LOCUS       sequence-269489-        7502 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression human MSH2 G674 mutant protein using baculovirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7502)
  AUTHORS   Geng H, Du C, Chen S, Salerno V, Manfredi C, Hsieh P
  TITLE     In vitro studies of DNA mismatch repair proteins.
  JOURNAL   Anal Biochem. 2011 Jun 15;413(2):179-84. doi: 
            10.1016/j.ab.2011.02.017. Epub 2011 Feb 15.
  PUBMED    21329650
REFERENCE   2  (bases 1 to 7502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ab.2011.02.017"; journalName: "Anal Biochem"; date: 
            "2011-06-15- 15"; volume: "413"; issue: "2"; pages: "179-84"
COMMENT     Sequence Label: pFB1_hMSH2_G674A
FEATURES             Location/Qualifiers
     source          1..7502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    3089..3223
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3126..3145)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3180..3199
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3252..3417
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3601..4055
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3688..3707)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3897..3918
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4081..4185
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4186..4254,4255..5046)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4404..4423)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5217..5805
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5706..5725
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5959..5976
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  6110..6334
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(6401..6934)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(7123..7128,7129..7145,7146..7151))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atccacatgg cggtgcagcc gaaggagacg ctgcagttgg agagcgcggc
      181 cgaggtcggc ttcgtgcgct tctttcaggg catgccggag aagccgacca ccacagtgcg
      241 ccttttcgac cggggcgact tctatacggc gcacggcgag gacgcgctgc tggccgcccg
      301 ggaggtgttc aagacccagg gggtgatcaa gtacatgggg ccggcaggag caaagaatct
      361 gcagagtgtt gtgcttagta aaatgaattt tgaatctttt gtaaaagatc ttcttctggt
      421 tcgtcagtat agagttgaag tttataagaa tagagctgga aataaggcat ccaaggagaa
      481 tgattggtat ttggcatata aggcttctcc tggcaatctc tctcagtttg aagacattct
      541 ctttggtaac aatgatatgt cagcttccat tggtgttgtg ggtgttaaaa tgtccgcagt
      601 tgatggccag agacaggttg gagttgggta tgtggattcc atacagagga aactaggact
      661 gtgtgaattc cctgataatg atcagttctc caatcttgag gctctcctca tccagattgg
      721 accaaaggaa tgtgttttac ccggaggaga gactgctgga gacatgggga aactgagaca
      781 gataattcaa agaggaggaa ttctgatcac agaaagaaaa aaagctgact tttccacaaa
      841 agacatttat caggacctca accggttgtt gaaaggcaaa aagggagagc agatgaatag
      901 tgctgtattg ccagaaatgg agaatcaggt tgcagtttca tcactgtctg cggtaatcaa
      961 gtttttagaa ctcttatcag atgattccaa ctttggacag tttgaactga ctacttttga
     1021 cttcagccag tatatgaaat tggatattgc agcagtcaga gcccttaacc tttttcaggg
     1081 ttctgttgaa gataccactg gctctcagtc tctggctgcc ttgctgaata agtgtaaaac
     1141 ccctcaagga caaagacttg ttaaccagtg gattaagcag cctctcatgg ataagaacag
     1201 aatagaggag agattgaatt tagtggaagc ttttgtagaa gatgcagaat tgaggcagac
     1261 tttacaagaa gatttacttc gtcgattccc agatcttaac cgacttgcca agaagtttca
     1321 aagacaagca gcaaacttac aagattgtta ccgactctat cagggtataa atcaactacc
     1381 taatgttata caggctctgg aaaaacatga aggaaaacac cagaaattat tgttggcagt
     1441 ttttgtgact cctcttactg atcttcgttc tgacttctcc aagtttcagg aaatgataga
     1501 aacaacttta gatatggatc aggtggaaaa ccatgaattc cttgtaaaac cttcatttga
     1561 tcctaatctc agtgaattaa gagaaataat gaatgacttg gaaaagaaga tgcagtcaac
     1621 attaataagt gcagccagag atcttggctt ggaccctggc aaacagatta aactggattc
     1681 cagtgcacag tttggatatt actttcgtgt aacctgtaag gaagaaaaag tccttcgtaa
     1741 caataaaaac tttagtactg tagatatcca gaagaatggt gttaaattta ccaacagcaa
     1801 attgacttct ttaaatgaag agtataccaa aaataaaaca gaatatgaag aagcccagga
     1861 tgccattgtt aaagaaattg tcaatatttc ttcaggctat gtagaaccaa tgcagacact
     1921 caatgatgtg ttagctcagc tagatgctgt tgtcagcttt gctcacgtgt caaatggagc
     1981 acctgttcca tatgtacgac cagccatttt ggagaaagga caaggaagaa ttatattaaa
     2041 agcatccagg catgcttgtg ttgaagttca agatgaaatt gcatttattc ctaatgacgt
     2101 atactttgaa aaagataaac agatgttcca catcattact ggccccaata tgggagctaa
     2161 atcaacatat attcgacaaa ctggggtgat agtactcatg gcccaaattg ggtgttttgt
     2221 gccatgtgag tcagcagaag tgtccattgt ggactgcatc ttagcccgag taggggctgg
     2281 tgacagtcaa ttgaaaggag tctccacgtt catggctgaa atgttggaaa ctgcttctat
     2341 cctcaggtct gcaaccaaag attcattaat aatcatagat gaattgggaa gaggaacttc
     2401 tacctacgat ggatttgggt tagcatgggc tatatcagaa tacattgcaa caaagattgg
     2461 tgctttttgc atgtttgcaa cccattttca tgaacttact gccttggcca atcagatacc
     2521 aactgttaat aatctacatg tcacagcact caccactgaa gagaccttaa ctatgcttta
     2581 tcaggtgaag aaaggtgtct gtgatcaaag ttttgggatt catgttgcag agcttgctaa
     2641 tttccctaag catgtaatag agtgtgctaa acagaaagcc ctggaacttg aggagtttca
     2701 gtatattgga gaatcgcaag gatatgatat catggaacca gcagcaaaga agtgctatct
     2761 ggaaagagag caaggtgaaa aaattattca ggagttcctg tccaaggtga aacaaatgcc
