LOCUS       sequence-269512-       11591 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Neuronal expression of N-terminal intein-split prime editor v2 (PE2)
            with EGFP-KASH marker and U6-mDNMT1 cassette. This plasmid is used 
            by PE2, PE3, and PE3b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11591)
  AUTHORS   Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, 
            Chen PJ, Wilson C, Newby GA, Raguram A, Liu DR
  TITLE     Search-and-replace genome editing without double-strand breaks or 
            donor DNA.
  JOURNAL   Nature. 2019 Oct 21. pii: 10.1038/s41586-019-1711-4. doi: 
            10.1038/s41586-019-1711-4.
  PUBMED    31634902
REFERENCE   2  (bases 1 to 11591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2019 Oct 21. pii: 10.1038/s41586-019-1711-4. doi: 
            10.1038/s41586-019-1711-4."
COMMENT     Sequence Label: U6-DNMT1-hSynapsin-PE2-Nterminal-P2A-EGFP-KASH...
FEATURES             Location/Qualifiers
     source          1..11591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..234
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             419..463
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    761..878
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        944..1192
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     944..964
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1115..1134
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        1214..1289
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        1328..1775
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             1836..1856
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1857..3572
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             3921..3941
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3951..4007
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(4008..4010,4011..4721)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4008..4010,4011..4721)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4050..4071)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4311..4330)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4658..4679
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5004..5592
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5057..5077)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5475..5486)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5664..5897
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5923..5940)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5929..6153
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6199..6627
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6286..6305)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6496..6517
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6636..6656)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6641..6970
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6821..6956
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6883..6902
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7018..7065
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7084..7458
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7588..7709
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7625..7644)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7679..7698
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7758..7774)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7758..7774)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7771..7793)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7782..7798
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7806..7812,7813..7830,7831..7836))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7851..7872
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7989..8006)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8160..8748)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8240..8259)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8919..9710,9711..9779))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9542..9561
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9780..9884)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9959..9978)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10150..10529
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10531..10729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10684..10704
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10747..10927
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10974..11099
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
       61 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
      121 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
      181 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
      241 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
      301 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
      361 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
      421 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
      481 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
      541 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
      601 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
      661 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat cggcactgcg
      721 tgcgccaatt ctgcagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
      781 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      841 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      901 gagatccagt ttggttaatt aaggtaccaa ggtcgggcag gaagagggcc tatttcccat
      961 gattccttca tatttgcata tacgatacaa ggctgttaga gagataatta gaattaattt
     1021 gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg
     1081 gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg
     1141 aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccgcgggctg
     1201 gagctgttcg cgcgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
     1261 aacttgaaaa agtggcaccg agtcggtgca agatgcaagc gcgaacagct ccagtttttt
     1321 tgaattcagt gcaagtgggt tttaggacca ggatgaggcg gggtgggggt gcctacctga
     1381 cgaccgaccc cgacccactg gacaagcacc caacccccat tccccaaatt gcgcatcccc
     1441 tatcagagag ggggagggga aacaggatgc ggcgaggcgc gtgcgcactg ccagcttcag
     1501 caccgcggac agtgccttcg cccccgcctg gcggcgcgcg ccaccgccgc ctcagcactg
     1561 aaggcgcgct gacgtcactc gccggtcccc cgcaaactcc ccttcccggc caccttggtc
     1621 gcgtccgcgc cgccgccggc ccagccggac cgcaccacgc gaggcgcgag ataggggggc
     1681 acgggcgcga ccatctgcgc tgcggcgccg gcgactcagc gctgcctcag tctgcggtgg
     1741 gcagcggagg agtcgtgtcg tgcctgagag cgcagtcgag aatctagagc gctgccacca
     1801 tgaaacggac agccgacgga agcgagttcg agtcaccaaa gaagaagcgg aaagtcgaca
     1861 agaagtacag catcggcctg gacatcggca ccaactctgt gggctgggcc gtgatcaccg
     1921 acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca
     1981 tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc
     2041 ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc
     2101 aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag
     2161 agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg
     2221 tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg
     2281 tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca
     2341 agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca
     2401 agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg
     2461 ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg
     2521 aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcgga aacctgattg
     2581 ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc gaggatgcca
     2641 aactgcagct gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg
     2701 gcgaccagta cgccgacctg tttctggccg ccaagaacct gtccgacgcc atcctgctga
     2761 gcgacatcct gagagtgaac accgagatca ccaaggcccc cctgagcgcc tctatgatca
     2821 agagatacga cgagcaccac caggacctga ccctgctgaa agctctcgtg cggcagcagc
     2881 tgcctgagaa gtacaaagag attttcttcg accagagcaa gaacggctac gccggctaca
     2941 ttgacggcgg agccagccag gaagagttct acaagttcat caagcccatc ctggaaaaga
     3001 tggacggcac cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc
     3061 ggaccttcga caacggcagc atcccccacc agatccacct gggagagctg cacgccattc
     3121 tgcggcggca ggaagatttt tacccattcc tgaaggacaa ccgggaaaag atcgagaaga
     3181 tcctgacctt ccgcatcccc tactacgtgg gccctctggc caggggaaac agcagattcg
     3241 cctggatgac cagaaagagc gaggaaacca tcaccccctg gaacttcgag gaagtggtgg
     3301 acaagggcgc ttccgcccag agcttcatcg agcggatgac caacttcgat aagaacctgc
     3361 ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga gtacttcacc gtgtataacg
     3421 agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg
     3481 agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc
