LOCUS       sequence-269513-       12746 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Neuronal expression of C-terminal intein-split prime editor v2 
            (PE2). This plasmid is used by PE2, PE3, and PE3b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12746)
  AUTHORS   Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, 
            Chen PJ, Wilson C, Newby GA, Raguram A, Liu DR
  TITLE     Search-and-replace genome editing without double-strand breaks or 
            donor DNA.
  JOURNAL   Nature. 2019 Oct 21. pii: 10.1038/s41586-019-1711-4. doi: 
            10.1038/s41586-019-1711-4.
  PUBMED    31634902
REFERENCE   2  (bases 1 to 12746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2019 Oct 21. pii: 10.1038/s41586-019-1711-4. doi: 
            10.1038/s41586-019-1711-4."
COMMENT     Sequence Label: hSynapsin-PE2-Cterminal-lenti
FEATURES             Location/Qualifiers
     source          1..12746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1606..1723
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1774..2221
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             2282..2302
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2408..4792
                     /codon_start=1
                     /product="C-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(C)
                     /translation="CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILED
                     IVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGK
                     TILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKG
                     ILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQI
                     LKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDN
                     KVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDK
                     AGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK
                     ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMP
                     QVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKV
                     EKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELEN
                     GRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLD
                     EIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKY
                     FDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     CDS             6965..6985
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    7004..7592
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7057..7077)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7475..7486)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7664..7897
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7923..7940)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7929..8153
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8199..8627
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8286..8305)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8496..8517
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8636..8656)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8641..8970
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8821..8956
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8883..8902
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9018..9065
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9084..9458
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9588..9709
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9625..9644)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9679..9698
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9758..9774)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9758..9774)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9771..9793)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9782..9798
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9806..9812,9813..9830,9831..9836))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9851..9872
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9989..10006)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10160..10748)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10240..10259)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10919..11710,11711..11779))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11542..11561
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11780..11884)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11959..11978)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        12150..12529
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12531..12729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12684..12704
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     1561 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     1621 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1681 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1741 cagcagagat ccagtttggt taattaaggt accagtgcaa gtgggtttta ggaccaggat
     1801 gaggcggggt gggggtgcct acctgacgac cgaccccgac ccactggaca agcacccaac
     1861 ccccattccc caaattgcgc atcccctatc agagaggggg aggggaaaca ggatgcggcg
     1921 aggcgcgtgc gcactgccag cttcagcacc gcggacagtg ccttcgcccc cgcctggcgg
     1981 cgcgcgccac cgccgcctca gcactgaagg cgcgctgacg tcactcgccg gtcccccgca
     2041 aactcccctt cccggccacc ttggtcgcgt ccgcgccgcc gccggcccag ccggaccgca
     2101 ccacgcgagg cgcgagatag gggggcacgg gcgcgaccat ctgcgctgcg gcgccggcga
     2161 ctcagcgctg cctcagtctg cggtgggcag cggaggagtc gtgtcgtgcc tgagagcgca
     2221 gtcgagaatc tagagcgctg ccaccatgaa acggacagcc gacggaagcg agttcgagtc
     2281 accaaagaag aagcggaaag tcatcaagat tgctacacgg aaatacctgg gaaagcagaa
     2341 cgtgtacgac atcggcgtgg agcgggatca caacttcgcc ctgaagaatg gctttatcgc
     2401 cagcaattgc ttcgactccg tggaaatctc cggcgtggaa gatcggttca acgcctccct
     2461 gggcacatac cacgatctgc tgaaaattat caaggacaag gacttcctgg acaatgagga
