LOCUS       sequence-269492-        5728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5728)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 5728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dronpa-MAPTau-N-10
FEATURES             Location/Qualifiers
     source          1..5728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        158..461
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        462..665
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     616..636
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      716..725
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1802..1811
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1808..2485
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
     polyA_signal    2613..2734
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2650..2669)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2704..2723
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2741..3196)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2878..2899)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3090..3109
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3223..3327
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3329..3686
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3329..3520
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3352..3372)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3537..3672
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3721..4515
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3721..4515
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3775..3794)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4385..4404
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4703..4722)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4747..4794
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5123..5711
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5612..5631
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
       61 ccctgattct gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta
      121 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      241 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      301 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      361 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      421 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      481 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      541 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      601 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      661 gagctggttt agtgaaccgt cagatccgct agcgctaccg gactcagatc tcgaggccac
      721 catggctgag ccccgccagg agttcgaagt gatggaagat cacgctggga cgtacgggtt
      781 gggggacagg aaagatcagg ggggctacac catgcaccaa gaccaagagg gtgacacgga
      841 cgctggcctg aaagctgaag aagcaggcat tggagacacc cccagcctgg aagacgaagc
      901 tgctggtcac gtgacccaag ctcgcatggt cagtaaaagc aaagacggga ctggaagcga
      961 tgacaaaaaa gccaaggggg ctgatggtaa aacgaagatc gccacaccgc ggggagcagc
     1021 ccctccaggc cagaagggcc aggccaacgc caccaggatt ccagcaaaaa ccccgcccgc
     1081 tccaaagaca ccacccagct ctggtgaacc tccaaaatca ggggatcgca gcggctacag
     1141 cagccccggc tccccaggca ctcccggcag ccgctcccgc accccgtccc ttccaacccc
     1201 acccacccgg gagcccaaga aggtggcagt ggtccgtact ccacccaagt cgccgtcttc
     1261 cgccaagagc cgcctgcaga cagcccccgt gcccatgcca gacctgaaga atgtcaagtc
     1321 caagatcggc tccactgaga acctgaagca ccagccggga ggcgggaagg tgcaaatagt
     1381 ctacaaacca gttgacctga gcaaggtgac ctccaagtgt ggctcattag gcaacatcca
     1441 tcataaacca ggaggtggcc aggtggaagt aaaatctgag aagcttgact tcaaggacag
     1501 agtccagtcg aagattgggt ccctggacaa tatcacccac gtccctggcg gaggaaataa
     1561 aaagattgaa acccacaagc tgaccttccg cgagaacgcc aaagccaaga cagaccacgg
     1621 ggcggagatc gtgtacaagt cgccagtggt gtctggggac acgtctccac ggcatctcag
     1681 caatgtctcc tccaccggca gcatcgacat ggtagactcg ccccagctcg ccacgctagc
     1741 tgacgaggtg tctgcctccc tggccaagca gggtttgagc tccggagggg atccaccggt
     1801 cgccaccatg gtgagtgtga ttaaaccaga catgaagatc aagctgcgta tggaaggcgc
     1861 tgtaaatgga cacccgttcg cgattgaagg agttggcctt gggaagcctt tcgagggaaa
     1921 acagagtatg gaccttaaag tcaaagaagg cggacctctg cctttcgcct atgacatctt
     1981 gacaactgtg ttctgttacg gcaacagggt attcgccaaa tacccagaaa atatagtaga
     2041 ctatttcaag cagtcgtttc ctgagggcta ctcttgggaa cgaagcatga attacgaaga
     2101 cgggggcatt tgtaacgcga caaacgacat aaccctggat ggtgactgtt atatctatga
     2161 aattcgattt gatggtgtga actttcctgc caatggtcca gttatgcaga agaggactgt
     2221 gaaatgggag ccatccactg agaaattgta tgtgcgtgat ggagtgctga agggtgatgt
     2281 taacatggct ctgtcgcttg aaggaggtgg ccattaccga tgtgacttca aaactactta
     2341 taaagctaag aaggttgtcc agttgccaga ctatcacttt gtggaccacc acattgagat
     2401 taaaagccac gacaaagatt acagtaatgt taatctgcat gagcacgccg aagcgcattc
     2461 tgagctgccg aggcaggcca agtaatgata ggcggccgcg actctagatc ataatcagcc
     2521 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     2581 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     2641 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     2701 gttgtggttt gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt
     2761 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     2821 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     2881 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     2941 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     3001 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     3061 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     3121 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     3181 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     3241 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     3301 cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt
     3361 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     3421 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     3481 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     3541 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     3601 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     3661 tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc
     3721 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     3781 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     3841 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     3901 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     3961 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     4021 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     4081 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     4141 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     4201 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
     4261 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     4321 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     4381 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     4441 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     4501 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     4561 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     4621 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     4681 cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
     4741 ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac
     4801 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc
     4861 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag
     4921 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac
     4981 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5041 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5101 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5161 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5221 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     5281 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5341 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     5401 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     5461 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     5521 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     5581 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5641 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5701 gcctatggaa aaacgccagc aacgcggc
//