     2821 ctttactgaa atgtcagaag aaaacatcac aataaagtta aaacagctaa aagctgaagt
     2881 aatagcaaag aataatagct ttgtaaatga aatcatttca cgaataaaag ttactacgtg
     2941 actcgaggca tgcggtacca agcttgtcga gaagtactag aggatcataa tcagccatac
     3001 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     3061 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     3121 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     3181 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgatcac tgcttgagcc
     3241 taggagatcc gaaccagata agtgaaatct agttccaaac tattttgtca tttttaattt
     3301 tcgtattagc ttacgacgct acacccagtt cccatctatt ttgtcactct tccctaaata
     3361 atccttaaaa actccatttc cacccctccc agttcccaac tattttgtcc gcccacagcg
     3421 gggcattttt cttcctgtta tgtttttaat caaacatcct gccaactcca tgtgacaaac
     3481 cgtcatcttc ggctactttt tctctgtcac agaatgaaaa tttttctgtc atctcttcgt
     3541 tattaatgtt tgtaattgac tgaatatcaa cgcttatttg cagcctgaat ggcgaatggg
     3601 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     3661 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     3721 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     3781 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     3841 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc ttaatagtgg
     3901 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     3961 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4021 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     4081 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     4141 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     4201 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     4261 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     4321 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     4381 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     4441 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     4501 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     4561 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     4621 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     4681 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     4741 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     4801 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     4861 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     4921 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     4981 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     5041 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     5101 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     5161 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5221 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5281 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5341 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     5401 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     5461 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5521 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     5581 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     5641 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     5701 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     5761 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     5821 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     5881 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     5941 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     6001 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tagaccagcc gcgtaacctg
     6061 gcaaaatcgg ttacggttga gtaataaatg gatgccctgc gtaagcgggt gtgggcggac
     6121 aataaagtct taaactgaac aaaatagatc taaactatga caataaagtc ttaaactaga
     6181 cagaatagtt gtaaactgaa atcagtccag ttatgctgtg aaaaagcata ctggactttt
     6241 gttatggcta aagcaaactc ttcattttct gaagtgcaaa ttgcccgtcg tattaaagag
     6301 gggcgtggcc aagggcatgg taaagactat attcgcggcg ttgtgacaat ttaccgaaca
     6361 actccgcggc cgggaagccg atctcggctt gaacgaattg ttaggtggcg gtacttgggt
     6421 cgatatcaaa gtgcatcact tcttcccgta tgcccaactt tgtatagaga gccactgcgg
     6481 gatcgtcacc gtaatctgct tgcacgtaga tcacataagc accaagcgcg ttggcctcat
     6541 gcttgaggag attgatgagc gcggtggcaa tgccctgcct ccggtgctcg ccggagactg
     6601 cgagatcata gatatagatc tcactacgcg gctgctcaaa cttgggcaga acgtaagccg
     6661 cgagagcgcc aacaaccgct tcttggtcga aggcagcaag cgcgatgaat gtcttactac
     6721 ggagcaagtt cccgaggtaa tcggagtccg gctgatgttg ggagtaggtg gctacgtctc
     6781 cgaactcacg accgaaaaga tcaagagcag cccgcatgga tttgacttgg tcagggccga
     6841 gcctacatgt gcgaatgatg cccatacttg agccacctaa ctttgtttta gggcgactgc
     6901 cctgctgcgt aacatcgttg ctgctgcgta acatcgttgc tgctccataa catcaaacat
     6961 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa
     7021 cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag
     7081 gttctggacc agttgcgtga gcgcatacgc tacttgcatt acagtttacg aaccgaacag
     7141 gcttatgtca actgggttcg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc
     7201 ttgggcagca gcgaagtcga ggcatttctg tcctggctgg cgaacgagcg caaggtttcg
     7261 gtctccacgc atcgtcaggc attggcggcc ttgctgttct tctacggcaa ggtgctgtgc
     7321 acggatctgc cctggcttca ggagatcgga agacctcggc cgtcgcggcg cttgccggtg
     7381 gtgctgaccc cggatgaagt ggttcgcatc ctcggttttc tggaaggcga gcatcgtttg
     7441 ttcgcccagg actctagcta tagttctagt ggttggctac gtatactccg gaatattaat
     7501 ag
//