     2521 aaacgaggac attctggaag atatcgtgct gaccctgaca ctgtttgagg acagagagat
     2581 gatcgaggaa cggctgaaaa cctatgccca cctgttcgac gacaaagtga tgaagcagct
     2641 gaagcggcgg agatacaccg gctggggcag gctgagccgg aagctgatca acggcatccg
     2701 ggacaagcag tccggcaaga caatcctgga tttcctgaag tccgacggct tcgccaacag
     2761 aaacttcatg cagctgatcc acgacgacag cctgaccttt aaagaggaca tccagaaagc
     2821 ccaggtgtcc ggccagggcg atagcctgca cgagcacatt gccaatctgg ccggcagccc
     2881 cgccattaag aagggcatcc tgcagacagt gaaggtggtg gacgagctcg tgaaagtgat
     2941 gggccggcac aagcccgaga acatcgtgat cgaaatggcc agagagaacc agaccaccca
     3001 gaagggacag aagaacagcc gcgagagaat gaagcggatc gaagagggca tcaaagagct
     3061 gggcagccag atcctgaaag aacaccccgt ggaaaacacc cagctgcaga acgagaagct
     3121 gtacctgtac tacctgcaga atgggcggga tatgtacgtg gaccaggaac tggacatcaa
     3181 ccggctgtcc gactacgatg tggacgctat cgtgcctcag agctttctga aggacgactc
     3241 catcgacaac aaggtgctga ccagaagcga caagaaccgg ggcaagagcg acaacgtgcc
     3301 ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg cagctgctga acgccaagct
     3361 gattacccag agaaagttcg acaatctgac caaggccgag agaggcggcc tgagcgaact
     3421 ggataaggcc ggcttcatca agagacagct ggtggaaacc cggcagatca caaagcacgt
     3481 ggcacagatc ctggactccc ggatgaacac taagtacgac gagaatgaca agctgatccg
     3541 ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga aggatttcca
     3601 gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct acctgaacgc
     3661 cgtcgtggga accgccctga tcaaaaagta ccctaagctg gaaagcgagt tcgtgtacgg
     3721 cgactacaag gtgtacgacg tgcggaagat gatcgccaag agcgagcagg aaatcggcaa
     3781 ggctaccgcc aagtacttct tctacagcaa catcatgaac tttttcaaga ccgagattac
     3841 cctggccaac ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg aaaccgggga
     3901 gatcgtgtgg gataagggcc gggattttgc caccgtgcgg aaagtgctga gcatgcccca
     3961 agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag agtctatcct
     4021 gcccaagagg aacagcgata agctgatcgc cagaaagaag gactgggacc ctaagaagta
     4081 cggcggcttc gacagcccca ccgtggccta ttctgtgctg gtggtggcca aagtggaaaa
     4141 gggcaagtcc aagaaactga agagtgtgaa agagctgctg gggatcacca tcatggaaag
     4201 aagcagcttc gagaagaatc ccatcgactt tctggaagcc aagggctaca aagaagtgaa
     4261 aaaggacctg atcatcaagc tgcctaagta ctccctgttc gagctggaaa acggccggaa
     4321 gagaatgctg gcctctgccg gcgaactgca gaagggaaac gaactggccc tgccctccaa
     4381 atatgtgaac ttcctgtacc tggccagcca ctatgagaag ctgaagggct cccccgagga
     4441 taatgagcag aaacagctgt ttgtggaaca gcacaagcac tacctggacg agatcatcga
     4501 gcagatcagc gagttctcca agagagtgat cctggccgac gctaatctgg acaaagtgct
     4561 gtccgcctac aacaagcacc gggataagcc catcagagag caggccgaga atatcatcca
     4621 cctgtttacc ctgaccaatc tgggagcccc tgccgccttc aagtactttg acaccaccat
     4681 cgaccggaag aggtacacca gcaccaaaga ggtgctggac gccaccctga tccaccagag
     4741 catcaccggc ctgtacgaga cacggatcga cctgtctcag ctgggaggtg actctggagg
     4801 atctagcgga ggatcctctg gcagcgagac accaggaaca agcgagtcag caacaccaga
     4861 gagcagtggc ggcagcagcg gcggcagcag caccctaaat atagaagatg agtatcggct
     4921 acatgagacc tcaaaagagc cagatgtttc tctagggtcc acatggctgt ctgattttcc
     4981 tcaggcctgg gcggaaaccg ggggcatggg actggcagtt cgccaagctc ctctgatcat
     5041 acctctgaaa gcaacctcta cccccgtgtc cataaaacaa taccccatgt cacaagaagc
     5101 cagactgggg atcaagcccc acatacagag actgttggac cagggaatac tggtaccctg
     5161 ccagtccccc tggaacacgc ccctgctacc cgttaagaaa ccagggacta atgattatag
     5221 gcctgtccag gatctgagag aagtcaacaa gcgggtggaa gacatccacc ccaccgtgcc
     5281 caacccttac aacctcttga gcgggctccc accgtcccac cagtggtaca ctgtgcttga
     5341 tttaaaggat gcctttttct gcctgagact ccaccccacc agtcagcctc tcttcgcctt
     5401 tgagtggaga gatccagaga tgggaatctc aggacaattg acctggacca gactcccaca
     5461 gggtttcaaa aacagtccca ccctgtttaa tgaggcactg cacagagacc tagcagactt
     5521 ccggatccag cacccagact tgatcctgct acagtacgtg gatgacttac tgctggccgc
     5581 cacttctgag ctagactgcc aacaaggtac tcgggccctg ttacaaaccc tagggaacct
     5641 cgggtatcgg gcctcggcca agaaagccca aatttgccag aaacaggtca agtatctggg
     5701 gtatcttcta aaagagggtc agagatggct gactgaggcc agaaaagaga ctgtgatggg
     5761 gcagcctact ccgaagaccc ctcgacaact aagggagttc ctagggaagg caggcttctg
     5821 tcgcctcttc atccctgggt ttgcagaaat ggcagccccc ctgtaccctc tcaccaaacc
     5881 ggggactctg tttaattggg gcccagacca acaaaaggcc tatcaagaaa tcaagcaagc
     5941 tcttctaact gccccagccc tggggttgcc agatttgact aagccctttg aactctttgt
     6001 cgacgagaag cagggctacg ccaaaggtgt cctaacgcaa aaactgggac cttggcgtcg
     6061 gccggtggcc tacctgtcca aaaagctaga cccagtagca gctgggtggc ccccttgcct
     6121 acggatggta gcagccattg ccgtactgac aaaggatgca ggcaagctaa ccatgggaca
     6181 gccactagtc attctggccc cccatgcagt agaggcacta gtcaaacaac cccccgaccg
     6241 ctggctttcc aacgcccgga tgactcacta tcaggccttg cttttggaca cggaccgggt
     6301 ccagttcgga ccggtggtag ccctgaaccc ggctacgctg ctcccactgc ctgaggaagg
     6361 gctgcaacac aactgccttg atatcctggc cgaagcccac ggaacccgac ccgacctaac
     6421 ggaccagccg ctcccagacg ccgaccacac ctggtacacg gatggaagca gtctcttaca
     6481 agagggacag cgtaaggcgg gagctgcggt gaccaccgag accgaggtaa tctgggctaa
     6541 agccctgcca gccgggacat ccgctcagcg ggctgaactg atagcactca cccaggccct
     6601 aaagatggca gaaggtaaga agctaaatgt ttatactgat agccgttatg cttttgctac
     6661 tgcccatatc catggagaaa tatacagaag gcgtgggtgg ctcacatcag aaggcaaaga
     6721 gatcaaaaat aaagacgaga tcttggccct actaaaagcc ctctttctgc ccaaaagact
     6781 tagcataatc cattgtccag gacatcaaaa gggacacagc gccgaggcta gaggcaaccg
     6841 gatggctgac caagcggccc gaaaggcagc catcacagag actccagaca cctctaccct
     6901 cctcatagaa aattcatcac cctctggcgg ctcaaaaaga accgccgacg gcagcgaatt
     6961 cgagcccaag aagaagagga aagtctaaac gcgttaagtc gacaatcaac ctctggatta
