caccttatcg
     7861 gcaatgaagt cggtcccgta gaccgtctgg ccgtccttct cgtacttggt attccgaatc
     7921 ttgccctgca cgaataccag cgaccccttg cccaaatact tgccgtgggc ctcggcctga
     7981 gagccaaaac acttgatgcg gaagaagtcg gtgcgctcct gcttgtcgcc ggcatcgttg
     8041 cgccacatct aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca
     8101 atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc
     8161 ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct
     8221 cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag
     8281 gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag
     8341 ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag
     8401 atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg
     8461 acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat
     8521 cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact
     8581 catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag
     8641 ctttccttcc agcaatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt
     8701 ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac
     8761 cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt
     8821 caattccggt gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat
     8881 ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact gttccttgca
     8941 ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat
     9001 aacatagtat cgacggagcc gattttgaaa ccacaattat gggtgatgct gccaacttac
     9061 tgatttagtg tatgatggtg tttttgaggt gctccagtgg cttctgtttc tatcagctgt
     9121 ccctcctgtt cagctactga cggggtggtg cgtaacggca aaagcaccgc cggacatcag
     9181 cgctatctct gctctcactg ccgtaaaaca tggcaactgc agttcactta caccgcttct
     9241 caacccggta cgcaccagaa aatcattgat atggccatga atggcgttgg atgccgggca
     9301 acagcccgca ttatgggcgt tggcctcaac acgattttac gtcacttaaa aaactcaggc
     9361 cgcagtcggt aacctcgcgc atacagccgg gcagtgacgt catcgtctgc gcggaaatgg
     9421 acgaacagtg gggctatgtc ggggctaaat cgcgccagcg ctggctgttt tacgcgtatg
     9481 acagtctccg gaagacggtt gttgcgcacg tattcggtga acgcactatg gcgacgctgg
     9541 ggcgtcttat gagcctgctg tcaccctttg acgtggtgat atggatgacg gatggctggc
     9601 cgctgtatga atcccgcctg aagggaaagc tgcacgtaat cagcaagcga tatacgcagc
     9661 gaattgagcg gcataacctg aatctgaggc agcacctggc acggctggga cggaagtcgc
     9721 tgtcgttctc aaaatcggtg gagctgcatg acaaagtcat cgggcattat ctgaacataa
     9781 aacactatca ataagttgga gtcattaccc aattatgata gaatttacaa gctataaggt
     9841 tattgtcctg ggtttcaagc attagtccat gcaagttttt atgctttgcc cattctatag
     9901 atatattgat aagcgcgctg cctatgcctt gccccctgaa atccttacat acggcgatat
     9961 cttctatata aaagatatat tatcttatca gtattgtcaa tatattcaag gcaatctgcc
    10021 tcctcatcct cttcatcctc ttcgtcttgg tagcttttta aatatggcgc ttcatagagt
    10081 aattctgtaa aggtccaatt ctcgttttca tacctcggta taatcttacc tatcacctca
    10141 aatggttcgc tgggtttatc gcacccccga acacgagcac ggcacccgcg accactatgc
    10201 caagaatgcc caaggtaaaa attgccggcc ccgccatgaa gtccgtgaat gccccgacgg
    10261 ccgaagtgaa gggcaggccg ccacccaggc cgccgccctc actgcccggc acctggtcgc