     3541 agctgaaaga ggactacttc aagaaaatcg agtgcctgtc ctacgagaca gagatcctga
     3601 cagtggagta tggcctgctg ccaatcggca agatcgtgga gaagaggatc gagtgtaccg
     3661 tgtactctgt ggataacaat ggcaacatct atacacagcc cgtggcacag tggcacgata
     3721 ggggagagca ggaggtgttc gagtattgcc tggaggacgg cagcctgatc agggcaacca
     3781 aggaccacaa gttcatgaca gtggatggcc agatgctgcc catcgacgag attttcgagc
     3841 gggagctgga cctgatgaga gtggataacc tgcctaatag cggaggcagt aaaagaacag
     3901 cagacgggag tgagtttgag cccaagaaaa agagaaaggt gggatccggc gcaacaaact
     3961 tctctctgct gaaacaagcc ggagatgtcg aagagaatcc tggaccggtg agcaagggcg
     4021 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     4081 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     4141 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     4201 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     4261 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     4321 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     4381 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     4441 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     4501 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     4561 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     4621 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     4681 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtccggactc agatctcgag
     4741 aggaggagga ggagacagac agcaggatgc cccacctcga cagccccggc agctcccagc
     4801 cgagacgctc cttcctctca agggtgatca gggcagcgct accgttgcag ctgcttctgc
     4861 tgctgctgct gctcctggcc tgcctgctac ctgcctctga agatgactac agctgcaccc
     4921 aggccaacaa ctttgcccga tccttctacc ccatgctgcg gtacaccaac gggccacctc
     4981 ccacctaaac gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     5041 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     5101 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     5161 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     5221 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     5281 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     5341 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     5401 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     5461 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     5521 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     5581 ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca
     5641 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     5701 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     5761 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     5821 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     5881 gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     5941 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     6001 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     6061 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     6121 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     6181 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     6241 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     6301 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     6361 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     6421 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     6481 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     6541 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     6601 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     6661 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     6721 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     6781 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     6841 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     6901 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     6961 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt
     7021 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa
     7081 accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg
     7141 gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc
     7201 ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg
     7261 gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg
     7321 gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag
     7381 cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg
     7441 gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc cttctatgaa
     7501 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     7561 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     7621 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     7681 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     7741 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     7801 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     7861 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     7921 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     7981 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     8041 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     8101 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     8161 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     8221 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     8281 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     8341 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     8401 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8461 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8521 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8581 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8641 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8701 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8761 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8821 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     8881 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     8941 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     9001 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     9061 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     9121 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     9181 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     9241 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     9301 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     9361 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     9421 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     9481 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     9541 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     9601 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     9661 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     9721 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     9781 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     9841 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     9901 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta
     9961 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
    10021 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
    10081 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
    10141 atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
    10201 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
    10261 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
    10321 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
    10381 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
    10441 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
    10501 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
    10561 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
    10621 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
    10681 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcagc gcgttttgcc
    10741 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
    10801 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
    10861 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
    10921 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
    10981 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
    11041 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
    11101 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
    11161 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
    11221 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
    11281 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
    11341 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
    11401 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
    11461 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
    11521 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
    11581 gcagtgggaa t
//