     7021 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     7081 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     7141 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     7201 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     7261 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     7321 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     7381 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     7441 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     7501 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     7561 gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag
     7621 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     7681 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     7741 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7801 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7861 ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc
     7921 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     7981 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     8041 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     8101 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     8161 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     8221 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     8281 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     8341 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     8401 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     8461 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     8521 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     8581 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     8641 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     8701 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     8761 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     8821 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     8881 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     8941 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     9001 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
     9061 acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg
     9121 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
     9181 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
     9241 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
     9301 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
     9361 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
     9421 gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt
     9481 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     9541 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     9601 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     9661 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     9721 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     9781 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     9841 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     9901 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     9961 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
    10021 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    10081 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    10141 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    10201 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    10261 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    10321 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    10381 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    10441 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    10501 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    10561 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
    10621 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    10681 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    10741 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    10801 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    10861 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    10921 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    10981 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    11041 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    11101 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    11161 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    11221 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    11281 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    11341 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    11401 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    11461 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    11521 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    11581 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    11641 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    11701 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    11761 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    11821 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    11881 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
    11941 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
    12001 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
    12061 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
    12121 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
    12181 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
    12241 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
    12301 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
    12361 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
    12421 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
    12481 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
    12541 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
    12601 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
    12661 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
    12721 tataagcagc gcgttttgcc tgtact
//