    10321 tgaatgtcga tgccagcacc tgcggcacgt caatgcttcc gggcgtcgcg ctcgggctga
    10381 tcgcccatcc cgttactgcc ccgatcccgg caatggcaag gactgccagc gctgccattt
    10441 ttggggtgag gccgttcgcg gccgaggggc gcagcccctg gggggatggg aggcccgcgt
    10501 tagcgggccg ggagggttcg agaagggggg gcacccccct tcggcgtgcg cggtcacgcg
    10561 cacagggcgc agccctggtt aaaaacaagg tttataaata ttggtttaaa agcaggttaa
    10621 aagacaggtt agcggtggcc gaaaaacggg cggaaaccct tgcaaatgct ggattttctg
    10681 cctgtggaca gcccctcaaa tgtcaatagg tgcgcccctc atctgtcagc actctgcccc
    10741 tcaagtgtca aggatcgcgc ccctcatctg tcagtagtcg cgcccctcaa gtgtcaatac
    10801 cgcagggcac ttatccccag gcttgtccac atcatctgtg ggaaactcgc gtaaaatcag
    10861 gcgttttcgc cgatttgcga ggctggccag ctccacgtcg ccggccgaaa tcgagcctgc
    10921 ccctcatctg tcaacgccgc gccgggtgag tcggcccctc aagtgtcaac gtccgcccct
    10981 catctgtcag tgagggccaa gttttccgcg aggtatccac aacgccggcg gccgcggtgt
    11041 ctcgcacacg gcttcgacgg cgtttctggc gcgtttgcag ggccatagac ggccgccagc
    11101 ccagcggcga gggcaaccag cccggtgagc gtcggaaagg cgctcggtct tgccttgctc
    11161 gtcggtgatg tacttcacca gctccgcgaa gtcgctcttc ttgatggagc gcatggggac
    11221 gtgcttggca atcacgcgca ccccccggcc gttttagcgg ctaaaaaagt catggctctg
    11281 ccctcgggcg gaccacgccc atcatgacct tgccaagctc gtcctgcttc tcttcgatct
    11341 tcgccagcag ggcgaggatc gtggcatcac cgaaccgcgc cgtgcgcggg tcgtcggtga
    11401 gccagagttt cagcaggccg cccaggcggc ccaggtcgcc attgatgcgg gccagctcgc
    11461 ggacgtgctc atagtccacg acgcccgtga ttttgtagcc ctggccgacg gccagcaggt
    11521 aggccgacag gctcatgccg gccgccgccg ccttttcctc aatcgctctt cgttcgtctg
    11581 gaaggcagta caccttgata ggtgggctgc ccttcctggt tggcttggtt tcatcagcca
    11641 tccgcttgcc ctcatctgtt acgccggcgg tagccggcca gcctcgcaga gcaggattcc
    11701 cgttgagcac cgccaggtgc gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg
    11761 ggcctacttc acctatcctg cccggctgac gccgttggat acaccaagga aagtctacac
    11821 gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga tggatatacc gaaaaaatcg
    11881 ctataatgac cccgaagcag ggttatgcag cggaaaagcg ccacgcttcc cgaagggaga
    11941 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
    12001 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
    12061 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
    12121 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
    12181 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
    12241 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg ccagaaggcc
    12301 gccagagagg ccgagcgcgg ccgtgaggct tggacgctag ggcagggcat gaaaaagccc
    12361 gtagcgggct gctacgggcg tctgacgcgg tggaaagggg gaggggatgt tgtctacatg
    12421 gctctgctgt agtgagtggg ttgcgctccg gcagcggtcc tgatcaatcg tcaccctttc
    12481 tcggtccttc aacgttcctg acaacgagcc tccttttcgc caatccatcg acaatcaccg
    12541 cgagtccctg ctcgaacgct gcgtccggac cggcttcgtc gaaggcgtct atcgcggccc
    12601 gcaacagcgg cgagagcgga gcctgttcaa cggtgccgcc gcgctcgccg gcatcgctgt
    12661 cgccggcctg ctcctcaagc acggccccaa cagtgaagta gctgattgtc atcagcgcat
    12721 tgacggcgtc cccggccgaa aaacccgcct cgcagaggaa gcgaagctgc gcgtcggccg
    12781 tttccatctg cggtgcgccc ggtcgcgtgc cggcatggat gcgcgcgcca tcgcggtagg
    12841 cgagcagcgc ctgcctgaag ctgcgggcat tcccgatcag aaatgagcgc cagtcgtcgt
    12901 cggctctcgg caccgaatgc gtatgattct ccgccagcat ggcttcggcc agtgcgtcga
    12961 gcagcgcccg cttgttcctg aagtgccagt aaagcgccgg ctgctgaacc cccaaccgtt
    13021 ccgccagttt gcgtgtcgtc agaccgtcta cgccgacctc gttcaacagg tccagggcgg
    13081 cacggatcac tgtattcggc tgcaactttg tcatgcttga cactttatca ctgataaaca
    13141 taatatgtcc accaacttat cagtgataaa gaatccgcgc gttcaatcgg accagcggag
    13201 gctggtccgg aggccagacg tgaaacccaa catacccctg atcgtaattc tgagcactgt
    13261 cgcgctcgac gctgtcggca tcggcctgat tatgccggtg ctgccgggcc tcctgcgcga
    13321 tctggttcac tcgaacgacg tcaccgccca ctatggcatt ctgctggcgc tgtatgcgtt
    13381 ggtgcaattt gcctgcgcac ctgtgctggg cgcgctgtcg gatcgtttcg ggcggcggcc
    13441 aatcttgctc gtctcgctgg ccggcgccag atctggggaa ccctgtggtt ggcatgcaca
    13501 tacaaatgga cgaacggata aaccttttca cgccctttta aatatccgat tattctaata
    13561 aacgctcttt tctcttaggt ttacccgcca atatatcctg tcaaacactg atagtttaaa
    13621 cgttgcgatt aagaattcga gctcggtacc cccctactcc aaaaatgtca aagatacagt
    13681 ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatttcgg gaaacctcct
    13741 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaaggtgg
    13801 ctcctacaaa tgccatcatt gcgataaagg aaaggctatc attcaagatg cctctgccga
    13861 cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc
    13921 aaccacgtct tcaaagcaag tggattgatg tgacatctcc actgacgtaa gggatgacgc
    13981 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
    14041 gaggacagcc caagcttcga ctctagagga tccccttaaa tcgatatgga acgagctata
    14101 caaggaaacg acgctaggga acaagctaac agtgaacgtt gggatggagg atcaggaggt
    14161 accacttctc ccttcaaact tcctgacgaa agtccgagtt ggactgagtg gcggctacat
    14221 aacgatgaga cgaattcgaa tcaagataat ccccttggtt tcaaggaaag ctggggtttc
    14281 gggaaagttg tatttaagag atatctcaga tacgacagga cggaagcttc actgcacaga
    14341 gtccttggat cttggacggg agattcggtt aactatgcag catctcgatt tttcggtttc
    14401 gaccagatcg gatgtaccta tagtattcgg tttcgaggag ttagtatcac cgtttctgga
    14461 gggtctcgaa ctcttcagca tctctgtgag atggcaattc ggtctaagca agaactgcta
    14521 cagcttgccc caatcgaagt ggaaagtaat gtatcaagag gatgccctga aggtactcaa
    14581 accttcgaaa aagaaagcga gtaagtcgag ggcgagctcg aattcggtac gctgaaatca
    14641 ccagtctctc tctacaaatc tatctctctc tattttctcc ataaataatg tgtgagtagt
    14701 ttcccgataa gggaaattag ggttcttata gggtttcgct catgtgttga gcatataaga
    14761 aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta
    14821 aaaccaaaat ccagtactaa aatccagatc tccctgcagg tccccagatt agccttttca
    14881 atttcagaaa gaatgctaac ccacagatgg ttagagaggc ttacgcagca ggtctcatca
    14941 agacgatcta cccgagcaat aatctccagg aaatcaaata ccttcccaag aaggttaaag
    15001 atgcagtcaa aagattcagg actaactgca tcaagaacac agagaaagat atatttctca
    15061 agatcagaag tactattcca gtatggacga ttcaa
//
